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The map label for this gene is ispE [H]

Identifier: 187735689

GI number: 187735689

Start: 1431708

End: 1432556

Strand: Direct

Name: ispE [H]

Synonym: Amuc_1195

Alternate gene names: 187735689

Gene position: 1431708-1432556 (Clockwise)

Preceding gene: 187735684

Following gene: 187735690

Centisome position: 53.74

GC content: 63.37

Gene sequence:

>849_bases
ATGATCAGTTGCAAGGCTCCGTGCAAAGTGAACGTTTCCCTCCGCGTTCTGGGAAAGAGGCCGGACGGCTTCCATGAGGT
GGATACCGTGATGGTTCCCTTGGATTTGTGTGATGTGCTGGAGTTTTCGCCGGCGGCCTGTCTGGAGATGAGCTGCGACG
CCCCGGGCGTGCCTCTGGATGAGAGCAACCTGGTCATGAAAGCGGGCCGGCTGATGGAGCGGGAGCTGGGAAGGCCCATG
CCGTGGCATGTCCGCCTGGTGAAAAAGGTGCCCCATGGCGCGGGGCTGGGCGGCGGCAGCAGTGACGCCGCATGCGTGCT
GTGCACCCTCAATGAGCTGGAACGCGGAGGTTTGTCCCGGGAGCGCCTGGCGGAGCTGGGCGGGGAAATCGGTTCCGACG
TGGGGTTTTTCATTTATGGCGCGGCGAGCCGCTGCACCGGGCGCGGAGAGAAGGTGGAGCCCTTGCCGGAGTGGAAGGGG
TGGCGTCCCCGGGTTGTCTTGTTGAAGCCGTCGTTTGGTGTCTCCACGCCGGACGCTTACCGCCGCTGGTCCGGTTCCAG
GGAATTGCCCGGCATTCCCTATGGGGAGCAGGATGTGGACGGCCATGTGCTAGTCAATGATCTGGAAAGGCCTGTGTTTG
AAAAGCATTTATTCCTGGCGGAAGTGAAGCGCTGGCTGATGGGGCGGCCCGGGGTGCGCGGCGCCATGATGTCCGGTTCC
GGTTCCACCATGTTCGCCGTGGTGGAGGATGAAGGAACCGGACGCGCGCTGATGGAGGATGCCGCCCGGGAGCTGGACCC
CACTTTATGGATGTGGTCCGGCCTGGTGATGCAGGATGACGCGCGGTAA

Upstream 100 bases:

>100_bases
AGCGGCGCCCCCGGAATTTGCGAAGAAAAAATGAGGGGCTGTCCGTCTGCGTTCGATGGAGCTTGACCCGGAGGCCGTCC
CTTGGTGGAATCCACGGCGT

Downstream 100 bases:

>100_bases
AAACGTGATTCCGCAGGAGGCCTTGCCTTGACGGAGGGGGAGGCATGCCGTTACACTCCGTCCATGATTAAGAGTTTGTT
GGCAGGCATGATGGTGGCGG

Product: 4-diphosphocytidyl-2C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase [H]

Number of amino acids: Translated: 282; Mature: 282

Protein sequence:

>282_residues
MISCKAPCKVNVSLRVLGKRPDGFHEVDTVMVPLDLCDVLEFSPAACLEMSCDAPGVPLDESNLVMKAGRLMERELGRPM
PWHVRLVKKVPHGAGLGGGSSDAACVLCTLNELERGGLSRERLAELGGEIGSDVGFFIYGAASRCTGRGEKVEPLPEWKG
WRPRVVLLKPSFGVSTPDAYRRWSGSRELPGIPYGEQDVDGHVLVNDLERPVFEKHLFLAEVKRWLMGRPGVRGAMMSGS
GSTMFAVVEDEGTGRALMEDAARELDPTLWMWSGLVMQDDAR

Sequences:

>Translated_282_residues
MISCKAPCKVNVSLRVLGKRPDGFHEVDTVMVPLDLCDVLEFSPAACLEMSCDAPGVPLDESNLVMKAGRLMERELGRPM
PWHVRLVKKVPHGAGLGGGSSDAACVLCTLNELERGGLSRERLAELGGEIGSDVGFFIYGAASRCTGRGEKVEPLPEWKG
WRPRVVLLKPSFGVSTPDAYRRWSGSRELPGIPYGEQDVDGHVLVNDLERPVFEKHLFLAEVKRWLMGRPGVRGAMMSGS
GSTMFAVVEDEGTGRALMEDAARELDPTLWMWSGLVMQDDAR
>Mature_282_residues
MISCKAPCKVNVSLRVLGKRPDGFHEVDTVMVPLDLCDVLEFSPAACLEMSCDAPGVPLDESNLVMKAGRLMERELGRPM
PWHVRLVKKVPHGAGLGGGSSDAACVLCTLNELERGGLSRERLAELGGEIGSDVGFFIYGAASRCTGRGEKVEPLPEWKG
WRPRVVLLKPSFGVSTPDAYRRWSGSRELPGIPYGEQDVDGHVLVNDLERPVFEKHLFLAEVKRWLMGRPGVRGAMMSGS
GSTMFAVVEDEGTGRALMEDAARELDPTLWMWSGLVMQDDAR

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol [H]

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787459, Length=273, Percent_Identity=29.6703296703297, Blast_Score=89, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721 [H]

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N [H]

EC number: =2.7.1.148 [H]

Molecular weight: Translated: 30837; Mature: 30837

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISCKAPCKVNVSLRVLGKRPDGFHEVDTVMVPLDLCDVLEFSPAACLEMSCDAPGVPLD
CCCCCCCCEEEEEEEEEECCCCCCCCCCEEEECHHHHHHHHCCCCCEEEECCCCCCCCCC
ESNLVMKAGRLMERELGRPMPWHVRLVKKVPHGAGLGGGSSDAACVLCTLNELERGGLSR
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHCCCCH
ERLAELGGEIGSDVGFFIYGAASRCTGRGEKVEPLPEWKGWRPRVVLLKPSFGVSTPDAY
HHHHHHHHHHCCCCEEEEEECCHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHH
RRWSGSRELPGIPYGEQDVDGHVLVNDLERPVFEKHLFLAEVKRWLMGRPGVRGAMMSGS
HHCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCC
GSTMFAVVEDEGTGRALMEDAARELDPTLWMWSGLVMQDDAR
CCEEEEEEECCCCCHHHHHHHHHHCCCCEEECCCEEEECCCC
>Mature Secondary Structure
MISCKAPCKVNVSLRVLGKRPDGFHEVDTVMVPLDLCDVLEFSPAACLEMSCDAPGVPLD
CCCCCCCCEEEEEEEEEECCCCCCCCCCEEEECHHHHHHHHCCCCCEEEECCCCCCCCCC
ESNLVMKAGRLMERELGRPMPWHVRLVKKVPHGAGLGGGSSDAACVLCTLNELERGGLSR
CCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHCCCCH
ERLAELGGEIGSDVGFFIYGAASRCTGRGEKVEPLPEWKGWRPRVVLLKPSFGVSTPDAY
HHHHHHHHHHCCCCEEEEEECCHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCCHHH
RRWSGSRELPGIPYGEQDVDGHVLVNDLERPVFEKHLFLAEVKRWLMGRPGVRGAMMSGS
HHCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCC
GSTMFAVVEDEGTGRALMEDAARELDPTLWMWSGLVMQDDAR
CCEEEEEEECCCCCHHHHHHHHHHCCCCEEECCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA