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The map label for this gene is rfaE [C]

Identifier: 187736080

GI number: 187736080

Start: 1914118

End: 1915209

Strand: Reverse

Name: rfaE [C]

Synonym: Amuc_1591

Alternate gene names: 187736080

Gene position: 1915209-1914118 (Counterclockwise)

Preceding gene: 187736081

Following gene: 187736079

Centisome position: 71.89

GC content: 57.78

Gene sequence:

>1092_bases
ATGAAAAAAGTTTTCGTCTCCGGCTGCTATGATATCGTCCACGCTGGCCATATCCAGTTCTTTGAAGAAGCCCGCGCTCT
GGGCGACTATCTAATCGTCTCTTTCGCTTCGGAACCCGTGCTGTGGCACCACAAGCAGCGCAAGCCGTCCATTCCGGACG
AACACAAAAAAGTTCTGCTGGAAAGCCTGCGCATGGTTGACAAGGTTATCCTGGGTACCGGCATGAAAAAGGGCCTGGAT
TTTGAAGAAGAGTTTCTTCAGGAAAAACCGGACATCCTGGCTGTGACGGAAGACGACCTTTACAGCGATATCAAGAAGGA
GCTGTGCGCCCGCGTCGGAGCCAATTACGTGGTGCTCCCCAAGACGCCCCCCAAATTCACTCCCGTCTCCACCACCATGC
TGGTGAACCGCATCAAGGCTCCGTCCGCCGTACCGCTTCGCGTGGACTTTGCCGGGGGATGGCTGGACGTGCCCCGCTAC
GCCAGAAAAGGCTCCTATGTGGTCAACTGCGCCATCACCCCCATGGTTTCCCTCTGCGAATGGCCGTATGAAAAACGCTC
CGGGCTGGGAGGCAGCGGCGCGTGGGCCATGCTGGAAGGGCGCGATCCGGTCGCGTCCGAACTCGCTCTGGGCGTCGGTT
GGCAGGATCCTGCCGTCATTGCGGAAACGGGGTTGTGCGTATGGCGCTCCGGCAGTTCTCCGGTGCTGGATGTCAAAGGC
ACGGGTGACTTCCTGGAAGGAAGAATGGCCATTCTATATACGGGTGAAGAACACGACACCCCAAAGATGGCGGATGAACA
ACGGGACTACGTACGCATCTCCCAATCCTCCCTCATTGCCCGCACCGGAGTGCTGGAACGCAACATCAATACACTGGCGG
CAGGCGTAGCCCTGTACTACAGCGTGCAGCTTGACGAAGGAATGCGCCCCCTGCCGGATATTCCCAATGCCCTTGCAAAA
AAATATCTGGGAGGCGGCTACGGGGGATACGCCCTGTACCTTTTCCCCTGCCGGGATGATCGCGACCAGGCTGTCAAGGA
CAACCCGGCAATGAAACGGGTGGAGCCGTATTGCCGCCAGCTGTTCAAGTAA

Upstream 100 bases:

>100_bases
CTGTAGTCCGCGCCGTTTTTTAACGCATTTTCATCTCGCTCTTGCATCCGGCACGGCAATAGGGCATACTGTTGCAGACT
AATTATCTATCATTACACGC

Downstream 100 bases:

>100_bases
ATTCCCCCCCTTTCATGTCCCTGATGCGGAACAGCCTGGTTGCCTCCGGCGCCATCTTCGCCTGCCGCCTGACAGGCATG
GCCAGGGAAATTGTGTACAC

Product: cytidyltransferase-related domain protein

Products: diphosphate; CDPglycerol

Alternate protein names: NA

Number of amino acids: Translated: 363; Mature: 363

Protein sequence:

>363_residues
MKKVFVSGCYDIVHAGHIQFFEEARALGDYLIVSFASEPVLWHHKQRKPSIPDEHKKVLLESLRMVDKVILGTGMKKGLD
FEEEFLQEKPDILAVTEDDLYSDIKKELCARVGANYVVLPKTPPKFTPVSTTMLVNRIKAPSAVPLRVDFAGGWLDVPRY
ARKGSYVVNCAITPMVSLCEWPYEKRSGLGGSGAWAMLEGRDPVASELALGVGWQDPAVIAETGLCVWRSGSSPVLDVKG
TGDFLEGRMAILYTGEEHDTPKMADEQRDYVRISQSSLIARTGVLERNINTLAAGVALYYSVQLDEGMRPLPDIPNALAK
KYLGGGYGGYALYLFPCRDDRDQAVKDNPAMKRVEPYCRQLFK

Sequences:

>Translated_363_residues
MKKVFVSGCYDIVHAGHIQFFEEARALGDYLIVSFASEPVLWHHKQRKPSIPDEHKKVLLESLRMVDKVILGTGMKKGLD
FEEEFLQEKPDILAVTEDDLYSDIKKELCARVGANYVVLPKTPPKFTPVSTTMLVNRIKAPSAVPLRVDFAGGWLDVPRY
ARKGSYVVNCAITPMVSLCEWPYEKRSGLGGSGAWAMLEGRDPVASELALGVGWQDPAVIAETGLCVWRSGSSPVLDVKG
TGDFLEGRMAILYTGEEHDTPKMADEQRDYVRISQSSLIARTGVLERNINTLAAGVALYYSVQLDEGMRPLPDIPNALAK
KYLGGGYGGYALYLFPCRDDRDQAVKDNPAMKRVEPYCRQLFK
>Mature_363_residues
MKKVFVSGCYDIVHAGHIQFFEEARALGDYLIVSFASEPVLWHHKQRKPSIPDEHKKVLLESLRMVDKVILGTGMKKGLD
FEEEFLQEKPDILAVTEDDLYSDIKKELCARVGANYVVLPKTPPKFTPVSTTMLVNRIKAPSAVPLRVDFAGGWLDVPRY
ARKGSYVVNCAITPMVSLCEWPYEKRSGLGGSGAWAMLEGRDPVASELALGVGWQDPAVIAETGLCVWRSGSSPVLDVKG
TGDFLEGRMAILYTGEEHDTPKMADEQRDYVRISQSSLIARTGVLERNINTLAAGVALYYSVQLDEGMRPLPDIPNALAK
KYLGGGYGGYALYLFPCRDDRDQAVKDNPAMKRVEPYCRQLFK

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0615

COG function: function code MI; Cytidylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004821
- InterPro:   IPR004820
- InterPro:   IPR014729 [H]

Pfam domain/function: PF01467 CTP_transf_2 [H]

EC number: 2.7.7.39

Molecular weight: Translated: 40325; Mature: 40325

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVFVSGCYDIVHAGHIQFFEEARALGDYLIVSFASEPVLWHHKQRKPSIPDEHKKVLL
CCEEEEEHHHHHHHCCHHHHHHHHHHHHHEEEEEECCCCEEECCCCCCCCCCHHHHHHHH
ESLRMVDKVILGTGMKKGLDFEEEFLQEKPDILAVTEDDLYSDIKKELCARVGANYVVLP
HHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHCCCEEEEC
KTPPKFTPVSTTMLVNRIKAPSAVPLRVDFAGGWLDVPRYARKGSYVVNCAITPMVSLCE
CCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHCCCCEEEEEEHHHHHHHHC
WPYEKRSGLGGSGAWAMLEGRDPVASELALGVGWQDPAVIAETGLCVWRSGSSPVLDVKG
CCCHHHCCCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCEEECCEEEEECCCCCEEEECC
TGDFLEGRMAILYTGEEHDTPKMADEQRDYVRISQSSLIARTGVLERNINTLAAGVALYY
CCCHHCCCEEEEEECCCCCCCCCCCCCHHHEEECHHHHHHHHHHHHHCHHHHHHHEHEEE
SVQLDEGMRPLPDIPNALAKKYLGGGYGGYALYLFPCRDDRDQAVKDNPAMKRVEPYCRQ
EEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHH
LFK
HCC
>Mature Secondary Structure
MKKVFVSGCYDIVHAGHIQFFEEARALGDYLIVSFASEPVLWHHKQRKPSIPDEHKKVLL
CCEEEEEHHHHHHHCCHHHHHHHHHHHHHEEEEEECCCCEEECCCCCCCCCCHHHHHHHH
ESLRMVDKVILGTGMKKGLDFEEEFLQEKPDILAVTEDDLYSDIKKELCARVGANYVVLP
HHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHCCCEEEEC
KTPPKFTPVSTTMLVNRIKAPSAVPLRVDFAGGWLDVPRYARKGSYVVNCAITPMVSLCE
CCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCCCHHHCCCCEEEEEEHHHHHHHHC
WPYEKRSGLGGSGAWAMLEGRDPVASELALGVGWQDPAVIAETGLCVWRSGSSPVLDVKG
CCCHHHCCCCCCCCEEEEECCCCHHHHHHHCCCCCCCCCEEECCEEEEECCCCCEEEECC
TGDFLEGRMAILYTGEEHDTPKMADEQRDYVRISQSSLIARTGVLERNINTLAAGVALYY
CCCHHCCCEEEEEECCCCCCCCCCCCCHHHEEECHHHHHHHHHHHHHCHHHHHHHEHEEE
SVQLDEGMRPLPDIPNALAKKYLGGGYGGYALYLFPCRDDRDQAVKDNPAMKRVEPYCRQ
EEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHH
LFK
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: CTP; sn-glycerol 3-phosphate

Specific reaction: CTP + sn-glycerol 3-phosphate = diphosphate + CDP-glycerol

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]