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The map label for this gene is ispF

Identifier: 187735737

GI number: 187735737

Start: 1492774

End: 1493283

Strand: Direct

Name: ispF

Synonym: Amuc_1243

Alternate gene names: 187735737

Gene position: 1492774-1493283 (Clockwise)

Preceding gene: 187735736

Following gene: 187735747

Centisome position: 56.03

GC content: 58.43

Gene sequence:

>510_bases
ATGAAAAACATGATCGCTTTGACCGGGATCGGGTATGATATCCACCGTTTTGCGGAAGTTCCGCGCCCCTTGATGCTGGG
CGGTGTTCATATTCCTTCCTCCCGCGGCCTGGACGGCCATTCCGACGCGGATGTTTTATGCCACGCCATTGCGGATGCCC
TGCTGGGCGCTGCCGGGTTGCCGGACATCGGCTACTGGTTTCCGCCGGGCGATCCTTCCTGCAAGGACATCCCTTCCCTG
GATATTGTGGCGAAAGCCGTTTCTCTGATTCGGGAGCGCGGCGGCCGCATCGTGAATGTGGACTCGTCCCTTATTGCAGA
ATCTCCCCGTATTTCCCCTTATTTGGAGCAGATGAAGGGAGCTTTGGGCGCCGCTCTGGGAATCTCTCCGGTCCGTGTGG
GCATCAAGGCCACTACTAATGAACAGCTTGGCGCGCTGGGCCGCAGGGAAGGCATTGCCGCTTTTGCCGTAGCGGCTATT
CTGATGCCGGAGGATTTGACCATGCCGTAA

Upstream 100 bases:

>100_bases
AAGATAATCCGGAACAATTCGCCATTGTCGCGTGGAACGGCCGCATCCCTATCATGGAGGCGCTGGGGGAGTAACCCGAA
CATTATTGCCGCAGTACCGT

Downstream 100 bases:

>100_bases
GAAGAACGCTCCGGTTCCGTTTCTGGAAGCCTTTTTTCAAAAAAGAAGCCGATATGGGGCTTTAAACAAGGAATATGCTT
CAGGAATAACGGGCAGGTGG

Product: 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MKNMIALTGIGYDIHRFAEVPRPLMLGGVHIPSSRGLDGHSDADVLCHAIADALLGAAGLPDIGYWFPPGDPSCKDIPSL
DIVAKAVSLIRERGGRIVNVDSSLIAESPRISPYLEQMKGALGAALGISPVRVGIKATTNEQLGALGRREGIAAFAVAAI
LMPEDLTMP

Sequences:

>Translated_169_residues
MKNMIALTGIGYDIHRFAEVPRPLMLGGVHIPSSRGLDGHSDADVLCHAIADALLGAAGLPDIGYWFPPGDPSCKDIPSL
DIVAKAVSLIRERGGRIVNVDSSLIAESPRISPYLEQMKGALGAALGISPVRVGIKATTNEQLGALGRREGIAAFAVAAI
LMPEDLTMP
>Mature_169_residues
MKNMIALTGIGYDIHRFAEVPRPLMLGGVHIPSSRGLDGHSDADVLCHAIADALLGAAGLPDIGYWFPPGDPSCKDIPSL
DIVAKAVSLIRERGGRIVNVDSSLIAESPRISPYLEQMKGALGAALGISPVRVGIKATTNEQLGALGRREGIAAFAVAAI
LMPEDLTMP

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=153, Percent_Identity=43.1372549019608, Blast_Score=112, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_AKKM8 (B2URI3)

Other databases:

- EMBL:   CP001071
- RefSeq:   YP_001877849.1
- ProteinModelPortal:   B2URI3
- SMR:   B2URI3
- GeneID:   6275853
- GenomeReviews:   CP001071_GR
- KEGG:   amu:Amuc_1243
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 17689; Mature: 17689

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNMIALTGIGYDIHRFAEVPRPLMLGGVHIPSSRGLDGHSDADVLCHAIADALLGAAGL
CCCEEEEECCCHHHHHHHHCCCCEEECCEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
PDIGYWFPPGDPSCKDIPSLDIVAKAVSLIRERGGRIVNVDSSLIAESPRISPYLEQMKG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECHHHHHCCCCCCHHHHHHHH
ALGAALGISPVRVGIKATTNEQLGALGRREGIAAFAVAAILMPEDLTMP
HHHHHHCCCEEEEEEEECCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKNMIALTGIGYDIHRFAEVPRPLMLGGVHIPSSRGLDGHSDADVLCHAIADALLGAAGL
CCCEEEEECCCHHHHHHHHCCCCEEECCEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
PDIGYWFPPGDPSCKDIPSLDIVAKAVSLIRERGGRIVNVDSSLIAESPRISPYLEQMKG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEECHHHHHCCCCCCHHHHHHHH
ALGAALGISPVRVGIKATTNEQLGALGRREGIAAFAVAAILMPEDLTMP
HHHHHHCCCEEEEEEEECCCHHHHHHHHHCCHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA