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The map label for this gene is cwlH [H]

Identifier: 187735738

GI number: 187735738

Start: 1493533

End: 1494264

Strand: Reverse

Name: cwlH [H]

Synonym: Amuc_1244

Alternate gene names: 187735738

Gene position: 1494264-1493533 (Counterclockwise)

Preceding gene: 187735739

Following gene: 187735731

Centisome position: 56.09

GC content: 56.42

Gene sequence:

>732_bases
ATGAGCAATATTTCCACCATCGCCACTTATCTTGCCCGCTTTTCCGGTCAGCTCCAGCCCATGCTGGAGAAAGTCCAGGG
CATGGACCGCAGAACTTTTCTGCTGGAAACCGCCGCCCTGCTTGCCCTGACCAGTTGTTCGGAACCTTCCGGAAAAGCAG
GAATGGCTCCCATGATCTCCTTTAAGCCCTCCGGGGTCCCCGTGGTGAAACGTTCTCCGCGCCAATTGCTGGCGGAATGT
AACGTCCGGCCGATGTTCATGCCTAAATCTTCTCCGCTCCGCCGCCGTTCTTCCCGAATGAAGCCGCGCTTCATCACCAT
GCACAACACGGAAAACCCGTCCGCAGACGCCATGCAGCATGCGCGCGCCCTGAATAACGGAGCTTTGCGCTGCAACTGGC
ATTATACGGTAGACCCATACGTGACCATGCAGCATATCCCCCTGAATGAAACGGGACGCCATGCAGACCGGGGCGGCCCC
GGAGACATGTACTCCATCGGAATTGAAATGTGTGAGAAGCGAGGACAGAGCATCGTCAAAACCTTTGACCGCGCTGCCAA
GCTGGCGGCCTACAACATGTATGCCCACGATATTCCCCTGAGGAATGTCGTGCCCCATTACTACTGGACCGGCAAGCGTT
GCCCCCACCTTCTGCTGGACAATGGCAAACCCGGCTTCAAATGGTCTTGGTTCATCTCCCGCGTGGATTATTATTACCGC
TGCATCAGCTGA

Upstream 100 bases:

>100_bases
GCTTTCACACTTTTTCGTTGCAAAGAAGAAGAGCGAATCTTAGCGTGAACTTAACTTTTCCCGCATCCGTGCTGAAAATT
CCCCATCTCCCGGCCTCCGT

Downstream 100 bases:

>100_bases
GATATCAGGCAACACCTCTTCCCTGTGCAGGGAAACGGGTAATATTATTATGCAGCCAAAAACGGCTTTGCCGCCAGGGA
ACGGTTAACAATTTCCTGCC

Product: N-acetylmuramoyl-L-alanine amidase family 2

Products: NA

Alternate protein names: Autolysin; Cell wall hydrolase [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MSNISTIATYLARFSGQLQPMLEKVQGMDRRTFLLETAALLALTSCSEPSGKAGMAPMISFKPSGVPVVKRSPRQLLAEC
NVRPMFMPKSSPLRRRSSRMKPRFITMHNTENPSADAMQHARALNNGALRCNWHYTVDPYVTMQHIPLNETGRHADRGGP
GDMYSIGIEMCEKRGQSIVKTFDRAAKLAAYNMYAHDIPLRNVVPHYYWTGKRCPHLLLDNGKPGFKWSWFISRVDYYYR
CIS

Sequences:

>Translated_243_residues
MSNISTIATYLARFSGQLQPMLEKVQGMDRRTFLLETAALLALTSCSEPSGKAGMAPMISFKPSGVPVVKRSPRQLLAEC
NVRPMFMPKSSPLRRRSSRMKPRFITMHNTENPSADAMQHARALNNGALRCNWHYTVDPYVTMQHIPLNETGRHADRGGP
GDMYSIGIEMCEKRGQSIVKTFDRAAKLAAYNMYAHDIPLRNVVPHYYWTGKRCPHLLLDNGKPGFKWSWFISRVDYYYR
CIS
>Mature_242_residues
SNISTIATYLARFSGQLQPMLEKVQGMDRRTFLLETAALLALTSCSEPSGKAGMAPMISFKPSGVPVVKRSPRQLLAECN
VRPMFMPKSSPLRRRSSRMKPRFITMHNTENPSADAMQHARALNNGALRCNWHYTVDPYVTMQHIPLNETGRHADRGGPG
DMYSIGIEMCEKRGQSIVKTFDRAAKLAAYNMYAHDIPLRNVVPHYYWTGKRCPHLLLDNGKPGFKWSWFISRVDYYYRC
IS

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella and sporulation. Could play a role in mother cell lysis with CwlC [H]

COG id: COG5632

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2; PF01471 PG_binding_1 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 27634; Mature: 27503

Theoretical pI: Translated: 10.24; Mature: 10.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
8.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
7.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNISTIATYLARFSGQLQPMLEKVQGMDRRTFLLETAALLALTSCSEPSGKAGMAPMIS
CCCHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
FKPSGVPVVKRSPRQLLAECNVRPMFMPKSSPLRRRSSRMKPRFITMHNTENPSADAMQH
ECCCCCCCCCCCHHHHHHHCCCCEEECCCCCHHHHHHHCCCCEEEEEECCCCCCHHHHHH
ARALNNGALRCNWHYTVDPYVTMQHIPLNETGRHADRGGPGDMYSIGIEMCEKRGQSIVK
HHHHCCCEEEEEEEEEECCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
TFDRAAKLAAYNMYAHDIPLRNVVPHYYWTGKRCPHLLLDNGKPGFKWSWFISRVDYYYR
HHHHHHHHHHHHHHHHCCCHHHCCCCEEECCCCCCEEEECCCCCCCCHHHHHHHHHHHHH
CIS
CCC
>Mature Secondary Structure 
SNISTIATYLARFSGQLQPMLEKVQGMDRRTFLLETAALLALTSCSEPSGKAGMAPMIS
CCHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEE
FKPSGVPVVKRSPRQLLAECNVRPMFMPKSSPLRRRSSRMKPRFITMHNTENPSADAMQH
ECCCCCCCCCCCHHHHHHHCCCCEEECCCCCHHHHHHHCCCCEEEEEECCCCCCHHHHHH
ARALNNGALRCNWHYTVDPYVTMQHIPLNETGRHADRGGPGDMYSIGIEMCEKRGQSIVK
HHHHCCCEEEEEEEEEECCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
TFDRAAKLAAYNMYAHDIPLRNVVPHYYWTGKRCPHLLLDNGKPGFKWSWFISRVDYYYR
HHHHHHHHHHHHHHHHCCCHHHCCCCEEECCCCCCEEEECCCCCCCCHHHHHHHHHHHHH
CIS
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]