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The map label for this gene is eno [H]

Identifier: 187918207

GI number: 187918207

Start: 348454

End: 349755

Strand: Direct

Name: eno [H]

Synonym: BH0337

Alternate gene names: 187918207

Gene position: 348454-349755 (Clockwise)

Preceding gene: 187918205

Following gene: 187918217

Centisome position: 37.78

GC content: 37.02

Gene sequence:

>1302_bases
ATGGGCTTTCGTATTTATGAAATAAAAGCTAGGCAAATAATTGATTCTAGAGGAAATCCAACTGTCGAAGCAGATGTAAT
ACTTGAAGACGGTTCTCTAGGTAGGGCAGCTGTTCCATCAGGAGCATCAACAGGAATAAATGAAGCTGTTGAACTGAGAG
ATGGTGATAAATCTGTTTACATGGGAAAAGGAGTACTTAAAGCAGTTGAAAATATAATAAATATCATATCTCCAGAGCTT
GAAGGAATGAGTGCCTTAAACCAAGTCGAAATCGACAGAAAAATGCTTGCACTTGACGGAACTCCTAACAAATCAAAACT
TGGAGCTAATGCTATTCTTGCAGTTTCAATGGCCACAGCTAGAGCAGCAGCTGAATATCTTGGACTTAAAGTTTATCAAT
ATCTTGGTGCCTACAAAGCCAACATCTTACCTACACCTATGTGTAACATTATAAATGGCGGCGCCCACTCTGATAACTGT
GTTGATTTTCAAGAATTTATGATAATGCCAATTGGAGCTAAAACTTTTAATGACGCAATAAGAATGTCTGCTGAGGTTTT
TCACACACTCAAGGGTATTTTAAGCAAAAGGGGCTACGCAACATCTGTTGGGGATGAAGGCGGATTTGCACCAAATCTAA
AGTCAAACGAAGAAGCTTGTGAAGTTATTATGGAAGCCATCAAAAATGCAGGATATACACCTGGAACAGATATCGCAATT
GCCCTTGATCCAGCAACATCTGAATTATATGATCCAGAAACAAAAAAATATGTACTTAGATGGTCAACAAAAGAAGAACT
CACATCCGAAGAAATGGTTGAATATTGGGCAAAATGGGTAGAAAAATATCCTATTATATCAATTGAAGATGGCATGGCTG
AAGAAGACTGGGACGGATGGAAAAAGCTTACAGATAAGATTGGGGATAAAGTTCAACTTGTTGGTGATGATTTATTTGTA
ACAAACACATCATTCCTTAAAAAAGGAATTGAAATGAAAGTTGCAAATGCAATTCTCATTAAAGTAAATCAAATTGGAAC
ATTAACTGAAACTTTTGAAGCTGTAGAGATGGCCAAAAAAGCAGGATATACTGCGATAGTTTCACATCGCTCAGGAGAAA
CAGAAGATACAACGATTGCCGATCTTGTTGTTGCGCTTGGAACAGGACAAATCAAAACAGGCTCCTTATCAAGGACAGAC
AGAATAGCTAAGTATAATCAACTCTTAAGAATAGAAGAAGAACTAGGCAGTATTGCTGAATACCATGGAAGAGATGTCTT
TTATTCCATTAAAAAACAATAA

Upstream 100 bases:

>100_bases
ATAAACAATTCTTTTAGCAAAATACTACACATTTGTCTTATTTGTTATTATACTGAAAATATATACTAAAGTAAATATAT
TAACCTATAAGGAGCGGAAA

Downstream 100 bases:

>100_bases
TATAAATTAAAGCCCTCATAAGAGGGCTTTAATTTATACAAACAAAATAACAAAAACAAATACTTATCTTTTAGAAAATT
GAAAACTCTTCCTAGCTTTT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase [H]

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MGFRIYEIKARQIIDSRGNPTVEADVILEDGSLGRAAVPSGASTGINEAVELRDGDKSVYMGKGVLKAVENIINIISPEL
EGMSALNQVEIDRKMLALDGTPNKSKLGANAILAVSMATARAAAEYLGLKVYQYLGAYKANILPTPMCNIINGGAHSDNC
VDFQEFMIMPIGAKTFNDAIRMSAEVFHTLKGILSKRGYATSVGDEGGFAPNLKSNEEACEVIMEAIKNAGYTPGTDIAI
ALDPATSELYDPETKKYVLRWSTKEELTSEEMVEYWAKWVEKYPIISIEDGMAEEDWDGWKKLTDKIGDKVQLVGDDLFV
TNTSFLKKGIEMKVANAILIKVNQIGTLTETFEAVEMAKKAGYTAIVSHRSGETEDTTIADLVVALGTGQIKTGSLSRTD
RIAKYNQLLRIEEELGSIAEYHGRDVFYSIKKQ

Sequences:

>Translated_433_residues
MGFRIYEIKARQIIDSRGNPTVEADVILEDGSLGRAAVPSGASTGINEAVELRDGDKSVYMGKGVLKAVENIINIISPEL
EGMSALNQVEIDRKMLALDGTPNKSKLGANAILAVSMATARAAAEYLGLKVYQYLGAYKANILPTPMCNIINGGAHSDNC
VDFQEFMIMPIGAKTFNDAIRMSAEVFHTLKGILSKRGYATSVGDEGGFAPNLKSNEEACEVIMEAIKNAGYTPGTDIAI
ALDPATSELYDPETKKYVLRWSTKEELTSEEMVEYWAKWVEKYPIISIEDGMAEEDWDGWKKLTDKIGDKVQLVGDDLFV
TNTSFLKKGIEMKVANAILIKVNQIGTLTETFEAVEMAKKAGYTAIVSHRSGETEDTTIADLVVALGTGQIKTGSLSRTD
RIAKYNQLLRIEEELGSIAEYHGRDVFYSIKKQ
>Mature_432_residues
GFRIYEIKARQIIDSRGNPTVEADVILEDGSLGRAAVPSGASTGINEAVELRDGDKSVYMGKGVLKAVENIINIISPELE
GMSALNQVEIDRKMLALDGTPNKSKLGANAILAVSMATARAAAEYLGLKVYQYLGAYKANILPTPMCNIINGGAHSDNCV
DFQEFMIMPIGAKTFNDAIRMSAEVFHTLKGILSKRGYATSVGDEGGFAPNLKSNEEACEVIMEAIKNAGYTPGTDIAIA
LDPATSELYDPETKKYVLRWSTKEELTSEEMVEYWAKWVEKYPIISIEDGMAEEDWDGWKKLTDKIGDKVQLVGDDLFVT
NTSFLKKGIEMKVANAILIKVNQIGTLTETFEAVEMAKKAGYTAIVSHRSGETEDTTIADLVVALGTGQIKTGSLSRTDR
IAKYNQLLRIEEELGSIAEYHGRDVFYSIKKQ

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis [H]

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family [H]

Homologues:

Organism=Homo sapiens, GI5803011, Length=432, Percent_Identity=51.6203703703704, Blast_Score=420, Evalue=1e-117,
Organism=Homo sapiens, GI301897477, Length=431, Percent_Identity=50.3480278422274, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI301897469, Length=431, Percent_Identity=50.3480278422274, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI4503571, Length=432, Percent_Identity=49.537037037037, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI301897479, Length=429, Percent_Identity=45.2214452214452, Blast_Score=349, Evalue=3e-96,
Organism=Homo sapiens, GI169201331, Length=353, Percent_Identity=27.1954674220963, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI169201757, Length=353, Percent_Identity=27.1954674220963, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI239744207, Length=353, Percent_Identity=27.1954674220963, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI310129182, Length=225, Percent_Identity=24.8888888888889, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI310110045, Length=225, Percent_Identity=24.8888888888889, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI310120572, Length=225, Percent_Identity=24.8888888888889, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1789141, Length=428, Percent_Identity=59.3457943925234, Blast_Score=485, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI71995829, Length=434, Percent_Identity=51.1520737327189, Blast_Score=419, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17536383, Length=434, Percent_Identity=51.1520737327189, Blast_Score=419, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI32563855, Length=193, Percent_Identity=47.6683937823834, Blast_Score=181, Evalue=5e-46,
Organism=Saccharomyces cerevisiae, GI6324974, Length=435, Percent_Identity=48.9655172413793, Blast_Score=388, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6324969, Length=435, Percent_Identity=48.9655172413793, Blast_Score=388, Evalue=1e-109,
Organism=Saccharomyces cerevisiae, GI6323985, Length=435, Percent_Identity=48.735632183908, Blast_Score=387, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6321693, Length=441, Percent_Identity=47.3922902494331, Blast_Score=378, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6321968, Length=441, Percent_Identity=48.0725623582766, Blast_Score=360, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24580918, Length=432, Percent_Identity=49.537037037037, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580916, Length=432, Percent_Identity=49.537037037037, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580920, Length=432, Percent_Identity=49.537037037037, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI24580914, Length=432, Percent_Identity=49.537037037037, Blast_Score=386, Evalue=1e-107,
Organism=Drosophila melanogaster, GI281360527, Length=432, Percent_Identity=49.537037037037, Blast_Score=385, Evalue=1e-107,
Organism=Drosophila melanogaster, GI17137654, Length=432, Percent_Identity=49.537037037037, Blast_Score=385, Evalue=1e-107,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811 [H]

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N [H]

EC number: =4.2.1.11 [H]

Molecular weight: Translated: 47355; Mature: 47223

Theoretical pI: Translated: 4.70; Mature: 4.70

Prosite motif: PS00164 ENOLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFRIYEIKARQIIDSRGNPTVEADVILEDGSLGRAAVPSGASTGINEAVELRDGDKSVY
CCEEEEEEHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHEEECCCCCEEE
MGKGVLKAVENIINIISPELEGMSALNQVEIDRKMLALDGTPNKSKLGANAILAVSMATA
ECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEECCCCCHHHCCCCCEEEEHHHHH
RAAAEYLGLKVYQYLGAYKANILPTPMCNIINGGAHSDNCVDFQEFMIMPIGAKTFNDAI
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCHHHHEEEECCCHHHHHHH
RMSAEVFHTLKGILSKRGYATSVGDEGGFAPNLKSNEEACEVIMEAIKNAGYTPGTDIAI
HHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEE
ALDPATSELYDPETKKYVLRWSTKEELTSEEMVEYWAKWVEKYPIISIEDGMAEEDWDGW
EECCCCHHHCCCCCCEEEEEECCHHHCCHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHH
KKLTDKIGDKVQLVGDDLFVTNTSFLKKGIEMKVANAILIKVNQIGTLTETFEAVEMAKK
HHHHHHCCCEEEEECCCEEEECHHHHHCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHHH
AGYTAIVSHRSGETEDTTIADLVVALGTGQIKTGSLSRTDRIAKYNQLLRIEEELGSIAE
CCCEEEEECCCCCCCCHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
YHGRDVFYSIKKQ
HCCCEEEEEECCC
>Mature Secondary Structure 
GFRIYEIKARQIIDSRGNPTVEADVILEDGSLGRAAVPSGASTGINEAVELRDGDKSVY
CEEEEEEHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCHHEEECCCCCEEE
MGKGVLKAVENIINIISPELEGMSALNQVEIDRKMLALDGTPNKSKLGANAILAVSMATA
ECHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEEECCCCCHHHCCCCCEEEEHHHHH
RAAAEYLGLKVYQYLGAYKANILPTPMCNIINGGAHSDNCVDFQEFMIMPIGAKTFNDAI
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCHHHHEEEECCCHHHHHHH
RMSAEVFHTLKGILSKRGYATSVGDEGGFAPNLKSNEEACEVIMEAIKNAGYTPGTDIAI
HHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEE
ALDPATSELYDPETKKYVLRWSTKEELTSEEMVEYWAKWVEKYPIISIEDGMAEEDWDGW
EECCCCHHHCCCCCCEEEEEECCHHHCCHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHH
KKLTDKIGDKVQLVGDDLFVTNTSFLKKGIEMKVANAILIKVNQIGTLTETFEAVEMAKK
HHHHHHCCCEEEEECCCEEEECHHHHHCCCCEEEEEEEEEEEECCCCHHHHHHHHHHHHH
AGYTAIVSHRSGETEDTTIADLVVALGTGQIKTGSLSRTDRIAKYNQLLRIEEELGSIAE
CCCEEEEECCCCCCCCHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHH
YHGRDVFYSIKKQ
HCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA