Click here to switch to the map view.

The map label for this gene is pyk [H]

Identifier: 187918218

GI number: 187918218

Start: 360376

End: 361809

Strand: Direct

Name: pyk [H]

Synonym: BH0348

Alternate gene names: 187918218

Gene position: 360376-361809 (Clockwise)

Preceding gene: 187918217

Following gene: 187918219

Centisome position: 39.07

GC content: 33.19

Gene sequence:

>1434_bases
ATGATACAAAAATTAACAAAAATAGTAGCAACAATATCTGACCTCAGATGTGATCCAGAACATATAAAAGAGTTATATGA
AGCAGGGGTAAATGTAATAAGACTTAACACTGCACATCAATCTCATGACGATGCAATAAAAGTAATAAACAATGTTAGGC
AAGTTTCAAATAAAATAGCATTAATGATTGATACAAAAGGACCAGAAGTTAGAACAGCCAATATTGAAAATCCCATTACT
GTGAAGATAGGAGACAAAATAATAATCTCAACATCACCTATCAATGAATCTAATTCATTGCAAACCAACTATGAGGGCTT
TGTAAATGAAGTTCCAAATGGATCAAAAATCCTTATTGATGATGGCGAACTTGAAATGATTGTCATCGAAAAACTCGCAG
ACAGATTAATCTGTGAAGTTAAAAATGATGGTCATATCAAAAACAAAAAATCAATCAATACACCAGGAATTCCACTTAAA
CTACAATCTGTCACTGAAAAGGATAAGGGATTTATTGAACTTGCCGCAAAACAAAATATTGATTTTATTGCCCACTCATT
TGTAAGACATGCAAAAGACATTCAAGACGTTAAAGATATATTAAATACTGCTGGAAATCCAGATGTCAAAATTATTGCCA
AGGTTGAAAATCAAGAAGGAATTGATAATATTGAAGAGATTGCAAAGGCTTCTTATGGGATTATGGTTGCAAGGGGCGAT
ATGGGAGTCGAAATACCTGCTGAAGATGTTCCTTTGGCACAAATTAAAATAACAAAAACCTGCATTAAATATGGAATACC
CGTAATTACCGCAACACAAATGCTGCACACAATGATTGAAAATCCAAGACCTACAAGAGCAGAGGTTTCCGATGTTGCTA
ATGCAATTCTAAATGGCACAGATGCCATAATGTTATCTGGTGAGACAGCTTATGGCAAATACCCACTCGAAGCTGTTAAG
ATGATGACTAAAATTGCTATAGAGGTTGAAAAATATAGAGAAAAAACACTATTTAAAGATGAAATTTTCTGCAGCAAAAA
GATCATAAGAAACTATATTATTAAATGTGCAATTGATGCAACCAAGATAATGCCTGTTAAAGCTATTATTGTTGATTCAC
TTAAGGGAAGAACCGCAAGAATAATGGCAACATACAGAGCAAGTGTACCTCTATTTATTACAACAAATAATGAAAGGATA
GCAAGGGAATTATCACTTTCTTACGGTGTTTATTCTAATCTTGTCGATCATAACTTCAAGGGAACAACTGAATTCGTAGT
AACTTCTCTTGAAATGCTGAAAACACAAGGCATAGTTAAAGATTCAGATATTGTAGTAATTATCTCTGGAAATCCAAACA
GAGCTACCAACAAAGGTACAGAATTTATGGAAATAAATACAGTAGAAGACGCAATTAAGGAACGAAACCTATAA

Upstream 100 bases:

>100_bases
TATGATAATTTAATCTTAAAATGAAATGACAAGTTCTTAAAAAAATATTGTTATTGGTGATTAAAAAGCATAAAATTTAA
ATACTGTAGGAGAAAAATAA

Downstream 100 bases:

>100_bases
AGAAAATAAGAAACAGTTTGGTTGATAATATATTTTATTCAACTAATACTTGCAACCCAGAATTATTTAATCTAAATAAA
AGAAAAACTCATAAAGCACT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 477; Mature: 477

Protein sequence:

>477_residues
MIQKLTKIVATISDLRCDPEHIKELYEAGVNVIRLNTAHQSHDDAIKVINNVRQVSNKIALMIDTKGPEVRTANIENPIT
VKIGDKIIISTSPINESNSLQTNYEGFVNEVPNGSKILIDDGELEMIVIEKLADRLICEVKNDGHIKNKKSINTPGIPLK
LQSVTEKDKGFIELAAKQNIDFIAHSFVRHAKDIQDVKDILNTAGNPDVKIIAKVENQEGIDNIEEIAKASYGIMVARGD
MGVEIPAEDVPLAQIKITKTCIKYGIPVITATQMLHTMIENPRPTRAEVSDVANAILNGTDAIMLSGETAYGKYPLEAVK
MMTKIAIEVEKYREKTLFKDEIFCSKKIIRNYIIKCAIDATKIMPVKAIIVDSLKGRTARIMATYRASVPLFITTNNERI
ARELSLSYGVYSNLVDHNFKGTTEFVVTSLEMLKTQGIVKDSDIVVIISGNPNRATNKGTEFMEINTVEDAIKERNL

Sequences:

>Translated_477_residues
MIQKLTKIVATISDLRCDPEHIKELYEAGVNVIRLNTAHQSHDDAIKVINNVRQVSNKIALMIDTKGPEVRTANIENPIT
VKIGDKIIISTSPINESNSLQTNYEGFVNEVPNGSKILIDDGELEMIVIEKLADRLICEVKNDGHIKNKKSINTPGIPLK
LQSVTEKDKGFIELAAKQNIDFIAHSFVRHAKDIQDVKDILNTAGNPDVKIIAKVENQEGIDNIEEIAKASYGIMVARGD
MGVEIPAEDVPLAQIKITKTCIKYGIPVITATQMLHTMIENPRPTRAEVSDVANAILNGTDAIMLSGETAYGKYPLEAVK
MMTKIAIEVEKYREKTLFKDEIFCSKKIIRNYIIKCAIDATKIMPVKAIIVDSLKGRTARIMATYRASVPLFITTNNERI
ARELSLSYGVYSNLVDHNFKGTTEFVVTSLEMLKTQGIVKDSDIVVIISGNPNRATNKGTEFMEINTVEDAIKERNL
>Mature_477_residues
MIQKLTKIVATISDLRCDPEHIKELYEAGVNVIRLNTAHQSHDDAIKVINNVRQVSNKIALMIDTKGPEVRTANIENPIT
VKIGDKIIISTSPINESNSLQTNYEGFVNEVPNGSKILIDDGELEMIVIEKLADRLICEVKNDGHIKNKKSINTPGIPLK
LQSVTEKDKGFIELAAKQNIDFIAHSFVRHAKDIQDVKDILNTAGNPDVKIIAKVENQEGIDNIEEIAKASYGIMVARGD
MGVEIPAEDVPLAQIKITKTCIKYGIPVITATQMLHTMIENPRPTRAEVSDVANAILNGTDAIMLSGETAYGKYPLEAVK
MMTKIAIEVEKYREKTLFKDEIFCSKKIIRNYIIKCAIDATKIMPVKAIIVDSLKGRTARIMATYRASVPLFITTNNERI
ARELSLSYGVYSNLVDHNFKGTTEFVVTSLEMLKTQGIVKDSDIVVIISGNPNRATNKGTEFMEINTVEDAIKERNL

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=471, Percent_Identity=38.0042462845011, Blast_Score=296, Evalue=3e-80,
Organism=Homo sapiens, GI33286420, Length=471, Percent_Identity=38.0042462845011, Blast_Score=296, Evalue=3e-80,
Organism=Homo sapiens, GI33286418, Length=471, Percent_Identity=38.2165605095541, Blast_Score=291, Evalue=7e-79,
Organism=Homo sapiens, GI10835121, Length=473, Percent_Identity=35.7293868921776, Blast_Score=284, Evalue=2e-76,
Organism=Homo sapiens, GI32967597, Length=473, Percent_Identity=35.7293868921776, Blast_Score=283, Evalue=2e-76,
Organism=Homo sapiens, GI310128732, Length=286, Percent_Identity=38.1118881118881, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI310128730, Length=286, Percent_Identity=38.1118881118881, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI310128736, Length=234, Percent_Identity=34.1880341880342, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI310128734, Length=234, Percent_Identity=34.1880341880342, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=33.953488372093, Blast_Score=123, Evalue=4e-28,
Organism=Escherichia coli, GI1787965, Length=466, Percent_Identity=39.4849785407725, Blast_Score=320, Evalue=2e-88,
Organism=Escherichia coli, GI1788160, Length=463, Percent_Identity=33.0453563714903, Blast_Score=228, Evalue=8e-61,
Organism=Caenorhabditis elegans, GI17544584, Length=464, Percent_Identity=36.6379310344828, Blast_Score=286, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI71984413, Length=471, Percent_Identity=35.8811040339703, Blast_Score=258, Evalue=4e-69,
Organism=Caenorhabditis elegans, GI17506829, Length=471, Percent_Identity=35.8811040339703, Blast_Score=258, Evalue=4e-69,
Organism=Caenorhabditis elegans, GI71984406, Length=490, Percent_Identity=35.5102040816327, Blast_Score=258, Evalue=4e-69,
Organism=Caenorhabditis elegans, GI17506831, Length=471, Percent_Identity=35.8811040339703, Blast_Score=258, Evalue=7e-69,
Organism=Saccharomyces cerevisiae, GI6319279, Length=487, Percent_Identity=35.3182751540041, Blast_Score=254, Evalue=1e-68,
Organism=Saccharomyces cerevisiae, GI6324923, Length=487, Percent_Identity=35.1129363449692, Blast_Score=251, Evalue=2e-67,
Organism=Drosophila melanogaster, GI24648964, Length=477, Percent_Identity=38.5744234800839, Blast_Score=298, Evalue=5e-81,
Organism=Drosophila melanogaster, GI28571814, Length=477, Percent_Identity=38.5744234800839, Blast_Score=298, Evalue=6e-81,
Organism=Drosophila melanogaster, GI24648966, Length=419, Percent_Identity=40.0954653937947, Blast_Score=292, Evalue=4e-79,
Organism=Drosophila melanogaster, GI24581235, Length=480, Percent_Identity=32.5, Blast_Score=234, Evalue=1e-61,
Organism=Drosophila melanogaster, GI24646914, Length=280, Percent_Identity=38.5714285714286, Blast_Score=181, Evalue=1e-45,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 52930; Mature: 52930

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIQKLTKIVATISDLRCDPEHIKELYEAGVNVIRLNTAHQSHDDAIKVINNVRQVSNKIA
CHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEE
LMIDTKGPEVRTANIENPITVKIGDKIIISTSPINESNSLQTNYEGFVNEVPNGSKILID
EEEECCCCCEEEECCCCCEEEEECCEEEEEECCCCCCCCCCCCCCHHHHCCCCCCEEEEE
DGELEMIVIEKLADRLICEVKNDGHIKNKKSINTPGIPLKLQSVTEKDKGFIELAAKQNI
CCCEEEEHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCEEECCCCCCCCCEEEEEECCCH
DFIAHSFVRHAKDIQDVKDILNTAGNPDVKIIAKVENQEGIDNIEEIAKASYGIMVARGD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCEEEEECC
MGVEIPAEDVPLAQIKITKTCIKYGIPVITATQMLHTMIENPRPTRAEVSDVANAILNGT
CCCCCCCCCCCEEEEEHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
DAIMLSGETAYGKYPLEAVKMMTKIAIEVEKYREKTLFKDEIFCSKKIIRNYIIKCAIDA
CEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
TKIMPVKAIIVDSLKGRTARIMATYRASVPLFITTNNERIARELSLSYGVYSNLVDHNFK
HHCCEEHHEEHHCCCCCEEEEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCC
GTTEFVVTSLEMLKTQGIVKDSDIVVIISGNPNRATNKGTEFMEINTVEDAIKERNL
CCHHHHHHHHHHHHHCCCEECCCEEEEEECCCCCCCCCCCEEEEECCHHHHHHHCCC
>Mature Secondary Structure
MIQKLTKIVATISDLRCDPEHIKELYEAGVNVIRLNTAHQSHDDAIKVINNVRQVSNKIA
CHHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEE
LMIDTKGPEVRTANIENPITVKIGDKIIISTSPINESNSLQTNYEGFVNEVPNGSKILID
EEEECCCCCEEEECCCCCEEEEECCEEEEEECCCCCCCCCCCCCCHHHHCCCCCCEEEEE
DGELEMIVIEKLADRLICEVKNDGHIKNKKSINTPGIPLKLQSVTEKDKGFIELAAKQNI
CCCEEEEHHHHHHHHHEEEECCCCCCCCCCCCCCCCCCEEECCCCCCCCCEEEEEECCCH
DFIAHSFVRHAKDIQDVKDILNTAGNPDVKIIAKVENQEGIDNIEEIAKASYGIMVARGD
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHCCCCEEEEECC
MGVEIPAEDVPLAQIKITKTCIKYGIPVITATQMLHTMIENPRPTRAEVSDVANAILNGT
CCCCCCCCCCCEEEEEHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCC
DAIMLSGETAYGKYPLEAVKMMTKIAIEVEKYREKTLFKDEIFCSKKIIRNYIIKCAIDA
CEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECC
TKIMPVKAIIVDSLKGRTARIMATYRASVPLFITTNNERIARELSLSYGVYSNLVDHNFK
HHCCEEHHEEHHCCCCCEEEEEEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHHCCCC
GTTEFVVTSLEMLKTQGIVKDSDIVVIISGNPNRATNKGTEFMEINTVEDAIKERNL
CCHHHHHHHHHHHHHCCCEECCCEEEEEECCCCCCCCCCCEEEEECCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9403685 [H]