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The map label for this gene is ogt [H]

Identifier: 187736057

GI number: 187736057

Start: 1892289

End: 1892816

Strand: Direct

Name: ogt [H]

Synonym: Amuc_1567

Alternate gene names: 187736057

Gene position: 1892289-1892816 (Clockwise)

Preceding gene: 187736050

Following gene: 187736061

Centisome position: 71.03

GC content: 51.7

Gene sequence:

>528_bases
ATGGAGGCAATTAAAATAATCCGTTATCTGTCGCCCGTTGGAGAAATGATGATTGGCTCATATGGCGGCAATCTTTGCAT
TTGCGACTGGGCAAAGGAAAAACGCCGCGGCACGATAGACCGCCGCATTTGCCGGCATCTCAACGCTGAATATGAAGAAG
GAACTTCCGAGGTTATTGAACAGGCGGTAAGCCAGCTCCGTGAATATTTCCAGGGGGAACGCAGAGACTTTGATATACCC
ATTGTATTTACCGGATCGGAATTTCAAAACTCAGTCTGGCATGAACTGATGAAAGTTCCTTACGGTTCCACCATTTCTTA
TGCAGAGCTGGCGCGCCGCATTCATCATCCGAGGGCTGTCCGGGCTGTAGCGTCCGCTAATGCCACCAACCCCATCTCCA
TTTTCGTGCCCTGCCACCGCGTTATTGGCAGCAATCAGAAACTGACAGGTTATGGCGGGGGGCTTGATGCGAAACGGGAA
TTGCTTGCCCTGGAGGCGCGGGTAAGCGGAAGTGTGCTGGCCTTATGA

Upstream 100 bases:

>100_bases
ACATTGTGCTTCGGGACATGAACAGGCAGGCATGGAGCAATGTTTCCCAGCCGTACAGGGGATGGATGAAAGAGCGTTAT
GCAATAACAAGGACAAGAAG

Downstream 100 bases:

>100_bases
CCCGTGATGTGAGGAAGACAGGCAAGGACGTGTCAGGTAAGGCTGTTTTGGGAGGCTGCTCTTCCGGGGCAGCCCGGCAG
GGGGGGCGTTAAGAAGCTTC

Product: methylated-DNA/protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 175; Mature: 175

Protein sequence:

>175_residues
MEAIKIIRYLSPVGEMMIGSYGGNLCICDWAKEKRRGTIDRRICRHLNAEYEEGTSEVIEQAVSQLREYFQGERRDFDIP
IVFTGSEFQNSVWHELMKVPYGSTISYAELARRIHHPRAVRAVASANATNPISIFVPCHRVIGSNQKLTGYGGGLDAKRE
LLALEARVSGSVLAL

Sequences:

>Translated_175_residues
MEAIKIIRYLSPVGEMMIGSYGGNLCICDWAKEKRRGTIDRRICRHLNAEYEEGTSEVIEQAVSQLREYFQGERRDFDIP
IVFTGSEFQNSVWHELMKVPYGSTISYAELARRIHHPRAVRAVASANATNPISIFVPCHRVIGSNQKLTGYGGGLDAKRE
LLALEARVSGSVLAL
>Mature_175_residues
MEAIKIIRYLSPVGEMMIGSYGGNLCICDWAKEKRRGTIDRRICRHLNAEYEEGTSEVIEQAVSQLREYFQGERRDFDIP
IVFTGSEFQNSVWHELMKVPYGSTISYAELARRIHHPRAVRAVASANATNPISIFVPCHRVIGSNQKLTGYGGGLDAKRE
LLALEARVSGSVLAL

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=119, Percent_Identity=37.8151260504202, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1787596, Length=104, Percent_Identity=47.1153846153846, Blast_Score=99, Evalue=1e-22,
Organism=Escherichia coli, GI1788542, Length=88, Percent_Identity=46.5909090909091, Blast_Score=87, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI115533068, Length=80, Percent_Identity=50, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI115533070, Length=80, Percent_Identity=50, Blast_Score=78, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320001, Length=95, Percent_Identity=43.1578947368421, Blast_Score=89, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19599; Mature: 19599

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAIKIIRYLSPVGEMMIGSYGGNLCICDWAKEKRRGTIDRRICRHLNAEYEEGTSEVIE
CHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHCCCHHHHHHHHCCCCCCCCHHHHHH
QAVSQLREYFQGERRDFDIPIVFTGSEFQNSVWHELMKVPYGSTISYAELARRIHHPRAV
HHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHH
RAVASANATNPISIFVPCHRVIGSNQKLTGYGGGLDAKRELLALEARVSGSVLAL
HHHHHCCCCCCEEEEEEHHHHHCCCCEEECCCCCCCHHHHHHHHHHHCCCCEECC
>Mature Secondary Structure
MEAIKIIRYLSPVGEMMIGSYGGNLCICDWAKEKRRGTIDRRICRHLNAEYEEGTSEVIE
CHHHHHHHHHHHHHHHHHHCCCCCEEEECCCHHHHCCCHHHHHHHHCCCCCCCCHHHHHH
QAVSQLREYFQGERRDFDIPIVFTGSEFQNSVWHELMKVPYGSTISYAELARRIHHPRAV
HHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCHHHH
RAVASANATNPISIFVPCHRVIGSNQKLTGYGGGLDAKRELLALEARVSGSVLAL
HHHHHCCCCCCEEEEEEHHHHHCCCCEEECCCCCCCHHHHHHHHHHHCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA