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The map label for this gene is metF [H]

Identifier: 187735550

GI number: 187735550

Start: 1259612

End: 1260547

Strand: Direct

Name: metF [H]

Synonym: Amuc_1055

Alternate gene names: 187735550

Gene position: 1259612-1260547 (Clockwise)

Preceding gene: 187735549

Following gene: 187735551

Centisome position: 47.28

GC content: 57.37

Gene sequence:

>936_bases
GTGCACCTGAGAGAAAGATTGCAATCGGGCGCCGGTCCCAGCCTGTCCTTTGAATTTTTCCCGCCCAAAAACGAGCAGGC
AGCGTCGGCCTTGTACCAGACTATCCGTGAACTGGAGCCTTACCGCCCCAGTTTCGTCAGCGTGACGTATGGTGCAGGGG
GCTCCACCCGCGAGCTTACCCGCGAGGTGGTGTTGCGCATCCAGCATGAATCCCGTATTCCTACGGTTCCCCACCTTACT
TGCGTAGGGCATAGCCGGGAGGAGGTATGGAATATCCTGGACCAATACGCCCGGGCTGGAGTGCGGGCCGTTCTTGCACT
TCGGGGAGATCCTCCCCGGGGAGCGAAGGATTACAACCGCGATGCGGATGTTTTTCGCCATGCGGCGGATCTGGTGACGT
TTATCCGTGAATATAATGAATCCGGCCGTCACCCGGATCCGGATGGATTCGCCATAGGCGTGGCCGGCTTCCCGGAAGGT
CATCCGGCCACTCCCAACCGCCTGCGCGAGATGGATTTCCTGAAAGCCAAGGTGGATGCCGGCGCGGATTATATCTGCAC
CCAGCTGTTTTTTGACAATCATTCTTTTCTGGATTACCGGGAACGCTGTCTGCTGGCCGGCATCAAGGTGCCTATCCTGG
CGGGAATTATGCCTGTGACCAGCCTGTCCGGCATGAACCGCATGGCAGAGCTTTCCGGAGGCACCTGTTTTCCGGCCAGG
CTGCTCAAGGCGCTGAAGCGTGCGGGTTCCAATCCGGACGCTATTCAGGAAGTAGGCATCCAATACGCCTCCAGCCAGTG
TGCCGAACTGTTGGATTCCGATGTGGACGGCATTCATTTTTATACGCTTAACCAGAGCCGGGCAACGCGGGAGATCTACA
GGAATCTGGGACTGAAGGATTCCCTGCAGCTTTTGGAACATGGCGAAGATAAGTAA

Upstream 100 bases:

>100_bases
TTCTCCCTCCGCCCCCGTCCCCGTGGAAGCTCCCAAACCGGTGATGATTCCCATGGAGCCGTGATGCGGGTCCGTATTTA
GCCAAGCCAAGTAAAGAGAA

Downstream 100 bases:

>100_bases
AAAGCTGATTGATTTGGTTGGGACAACACGGTAGAAACCTCTTCAACATATTATGGATATCAAGGATATTAAACGGGCCA
TGGACAATATGCCGATGGGC

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 311

Protein sequence:

>311_residues
MHLRERLQSGAGPSLSFEFFPPKNEQAASALYQTIRELEPYRPSFVSVTYGAGGSTRELTREVVLRIQHESRIPTVPHLT
CVGHSREEVWNILDQYARAGVRAVLALRGDPPRGAKDYNRDADVFRHAADLVTFIREYNESGRHPDPDGFAIGVAGFPEG
HPATPNRLREMDFLKAKVDAGADYICTQLFFDNHSFLDYRERCLLAGIKVPILAGIMPVTSLSGMNRMAELSGGTCFPAR
LLKALKRAGSNPDAIQEVGIQYASSQCAELLDSDVDGIHFYTLNQSRATREIYRNLGLKDSLQLLEHGEDK

Sequences:

>Translated_311_residues
MHLRERLQSGAGPSLSFEFFPPKNEQAASALYQTIRELEPYRPSFVSVTYGAGGSTRELTREVVLRIQHESRIPTVPHLT
CVGHSREEVWNILDQYARAGVRAVLALRGDPPRGAKDYNRDADVFRHAADLVTFIREYNESGRHPDPDGFAIGVAGFPEG
HPATPNRLREMDFLKAKVDAGADYICTQLFFDNHSFLDYRERCLLAGIKVPILAGIMPVTSLSGMNRMAELSGGTCFPAR
LLKALKRAGSNPDAIQEVGIQYASSQCAELLDSDVDGIHFYTLNQSRATREIYRNLGLKDSLQLLEHGEDK
>Mature_311_residues
MHLRERLQSGAGPSLSFEFFPPKNEQAASALYQTIRELEPYRPSFVSVTYGAGGSTRELTREVVLRIQHESRIPTVPHLT
CVGHSREEVWNILDQYARAGVRAVLALRGDPPRGAKDYNRDADVFRHAADLVTFIREYNESGRHPDPDGFAIGVAGFPEG
HPATPNRLREMDFLKAKVDAGADYICTQLFFDNHSFLDYRERCLLAGIKVPILAGIMPVTSLSGMNRMAELSGGTCFPAR
LLKALKRAGSNPDAIQEVGIQYASSQCAELLDSDVDGIHFYTLNQSRATREIYRNLGLKDSLQLLEHGEDK

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=301, Percent_Identity=34.5514950166113, Blast_Score=160, Evalue=2e-39,
Organism=Escherichia coli, GI1790377, Length=289, Percent_Identity=33.9100346020761, Blast_Score=172, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI25147441, Length=305, Percent_Identity=33.4426229508197, Blast_Score=160, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI25147436, Length=305, Percent_Identity=33.4426229508197, Blast_Score=160, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6325234, Length=308, Percent_Identity=34.4155844155844, Blast_Score=174, Evalue=2e-44,
Organism=Saccharomyces cerevisiae, GI6321313, Length=306, Percent_Identity=32.3529411764706, Blast_Score=164, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24662658, Length=287, Percent_Identity=29.2682926829268, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 34605; Mature: 34605

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLRERLQSGAGPSLSFEFFPPKNEQAASALYQTIRELEPYRPSFVSVTYGAGGSTRELT
CCHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHH
REVVLRIQHESRIPTVPHLTCVGHSREEVWNILDQYARAGVRAVLALRGDPPRGAKDYNR
HHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCC
DADVFRHAADLVTFIREYNESGRHPDPDGFAIGVAGFPEGHPATPNRLREMDFLKAKVDA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCC
GADYICTQLFFDNHSFLDYRERCLLAGIKVPILAGIMPVTSLSGMNRMAELSGGTCFPAR
CCHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
LLKALKRAGSNPDAIQEVGIQYASSQCAELLDSDVDGIHFYTLNQSRATREIYRNLGLKD
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCHH
SLQLLEHGEDK
HHHHHHCCCCC
>Mature Secondary Structure
MHLRERLQSGAGPSLSFEFFPPKNEQAASALYQTIRELEPYRPSFVSVTYGAGGSTRELT
CCHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHH
REVVLRIQHESRIPTVPHLTCVGHSREEVWNILDQYARAGVRAVLALRGDPPRGAKDYNR
HHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCC
DADVFRHAADLVTFIREYNESGRHPDPDGFAIGVAGFPEGHPATPNRLREMDFLKAKVDA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHCC
GADYICTQLFFDNHSFLDYRERCLLAGIKVPILAGIMPVTSLSGMNRMAELSGGTCFPAR
CCHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
LLKALKRAGSNPDAIQEVGIQYASSQCAELLDSDVDGIHFYTLNQSRATREIYRNLGLKD
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHCCCHH
SLQLLEHGEDK
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]