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The map label for this gene is nadE2 [H]

Identifier: 187918386

GI number: 187918386

Start: 538358

End: 539926

Strand: Direct

Name: nadE2 [H]

Synonym: BH0522

Alternate gene names: 187918386

Gene position: 538358-539926 (Clockwise)

Preceding gene: 187918385

Following gene: 187918387

Centisome position: 58.37

GC content: 30.27

Gene sequence:

>1569_bases
TTGCTTATTATTTATTTTGTGAAAATATGTATAGCGCAGACAAAATGTAGACTTTTTAAGTTTAAACAGATTCTGAGTGA
GTTTAAGGAAAATTATGAGTATGCTATTCAAAATAAGGTTGATATTTTAGTTTTTCCTTTTATTTTTATTGGGGGCATTG
AGTACAAGAATTTATTTGATAGGGCAGAATATTTGAGCAAAATTCTTTATTGCCTTGATTTTATAAAAGAGCAAGTTGAT
GATAGACTTTGTGTTGTATTTGGACATTATAGTTTTTATGAGGGTAAATTATTAGATTGCATATCTGTAGTAAATAATCA
TCGGTTTGTCTTGACATCTAGGGAAGTCAATGTGCCGGTTTTATTTGATTATAAGGGTAAAAGTATTGCTGTATTAAATC
TGGGAGATGAGCTTTTATTTCAGGGATTTGATGAAAAATTTAGTGCGTCTTTTAGTAACATTGATTATCTCTTAATTCCA
TCAAAGTCTTATTTTACTGGAGATAAGAATAATTTGAGGCTTGAATTTTTCAAAAAAATAGCTGTTAAAAATAATTTGGA
AGTTGCTTATGCCAATTTATATGGTACTTATGATTCTATTGTATTTGATGGTTTGAGTTTTTTTATTAATAAATACGGTA
AAATAAAACAGGCTAGAGAATTTAAAGACGATATTTTATTTAATGAAAGATTTCATGATTTAGAGCTTGACTGTGAGTCT
AAAATTTTTGATAGACTTATTGAAGCATTGATAAGTAGTTTAAAAGAATATGTTAAGCTTTCTGGGTTTGATAAAGTTCA
TTTGGGTGTTTCTGGAGGCATTGATTCTGCTCTTGTTGCTTATATTGCATGTGTTGCTTTAGGGGCTCATAGAGTTGTTG
GCGTTTCTATGCCTAGTAGGTTTTCATCAAAAGGATCTGTTTTTGATGCAAAAGAACTTGCTAGAGAATTGGGATTTAAG
TTAATTGATATGCCTATTGAAAGCATGTTTCAATCTGCTTTAGAGTTTTTTGATGGGTATTTTGATACAAAGGGAGTTAC
TGAGGAAAATCTTCAAGCTAGACTTAGAGGGATTTTTTTAATGTCTTATAGTAATGCAAATAAATCTTTGCTTCTAAATA
CTGGAAATAAAAGTGAAATTGCTGTTGGTTATTGCACTCTTTATGGCGATTCTTGTGGTGGAATTGCTTTAATTGGTGAT
TTATTTAAGCGAGATGTTTATAGTCTTGCAAAATATATTAATTTAAAAGAAGGAAGATCAGTTATTCCTGTTGATATTCT
TTTAAAAGAACCTTCTGCCGAGCTTAGACCTGGTCAAAAAGACAGCGATTCTCTTCCTAGGTATGAAATTCTTGATGAGA
TATTAAGTCAATATTTACTTAAAAATGAGTCCTTGGAGTTGCTTTATGAATCTTTTGGAAAAGAAGTGGTTACAAAGGTG
TTAGATCTTTATTCTAGAAGTGAGTATAAAAGAAGGAAAGGCCCTATGATAGTTAAAGTTTCTAGAAAAGCTTTTGGTAA
TGACATTTTACTTCCTATTTCAAGAGTTGCGTTAACTGAGGATGAATAG

Upstream 100 bases:

>100_bases
GTAGGTAGGTATACTCCTAAGATAGCCCCAATTAAAATGAAAGATGATATTGAGAAATGGTTAAGTGTGATTTAGAGTTT
TCTTGTAATTTTTTCTGTTA

Downstream 100 bases:

>100_bases
TAATAATGTTATTAGTATATTAAATGAGTTTAGTTTAAAATTAGAAGAGATTTTCTTGCTTATCAATACTGATTCTTCTG
AACTTTACAAAGAGACTCCT

Product: glutamine-dependent NAD(+) synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 522; Mature: 522

Protein sequence:

>522_residues
MLIIYFVKICIAQTKCRLFKFKQILSEFKENYEYAIQNKVDILVFPFIFIGGIEYKNLFDRAEYLSKILYCLDFIKEQVD
DRLCVVFGHYSFYEGKLLDCISVVNNHRFVLTSREVNVPVLFDYKGKSIAVLNLGDELLFQGFDEKFSASFSNIDYLLIP
SKSYFTGDKNNLRLEFFKKIAVKNNLEVAYANLYGTYDSIVFDGLSFFINKYGKIKQAREFKDDILFNERFHDLELDCES
KIFDRLIEALISSLKEYVKLSGFDKVHLGVSGGIDSALVAYIACVALGAHRVVGVSMPSRFSSKGSVFDAKELARELGFK
LIDMPIESMFQSALEFFDGYFDTKGVTEENLQARLRGIFLMSYSNANKSLLLNTGNKSEIAVGYCTLYGDSCGGIALIGD
LFKRDVYSLAKYINLKEGRSVIPVDILLKEPSAELRPGQKDSDSLPRYEILDEILSQYLLKNESLELLYESFGKEVVTKV
LDLYSRSEYKRRKGPMIVKVSRKAFGNDILLPISRVALTEDE

Sequences:

>Translated_522_residues
MLIIYFVKICIAQTKCRLFKFKQILSEFKENYEYAIQNKVDILVFPFIFIGGIEYKNLFDRAEYLSKILYCLDFIKEQVD
DRLCVVFGHYSFYEGKLLDCISVVNNHRFVLTSREVNVPVLFDYKGKSIAVLNLGDELLFQGFDEKFSASFSNIDYLLIP
SKSYFTGDKNNLRLEFFKKIAVKNNLEVAYANLYGTYDSIVFDGLSFFINKYGKIKQAREFKDDILFNERFHDLELDCES
KIFDRLIEALISSLKEYVKLSGFDKVHLGVSGGIDSALVAYIACVALGAHRVVGVSMPSRFSSKGSVFDAKELARELGFK
LIDMPIESMFQSALEFFDGYFDTKGVTEENLQARLRGIFLMSYSNANKSLLLNTGNKSEIAVGYCTLYGDSCGGIALIGD
LFKRDVYSLAKYINLKEGRSVIPVDILLKEPSAELRPGQKDSDSLPRYEILDEILSQYLLKNESLELLYESFGKEVVTKV
LDLYSRSEYKRRKGPMIVKVSRKAFGNDILLPISRVALTEDE
>Mature_522_residues
MLIIYFVKICIAQTKCRLFKFKQILSEFKENYEYAIQNKVDILVFPFIFIGGIEYKNLFDRAEYLSKILYCLDFIKEQVD
DRLCVVFGHYSFYEGKLLDCISVVNNHRFVLTSREVNVPVLFDYKGKSIAVLNLGDELLFQGFDEKFSASFSNIDYLLIP
SKSYFTGDKNNLRLEFFKKIAVKNNLEVAYANLYGTYDSIVFDGLSFFINKYGKIKQAREFKDDILFNERFHDLELDCES
KIFDRLIEALISSLKEYVKLSGFDKVHLGVSGGIDSALVAYIACVALGAHRVVGVSMPSRFSSKGSVFDAKELARELGFK
LIDMPIESMFQSALEFFDGYFDTKGVTEENLQARLRGIFLMSYSNANKSLLLNTGNKSEIAVGYCTLYGDSCGGIALIGD
LFKRDVYSLAKYINLKEGRSVIPVDILLKEPSAELRPGQKDSDSLPRYEILDEILSQYLLKNESLELLYESFGKEVVTKV
LDLYSRSEYKRRKGPMIVKVSRKAFGNDILLPISRVALTEDE

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR000132
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 59561; Mature: 59561

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIIYFVKICIAQTKCRLFKFKQILSEFKENYEYAIQNKVDILVFPFIFIGGIEYKNLFD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHEEEHHHHHCCCHHHHHHH
RAEYLSKILYCLDFIKEQVDDRLCVVFGHYSFYEGKLLDCISVVNNHRFVLTSREVNVPV
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHCCCEEEEEECCCCCCE
LFDYKGKSIAVLNLGDELLFQGFDEKFSASFSNIDYLLIPSKSYFTGDKNNLRLEFFKKI
EEECCCCEEEEEECCHHHHHCCCCHHHCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHH
AVKNNLEVAYANLYGTYDSIVFDGLSFFINKYGKIKQAREFKDDILFNERFHDLELDCES
HHCCCCEEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
KIFDRLIEALISSLKEYVKLSGFDKVHLGVSGGIDSALVAYIACVALGAHRVVGVSMPSR
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCHHEEEECCCCC
FSSKGSVFDAKELARELGFKLIDMPIESMFQSALEFFDGYFDTKGVTEENLQARLRGIFL
CCCCCCCCCHHHHHHHCCCEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHEEEE
MSYSNANKSLLLNTGNKSEIAVGYCTLYGDSCGGIALIGDLFKRDVYSLAKYINLKEGRS
EEECCCCCEEEEECCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
VIPVDILLKEPSAELRPGQKDSDSLPRYEILDEILSQYLLKNESLELLYESFGKEVVTKV
CCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LDLYSRSEYKRRKGPMIVKVSRKAFGNDILLPISRVALTEDE
HHHHHHHHHHHHCCCEEEEEEHHHCCCCEEEEHHHEEECCCC
>Mature Secondary Structure
MLIIYFVKICIAQTKCRLFKFKQILSEFKENYEYAIQNKVDILVFPFIFIGGIEYKNLFD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHEEEHHHHHCCCHHHHHHH
RAEYLSKILYCLDFIKEQVDDRLCVVFGHYSFYEGKLLDCISVVNNHRFVLTSREVNVPV
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHHCCCEEEEEECCCCCCE
LFDYKGKSIAVLNLGDELLFQGFDEKFSASFSNIDYLLIPSKSYFTGDKNNLRLEFFKKI
EEECCCCEEEEEECCHHHHHCCCCHHHCCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHH
AVKNNLEVAYANLYGTYDSIVFDGLSFFINKYGKIKQAREFKDDILFNERFHDLELDCES
HHCCCCEEEEEEHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
KIFDRLIEALISSLKEYVKLSGFDKVHLGVSGGIDSALVAYIACVALGAHRVVGVSMPSR
HHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCHHEEEECCCCC
FSSKGSVFDAKELARELGFKLIDMPIESMFQSALEFFDGYFDTKGVTEENLQARLRGIFL
CCCCCCCCCHHHHHHHCCCEEECCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHEEEE
MSYSNANKSLLLNTGNKSEIAVGYCTLYGDSCGGIALIGDLFKRDVYSLAKYINLKEGRS
EEECCCCCEEEEECCCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
VIPVDILLKEPSAELRPGQKDSDSLPRYEILDEILSQYLLKNESLELLYESFGKEVVTKV
CCEEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
LDLYSRSEYKRRKGPMIVKVSRKAFGNDILLPISRVALTEDE
HHHHHHHHHHHHCCCEEEEEEHHHCCCCEEEEHHHEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10360571 [H]