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The map label for this gene is pksG [H]

Identifier: 187918539

GI number: 187918539

Start: 730208

End: 731464

Strand: Direct

Name: pksG [H]

Synonym: BH0683

Alternate gene names: 187918539

Gene position: 730208-731464 (Clockwise)

Preceding gene: 187918537

Following gene: 187918540

Centisome position: 79.17

GC content: 32.14

Gene sequence:

>1257_bases
TTGGCAATCATTTTTTTTAATTGGAGGAAATCGATGAGAGTAGGTATTAGTGATATTAGAGTTTTTTTACCTTTAAATTA
TTTAAATTTTAATGTTCTTTTAAGCAATTCTTTATATAAGTCTGATGGGATTTTTTTTAAGAAATTTAATAGAGCAATTG
ATGCAACCCTTCAGAAGGCATTTAGATTTACAAGTCCTAATGAAGACAGTGTTACAATGGCTAGTTCTGCTGTTAAGCTA
ATTTTTGATAATAATAATCTTAAGTTAGATAAGATGAGGATATTTTTAGGCGGAACTGAGACGGGGGTGGATTATTCAAA
GTCAATCTCATCTTATGTCTATGGGGCTTTGAAGTTGGCTGGCATTGATTTAAAAAATAATTTCTTGACTTTTCAAGCTC
AGCATGCATGTGCAGGTTCTGCGCTTGCTTTGCACAGTGCCGCAAGTATTTTGAGTCAGTTAGATAGGTCTGAATATGGC
ATAGTATTCTCTAGTGATATTGCACATTATAATAATCTTACAACAGCAGAGATTACTCAAGGGGCTGGAGCAACAGCAGT
ACTTATCGAACAAAATCCAAAAGTTTTATCTATTAATTTGTCTGAATTTGGGGTTTATACTGATGATGTTGATGATTTCT
TTAGACCATTTGGAAGTCTTGAGGCTAAGGTAAAGGGACGTTATTCTATTGAATGCTATAATAAGGCAAATGAAGAAGCT
TTAGCAGATTTTGCATATAAGAAAAATATGAGTATTAAGGATTTATTTTCTAACTATAGATTTATTTTGCATGTACCTTT
TGCAAAAATGCCTATAGATTCAATGCATTATGTTTTAAAGAAATATTATAGTGAAGATGAATCTGAGCGAAATGCTTATT
TAGAATCAATAGACTTTTATGATTCTGTTGAGGCTTCTAAGGAAGTGGGAAATTTATATACAGGGTCGATATTTTTATCT
TTAATGTCTTATTTGAAACGAGTATTTGCAAAAAAGGATATTAGTGGGGAGAAAATACTTTTTTGTTCTTATGGATCTGG
TAATATTATGGTTATTTATGAGCTTACAGTTGAAGATCATGCTCAATATGTTGTTAAGACTTGGGATATTGATAAAATAC
TCTCAGTAAGGCATAATGCAAGCTTTGATGAGTATAGAGATTTTTTTGAAAATAAGATCGCGCCAGGTGAATCCCAAGGC
TTCTATTTAAAGGAAATTAGAGAGGATGGATACCGAGTTTATGGGTATCGAGCCTAA

Upstream 100 bases:

>100_bases
TGTCTACGCCACCAGATAGAAGTACTGCTATTTTCATGATTTGCATCTCCTTTCAATATTATATTGAACTTTGCTGTTTT
TATGATCTATAGTATAATAC

Downstream 100 bases:

>100_bases
TATATTAAATAATAAAAAACGACAGATTGAAATTTGTTTAAATAAAGAAGATGTTAGCAAGAGTGATAATCTTTTAAATT
TTGTTAATTTAAAGCATGAT

Product: hydroxymethylglutaryl-CoA synthase

Products: (S)-3-hydroxy-3-methylglutaryl-CoA; CoA

Alternate protein names: HMG synthase [H]

Number of amino acids: Translated: 418; Mature: 417

Protein sequence:

>418_residues
MAIIFFNWRKSMRVGISDIRVFLPLNYLNFNVLLSNSLYKSDGIFFKKFNRAIDATLQKAFRFTSPNEDSVTMASSAVKL
IFDNNNLKLDKMRIFLGGTETGVDYSKSISSYVYGALKLAGIDLKNNFLTFQAQHACAGSALALHSAASILSQLDRSEYG
IVFSSDIAHYNNLTTAEITQGAGATAVLIEQNPKVLSINLSEFGVYTDDVDDFFRPFGSLEAKVKGRYSIECYNKANEEA
LADFAYKKNMSIKDLFSNYRFILHVPFAKMPIDSMHYVLKKYYSEDESERNAYLESIDFYDSVEASKEVGNLYTGSIFLS
LMSYLKRVFAKKDISGEKILFCSYGSGNIMVIYELTVEDHAQYVVKTWDIDKILSVRHNASFDEYRDFFENKIAPGESQG
FYLKEIREDGYRVYGYRA

Sequences:

>Translated_418_residues
MAIIFFNWRKSMRVGISDIRVFLPLNYLNFNVLLSNSLYKSDGIFFKKFNRAIDATLQKAFRFTSPNEDSVTMASSAVKL
IFDNNNLKLDKMRIFLGGTETGVDYSKSISSYVYGALKLAGIDLKNNFLTFQAQHACAGSALALHSAASILSQLDRSEYG
IVFSSDIAHYNNLTTAEITQGAGATAVLIEQNPKVLSINLSEFGVYTDDVDDFFRPFGSLEAKVKGRYSIECYNKANEEA
LADFAYKKNMSIKDLFSNYRFILHVPFAKMPIDSMHYVLKKYYSEDESERNAYLESIDFYDSVEASKEVGNLYTGSIFLS
LMSYLKRVFAKKDISGEKILFCSYGSGNIMVIYELTVEDHAQYVVKTWDIDKILSVRHNASFDEYRDFFENKIAPGESQG
FYLKEIREDGYRVYGYRA
>Mature_417_residues
AIIFFNWRKSMRVGISDIRVFLPLNYLNFNVLLSNSLYKSDGIFFKKFNRAIDATLQKAFRFTSPNEDSVTMASSAVKLI
FDNNNLKLDKMRIFLGGTETGVDYSKSISSYVYGALKLAGIDLKNNFLTFQAQHACAGSALALHSAASILSQLDRSEYGI
VFSSDIAHYNNLTTAEITQGAGATAVLIEQNPKVLSINLSEFGVYTDDVDDFFRPFGSLEAKVKGRYSIECYNKANEEAL
ADFAYKKNMSIKDLFSNYRFILHVPFAKMPIDSMHYVLKKYYSEDESERNAYLESIDFYDSVEASKEVGNLYTGSIFLSL
MSYLKRVFAKKDISGEKILFCSYGSGNIMVIYELTVEDHAQYVVKTWDIDKILSVRHNASFDEYRDFFENKIAPGESQGF
YLKEIREDGYRVYGYRA

Specific function: Involved in some intermediate steps for the synthesis of the antibiotic polyketide bacillaene which is involved in secondary metabolism. It catalyzes the aldol condensation between the acetyl group attached to the acyl-carrier-protein AcpK (Ac- AcpK) and

COG id: COG3425

COG function: function code I; 3-hydroxy-3-methylglutaryl CoA synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA synthase family [H]

Homologues:

Organism=Homo sapiens, GI5031751, Length=483, Percent_Identity=25.6728778467909, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI148298764, Length=403, Percent_Identity=26.0545905707196, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI148298677, Length=403, Percent_Identity=26.0545905707196, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17560126, Length=413, Percent_Identity=27.3607748184019, Blast_Score=98, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6323509, Length=428, Percent_Identity=24.5327102803738, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24654139, Length=474, Percent_Identity=26.3713080168776, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24654137, Length=474, Percent_Identity=26.3713080168776, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24654133, Length=474, Percent_Identity=26.3713080168776, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24654135, Length=474, Percent_Identity=26.3713080168776, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17933694, Length=474, Percent_Identity=26.3713080168776, Blast_Score=87, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013746
- InterPro:   IPR013528
- InterPro:   IPR016039
- InterPro:   IPR016038 [H]

Pfam domain/function: PF08540 HMG_CoA_synt_C; PF01154 HMG_CoA_synt_N [H]

EC number: 2.3.3.10

Molecular weight: Translated: 47530; Mature: 47399

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIIFFNWRKSMRVGISDIRVFLPLNYLNFNVLLSNSLYKSDGIFFKKFNRAIDATLQKA
CEEEEEECCCCHHCCHHHEEEEEEEEEEEEEEEEECCEECCCCCHHHHHHHHHHHHHHHH
FRFTSPNEDSVTMASSAVKLIFDNNNLKLDKMRIFLGGTETGVDYSKSISSYVYGALKLA
HCCCCCCCCCEEEECCEEEEEEECCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHEEE
GIDLKNNFLTFQAQHACAGSALALHSAASILSQLDRSEYGIVFSSDIAHYNNLTTAEITQ
EEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCHHCCCCCCEEEEC
GAGATAVLIEQNPKVLSINLSEFGVYTDDVDDFFRPFGSLEAKVKGRYSIECYNKANEEA
CCCCEEEEEECCCCEEEEEHHHCCCCCCCHHHHHCCCCCCEEEECCEEEEEEECCCCHHH
LADFAYKKNMSIKDLFSNYRFILHVPFAKMPIDSMHYVLKKYYSEDESERNAYLESIDFY
HHHHHHHCCCCHHHHHCCCEEEEECCHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCH
DSVEASKEVGNLYTGSIFLSLMSYLKRVFAKKDISGEKILFCSYGSGNIMVIYELTVEDH
HHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEEEECCC
AQYVVKTWDIDKILSVRHNASFDEYRDFFENKIAPGESQGFYLKEIREDGYRVYGYRA
CEEEEEECCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCCEEEEEEEC
>Mature Secondary Structure 
AIIFFNWRKSMRVGISDIRVFLPLNYLNFNVLLSNSLYKSDGIFFKKFNRAIDATLQKA
EEEEEECCCCHHCCHHHEEEEEEEEEEEEEEEEECCEECCCCCHHHHHHHHHHHHHHHH
FRFTSPNEDSVTMASSAVKLIFDNNNLKLDKMRIFLGGTETGVDYSKSISSYVYGALKLA
HCCCCCCCCCEEEECCEEEEEEECCCEEEEEEEEEECCCCCCCCHHHHHHHHHHHHHEEE
GIDLKNNFLTFQAQHACAGSALALHSAASILSQLDRSEYGIVFSSDIAHYNNLTTAEITQ
EEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCHHCCCCCCEEEEC
GAGATAVLIEQNPKVLSINLSEFGVYTDDVDDFFRPFGSLEAKVKGRYSIECYNKANEEA
CCCCEEEEEECCCCEEEEEHHHCCCCCCCHHHHHCCCCCCEEEECCEEEEEEECCCCHHH
LADFAYKKNMSIKDLFSNYRFILHVPFAKMPIDSMHYVLKKYYSEDESERNAYLESIDFY
HHHHHHHCCCCHHHHHCCCEEEEECCHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCH
DSVEASKEVGNLYTGSIFLSLMSYLKRVFAKKDISGEKILFCSYGSGNIMVIYELTVEDH
HHHHHHHHHCCEEHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEEEEEEECCC
AQYVVKTWDIDKILSVRHNASFDEYRDFFENKIAPGESQGFYLKEIREDGYRVYGYRA
CEEEEEECCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: acetyl-CoA; H2O; acetoacetyl-CoA

Specific reaction: acetyl-CoA + H2O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7704258; 9384377 [H]