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The map label for this gene is rsuA [H]

Identifier: 187735933

GI number: 187735933

Start: 1731771

End: 1732463

Strand: Reverse

Name: rsuA [H]

Synonym: Amuc_1442

Alternate gene names: 187735933

Gene position: 1732463-1731771 (Counterclockwise)

Preceding gene: 187735936

Following gene: 187735932

Centisome position: 65.03

GC content: 59.88

Gene sequence:

>693_bases
ATGAGACTGGATGCCCTTTTATCCCGCTACGGATATTGTTCCAGGAGGGAAGCGCCGGGTTGGATCAAACGCCACTCCAT
TACATTCAAGGGAAAACCATGCTCCTCCCCCACGGACAAGGTGCAGGCGGAAGGCATCCTGCTGGACGGAGAACCTGTGG
AATTCCCGGACGGCCTGTACGCCGCCCTTTACAAACCTGAAGGGTACACCTGCAGCCATGACGACGGGGAAGGGAATTTG
ATTTACGACCTACTGCCCTTCCAGTGGCGGCACAGGAACCCTGCGGTTTCTTCCGTAGGACGGCTGGACAAGGAAACTTC
CGGCCTGCTTCTCCTCACGGACGACGGAAAATTCATCCACCGCATGACCTCCCCCCGCCATCACGTCGCCAAAGTTTACG
AAGCCGTCACGGAGGAGGACATTCCGACAGAAGCCGTGGAGTTGTTCGCCTCCGGAACCCTCACGCTGAACGGGGAAACC
CGCCCCTGCGCCCCCGCCTTGCTGGAAATCCGGGAACCGCGACGCGCCCTGCTGACGCTTACGGAAGGAAAATACCACCA
GGTTCGCCGCATGCTGGCTGCCGTAGGCGCTCCCGTCTTATCTCTGTGCCGTGTTTCCATCGGCTCCCTGCATCTGGACA
GCCTGAACCTGAAACCGGGGGAATGGGCGCCCATCCGTCCGGATGCGCTGTAA

Upstream 100 bases:

>100_bases
GCCCCACCAGGGCGGCCCCGCATGTAATAGTTTAATGCTATCAGCCGGGGAGGCATACTTCAACCTTGTTTTTTGTTCCC
GTTAGGAAAAAATATGCCGC

Downstream 100 bases:

>100_bases
ACCTCCCATCCGCCATGTGGATTCCCATTTCCGAAGCACCAATTGCCTGTTTTCCGCACTTTGAGGTGGCGGCGGAATCC
GGGGACTGGATTGTGGTGGA

Product: pseudouridine synthase

Products: pseudouridine 5'-phosphate; H2O [C]

Alternate protein names: 16S pseudouridine 516 synthase; 16S pseudouridylate 516 synthase; rRNA pseudouridylate synthase A; rRNA-uridine isomerase A [H]

Number of amino acids: Translated: 230; Mature: 230

Protein sequence:

>230_residues
MRLDALLSRYGYCSRREAPGWIKRHSITFKGKPCSSPTDKVQAEGILLDGEPVEFPDGLYAALYKPEGYTCSHDDGEGNL
IYDLLPFQWRHRNPAVSSVGRLDKETSGLLLLTDDGKFIHRMTSPRHHVAKVYEAVTEEDIPTEAVELFASGTLTLNGET
RPCAPALLEIREPRRALLTLTEGKYHQVRRMLAAVGAPVLSLCRVSIGSLHLDSLNLKPGEWAPIRPDAL

Sequences:

>Translated_230_residues
MRLDALLSRYGYCSRREAPGWIKRHSITFKGKPCSSPTDKVQAEGILLDGEPVEFPDGLYAALYKPEGYTCSHDDGEGNL
IYDLLPFQWRHRNPAVSSVGRLDKETSGLLLLTDDGKFIHRMTSPRHHVAKVYEAVTEEDIPTEAVELFASGTLTLNGET
RPCAPALLEIREPRRALLTLTEGKYHQVRRMLAAVGAPVLSLCRVSIGSLHLDSLNLKPGEWAPIRPDAL
>Mature_230_residues
MRLDALLSRYGYCSRREAPGWIKRHSITFKGKPCSSPTDKVQAEGILLDGEPVEFPDGLYAALYKPEGYTCSHDDGEGNL
IYDLLPFQWRHRNPAVSSVGRLDKETSGLLLLTDDGKFIHRMTSPRHHVAKVYEAVTEEDIPTEAVELFASGTLTLNGET
RPCAPALLEIREPRRALLTLTEGKYHQVRRMLAAVGAPVLSLCRVSIGSLHLDSLNLKPGEWAPIRPDAL

Specific function: Responsible for synthesis of pseudouridine from uracil- 516 in 16S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788510, Length=229, Percent_Identity=38.8646288209607, Blast_Score=145, Evalue=2e-36,
Organism=Escherichia coli, GI1790453, Length=235, Percent_Identity=30.6382978723404, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI87081838, Length=169, Percent_Identity=35.5029585798817, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1787524, Length=236, Percent_Identity=29.6610169491525, Blast_Score=81, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: 4.2.1.70 [C]

Molecular weight: Translated: 25544; Mature: 25544

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLDALLSRYGYCSRREAPGWIKRHSITFKGKPCSSPTDKVQAEGILLDGEPVEFPDGLY
CCHHHHHHHCCCCCCCCCCCCEEECEEEECCCCCCCCHHHHEECEEEECCCCCCCCCCEE
AALYKPEGYTCSHDDGEGNLIYDLLPFQWRHRNPAVSSVGRLDKETSGLLLLTDDGKFIH
EEEECCCCCEECCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCEEH
RMTSPRHHVAKVYEAVTEEDIPTEAVELFASGTLTLNGETRPCAPALLEIREPRRALLTL
HHCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEECCCCCCCCHHHHHHHCCCEEEEEE
TEGKYHQVRRMLAAVGAPVLSLCRVSIGSLHLDSLNLKPGEWAPIRPDAL
CCCHHHHHHHHHHHHCHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRLDALLSRYGYCSRREAPGWIKRHSITFKGKPCSSPTDKVQAEGILLDGEPVEFPDGLY
CCHHHHHHHCCCCCCCCCCCCEEECEEEECCCCCCCCHHHHEECEEEECCCCCCCCCCEE
AALYKPEGYTCSHDDGEGNLIYDLLPFQWRHRNPAVSSVGRLDKETSGLLLLTDDGKFIH
EEEECCCCCEECCCCCCCCEEEEECCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCEEH
RMTSPRHHVAKVYEAVTEEDIPTEAVELFASGTLTLNGETRPCAPALLEIREPRRALLTL
HHCCCHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEECCCCCCCCHHHHHHHCCCEEEEEE
TEGKYHQVRRMLAAVGAPVLSLCRVSIGSLHLDSLNLKPGEWAPIRPDAL
CCCHHHHHHHHHHHHCHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose phosphate [C]

Specific reaction: uracil + D-ribose phosphate = pseudouridine 5'-phosphate + H2O [C]

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]