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The map label for this gene is cadA [H]

Identifier: 187736325

GI number: 187736325

Start: 2234017

End: 2235894

Strand: Direct

Name: cadA [H]

Synonym: Amuc_1839

Alternate gene names: 187736325

Gene position: 2234017-2235894 (Clockwise)

Preceding gene: 187736324

Following gene: 187736326

Centisome position: 83.86

GC content: 59.0

Gene sequence:

>1878_bases
ATGCTGGACCGATTGGAAAAATTGCTGGAATGGGGAGGGACAAGGCGGAAAGTCGCACTGTTGTCCGTTTCCGGATTAGC
TCTGTTGATGAGCATGCTTGGCATGGAACCTTTGCCCTTTGAAATTTCATGGATAGCCATTGTGCTGTGCGGCGTTCCCA
TTGTGCTGGAAGCCGTCCTTGGCCTGGCGACGGCCTGGGATATTAAGGCGGATGTTCTTGTTTCCGTCGCTCTGGTTGCC
TCCGTCATCATTGGGGAGGACTTCGCTGCGGCGGAAATTGCATTTATCATGCAGCTTGGGGAACTCCTTGAGGAGCTGAC
CGTAGCCCGGGCCCGGAAGGGTATTGAAAGGCTGGTCCGTTTGACCCCGGCGACCGCCCGGAAAGTGGAAGGAACCAGGG
AGGAGGTTATTCCGGCGGAAGAGGTTCGCATCGGGGATATTCTCCGCGTATTGCCCGGAGAGACGATTCCGGCGGACGGC
GTTATTCTTTCCGGGCACACTTCCGTCAACCAGTCCGTAATGACGGGGGAACCTCTTCCGATAGACAAGGAAACCGGGGA
TGAGGTTTCCAGCGGGACGGTCAACCAGTTCGGAACCTTTGACATGAAGGCGGCCAGGACCGGGAAGGACAGTTCCATCC
AGCGCATGGTGCGCCTCGTCCAATCCGCAGATGCGGGGAAGGCTAAAATAGTGAGGCTGGCAGACCGCTGGGCCACCTGG
ATCGTTGCCATCGCCCTGGTTTCCGCAGGGGGGGCCTGGATGGCCACGGGCGAACTGATCCGCGCCGTTACCATTCTGGT
GGTATTCTGTCCCTGCGCGCTTGTGCTGGCAACTCCCACGGCGGTCATGGCCGCTATCGGGAACGCCACCAGGCATGGCT
TCCTGGTGCGGGAAGGGGATGCCCTGGAACGCCTGTCCTGCGTGTCCCACCTCACTTTCGACAAGACGGGCACGCTTACC
TGCGGTGCTCCGCGCGTGGCGGCCGTGCGCAGTTTCCTGCCGGAGCTCCCGGAACAGGAACTTTACAGATATGCCGCCTG
TGCGGAACTGCGTTCTGAACATCCTCTGGGCAAGGCGATTGTCCGCTGTTACCGGAAAGATTCAGTGCAGAAACTTCCCC
AACCGGAACAATTCCGCATGATTCCCGGCAGGGGAGTACGGGCTGTGGCGCAGGGAAAGGAACTTCTGGCCGGCAACCTG
GAATTGTTCAGGGAAAACGGGGTGGAGCTGTCCGATGAGGCCAGACGTGCGGCGGAACGGTATCAGGAGGAGGGGTGCTC
CGTGATTTTCATAGGAATGGACCGGCAGGCCGCAGGGTTGATCGCCCTGTCGGATACCTTGCGGGAGAATGCCGTAGCTA
CCATCCGGGAAGTACGGGATGCCGGAGTGGTTCCCGTATTGCTGACCGGGGACCATGGGAATGCCGCCGCGCGTGTCGCC
GGACAACTGGGGATTGACCTGGTTTGCGCGGAATGCCTGCCGGAGGACAAATTCAACTGGATTGACGCGTGCCAGAAAGA
GCGGAACCGTGTGTGCATGGTTGGGGACGGAATCAATGATGCCCTTGCCTTGAAAACCTCTCATGCGGGAATTGCCATGG
GCGGCATAGGAAGTGACATTGCCGTGGATGCCGCAGATATTGTCCTGGTCAATGACGATATCCGGGAACTGCCCCATCTG
CTCCGTCTGTCAAGGCGCATGATGGGGACAATCAAATGCAATCTGACATTCTCCATGGCCCTGAACTTCATCGCCATCAT
CCTGGCGGCGGGCGGCATCCTGAATCCCGTGGCTGGCGCGCTGGTTCACAATGCGGGCTCCGTCGTCGTGATCGCCAACT
CCGTTTTTCTGCTGAAATGGAGGAGGAAAAACGCGTGA

Upstream 100 bases:

>100_bases
AAGGTTCGGGAGGGGGGCTCATTTTTTTCTTGAAAAACCTATACCCCTATAGGTATATAAATGCCCGTACCCGTATGGGT
ATGAAAAGAAAAATCATCAT

Downstream 100 bases:

>100_bases
ATCCGCCTGGAGGGTAGAGAAACTTCCCCGTGTTGATTGCCGGGAGGGAACGGAGTCCGTCTTTTTGACGCTCCGGTTTC
ACCGCGCCGGGCTGGAAAAC

Product: heavy metal translocating P-type ATPase

Products: NA

Alternate protein names: Cadmium efflux ATPase [H]

Number of amino acids: Translated: 625; Mature: 625

Protein sequence:

>625_residues
MLDRLEKLLEWGGTRRKVALLSVSGLALLMSMLGMEPLPFEISWIAIVLCGVPIVLEAVLGLATAWDIKADVLVSVALVA
SVIIGEDFAAAEIAFIMQLGELLEELTVARARKGIERLVRLTPATARKVEGTREEVIPAEEVRIGDILRVLPGETIPADG
VILSGHTSVNQSVMTGEPLPIDKETGDEVSSGTVNQFGTFDMKAARTGKDSSIQRMVRLVQSADAGKAKIVRLADRWATW
IVAIALVSAGGAWMATGELIRAVTILVVFCPCALVLATPTAVMAAIGNATRHGFLVREGDALERLSCVSHLTFDKTGTLT
CGAPRVAAVRSFLPELPEQELYRYAACAELRSEHPLGKAIVRCYRKDSVQKLPQPEQFRMIPGRGVRAVAQGKELLAGNL
ELFRENGVELSDEARRAAERYQEEGCSVIFIGMDRQAAGLIALSDTLRENAVATIREVRDAGVVPVLLTGDHGNAAARVA
GQLGIDLVCAECLPEDKFNWIDACQKERNRVCMVGDGINDALALKTSHAGIAMGGIGSDIAVDAADIVLVNDDIRELPHL
LRLSRRMMGTIKCNLTFSMALNFIAIILAAGGILNPVAGALVHNAGSVVVIANSVFLLKWRRKNA

Sequences:

>Translated_625_residues
MLDRLEKLLEWGGTRRKVALLSVSGLALLMSMLGMEPLPFEISWIAIVLCGVPIVLEAVLGLATAWDIKADVLVSVALVA
SVIIGEDFAAAEIAFIMQLGELLEELTVARARKGIERLVRLTPATARKVEGTREEVIPAEEVRIGDILRVLPGETIPADG
VILSGHTSVNQSVMTGEPLPIDKETGDEVSSGTVNQFGTFDMKAARTGKDSSIQRMVRLVQSADAGKAKIVRLADRWATW
IVAIALVSAGGAWMATGELIRAVTILVVFCPCALVLATPTAVMAAIGNATRHGFLVREGDALERLSCVSHLTFDKTGTLT
CGAPRVAAVRSFLPELPEQELYRYAACAELRSEHPLGKAIVRCYRKDSVQKLPQPEQFRMIPGRGVRAVAQGKELLAGNL
ELFRENGVELSDEARRAAERYQEEGCSVIFIGMDRQAAGLIALSDTLRENAVATIREVRDAGVVPVLLTGDHGNAAARVA
GQLGIDLVCAECLPEDKFNWIDACQKERNRVCMVGDGINDALALKTSHAGIAMGGIGSDIAVDAADIVLVNDDIRELPHL
LRLSRRMMGTIKCNLTFSMALNFIAIILAAGGILNPVAGALVHNAGSVVVIANSVFLLKWRRKNA
>Mature_625_residues
MLDRLEKLLEWGGTRRKVALLSVSGLALLMSMLGMEPLPFEISWIAIVLCGVPIVLEAVLGLATAWDIKADVLVSVALVA
SVIIGEDFAAAEIAFIMQLGELLEELTVARARKGIERLVRLTPATARKVEGTREEVIPAEEVRIGDILRVLPGETIPADG
VILSGHTSVNQSVMTGEPLPIDKETGDEVSSGTVNQFGTFDMKAARTGKDSSIQRMVRLVQSADAGKAKIVRLADRWATW
IVAIALVSAGGAWMATGELIRAVTILVVFCPCALVLATPTAVMAAIGNATRHGFLVREGDALERLSCVSHLTFDKTGTLT
CGAPRVAAVRSFLPELPEQELYRYAACAELRSEHPLGKAIVRCYRKDSVQKLPQPEQFRMIPGRGVRAVAQGKELLAGNL
ELFRENGVELSDEARRAAERYQEEGCSVIFIGMDRQAAGLIALSDTLRENAVATIREVRDAGVVPVLLTGDHGNAAARVA
GQLGIDLVCAECLPEDKFNWIDACQKERNRVCMVGDGINDALALKTSHAGIAMGGIGSDIAVDAADIVLVNDDIRELPHL
LRLSRRMMGTIKCNLTFSMALNFIAIILAAGGILNPVAGALVHNAGSVVVIANSVFLLKWRRKNA

Specific function: This electroneutral antiporter ejects one cadmium ion while accumulating two protons by an energy-dependent efflux mechanism [H]

COG id: COG2217

COG function: function code P; Cation transport ATPase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 HMA domains [H]

Homologues:

Organism=Homo sapiens, GI55743071, Length=579, Percent_Identity=31.433506044905, Blast_Score=283, Evalue=2e-76,
Organism=Homo sapiens, GI55743073, Length=497, Percent_Identity=32.5955734406439, Blast_Score=266, Evalue=4e-71,
Organism=Homo sapiens, GI115529486, Length=596, Percent_Identity=29.8657718120805, Blast_Score=261, Evalue=2e-69,
Organism=Homo sapiens, GI48762685, Length=177, Percent_Identity=28.2485875706215, Blast_Score=73, Evalue=9e-13,
Organism=Homo sapiens, GI48762689, Length=177, Percent_Identity=28.2485875706215, Blast_Score=73, Evalue=1e-12,
Organism=Homo sapiens, GI48762687, Length=177, Percent_Identity=28.2485875706215, Blast_Score=73, Evalue=1e-12,
Organism=Homo sapiens, GI48762691, Length=177, Percent_Identity=28.2485875706215, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI297374799, Length=197, Percent_Identity=24.8730964467005, Blast_Score=69, Evalue=2e-11,
Organism=Homo sapiens, GI83700225, Length=197, Percent_Identity=24.8730964467005, Blast_Score=69, Evalue=2e-11,
Organism=Homo sapiens, GI118498343, Length=95, Percent_Identity=35.7894736842105, Blast_Score=67, Evalue=6e-11,
Organism=Escherichia coli, GI1786691, Length=553, Percent_Identity=38.5171790235081, Blast_Score=317, Evalue=1e-87,
Organism=Escherichia coli, GI1789879, Length=597, Percent_Identity=33.8358458961474, Blast_Score=279, Evalue=4e-76,
Organism=Escherichia coli, GI1786914, Length=510, Percent_Identity=28.2352941176471, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI2367363, Length=609, Percent_Identity=22.6600985221675, Blast_Score=83, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17556548, Length=583, Percent_Identity=32.2469982847341, Blast_Score=237, Evalue=1e-62,
Organism=Caenorhabditis elegans, GI17557486, Length=247, Percent_Identity=25.5060728744939, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71997262, Length=202, Percent_Identity=27.7227722772277, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71997275, Length=202, Percent_Identity=27.7227722772277, Blast_Score=70, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI25152707, Length=456, Percent_Identity=22.3684210526316, Blast_Score=69, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71999438, Length=456, Percent_Identity=22.3684210526316, Blast_Score=69, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71999440, Length=456, Percent_Identity=22.3684210526316, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71997269, Length=181, Percent_Identity=28.1767955801105, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320475, Length=541, Percent_Identity=30.1293900184843, Blast_Score=229, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6319772, Length=609, Percent_Identity=28.2430213464696, Blast_Score=197, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6321430, Length=521, Percent_Identity=24.9520153550864, Blast_Score=110, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6325221, Length=523, Percent_Identity=25.6214149139579, Blast_Score=105, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6321271, Length=186, Percent_Identity=31.1827956989247, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6320244, Length=225, Percent_Identity=26.6666666666667, Blast_Score=78, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320243, Length=225, Percent_Identity=26.6666666666667, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6320245, Length=225, Percent_Identity=26.6666666666667, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6321432, Length=183, Percent_Identity=27.8688524590164, Blast_Score=68, Evalue=4e-12,
Organism=Drosophila melanogaster, GI221329854, Length=375, Percent_Identity=32.8, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI281366617, Length=237, Percent_Identity=27.0042194092827, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24668704, Length=237, Percent_Identity=27.0042194092827, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24668708, Length=237, Percent_Identity=27.0042194092827, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI161085803, Length=237, Percent_Identity=27.0042194092827, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24668696, Length=237, Percent_Identity=27.0042194092827, Blast_Score=75, Evalue=2e-13,
Organism=Drosophila melanogaster, GI281366676, Length=200, Percent_Identity=25, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008250
- InterPro:   IPR001366
- InterPro:   IPR006404
- InterPro:   IPR006416
- InterPro:   IPR001757
- InterPro:   IPR018303
- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR017969
- InterPro:   IPR006121 [H]

Pfam domain/function: PF00122 E1-E2_ATPase; PF00403 HMA; PF00702 Hydrolase [H]

EC number: =3.6.3.3 [H]

Molecular weight: Translated: 67064; Mature: 67064

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: PS00154 ATPASE_E1_E2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDRLEKLLEWGGTRRKVALLSVSGLALLMSMLGMEPLPFEISWIAIVLCGVPIVLEAVL
CHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHH
GLATAWDIKADVLVSVALVASVIIGEDFAAAEIAFIMQLGELLEELTVARARKGIERLVR
HHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTPATARKVEGTREEVIPAEEVRIGDILRVLPGETIPADGVILSGHTSVNQSVMTGEPLP
CCCHHHHHCCCCHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCC
IDKETGDEVSSGTVNQFGTFDMKAARTGKDSSIQRMVRLVQSADAGKAKIVRLADRWATW
CCCCCCCCCCCCCCHHCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IVAIALVSAGGAWMATGELIRAVTILVVFCPCALVLATPTAVMAAIGNATRHGFLVREGD
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCEEEECCC
ALERLSCVSHLTFDKTGTLTCGAPRVAAVRSFLPELPEQELYRYAACAELRSEHPLGKAI
HHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHH
VRCYRKDSVQKLPQPEQFRMIPGRGVRAVAQGKELLAGNLELFRENGVELSDEARRAAER
HHHHHHHHHHHCCCCCCEEECCCCCCHHHHCCHHHHHCCHHHHHHCCCCCCHHHHHHHHH
YQEEGCSVIFIGMDRQAAGLIALSDTLRENAVATIREVRDAGVVPVLLTGDHGNAAARVA
HHHCCCEEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHH
GQLGIDLVCAECLPEDKFNWIDACQKERNRVCMVGDGINDALALKTSHAGIAMGGIGSDI
HHHCHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCEEECCCCCCC
AVDAADIVLVNDDIRELPHLLRLSRRMMGTIKCNLTFSMALNFIAIILAAGGILNPVAGA
EECCEEEEEECCHHHHHHHHHHHHHHHHEEEEEEEHHHHHHHHHHHHHHCCCHHHHHHHH
LVHNAGSVVVIANSVFLLKWRRKNA
HHHCCCCEEEEECCEEEEEEECCCC
>Mature Secondary Structure
MLDRLEKLLEWGGTRRKVALLSVSGLALLMSMLGMEPLPFEISWIAIVLCGVPIVLEAVL
CHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHCCCCCCCHHHHHHHHHHCHHHHHHHHH
GLATAWDIKADVLVSVALVASVIIGEDFAAAEIAFIMQLGELLEELTVARARKGIERLVR
HHHHHCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTPATARKVEGTREEVIPAEEVRIGDILRVLPGETIPADGVILSGHTSVNQSVMTGEPLP
CCCHHHHHCCCCHHHCCCHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCCCCCC
IDKETGDEVSSGTVNQFGTFDMKAARTGKDSSIQRMVRLVQSADAGKAKIVRLADRWATW
CCCCCCCCCCCCCCHHCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
IVAIALVSAGGAWMATGELIRAVTILVVFCPCALVLATPTAVMAAIGNATRHGFLVREGD
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCEEEECCC
ALERLSCVSHLTFDKTGTLTCGAPRVAAVRSFLPELPEQELYRYAACAELRSEHPLGKAI
HHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHH
VRCYRKDSVQKLPQPEQFRMIPGRGVRAVAQGKELLAGNLELFRENGVELSDEARRAAER
HHHHHHHHHHHCCCCCCEEECCCCCCHHHHCCHHHHHCCHHHHHHCCCCCCHHHHHHHHH
YQEEGCSVIFIGMDRQAAGLIALSDTLRENAVATIREVRDAGVVPVLLTGDHGNAAARVA
HHHCCCEEEEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHH
GQLGIDLVCAECLPEDKFNWIDACQKERNRVCMVGDGINDALALKTSHAGIAMGGIGSDI
HHHCHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEECCCCCCEEEEECCCCEEECCCCCCC
AVDAADIVLVNDDIRELPHLLRLSRRMMGTIKCNLTFSMALNFIAIILAAGGILNPVAGA
EECCEEEEEECCHHHHHHHHHHHHHHHHEEEEEEEHHHHHHHHHHHHHHCCCHHHHHHHH
LVHNAGSVVVIANSVFLLKWRRKNA
HHHCCCCEEEEECCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11302791 [H]