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The map label for this gene is yfgF [H]

Identifier: 187918232

GI number: 187918232

Start: 374390

End: 376381

Strand: Direct

Name: yfgF [H]

Synonym: BH0363

Alternate gene names: 187918232

Gene position: 374390-376381 (Clockwise)

Preceding gene: 187918231

Following gene: 187918233

Centisome position: 40.59

GC content: 25.35

Gene sequence:

>1992_bases
ATGAAAAATCCAAATGCAATTGTAATATCTGAAGGGGCTATTAGCAATGATGATATTACAAAGATCAAATCTATTTTTAA
AATATTACAAATAATAAAATCTAGAAAAAATATCTCTAAGGAATATATTAAACAAAGTAATATTAAGTTTGCTATTATTT
ACAACCATAAAAGACCAATAGATTTTTCAATCAATATAGCAAATGACTTAAAAAGCATTAATAACGTTCATTCTATCATT
ATAAACAATAAATCTATAGACAAAGCAATGTATAGGCCAAATTACATAGAGATATTAAACAACATCAGCGAATTAAGCGA
TAAACATAAATTAATACAACAGAAAAAACTTTATTATGACAATAATAATGCTAATCTTGATTTTTTCTTAAATCTATCTG
AGCTTATTCAAGAAATCGTGATCATTACAAATACCGACAATGATATCATCTATATTAATGAAAAAGGCAGCAAAGAACTT
GAACTCCCAATAAAAATCAGAGGAAAAACAAACAAAATAAGTGATATCAATATCATAGATTTAGAAAAAGAAACTAAAAT
AGATTTAAGTTACAACATAAATAACATTCCTGAATTCAAAAATATACTGATAACCGACTGCCTTTTAAAGGTTAAAAATA
ACAAAAAATTAATCGTCGATTTATTCATCAGTACAATTGCTCAAAACAACATTGATAAATTAATTACAATAAAAGACATA
TCAGACTTAAAATCTAAAAACAATATTCAGGATCTCGAAATCATTGATCAACAAACAGATCTATATAACATTAAAGGACT
TGAAAAACTATTAATCAACCAAATTGAATCCTCTCATAAAAAGATATATTTATTTGATTTAGATTTACACATAAATACAG
AATATGAATATAAAGGCAATAAAGACAACTTAGATTCAAAAATACTTAAGAAAATTACTTCTAGAATAATGTCATTCTAT
TCAGAATACATATTTAGACTCAAAGACAATAATTTAATAGTCATTATATCCACAAGTGGGGGGGAGCAAAGAATAATTTC
AATTGCGGAAGAAATCAAAAAAACCATCTCCAGAGAACTTAGCAAAGAAGGCATCATAATATTCAAACTCAACATAGGAA
TAATAGAAGTAAATTTAAAAGAAGATATAGAGACAAAAATTTCAAAATTAAAAATAGCAACCAAAATATCTGATGAATAT
AAAGATTCTACGCCTATTTTATACAAAGATGAATTACCAGAAACAATACTTATTAAAAATCAAAATAAAATATTTGAATA
TATAGTAAAAGCAATAAAGAATGATTTTTTTAGTCTCTACTATCAAAAAATTACTCCTCTTAAAAAAGATCTAAAACCCA
AAATTGAAATATTAACAAGACTTTTTGACTATACAGGGACTCCCATCCCAAACGACAATGTCTTTAGCTTAATAGATAAG
TATAATCTAACTGTTGAAGTGGATAAATTGGTAGTTACTAAAACCTTAAGAGAATATACAAATTTTGTGGCAAAAAATGG
TGTTCATATCTTTTCAATCAATATTTCACCATACTCACTTAAATCCAAAAACTTCAGAATGTTTCTAAAAGAAACACTTC
TTAAAAGTCATGTCCCACTTCAAAACATATGCTTAGAAATAACAGAAACTGGGATTTTAGAAAATTTTGAATTAATCAAA
ACATATTTTAATGAACTTAAAACTTTCGGGGTTAAACTTGCACTTGACGATTTTGGTAGTGGATATACATCACTCTCATA
TATTAAAATCTTGCCAATAGATATTATCAAAATAGATGGTTCTTTTATTCAAGTAATAAATTCTAGTCAAATAGACCTTG
TAATAATAAAATCAATAAAAGACATTGCTGATACAAAAAACATAAAAATTATAGCTGAATTTGTATCAAATGAAGAAATA
CTTAAGAAAATAAACGAGATCGGAATAGATTATGGACAAGGATTCCTATGGCATAAACCAGAACCTATTTAA

Upstream 100 bases:

>100_bases
AATATGGCTCGCATGGGCTAGAACGAGACCAGAAAAACTAAAGAAATAAATATCAAAATATAGATTTATATCAAAATTAA
TATAAATTTATTTAAATAAG

Downstream 100 bases:

>100_bases
AAATACACAAATGTTTTTTAATACTAGTGTAGCTCTAGTATAGATTAGGCCGCATAAAGGACACTGCAAAATTAATCCAT
TGCAGCTGTTTTTCATTATT

Product: sensory box/GGDEF family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 663; Mature: 663

Protein sequence:

>663_residues
MKNPNAIVISEGAISNDDITKIKSIFKILQIIKSRKNISKEYIKQSNIKFAIIYNHKRPIDFSINIANDLKSINNVHSII
INNKSIDKAMYRPNYIEILNNISELSDKHKLIQQKKLYYDNNNANLDFFLNLSELIQEIVIITNTDNDIIYINEKGSKEL
ELPIKIRGKTNKISDINIIDLEKETKIDLSYNINNIPEFKNILITDCLLKVKNNKKLIVDLFISTIAQNNIDKLITIKDI
SDLKSKNNIQDLEIIDQQTDLYNIKGLEKLLINQIESSHKKIYLFDLDLHINTEYEYKGNKDNLDSKILKKITSRIMSFY
SEYIFRLKDNNLIVIISTSGGEQRIISIAEEIKKTISRELSKEGIIIFKLNIGIIEVNLKEDIETKISKLKIATKISDEY
KDSTPILYKDELPETILIKNQNKIFEYIVKAIKNDFFSLYYQKITPLKKDLKPKIEILTRLFDYTGTPIPNDNVFSLIDK
YNLTVEVDKLVVTKTLREYTNFVAKNGVHIFSINISPYSLKSKNFRMFLKETLLKSHVPLQNICLEITETGILENFELIK
TYFNELKTFGVKLALDDFGSGYTSLSYIKILPIDIIKIDGSFIQVINSSQIDLVIIKSIKDIADTKNIKIIAEFVSNEEI
LKKINEIGIDYGQGFLWHKPEPI

Sequences:

>Translated_663_residues
MKNPNAIVISEGAISNDDITKIKSIFKILQIIKSRKNISKEYIKQSNIKFAIIYNHKRPIDFSINIANDLKSINNVHSII
INNKSIDKAMYRPNYIEILNNISELSDKHKLIQQKKLYYDNNNANLDFFLNLSELIQEIVIITNTDNDIIYINEKGSKEL
ELPIKIRGKTNKISDINIIDLEKETKIDLSYNINNIPEFKNILITDCLLKVKNNKKLIVDLFISTIAQNNIDKLITIKDI
SDLKSKNNIQDLEIIDQQTDLYNIKGLEKLLINQIESSHKKIYLFDLDLHINTEYEYKGNKDNLDSKILKKITSRIMSFY
SEYIFRLKDNNLIVIISTSGGEQRIISIAEEIKKTISRELSKEGIIIFKLNIGIIEVNLKEDIETKISKLKIATKISDEY
KDSTPILYKDELPETILIKNQNKIFEYIVKAIKNDFFSLYYQKITPLKKDLKPKIEILTRLFDYTGTPIPNDNVFSLIDK
YNLTVEVDKLVVTKTLREYTNFVAKNGVHIFSINISPYSLKSKNFRMFLKETLLKSHVPLQNICLEITETGILENFELIK
TYFNELKTFGVKLALDDFGSGYTSLSYIKILPIDIIKIDGSFIQVINSSQIDLVIIKSIKDIADTKNIKIIAEFVSNEEI
LKKINEIGIDYGQGFLWHKPEPI
>Mature_663_residues
MKNPNAIVISEGAISNDDITKIKSIFKILQIIKSRKNISKEYIKQSNIKFAIIYNHKRPIDFSINIANDLKSINNVHSII
INNKSIDKAMYRPNYIEILNNISELSDKHKLIQQKKLYYDNNNANLDFFLNLSELIQEIVIITNTDNDIIYINEKGSKEL
ELPIKIRGKTNKISDINIIDLEKETKIDLSYNINNIPEFKNILITDCLLKVKNNKKLIVDLFISTIAQNNIDKLITIKDI
SDLKSKNNIQDLEIIDQQTDLYNIKGLEKLLINQIESSHKKIYLFDLDLHINTEYEYKGNKDNLDSKILKKITSRIMSFY
SEYIFRLKDNNLIVIISTSGGEQRIISIAEEIKKTISRELSKEGIIIFKLNIGIIEVNLKEDIETKISKLKIATKISDEY
KDSTPILYKDELPETILIKNQNKIFEYIVKAIKNDFFSLYYQKITPLKKDLKPKIEILTRLFDYTGTPIPNDNVFSLIDK
YNLTVEVDKLVVTKTLREYTNFVAKNGVHIFSINISPYSLKSKNFRMFLKETLLKSHVPLQNICLEITETGILENFELIK
TYFNELKTFGVKLALDDFGSGYTSLSYIKILPIDIIKIDGSFIQVINSSQIDLVIIKSIKDIADTKNIKIIAEFVSNEEI
LKKINEIGIDYGQGFLWHKPEPI

Specific function: Truncated proteins consisting of the GGDEF/EAL domains (residues 319-747) or of the EAL domain alone (481-747) have c-di- GMP phosphodiesterase activity. They do not have diguanylate cyclase activity. Cyclic-di-GMP is a second messenger which controls cel

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1788849, Length=431, Percent_Identity=25.5220417633411, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI87082096, Length=208, Percent_Identity=33.6538461538462, Blast_Score=121, Evalue=2e-28,
Organism=Escherichia coli, GI1787541, Length=460, Percent_Identity=24.3478260869565, Blast_Score=109, Evalue=6e-25,
Organism=Escherichia coli, GI226510982, Length=239, Percent_Identity=27.6150627615063, Blast_Score=99, Evalue=8e-22,
Organism=Escherichia coli, GI1788502, Length=238, Percent_Identity=28.1512605042017, Blast_Score=96, Evalue=9e-21,
Organism=Escherichia coli, GI87081980, Length=227, Percent_Identity=28.6343612334802, Blast_Score=95, Evalue=2e-20,
Organism=Escherichia coli, GI87081921, Length=461, Percent_Identity=21.9088937093275, Blast_Score=93, Evalue=6e-20,
Organism=Escherichia coli, GI87081845, Length=240, Percent_Identity=30, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI87081743, Length=235, Percent_Identity=27.6595744680851, Blast_Score=90, Evalue=4e-19,
Organism=Escherichia coli, GI1790496, Length=233, Percent_Identity=26.1802575107296, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1786507, Length=244, Percent_Identity=27.8688524590164, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1787055, Length=184, Percent_Identity=28.2608695652174, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1789650, Length=295, Percent_Identity=18.3050847457627, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000160
- InterPro:   IPR001633
- InterPro:   IPR007895 [H]

Pfam domain/function: PF00563 EAL; PF05231 MASE1 [H]

EC number: =3.1.4.52 [H]

Molecular weight: Translated: 76618; Mature: 76618

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: PS50883 EAL ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNPNAIVISEGAISNDDITKIKSIFKILQIIKSRKNISKEYIKQSNIKFAIIYNHKRPI
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCE
DFSINIANDLKSINNVHSIIINNKSIDKAMYRPNYIEILNNISELSDKHKLIQQKKLYYD
EEEEEEHHHHHHHCCEEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEE
NNNANLDFFLNLSELIQEIVIITNTDNDIIYINEKGSKELELPIKIRGKTNKISDINIID
CCCCCEEEEECHHHHHHEEEEEECCCCCEEEEECCCCCEEEEEEEEECCCCCCCCEEEEE
LEKETKIDLSYNINNIPEFKNILITDCLLKVKNNKKLIVDLFISTIAQNNIDKLITIKDI
CCCCCEEEEEECCCCCCHHHHHHHHHHHHEECCCCEEEHHHHHHHHHCCCCCCEEEEECH
SDLKSKNNIQDLEIIDQQTDLYNIKGLEKLLINQIESSHKKIYLFDLDLHINTEYEYKGN
HHHHCCCCCCHHHEECCCCCEECHHHHHHHHHHHHHCCCCEEEEEEEEEEEECEEEECCC
KDNLDSKILKKITSRIMSFYSEYIFRLKDNNLIVIISTSGGEQRIISIAEEIKKTISREL
CCCHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
SKEGIIIFKLNIGIIEVNLKEDIETKISKLKIATKISDEYKDSTPILYKDELPETILIKN
CCCCEEEEEEEEEEEEEECHHHHHHHHHHHEEEEHHCHHCCCCCCEEEECCCCCEEEEEC
QNKIFEYIVKAIKNDFFSLYYQKITPLKKDLKPKIEILTRLFDYTGTPIPNDNVFSLIDK
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHH
YNLTVEVDKLVVTKTLREYTNFVAKNGVHIFSINISPYSLKSKNFRMFLKETLLKSHVPL
CCCEEEEHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEECCCCHHHHHHHHHHHHCCCH
QNICLEITETGILENFELIKTYFNELKTFGVKLALDDFGSGYTSLSYIKILPIDIIKIDG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEEEEEEEEEEEECC
SFIQVINSSQIDLVIIKSIKDIADTKNIKIIAEFVSNEEILKKINEIGIDYGQGFLWHKP
CEEEEECCCCEEEEEEEHHHHHHCCCCEEEEEEHHCCHHHHHHHHHCCCCCCCCCCCCCC
EPI
CCC
>Mature Secondary Structure
MKNPNAIVISEGAISNDDITKIKSIFKILQIIKSRKNISKEYIKQSNIKFAIIYNHKRPI
CCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCE
DFSINIANDLKSINNVHSIIINNKSIDKAMYRPNYIEILNNISELSDKHKLIQQKKLYYD
EEEEEEHHHHHHHCCEEEEEECCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHEEE
NNNANLDFFLNLSELIQEIVIITNTDNDIIYINEKGSKELELPIKIRGKTNKISDINIID
CCCCCEEEEECHHHHHHEEEEEECCCCCEEEEECCCCCEEEEEEEEECCCCCCCCEEEEE
LEKETKIDLSYNINNIPEFKNILITDCLLKVKNNKKLIVDLFISTIAQNNIDKLITIKDI
CCCCCEEEEEECCCCCCHHHHHHHHHHHHEECCCCEEEHHHHHHHHHCCCCCCEEEEECH
SDLKSKNNIQDLEIIDQQTDLYNIKGLEKLLINQIESSHKKIYLFDLDLHINTEYEYKGN
HHHHCCCCCCHHHEECCCCCEECHHHHHHHHHHHHHCCCCEEEEEEEEEEEECEEEECCC
KDNLDSKILKKITSRIMSFYSEYIFRLKDNNLIVIISTSGGEQRIISIAEEIKKTISREL
CCCHHHHHHHHHHHHHHHHHHHHHEEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
SKEGIIIFKLNIGIIEVNLKEDIETKISKLKIATKISDEYKDSTPILYKDELPETILIKN
CCCCEEEEEEEEEEEEEECHHHHHHHHHHHEEEEHHCHHCCCCCCEEEECCCCCEEEEEC
QNKIFEYIVKAIKNDFFSLYYQKITPLKKDLKPKIEILTRLFDYTGTPIPNDNVFSLIDK
CHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHH
YNLTVEVDKLVVTKTLREYTNFVAKNGVHIFSINISPYSLKSKNFRMFLKETLLKSHVPL
CCCEEEEHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCEECCCCHHHHHHHHHHHHCCCH
QNICLEITETGILENFELIKTYFNELKTFGVKLALDDFGSGYTSLSYIKILPIDIIKIDG
HHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCEEEEEEEEEEEEEEECC
SFIQVINSSQIDLVIIKSIKDIADTKNIKIIAEFVSNEEILKKINEIGIDYGQGFLWHKP
CEEEEECCCCEEEEEEEHHHHHHCCCCEEEEEEHHCCHHHHHHHHHCCCCCCCCCCCCCC
EPI
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]