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The map label for this gene is ptsI [H]

Identifier: 187918421

GI number: 187918421

Start: 578474

End: 580210

Strand: Direct

Name: ptsI [H]

Synonym: BH0558

Alternate gene names: 187918421

Gene position: 578474-580210 (Clockwise)

Preceding gene: 187918420

Following gene: 187918422

Centisome position: 62.72

GC content: 32.76

Gene sequence:

>1737_bases
ATGTGGAAGGTTGATATGACTTTATCGGGGAAAAGAATATCTAAAGGGATAGGTATAGGAGAAGCACTTTTTATTAAGAA
GGATTTTGATAAATTCATTGATAAATCAAAAATTGCTTCTTTTCAAATTGATAGTGAGATAAAAAAATTTAATGATGCTA
AGTCAAAGGCTATAGCGGTACTTAAAGATCTCACAAAAAAGGTTGTAGCTCAACTTGGGGCTGATAAGGAAGGCATTTTT
GAAGGGCAGATGTTAGTTATTGAGGATGATGAACTTTCTGATTCTGTTATAAGTTTTATTAAGAATAAAAATTATGGTGC
TGCTTATGCTGTATATCTATCCTTTAAAGAATTGATTGCAAGTGTAGAAGAATATACAGATGCTTATTTAAAAGAGCGAG
TTTCTGATTTTAAAGATATTAGGAATAGGCTTATTGCAAATATCTTAGATCAGGTGACTGATCTTGCTGAGGTTAAGAGA
GATGTAGTTCTGATTACTGAAGAATTAACACCTTCTGATACAATGCAGGTTGATTTAAATTATGTTAAGGGATTTGTGAC
CGCAGTTGGTGGTGAGACTTCTCATGCTGCTATTTTGGCAAGGACAATGGGACTTCCAGCTCTTGTGATGACGCCTTTAG
ATATTGCTAAAATTAAAGAAGGTGATCATTTAATAATTGATGGCCTGTCTTCAATAATTATTTCTAGTCCTTCATCCAGT
GAACTTGATAAATATACACATAAGATATTGGAGCATAATGAGCATGAAAAACAGCTTTTTGAATTAAAAAACCAGGATGC
AAAGACAAAAGACGGTATTAAAATATCTCTTAAGGCTAACATTGGAACTCCTACAGATATTTCTTATGTTAATAAATATG
GTCTTGATGGTATAGGGCTTTTTAGGACAGAATTTTTATATATGGAGTCTGTAAAGCCGCCAACAGAAGATGAACAGTTT
GAAGCCTATAAGAGAGTTTTAGAAACTATTGAAAAGAAAGGTGTTGTTACTATTCGTACTCTTGATATTGGCGGAGATAA
AGAGATTCCATATTTTAATTTTCCAAAGGAAGATAATCCTTTTTTAGGTTATCGTGCTCTTAGAATGTATATGGACTATG
AGGATTTGATACAAAGTCAGTTTAATGCGATTTTTAGGGCTAGTCATTATGGCAAGATAAGAATCATGGTTCCTATGCTT
ACTAGATATGAGGAAATTGATATAATTAATTATTTTGTAGATAAAGCTAAAGAGAATTTAAAGTCTAGAAATTTGCCTTT
TGATGAAAATTTGGAAGTCGGGTGTATGATAGAAGTTCCTTCAGCGGCTTTGGTGGCTTCTGAGTTTGCTGACAAATTGG
ATTTCTTTAGTATTGGTACTAATGATTTAACCCAATATACTTTGGCTGTAGATCGGGGTAATCAGAAGATATCAAATTTA
TATGATAAATATAATCCTGCTGTCTTAAGGCTTATTAAGAATGTTCTTGATGCTGGTAATAGTTCTGGTATTGATGTTTC
TGTTTGTGGTGAGCTTGGGGGAGATGAGGCTGGAGCTTTGATTCTTATTGGGCTTGGATTTAGATCTTTAAGTATGGTTC
CTAGTGCTTCACTTCGAATTAAGTATTTGCTAAGACAGTATACAATTTCTGATTTAAGTGAATTGGCAAATAAAGTGTTA
AATAGTAAGTCAGAATCAGAGACTTTAAAATTTTTAGATAAATATATAGGAGATTAG

Upstream 100 bases:

>100_bases
ATTATCTTCTGGCAAGAAGCTTATGGTTTGTGCTCAGGGTGATGATGAAGAGAAGGCTGTTACTGAACTTGTTGAGCTCA
TTGAATCTTTTAAAGAGTAG

Downstream 100 bases:

>100_bases
TTTATGGGGTTTTTAGGTTTTTTTAAAAAGTCTGCTACTTTGGACTTAATAGCACCTGTTAGTGGGAAAGTTGTTTCAAT
TGATAAGGTGCCAGATGAGG

Product: phosphoenolpyruvate-protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 578; Mature: 578

Protein sequence:

>578_residues
MWKVDMTLSGKRISKGIGIGEALFIKKDFDKFIDKSKIASFQIDSEIKKFNDAKSKAIAVLKDLTKKVVAQLGADKEGIF
EGQMLVIEDDELSDSVISFIKNKNYGAAYAVYLSFKELIASVEEYTDAYLKERVSDFKDIRNRLIANILDQVTDLAEVKR
DVVLITEELTPSDTMQVDLNYVKGFVTAVGGETSHAAILARTMGLPALVMTPLDIAKIKEGDHLIIDGLSSIIISSPSSS
ELDKYTHKILEHNEHEKQLFELKNQDAKTKDGIKISLKANIGTPTDISYVNKYGLDGIGLFRTEFLYMESVKPPTEDEQF
EAYKRVLETIEKKGVVTIRTLDIGGDKEIPYFNFPKEDNPFLGYRALRMYMDYEDLIQSQFNAIFRASHYGKIRIMVPML
TRYEEIDIINYFVDKAKENLKSRNLPFDENLEVGCMIEVPSAALVASEFADKLDFFSIGTNDLTQYTLAVDRGNQKISNL
YDKYNPAVLRLIKNVLDAGNSSGIDVSVCGELGGDEAGALILIGLGFRSLSMVPSASLRIKYLLRQYTISDLSELANKVL
NSKSESETLKFLDKYIGD

Sequences:

>Translated_578_residues
MWKVDMTLSGKRISKGIGIGEALFIKKDFDKFIDKSKIASFQIDSEIKKFNDAKSKAIAVLKDLTKKVVAQLGADKEGIF
EGQMLVIEDDELSDSVISFIKNKNYGAAYAVYLSFKELIASVEEYTDAYLKERVSDFKDIRNRLIANILDQVTDLAEVKR
DVVLITEELTPSDTMQVDLNYVKGFVTAVGGETSHAAILARTMGLPALVMTPLDIAKIKEGDHLIIDGLSSIIISSPSSS
ELDKYTHKILEHNEHEKQLFELKNQDAKTKDGIKISLKANIGTPTDISYVNKYGLDGIGLFRTEFLYMESVKPPTEDEQF
EAYKRVLETIEKKGVVTIRTLDIGGDKEIPYFNFPKEDNPFLGYRALRMYMDYEDLIQSQFNAIFRASHYGKIRIMVPML
TRYEEIDIINYFVDKAKENLKSRNLPFDENLEVGCMIEVPSAALVASEFADKLDFFSIGTNDLTQYTLAVDRGNQKISNL
YDKYNPAVLRLIKNVLDAGNSSGIDVSVCGELGGDEAGALILIGLGFRSLSMVPSASLRIKYLLRQYTISDLSELANKVL
NSKSESETLKFLDKYIGD
>Mature_578_residues
MWKVDMTLSGKRISKGIGIGEALFIKKDFDKFIDKSKIASFQIDSEIKKFNDAKSKAIAVLKDLTKKVVAQLGADKEGIF
EGQMLVIEDDELSDSVISFIKNKNYGAAYAVYLSFKELIASVEEYTDAYLKERVSDFKDIRNRLIANILDQVTDLAEVKR
DVVLITEELTPSDTMQVDLNYVKGFVTAVGGETSHAAILARTMGLPALVMTPLDIAKIKEGDHLIIDGLSSIIISSPSSS
ELDKYTHKILEHNEHEKQLFELKNQDAKTKDGIKISLKANIGTPTDISYVNKYGLDGIGLFRTEFLYMESVKPPTEDEQF
EAYKRVLETIEKKGVVTIRTLDIGGDKEIPYFNFPKEDNPFLGYRALRMYMDYEDLIQSQFNAIFRASHYGKIRIMVPML
TRYEEIDIINYFVDKAKENLKSRNLPFDENLEVGCMIEVPSAALVASEFADKLDFFSIGTNDLTQYTLAVDRGNQKISNL
YDKYNPAVLRLIKNVLDAGNSSGIDVSVCGELGGDEAGALILIGLGFRSLSMVPSASLRIKYLLRQYTISDLSELANKVL
NSKSESETLKFLDKYIGD

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=571, Percent_Identity=42.7320490367776, Blast_Score=465, Evalue=1e-132,
Organism=Escherichia coli, GI1788726, Length=571, Percent_Identity=32.399299474606, Blast_Score=313, Evalue=3e-86,
Organism=Escherichia coli, GI48994992, Length=522, Percent_Identity=32.9501915708812, Blast_Score=305, Evalue=6e-84,
Organism=Escherichia coli, GI1789193, Length=531, Percent_Identity=31.638418079096, Blast_Score=261, Evalue=1e-70,
Organism=Escherichia coli, GI1787994, Length=465, Percent_Identity=27.5268817204301, Blast_Score=122, Evalue=7e-29,
Organism=Escherichia coli, GI226510935, Length=167, Percent_Identity=26.3473053892216, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 64791; Mature: 64791

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWKVDMTLSGKRISKGIGIGEALFIKKDFDKFIDKSKIASFQIDSEIKKFNDAKSKAIAV
CEEEEEEECCCHHHCCCCCCCEEEEEHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHH
LKDLTKKVVAQLGADKEGIFEGQMLVIEDDELSDSVISFIKNKNYGAAYAVYLSFKELIA
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCEEHHHHHHHHHHH
SVEEYTDAYLKERVSDFKDIRNRLIANILDQVTDLAEVKRDVVLITEELTPSDTMQVDLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEHH
YVKGFVTAVGGETSHAAILARTMGLPALVMTPLDIAKIKEGDHLIIDGLSSIIISSPSSS
HHHHHHHHCCCCCCHHHHHHHHCCCCEEECCCCHHHHHCCCCEEEEECHHHHHCCCCCCH
ELDKYTHKILEHNEHEKQLFELKNQDAKTKDGIKISLKANIGTPTDISYVNKYGLDGIGL
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHHCCCCCCHH
FRTEFLYMESVKPPTEDEQFEAYKRVLETIEKKGVVTIRTLDIGGDKEIPYFNFPKEDNP
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCCC
FLGYRALRMYMDYEDLIQSQFNAIFRASHYGKIRIMVPMLTRYEEIDIINYFVDKAKENL
CHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEHHHHCHHHHHHHHHHHHHHHHHH
KSRNLPFDENLEVGCMIEVPSAALVASEFADKLDFFSIGTNDLTQYTLAVDRGNQKISNL
HHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCEEECCCCCCEEEEEEEECCCHHHHHH
YDKYNPAVLRLIKNVLDAGNSSGIDVSVCGELGGDEAGALILIGLGFRSLSMVPSASLRI
HHHCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEEECCCHHHHCCCCCHHHH
KYLLRQYTISDLSELANKVLNSKSESETLKFLDKYIGD
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MWKVDMTLSGKRISKGIGIGEALFIKKDFDKFIDKSKIASFQIDSEIKKFNDAKSKAIAV
CEEEEEEECCCHHHCCCCCCCEEEEEHHHHHHHHHHHHHEEEEHHHHHHHHHHHHHHHHH
LKDLTKKVVAQLGADKEGIFEGQMLVIEDDELSDSVISFIKNKNYGAAYAVYLSFKELIA
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCEEHHHHHHHHHHH
SVEEYTDAYLKERVSDFKDIRNRLIANILDQVTDLAEVKRDVVLITEELTPSDTMQVDLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEHH
YVKGFVTAVGGETSHAAILARTMGLPALVMTPLDIAKIKEGDHLIIDGLSSIIISSPSSS
HHHHHHHHCCCCCCHHHHHHHHCCCCEEECCCCHHHHHCCCCEEEEECHHHHHCCCCCCH
ELDKYTHKILEHNEHEKQLFELKNQDAKTKDGIKISLKANIGTPTDISYVNKYGLDGIGL
HHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCCCCHHHHHHCCCCCCHH
FRTEFLYMESVKPPTEDEQFEAYKRVLETIEKKGVVTIRTLDIGGDKEIPYFNFPKEDNP
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCCCCCCCCCCC
FLGYRALRMYMDYEDLIQSQFNAIFRASHYGKIRIMVPMLTRYEEIDIINYFVDKAKENL
CHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEHHHHCHHHHHHHHHHHHHHHHHH
KSRNLPFDENLEVGCMIEVPSAALVASEFADKLDFFSIGTNDLTQYTLAVDRGNQKISNL
HHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHCEEECCCCCCEEEEEEEECCCHHHHHH
YDKYNPAVLRLIKNVLDAGNSSGIDVSVCGELGGDEAGALILIGLGFRSLSMVPSASLRI
HHHCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCEEEEEECCCHHHHCCCCCHHHH
KYLLRQYTISDLSELANKVLNSKSESETLKFLDKYIGD
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]