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The map label for this gene is rpoA [H]

Identifier: 187735995

GI number: 187735995

Start: 1797639

End: 1798763

Strand: Direct

Name: rpoA [H]

Synonym: Amuc_1505

Alternate gene names: 187735995

Gene position: 1797639-1798763 (Clockwise)

Preceding gene: 187735992

Following gene: 187735996

Centisome position: 67.48

GC content: 55.73

Gene sequence:

>1125_bases
ATGTCGGAAAACGAAACCACTACTGCAGAAGAAACCGCCGTTACCACGCTGGCCCGTTTTGAGGTTCCCAGCCGTCTGGA
AAAAATTGAAGATCCGAATGATGCGAATCATTTGACCTTTGTGGCGGAACCGTTTGAAAACGGTTACGGCCATACGCTTG
GCAACTCCCTGCGCCGCGTTCTTCTGGGTTCTCTTGAAGGCGCCGCCATTACCTCCGTCCGCATCGCCGGTGCGCAGCAT
GAATTTTCCTCCCTGCCCGGTGTGGTGGAAGATGTGACGGAAATCGTGCTGAACCTGAAAAAGGTCAAATTCAAGCATAA
CGGCAAGGAACCGCGCCTGCTCTCTCTGCGCGTGCATAAGCAGGGCGTTGTGACTGCTGCCGATATTGCCGACGACACCG
TTTACCAGGTGGTCAATCCCGATCAAATCATTTGCACTCTGGATCAGGACACCATGTTCGAATGCGAATTCCAGGTTCGC
GTGGGCCGCGGTTTTGCCACCGGCGATGAAAACAAGGTGCCGGATATGCCCATCGGCGTGATTCCGATCGACTCCATTTT
CTCTCCGGTTACCCGCGTGAAGTATTCTGTGCAGAATACCCGTGTGGGGCAGATGACTGACTACGACAAGCTGATCCTTG
AAGTCTGGACGGACGGCCGCATCGCCCCCGCAGACGCCATGCTCCAGGCTTCCGCCATTCTGCGCCATCACCTGGACGTA
TTCGTCAACTATGACGACAAGCAGGTCAACTTTGAGGAAGCTCCTTCCGCCGTCCAGGACGAGGAAACGGCCCGCCTGCG
CAAGTTGCTCAACATGAGCGTCAACGAAATCGAGCTTTCCGTGCGTGCCGCCAACTGCCTCAACAATGCCAATATCACGA
CCGTTGGCCAGCTTGCCATGAAGAGCGAAAGCGAAATGCTCAAGTACCGCAATTTCGGCAAAAAATCCCTCAACGAAATC
AAGGACAAATTGCTGGAACTGGGTCTTTCCCTGGGCGTTCAGGTGGATCCATCCCTGCTTACCGGTCCCGTGCCGCAGGC
CAAGCCGCGCCTGGGTCTGGAAGAGGAAAGCCCCGTGGGCCTGGCAGACCTGATTGCCGCCAACATCGAAGACGACGACG
ACTAA

Upstream 100 bases:

>100_bases
TGCTCCCCGCACGGGCGTTCCCACAACATGCCGTTGGTCCTTCAGGCGCATTGGCCGCCATGTCCGTGTTGAGGGGAAAC
CCGGTACATTACATACGATT

Downstream 100 bases:

>100_bases
ATCTCACAAAACCAACACACATAGAAAAATATCATCATGAGACACGGTGTAAAAACCTCCAAGCTTCAGCGCAATGCTTC
TCACCGCAGGGCTCTGCTCG

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha [H]

Number of amino acids: Translated: 374; Mature: 373

Protein sequence:

>374_residues
MSENETTTAEETAVTTLARFEVPSRLEKIEDPNDANHLTFVAEPFENGYGHTLGNSLRRVLLGSLEGAAITSVRIAGAQH
EFSSLPGVVEDVTEIVLNLKKVKFKHNGKEPRLLSLRVHKQGVVTAADIADDTVYQVVNPDQIICTLDQDTMFECEFQVR
VGRGFATGDENKVPDMPIGVIPIDSIFSPVTRVKYSVQNTRVGQMTDYDKLILEVWTDGRIAPADAMLQASAILRHHLDV
FVNYDDKQVNFEEAPSAVQDEETARLRKLLNMSVNEIELSVRAANCLNNANITTVGQLAMKSESEMLKYRNFGKKSLNEI
KDKLLELGLSLGVQVDPSLLTGPVPQAKPRLGLEEESPVGLADLIAANIEDDDD

Sequences:

>Translated_374_residues
MSENETTTAEETAVTTLARFEVPSRLEKIEDPNDANHLTFVAEPFENGYGHTLGNSLRRVLLGSLEGAAITSVRIAGAQH
EFSSLPGVVEDVTEIVLNLKKVKFKHNGKEPRLLSLRVHKQGVVTAADIADDTVYQVVNPDQIICTLDQDTMFECEFQVR
VGRGFATGDENKVPDMPIGVIPIDSIFSPVTRVKYSVQNTRVGQMTDYDKLILEVWTDGRIAPADAMLQASAILRHHLDV
FVNYDDKQVNFEEAPSAVQDEETARLRKLLNMSVNEIELSVRAANCLNNANITTVGQLAMKSESEMLKYRNFGKKSLNEI
KDKLLELGLSLGVQVDPSLLTGPVPQAKPRLGLEEESPVGLADLIAANIEDDDD
>Mature_373_residues
SENETTTAEETAVTTLARFEVPSRLEKIEDPNDANHLTFVAEPFENGYGHTLGNSLRRVLLGSLEGAAITSVRIAGAQHE
FSSLPGVVEDVTEIVLNLKKVKFKHNGKEPRLLSLRVHKQGVVTAADIADDTVYQVVNPDQIICTLDQDTMFECEFQVRV
GRGFATGDENKVPDMPIGVIPIDSIFSPVTRVKYSVQNTRVGQMTDYDKLILEVWTDGRIAPADAMLQASAILRHHLDVF
VNYDDKQVNFEEAPSAVQDEETARLRKLLNMSVNEIELSVRAANCLNNANITTVGQLAMKSESEMLKYRNFGKKSLNEIK
DKLLELGLSLGVQVDPSLLTGPVPQAKPRLGLEEESPVGLADLIAANIEDDDD

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family [H]

Homologues:

Organism=Escherichia coli, GI1789690, Length=306, Percent_Identity=41.1764705882353, Blast_Score=226, Evalue=2e-60,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- ProDom:   PD001179 [H]

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 41198; Mature: 41067

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSENETTTAEETAVTTLARFEVPSRLEKIEDPNDANHLTFVAEPFENGYGHTLGNSLRRV
CCCCCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHH
LLGSLEGAAITSVRIAGAQHEFSSLPGVVEDVTEIVLNLKKVKFKHNGKEPRLLSLRVHK
HHHCCCCCEEEEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHEECCCCCCCEEEEEEEC
QGVVTAADIADDTVYQVVNPDQIICTLDQDTMFECEFQVRVGRGFATGDENKVPDMPIGV
CCCEEEECCCCCCEEEECCCCEEEEEECCCCEEEEEEEEEECCCEECCCCCCCCCCCEEE
IPIDSIFSPVTRVKYSVQNTRVGQMTDYDKLILEVWTDGRIAPADAMLQASAILRHHLDV
EEHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCEE
FVNYDDKQVNFEEAPSAVQDEETARLRKLLNMSVNEIELSVRAANCLNNANITTVGQLAM
EEECCCCCCCHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCCCEEHHHHHH
KSESEMLKYRNFGKKSLNEIKDKLLELGLSLGVQVDPSLLTGPVPQAKPRLGLEEESPVG
HCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEECCHHHCCCCCCCCCCCCCCCCCCCC
LADLIAANIEDDDD
HHHHHHCCCCCCCC
>Mature Secondary Structure 
SENETTTAEETAVTTLARFEVPSRLEKIEDPNDANHLTFVAEPFENGYGHTLGNSLRRV
CCCCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCEEEEEECCCCCCCCHHHHHHHHHH
LLGSLEGAAITSVRIAGAQHEFSSLPGVVEDVTEIVLNLKKVKFKHNGKEPRLLSLRVHK
HHHCCCCCEEEEEEEECCCHHHHHCCCHHHHHHHHHHHHHHHHEECCCCCCCEEEEEEEC
QGVVTAADIADDTVYQVVNPDQIICTLDQDTMFECEFQVRVGRGFATGDENKVPDMPIGV
CCCEEEECCCCCCEEEECCCCEEEEEECCCCEEEEEEEEEECCCEECCCCCCCCCCCEEE
IPIDSIFSPVTRVKYSVQNTRVGQMTDYDKLILEVWTDGRIAPADAMLQASAILRHHLDV
EEHHHHHHHHHHHEEECCCCCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCEE
FVNYDDKQVNFEEAPSAVQDEETARLRKLLNMSVNEIELSVRAANCLNNANITTVGQLAM
EEECCCCCCCHHHCCCCCCCHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCCCEEHHHHHH
KSESEMLKYRNFGKKSLNEIKDKLLELGLSLGVQVDPSLLTGPVPQAKPRLGLEEESPVG
HCHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCEECCHHHCCCCCCCCCCCCCCCCCCCC
LADLIAANIEDDDD
HHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA