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The map label for this gene is tilS [H]

Identifier: 187735920

GI number: 187735920

Start: 1715438

End: 1716442

Strand: Reverse

Name: tilS [H]

Synonym: Amuc_1429

Alternate gene names: 187735920

Gene position: 1716442-1715438 (Counterclockwise)

Preceding gene: 187735921

Following gene: 187735918

Centisome position: 64.43

GC content: 59.5

Gene sequence:

>1005_bases
ATGTTGACACCGGACCATTTTGACAGCCAGTCCGCGGAACTCCTCCGCTCCGGCCGCCCCGTCCTTGCCGGAATCAGCGG
GGGGAGGGACTCCATGGCTTTGCTTTCCCTGCTGTCCGGCATGCCGGGATGCAATCTGGTTGCATGCCATGTAAATCACC
AGCTGCGTCCGGAAGCGGAGGAAGAGGAAATGTTTGTGCTCCGTTACGCCGCCTCCCTGGGTATTCCCTGTGTCCGGGAA
CGCGTCAATGTCCGCAGTATGGCCCATACCCGCGGAATAGCCATTGAAGAAGCGGCCAGGGAAGCCAGGCAGGCCTCGTT
TCTGAAATGGGCCGCCGGTTACCCGGGGGCTCTCGTGGCTCTGGCCCACCACCGGAACGACCAGCAGGAAACGGCCCTTC
TTCACCTCTGCCGCGGTGCCTCCGGCATTCACGGTATGTTCCCCGTCTCCACCTGGGCCAACGGATTGACCGTCGTACGC
CCCATGCTGGATTTTTCCCGGGAGGATATCACCTCCTATCTGGAGCATCGCCATATTCCATGGAGGGAAGATTCCAGCAA
CCAGTCTGCCGAATATACCAGGAACGCCCTGCGCCATGAGATTATTCCCAAATTGAATGCGCTGTTCCAAAGGGACACCA
GCCTTCCCTTTTCACGTGCCTGCCACCTGGAAAATCAAATCCGCACGGCGCTTTCCCAGGCGCTGGACTCCATGAACCTG
ACAGATCCCCAGGGGCGCCTGTTCTTGCCGAAAATCAACCTGCTTCCTGCGGAGCTGAGACAATGCGCCGTGCATGATTA
CCTGCGCCGGCAACATATTCCGGACCTGACGGAAGACGCCGTACTCCGGGTAATGGACATCCTGAATACGGACGGCCCTT
CCCGAACTTCCCTGCCCGGCGGAAAACTGGCCGTACGCAAGGAAAAGCGCCTGCTGGTCACGGACACGCCGCGCCCGGTC
AATAAAGCGGAGCAACCCCCTGCGGATACAACAGGTGGAATTTAA

Upstream 100 bases:

>100_bases
CCTTCAGCAATTTTCCAACCCCGTTTCCTTCCCGGAAAACGGGGTTTTTATTTGCAGGCTTCCTTTGATCTCTTTTTCCT
GATTCACTCGCAGCAGCACC

Downstream 100 bases:

>100_bases
TGGCTTCATTCCCCACCACCAGCGCAGTACCGAACATCCTGTCTTCATCTGTCAGCCGCGGCAGCTGGGCAATCGGGAAG
AGCAGACCGCCCAGAATAAG

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 334; Mature: 334

Protein sequence:

>334_residues
MLTPDHFDSQSAELLRSGRPVLAGISGGRDSMALLSLLSGMPGCNLVACHVNHQLRPEAEEEEMFVLRYAASLGIPCVRE
RVNVRSMAHTRGIAIEEAAREARQASFLKWAAGYPGALVALAHHRNDQQETALLHLCRGASGIHGMFPVSTWANGLTVVR
PMLDFSREDITSYLEHRHIPWREDSSNQSAEYTRNALRHEIIPKLNALFQRDTSLPFSRACHLENQIRTALSQALDSMNL
TDPQGRLFLPKINLLPAELRQCAVHDYLRRQHIPDLTEDAVLRVMDILNTDGPSRTSLPGGKLAVRKEKRLLVTDTPRPV
NKAEQPPADTTGGI

Sequences:

>Translated_334_residues
MLTPDHFDSQSAELLRSGRPVLAGISGGRDSMALLSLLSGMPGCNLVACHVNHQLRPEAEEEEMFVLRYAASLGIPCVRE
RVNVRSMAHTRGIAIEEAAREARQASFLKWAAGYPGALVALAHHRNDQQETALLHLCRGASGIHGMFPVSTWANGLTVVR
PMLDFSREDITSYLEHRHIPWREDSSNQSAEYTRNALRHEIIPKLNALFQRDTSLPFSRACHLENQIRTALSQALDSMNL
TDPQGRLFLPKINLLPAELRQCAVHDYLRRQHIPDLTEDAVLRVMDILNTDGPSRTSLPGGKLAVRKEKRLLVTDTPRPV
NKAEQPPADTTGGI
>Mature_334_residues
MLTPDHFDSQSAELLRSGRPVLAGISGGRDSMALLSLLSGMPGCNLVACHVNHQLRPEAEEEEMFVLRYAASLGIPCVRE
RVNVRSMAHTRGIAIEEAAREARQASFLKWAAGYPGALVALAHHRNDQQETALLHLCRGASGIHGMFPVSTWANGLTVVR
PMLDFSREDITSYLEHRHIPWREDSSNQSAEYTRNALRHEIIPKLNALFQRDTSLPFSRACHLENQIRTALSQALDSMNL
TDPQGRLFLPKINLLPAELRQCAVHDYLRRQHIPDLTEDAVLRVMDILNTDGPSRTSLPGGKLAVRKEKRLLVTDTPRPV
NKAEQPPADTTGGI

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=279, Percent_Identity=30.4659498207885, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 37116; Mature: 37116

Theoretical pI: Translated: 7.67; Mature: 7.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTPDHFDSQSAELLRSGRPVLAGISGGRDSMALLSLLSGMPGCNLVACHVNHQLRPEAE
CCCCCCCCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCC
EEEMFVLRYAASLGIPCVRERVNVRSMAHTRGIAIEEAAREARQASFLKWAAGYPGALVA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
LAHHRNDQQETALLHLCRGASGIHGMFPVSTWANGLTVVRPMLDFSREDITSYLEHRHIP
HHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHHCCHHHHHHHHHHCCCC
WREDSSNQSAEYTRNALRHEIIPKLNALFQRDTSLPFSRACHLENQIRTALSQALDSMNL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
TDPQGRLFLPKINLLPAELRQCAVHDYLRRQHIPDLTEDAVLRVMDILNTDGPSRTSLPG
CCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
GKLAVRKEKRLLVTDTPRPVNKAEQPPADTTGGI
CCEEEECCCEEEEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLTPDHFDSQSAELLRSGRPVLAGISGGRDSMALLSLLSGMPGCNLVACHVNHQLRPEAE
CCCCCCCCCHHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCCCCC
EEEMFVLRYAASLGIPCVRERVNVRSMAHTRGIAIEEAAREARQASFLKWAAGYPGALVA
HHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHH
LAHHRNDQQETALLHLCRGASGIHGMFPVSTWANGLTVVRPMLDFSREDITSYLEHRHIP
HHHCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHHCCHHHHHHHHHHCCCC
WREDSSNQSAEYTRNALRHEIIPKLNALFQRDTSLPFSRACHLENQIRTALSQALDSMNL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
TDPQGRLFLPKINLLPAELRQCAVHDYLRRQHIPDLTEDAVLRVMDILNTDGPSRTSLPG
CCCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCCCC
GKLAVRKEKRLLVTDTPRPVNKAEQPPADTTGGI
CCEEEECCCEEEEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA