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The map label for this gene is citZ [H]

Identifier: 187736062

GI number: 187736062

Start: 1895915

End: 1897057

Strand: Direct

Name: citZ [H]

Synonym: Amuc_1572

Alternate gene names: 187736062

Gene position: 1895915-1897057 (Clockwise)

Preceding gene: 187736061

Following gene: 187736064

Centisome position: 71.17

GC content: 57.22

Gene sequence:

>1143_bases
ATGAGCAATGACACTTTTTCCGAGCGCCTCGCATATCCCAAAGGTTTAAAAGGAATCATCGCCAATGAATCCGCTTTGAG
TGACGTGCGCGGCGAGGAAGGGCGTCTGTTGTATCTGGGCTACGATATCGATGATCTGGTGGAGATGTGCTGTTTTGAAG
AAGTGGTTTATCTGCTTCTCAACAAGCGTCTTCCCAACCGTGAGGAATTGGAGGGCATCAAGAAACGCCTCCGTTCCGAC
CGCGACCTGCCCCAGCCCATTCTGGATTTCTTCAAAACGGCCACCAAGTCCGCCCGGCCCATGTCCGCCCTCCGGACGGC
TGTTTCCATGCTCGGCATGTATGACGACCGGACGAAGGATGCCGGGCAGTTGAAGGAAATAGGCCTCAGCCTCATAGCCA
AGGTTCCCGTGATGGTGGCGTATTACTACCGTTTGTGCCAGGGTCTGGAGCTTCCCCCCGTTCGGGAGGACCTGAGCGAG
GCGGAACATTTCCTGTGGTTGCTGAAGGGTGAGGAACCGGATCAGTATGAAGCCCATATTCTGGAGGTAGCCTACATTCT
GCACGCAGACCACGGCATGAATGCCTCCACCTTTGCGGCGCGCGTCTGCATCGCCACGCTTTCGGACATGTATTCCGCCA
TCACCGCGGCCATCGGCACGCTGAAGGGGCCGCTTCATGGGGGCGCCAATGAGGGCGTGATTGAAATGCTCAAGGAAATA
GGCACGGAGGACCGGGTGGACTCCTACATTGAGGACAAGCTGGCGCGGAAGGAAAAAATCATGGGCATGGGCCACCGCGT
GTACCGCGTGCTGGATCCCCGCGCCCCGCATTTGCGACGCATGGCCATCCGCCTTTCCTCCCGAATCGGTGAACCCAAGT
GGATCCGCATGTCGGAACGCATCGCGAAACTTGTCCGGGAGCGCAAGGGCTTGAACGCCAATGTGGATTTTTATTCCGCC
ACGGTTTATTACAGCATCGGGATCCCCACCAGGCTGTTTACCGCCATTTTCTCCATTGCCCGCTGTAGCGGCTGGGTGGC
CCAGGTTCTGGAACAAATGGAGGATAACACGCTGTACAGGCCGCTGACTCTTTACACAGGGCCCAAGGACCGCATTCCGG
TTCCTACGATCGACATGCGGTGA

Upstream 100 bases:

>100_bases
ACGTCCCGCATGGGCCGTGAAGGCGTAATTAAGGTTTGACTTTTTGGAAGATTGTTAGACTTTTAACCGGTAATATTCAC
CACCAGTAGCATTTTTTAAC

Downstream 100 bases:

>100_bases
CAGGTTTTAATCAGGAAAGGCGCCGGAAGCAATATCCGGCGCCTTTCCTGCATCTGCCTTCCGCTTTTCTTTTTCGTGAA
AATATCCTTTCTATCCGCCG

Product: 2-methylcitrate synthase/citrate synthase II

Products: NA

Alternate protein names: Citrate synthase II [H]

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MSNDTFSERLAYPKGLKGIIANESALSDVRGEEGRLLYLGYDIDDLVEMCCFEEVVYLLLNKRLPNREELEGIKKRLRSD
RDLPQPILDFFKTATKSARPMSALRTAVSMLGMYDDRTKDAGQLKEIGLSLIAKVPVMVAYYYRLCQGLELPPVREDLSE
AEHFLWLLKGEEPDQYEAHILEVAYILHADHGMNASTFAARVCIATLSDMYSAITAAIGTLKGPLHGGANEGVIEMLKEI
GTEDRVDSYIEDKLARKEKIMGMGHRVYRVLDPRAPHLRRMAIRLSSRIGEPKWIRMSERIAKLVRERKGLNANVDFYSA
TVYYSIGIPTRLFTAIFSIARCSGWVAQVLEQMEDNTLYRPLTLYTGPKDRIPVPTIDMR

Sequences:

>Translated_380_residues
MSNDTFSERLAYPKGLKGIIANESALSDVRGEEGRLLYLGYDIDDLVEMCCFEEVVYLLLNKRLPNREELEGIKKRLRSD
RDLPQPILDFFKTATKSARPMSALRTAVSMLGMYDDRTKDAGQLKEIGLSLIAKVPVMVAYYYRLCQGLELPPVREDLSE
AEHFLWLLKGEEPDQYEAHILEVAYILHADHGMNASTFAARVCIATLSDMYSAITAAIGTLKGPLHGGANEGVIEMLKEI
GTEDRVDSYIEDKLARKEKIMGMGHRVYRVLDPRAPHLRRMAIRLSSRIGEPKWIRMSERIAKLVRERKGLNANVDFYSA
TVYYSIGIPTRLFTAIFSIARCSGWVAQVLEQMEDNTLYRPLTLYTGPKDRIPVPTIDMR
>Mature_379_residues
SNDTFSERLAYPKGLKGIIANESALSDVRGEEGRLLYLGYDIDDLVEMCCFEEVVYLLLNKRLPNREELEGIKKRLRSDR
DLPQPILDFFKTATKSARPMSALRTAVSMLGMYDDRTKDAGQLKEIGLSLIAKVPVMVAYYYRLCQGLELPPVREDLSEA
EHFLWLLKGEEPDQYEAHILEVAYILHADHGMNASTFAARVCIATLSDMYSAITAAIGTLKGPLHGGANEGVIEMLKEIG
TEDRVDSYIEDKLARKEKIMGMGHRVYRVLDPRAPHLRRMAIRLSSRIGEPKWIRMSERIAKLVRERKGLNANVDFYSAT
VYYSIGIPTRLFTAIFSIARCSGWVAQVLEQMEDNTLYRPLTLYTGPKDRIPVPTIDMR

Specific function: Might regulate the synthesis and function of enzymes involved in later enzymatic steps of Krebs cycle. Loss in activity results in sporulation defect [H]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=375, Percent_Identity=25.0666666666667, Blast_Score=98, Evalue=1e-20,
Organism=Escherichia coli, GI1786527, Length=389, Percent_Identity=37.7892030848329, Blast_Score=258, Evalue=6e-70,
Organism=Escherichia coli, GI1786939, Length=379, Percent_Identity=31.1345646437995, Blast_Score=181, Evalue=8e-47,
Organism=Caenorhabditis elegans, GI17555174, Length=374, Percent_Identity=24.5989304812834, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6319850, Length=322, Percent_Identity=25.4658385093168, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6324328, Length=323, Percent_Identity=24.1486068111455, Blast_Score=87, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6325257, Length=361, Percent_Identity=23.8227146814404, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24640126, Length=328, Percent_Identity=25.9146341463415, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI24640124, Length=328, Percent_Identity=25.9146341463415, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21356863, Length=329, Percent_Identity=26.4437689969605, Blast_Score=81, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011278
- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 43089; Mature: 42957

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNDTFSERLAYPKGLKGIIANESALSDVRGEEGRLLYLGYDIDDLVEMCCFEEVVYLLL
CCCCCHHHHHCCCCCCCHHHCCHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHH
NKRLPNREELEGIKKRLRSDRDLPQPILDFFKTATKSARPMSALRTAVSMLGMYDDRTKD
HCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCC
AGQLKEIGLSLIAKVPVMVAYYYRLCQGLELPPVREDLSEAEHFLWLLKGEEPDQYEAHI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEEEEEECCCCCHHHHHH
LEVAYILHADHGMNASTFAARVCIATLSDMYSAITAAIGTLKGPLHGGANEGVIEMLKEI
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHC
GTEDRVDSYIEDKLARKEKIMGMGHRVYRVLDPRAPHLRRMAIRLSSRIGEPKWIRMSER
CCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
IAKLVRERKGLNANVDFYSATVYYSIGIPTRLFTAIFSIARCSGWVAQVLEQMEDNTLYR
HHHHHHHHCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEC
PLTLYTGPKDRIPVPTIDMR
CEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
SNDTFSERLAYPKGLKGIIANESALSDVRGEEGRLLYLGYDIDDLVEMCCFEEVVYLLL
CCCCHHHHHCCCCCCCHHHCCHHHHHHCCCCCCCEEEECCCHHHHHHHHHHHHHHHHHH
NKRLPNREELEGIKKRLRSDRDLPQPILDFFKTATKSARPMSALRTAVSMLGMYDDRTKD
HCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCC
AGQLKEIGLSLIAKVPVMVAYYYRLCQGLELPPVREDLSEAEHFLWLLKGEEPDQYEAHI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHEEEEEECCCCCHHHHHH
LEVAYILHADHGMNASTFAARVCIATLSDMYSAITAAIGTLKGPLHGGANEGVIEMLKEI
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHC
GTEDRVDSYIEDKLARKEKIMGMGHRVYRVLDPRAPHLRRMAIRLSSRIGEPKWIRMSER
CCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHH
IAKLVRERKGLNANVDFYSATVYYSIGIPTRLFTAIFSIARCSGWVAQVLEQMEDNTLYR
HHHHHHHHCCCCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEC
PLTLYTGPKDRIPVPTIDMR
CEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8045898; 9387221; 9384377 [H]