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The map label for this gene is hupE [H]

Identifier: 187735895

GI number: 187735895

Start: 1675473

End: 1676507

Strand: Direct

Name: hupE [H]

Synonym: Amuc_1402

Alternate gene names: 187735895

Gene position: 1675473-1676507 (Clockwise)

Preceding gene: 187735894

Following gene: 187735897

Centisome position: 62.89

GC content: 61.55

Gene sequence:

>1035_bases
ATGTTTGAGTGTCCTGTTCCGGAGCCTGTTTCCGACCGTATCCAGATGGCTCATGGCGGCGGAGGCCGCTTGATGAATGA
GCTGATCCGTTCCGTGTTTCTGAGCGCCTTCGGGTGCCCTTCCGGAGGCGTGCAGAATGACGCCGCCGTATTGGAGATTC
CTCCGGGGAGGCTGGCGATGACGACGGACAGCTTTGTGGTGCAGCCTTTGGAGTTTCCCGGCGGCTCCATCGGTTCACTG
GCGGTGCACGGAACGGTGAATGATCTCGCCATGAGCGGCGCGGAGCCGTTGTATTTGACGGCGGGCTTTATTCTGGAAGA
AGGGCTTCCGCTGGAAGTTCTGGCCCGCGTGGCACAAGATATGGCTGCCGCGGCCCGTGCGGCGGGCGTCCGTATTGTGA
CGGGGGATACAAAAGTGGTGGAGCGCGGAAAGGGGGACGGCATTTACATTAATACTGCCGGGGTTGGCATCGTGCGCCAT
GGGTTGGAGATCAGCCCTTCTTCCGTTCGTCCGGGGGATTCCGTGCTGCTCAGCGGGGATTTGGGGAGGCACGGCATGAC
GATTATGAGCCTGCGCGCCGGGCTGTCTTTCGGAGACGGCCTGGAAAGTGATTCCGCTCCGTTGCATGAATCCGTGGCCG
CCGTCATTCGTGCCGGCATTCCCGTGCATTGCCTGCGTGACGTGACCCGCGGCGGGTTGACCGCCACTCTTTCGGAGATT
GCGGAATCTGCTGGCCTGACAGTGAAGCTGAATGAAATGTCCATTCCCGTGCGTGAGGATGTCAGGGCGGCGTGCGGGCT
GTTGGGGCTGGACCCTCTTCAAGTGGCCTGTGAGGGACGTTATCTGGCTGTTCTTCCACGGGAGCATGAGGAAGAGGCCC
TGAACCTGATGCGCGGCTGCGGCGTATCTGCCGGAGCCTGCGTCATAGGCCGGGTGGAGGAATTGGGGACGGCGCCCCTG
CTGATGACGGGACTTCTTGGAGTGGAGCGGGTGTTGACGATGCCTTCAGGAATGCAGCTTCCCCGCATCTGCTGA

Upstream 100 bases:

>100_bases
TTCGGATCGTCCTGCACGCCGCTGGCCCCGCTGGGGGCTCCCATGGTGTCCGGGGAAGGGGCTTGCGCCGCTTATTATCA
TTATAAAAGAAGTTGATGCC

Downstream 100 bases:

>100_bases
TTCCTGGTGCTGCATGATTGGCGTTTTGTGGCAGGGAAGCGGTGGAACCCGCTTCCCTGCCGGTCCGGTGTTTCATAGGG
GTGCCGGAGCGGCTCTGTGC

Product: hydrogenase expression/formation protein HypE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MFECPVPEPVSDRIQMAHGGGGRLMNELIRSVFLSAFGCPSGGVQNDAAVLEIPPGRLAMTTDSFVVQPLEFPGGSIGSL
AVHGTVNDLAMSGAEPLYLTAGFILEEGLPLEVLARVAQDMAAAARAAGVRIVTGDTKVVERGKGDGIYINTAGVGIVRH
GLEISPSSVRPGDSVLLSGDLGRHGMTIMSLRAGLSFGDGLESDSAPLHESVAAVIRAGIPVHCLRDVTRGGLTATLSEI
AESAGLTVKLNEMSIPVREDVRAACGLLGLDPLQVACEGRYLAVLPREHEEEALNLMRGCGVSAGACVIGRVEELGTAPL
LMTGLLGVERVLTMPSGMQLPRIC

Sequences:

>Translated_344_residues
MFECPVPEPVSDRIQMAHGGGGRLMNELIRSVFLSAFGCPSGGVQNDAAVLEIPPGRLAMTTDSFVVQPLEFPGGSIGSL
AVHGTVNDLAMSGAEPLYLTAGFILEEGLPLEVLARVAQDMAAAARAAGVRIVTGDTKVVERGKGDGIYINTAGVGIVRH
GLEISPSSVRPGDSVLLSGDLGRHGMTIMSLRAGLSFGDGLESDSAPLHESVAAVIRAGIPVHCLRDVTRGGLTATLSEI
AESAGLTVKLNEMSIPVREDVRAACGLLGLDPLQVACEGRYLAVLPREHEEEALNLMRGCGVSAGACVIGRVEELGTAPL
LMTGLLGVERVLTMPSGMQLPRIC
>Mature_344_residues
MFECPVPEPVSDRIQMAHGGGGRLMNELIRSVFLSAFGCPSGGVQNDAAVLEIPPGRLAMTTDSFVVQPLEFPGGSIGSL
AVHGTVNDLAMSGAEPLYLTAGFILEEGLPLEVLARVAQDMAAAARAAGVRIVTGDTKVVERGKGDGIYINTAGVGIVRH
GLEISPSSVRPGDSVLLSGDLGRHGMTIMSLRAGLSFGDGLESDSAPLHESVAAVIRAGIPVHCLRDVTRGGLTATLSEI
AESAGLTVKLNEMSIPVREDVRAACGLLGLDPLQVACEGRYLAVLPREHEEEALNLMRGCGVSAGACVIGRVEELGTAPL
LMTGLLGVERVLTMPSGMQLPRIC

Specific function: May be involved in the maturation of the nifE hydrogenase [H]

COG id: COG0309

COG function: function code O; Hydrogenase maturation factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypE family [H]

Homologues:

Organism=Escherichia coli, GI226510967, Length=339, Percent_Identity=45.1327433628319, Blast_Score=263, Evalue=1e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR011854
- InterPro:   IPR016188 [H]

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C [H]

EC number: NA

Molecular weight: Translated: 35692; Mature: 35692

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFECPVPEPVSDRIQMAHGGGGRLMNELIRSVFLSAFGCPSGGVQNDAAVLEIPPGRLAM
CCCCCCCCCHHHHEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEE
TTDSFVVQPLEFPGGSIGSLAVHGTVNDLAMSGAEPLYLTAGFILEEGLPLEVLARVAQD
ECCCCEEEEECCCCCCCCCEEEECCHHHHHHCCCCCEEEEECHHHCCCCCHHHHHHHHHH
MAAAARAAGVRIVTGDTKVVERGKGDGIYINTAGVGIVRHGLEISPSSVRPGDSVLLSGD
HHHHHHHCCEEEEECCHHHHHCCCCCEEEEEECCCEEHHCCCCCCCCCCCCCCCEEEECC
LGRHGMTIMSLRAGLSFGDGLESDSAPLHESVAAVIRAGIPVHCLRDVTRGGLTATLSEI
CCCCCCEEEHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHH
AESAGLTVKLNEMSIPVREDVRAACGLLGLDPLQVACEGRYLAVLPREHEEEALNLMRGC
HHHCCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHEECCCEEEECCCCCHHHHHHHHHHC
GVSAGACVIGRVEELGTAPLLMTGLLGVERVLTMPSGMQLPRIC
CCCCCHHHHHCHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MFECPVPEPVSDRIQMAHGGGGRLMNELIRSVFLSAFGCPSGGVQNDAAVLEIPPGRLAM
CCCCCCCCCHHHHEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEE
TTDSFVVQPLEFPGGSIGSLAVHGTVNDLAMSGAEPLYLTAGFILEEGLPLEVLARVAQD
ECCCCEEEEECCCCCCCCCEEEECCHHHHHHCCCCCEEEEECHHHCCCCCHHHHHHHHHH
MAAAARAAGVRIVTGDTKVVERGKGDGIYINTAGVGIVRHGLEISPSSVRPGDSVLLSGD
HHHHHHHCCEEEEECCHHHHHCCCCCEEEEEECCCEEHHCCCCCCCCCCCCCCCEEEECC
LGRHGMTIMSLRAGLSFGDGLESDSAPLHESVAAVIRAGIPVHCLRDVTRGGLTATLSEI
CCCCCCEEEHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCCHHHHHHHH
AESAGLTVKLNEMSIPVREDVRAACGLLGLDPLQVACEGRYLAVLPREHEEEALNLMRGC
HHHCCCEEEEECCCCCHHHHHHHHHCCCCCCHHHHEECCCEEEECCCCCHHHHHHHHHHC
GVSAGACVIGRVEELGTAPLLMTGLLGVERVLTMPSGMQLPRIC
CCCCCHHHHHCHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1526470 [H]