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The map label for this gene is rsmE [H]

Identifier: 187735952

GI number: 187735952

Start: 1751967

End: 1752704

Strand: Direct

Name: rsmE [H]

Synonym: Amuc_1461

Alternate gene names: 187735952

Gene position: 1751967-1752704 (Clockwise)

Preceding gene: 187735951

Following gene: 187735953

Centisome position: 65.76

GC content: 64.23

Gene sequence:

>738_bases
ATGGCCCGCTTTTTCCTCCCGCCTTCCGAATGGGCTGCTCCCGCCTGGGAGCTGCGAGGGGATGAAGCGCACCATGCCGC
CAAGGTTCTGCGCCTGAAGCAGGGTGACTCCTGCATCGTGTTTGACGGTTGCGGCCGTGCGGCCCACGCTGTGGTGGCGG
AACCTCCGCGCAGCTCCGGCGTGCTGCTGGTTCCGGGGGAGGAATGTCCTCCTTCTCCCCCCGTGGCCCATTTGACGCTG
TGCCAGGCTGTTCCCAAGGGGGCCAACATGGATCTCATTATCCAAAAGTCTGTGGAACTGGGTGTTTCTGCCATTGTTCC
GCTCGTGACGGACCGCACGATCGTGCGCCTGAATGCCCGGGAGGCTGAGGCCAAAAGGCAGAAATGGCAGCGCATCGCCT
TGGAGGCCTGCAAGCAGTGCGGCCAGAACACGCTGCCGGAAGTGGCGCTTCCCGTTCCCTTTGCGGAATGGCTGCGCGGA
GGAATTCCGGAAGGACTGAATATTATCGCCTCTCTGGCGCCCGGAGTGCGACCGGTCAGGGAAGTGCTGGAGACCGCCCG
TTCCCGTTCCGTCCGTCACGCTTCTCTGCTGGTGGGGCCGGAGGGGGATTTTACGGACCGGGAGACGGCCATGGCGTTGG
AAGCGGGGTTTGTTCCCGTCACGCTTGGCCCTATCGTTCTCCGGGTGGAGACGGCCGCCTTTTTCGGCCTGGCCGCCATG
CGGTACGCCCTGGACTGA

Upstream 100 bases:

>100_bases
GCCCTGGATGAAAATCGGAACCAGCCGGCTTGCGTGGAGTTTGCCGACAAGGCGGCGCGTTACCTGAAGGGCAAATAAGG
TCCGTTTCCCTTTTTCAGAC

Downstream 100 bases:

>100_bases
GTTTTTTTCATACGCATTCCCATGTTTAAAAATATCATTTTCGACTGGTCCGGCACCCTGGTGGACGACCTGGCCCTGAC
GCTGGACGCCTCCAACTACG

Product: hypothetical protein

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MARFFLPPSEWAAPAWELRGDEAHHAAKVLRLKQGDSCIVFDGCGRAAHAVVAEPPRSSGVLLVPGEECPPSPPVAHLTL
CQAVPKGANMDLIIQKSVELGVSAIVPLVTDRTIVRLNAREAEAKRQKWQRIALEACKQCGQNTLPEVALPVPFAEWLRG
GIPEGLNIIASLAPGVRPVREVLETARSRSVRHASLLVGPEGDFTDRETAMALEAGFVPVTLGPIVLRVETAAFFGLAAM
RYALD

Sequences:

>Translated_245_residues
MARFFLPPSEWAAPAWELRGDEAHHAAKVLRLKQGDSCIVFDGCGRAAHAVVAEPPRSSGVLLVPGEECPPSPPVAHLTL
CQAVPKGANMDLIIQKSVELGVSAIVPLVTDRTIVRLNAREAEAKRQKWQRIALEACKQCGQNTLPEVALPVPFAEWLRG
GIPEGLNIIASLAPGVRPVREVLETARSRSVRHASLLVGPEGDFTDRETAMALEAGFVPVTLGPIVLRVETAAFFGLAAM
RYALD
>Mature_244_residues
ARFFLPPSEWAAPAWELRGDEAHHAAKVLRLKQGDSCIVFDGCGRAAHAVVAEPPRSSGVLLVPGEECPPSPPVAHLTLC
QAVPKGANMDLIIQKSVELGVSAIVPLVTDRTIVRLNAREAEAKRQKWQRIALEACKQCGQNTLPEVALPVPFAEWLRGG
IPEGLNIIASLAPGVRPVREVLETARSRSVRHASLLVGPEGDFTDRETAMALEAGFVPVTLGPIVLRVETAAFFGLAAMR
YALD

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=225, Percent_Identity=33.7777777777778, Blast_Score=123, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 26329; Mature: 26198

Theoretical pI: Translated: 7.01; Mature: 7.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARFFLPPSEWAAPAWELRGDEAHHAAKVLRLKQGDSCIVFDGCGRAAHAVVAEPPRSSG
CCCCCCCCHHHCCCCCCCCCCHHHHHHHHHHEECCCCEEEECCCCCHHEEEEECCCCCCC
VLLVPGEECPPSPPVAHLTLCQAVPKGANMDLIIQKSVELGVSAIVPLVTDRTIVRLNAR
EEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCHHHCHHHHHHHHCCCEEEEECCH
EAEAKRQKWQRIALEACKQCGQNTLPEVALPVPFAEWLRGGIPEGLNIIASLAPGVRPVR
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHCCCCCHHHHHHHHHCCCHHHHH
EVLETARSRSVRHASLLVGPEGDFTDRETAMALEAGFVPVTLGPIVLRVETAAFFGLAAM
HHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHCCCEEEEECCEEEEEHHHHHHHHHHH
RYALD
HHHCC
>Mature Secondary Structure 
ARFFLPPSEWAAPAWELRGDEAHHAAKVLRLKQGDSCIVFDGCGRAAHAVVAEPPRSSG
CCCCCCCHHHCCCCCCCCCCHHHHHHHHHHEECCCCEEEECCCCCHHEEEEECCCCCCC
VLLVPGEECPPSPPVAHLTLCQAVPKGANMDLIIQKSVELGVSAIVPLVTDRTIVRLNAR
EEEECCCCCCCCCCHHHHHHHHHCCCCCCEEEEEECCHHHCHHHHHHHHCCCEEEEECCH
EAEAKRQKWQRIALEACKQCGQNTLPEVALPVPFAEWLRGGIPEGLNIIASLAPGVRPVR
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCHHHHHCCCCCHHHHHHHHHCCCHHHHH
EVLETARSRSVRHASLLVGPEGDFTDRETAMALEAGFVPVTLGPIVLRVETAAFFGLAAM
HHHHHHHHCCCEEEEEEECCCCCCCCCHHHHHHHCCCEEEEECCEEEEEHHHHHHHHHHH
RYALD
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]