Click here to switch to the map view.

The map label for this gene is nusA [H]

Identifier: 187735458

GI number: 187735458

Start: 1142034

End: 1143329

Strand: Reverse

Name: nusA [H]

Synonym: Amuc_0957

Alternate gene names: 187735458

Gene position: 1143329-1142034 (Counterclockwise)

Preceding gene: 187735461

Following gene: 187735457

Centisome position: 42.92

GC content: 58.18

Gene sequence:

>1296_bases
ATGACAAACGATATTAAAGCTTTGATTGACTACTACGAGAGGGAAAAAGGGCTCTCCCGTGAAAAAATTCTCCTCGCTCT
GGAGTCCGCCTTTCTCTCCGCCTACCGCAAAATGGTTCCCGGTTCCGGCAGCATCAACTACCTGAGGGCCGAAATCAACG
TGGACAAGGGCAAGGTGCGTATTTTTGCGGACCTGGAAGTGGTGCCGGATGAAGAATATTCCGACAAATTCAACCAGATC
CCCCTTTCCCTGGCCGTCAAGCTGGACAAAAACGCCGTGCTTCACGACCTGCTCCCCACCAACATCACGCCCAAGGGCTT
CGGCCGCATCGCGGTGCAGACCGCCCGCCAGACCATGCTCCAGAAACTGCTGGATGCGGAAAAGGAAATGCTCTACGACG
AATTCAAGGACCGCGCCGGAGATCTGGTAACGGGCACCATCCGCCGTTTTGAAAAGGGGGATATTTTTGTGGACCTCGGC
AAATTCGAAGGCGTCATGACCTCCCGCGAACGCGTGCCGAATGAAGACTACAGCGTCGGCGACCGCATGCGCTTCTACGT
GGTGGAAGTGCGCACGGAAGCACGCGGCCCGGAAGTCATCCTTTCCCGCAGCCATCCGAACCTGGTGCGCCGCCTCTTTG
AATCGGAAGTGGTGGAAATAGGCGACCAGACCGTGGAAATCCACGGCATCGCCCGCGAAGCCGGCTACCGCACCAAAGTG
GCCGTCATCAGCCATGACGACAAAGTAGATCCGGTAGGGGCATGCGTAGGCATGCGCGGCGCCCGCGTCAAAAACATCGT
CCGGGAGCTCAACAATGAAAAAGTGGACATCCTGGAATGGACGGAAGACCCCGTCACCTTCGTCCGGGAAGCTCTCAGCC
CCGTGGAACCGCGGGAAATCACCGTGGACGAGGAAGCCAGAAAAATCTTCGTTATCGTCCAGGACGACAAAGACCTCTCC
AAGGCCATCGGCCGCAGGGGCCAGAATGCCCGCCTCACCTCCCGCCTGATGGGCTGGGATGTCCAGGTGCGCGTCTTTGA
TGTCCAGGAAGCGGAAAAACGCCAGAGCCAGGCTGCGGCCGAAGAAGTCATGCGCCAATGCCAGGCTGCGGCCAAAACCC
TCAGCGAACAATTGGAAATCCCGGAAGAAACCGCCATGGGCCTGGTGACCATGGGCGGAACGGACCTGGTGGCCCTCACC
GGATTTGAAGCTTCCGACATCGCGGAAAGCATGGGCATTCCCGCAGAGGAAGCCGCCCAAATTCTGGACAAGGCCCGGGA
CCTTATCTCCCAATAA

Upstream 100 bases:

>100_bases
CCCTCGTGCTTACTTAAATAGGCAAACACCCCGAGAGGCACTCGAGAGTGGGTCTGTCGACCCGCTCTTTTTTGTCTATC
CATCACACTCCTCCACACTC

Downstream 100 bases:

>100_bases
CCGTTGAGCGCGGCGCTCAACATCTAACAACCTTAAATCAAGATGCCTAATAAACAAGAAGAGAAAGAACCCAAAAAGGA
AGTATTGGATCTGATCGGCG

Product: NusA antitermination factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MTNDIKALIDYYEREKGLSREKILLALESAFLSAYRKMVPGSGSINYLRAEINVDKGKVRIFADLEVVPDEEYSDKFNQI
PLSLAVKLDKNAVLHDLLPTNITPKGFGRIAVQTARQTMLQKLLDAEKEMLYDEFKDRAGDLVTGTIRRFEKGDIFVDLG
KFEGVMTSRERVPNEDYSVGDRMRFYVVEVRTEARGPEVILSRSHPNLVRRLFESEVVEIGDQTVEIHGIAREAGYRTKV
AVISHDDKVDPVGACVGMRGARVKNIVRELNNEKVDILEWTEDPVTFVREALSPVEPREITVDEEARKIFVIVQDDKDLS
KAIGRRGQNARLTSRLMGWDVQVRVFDVQEAEKRQSQAAAEEVMRQCQAAAKTLSEQLEIPEETAMGLVTMGGTDLVALT
GFEASDIAESMGIPAEEAAQILDKARDLISQ

Sequences:

>Translated_431_residues
MTNDIKALIDYYEREKGLSREKILLALESAFLSAYRKMVPGSGSINYLRAEINVDKGKVRIFADLEVVPDEEYSDKFNQI
PLSLAVKLDKNAVLHDLLPTNITPKGFGRIAVQTARQTMLQKLLDAEKEMLYDEFKDRAGDLVTGTIRRFEKGDIFVDLG
KFEGVMTSRERVPNEDYSVGDRMRFYVVEVRTEARGPEVILSRSHPNLVRRLFESEVVEIGDQTVEIHGIAREAGYRTKV
AVISHDDKVDPVGACVGMRGARVKNIVRELNNEKVDILEWTEDPVTFVREALSPVEPREITVDEEARKIFVIVQDDKDLS
KAIGRRGQNARLTSRLMGWDVQVRVFDVQEAEKRQSQAAAEEVMRQCQAAAKTLSEQLEIPEETAMGLVTMGGTDLVALT
GFEASDIAESMGIPAEEAAQILDKARDLISQ
>Mature_430_residues
TNDIKALIDYYEREKGLSREKILLALESAFLSAYRKMVPGSGSINYLRAEINVDKGKVRIFADLEVVPDEEYSDKFNQIP
LSLAVKLDKNAVLHDLLPTNITPKGFGRIAVQTARQTMLQKLLDAEKEMLYDEFKDRAGDLVTGTIRRFEKGDIFVDLGK
FEGVMTSRERVPNEDYSVGDRMRFYVVEVRTEARGPEVILSRSHPNLVRRLFESEVVEIGDQTVEIHGIAREAGYRTKVA
VISHDDKVDPVGACVGMRGARVKNIVRELNNEKVDILEWTEDPVTFVREALSPVEPREITVDEEARKIFVIVQDDKDLSK
AIGRRGQNARLTSRLMGWDVQVRVFDVQEAEKRQSQAAAEEVMRQCQAAAKTLSEQLEIPEETAMGLVTMGGTDLVALTG
FEASDIAESMGIPAEEAAQILDKARDLISQ

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=431, Percent_Identity=35.0348027842227, Blast_Score=244, Evalue=8e-66,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 48377; Mature: 48246

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNDIKALIDYYEREKGLSREKILLALESAFLSAYRKMVPGSGSINYLRAEINVDKGKVR
CCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCEEE
IFADLEVVPDEEYSDKFNQIPLSLAVKLDKNAVLHDLLPTNITPKGFGRIAVQTARQTML
EEEEEEECCCCHHHHHHHCCCEEEEEEECCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
QKLLDAEKEMLYDEFKDRAGDLVTGTIRRFEKGDIFVDLGKFEGVMTSRERVPNEDYSVG
HHHHHHHHHHHHHHHHHHCCCEEHHHHHHCCCCCEEEECCCCCCHHHHHHHCCCCCCCCC
DRMRFYVVEVRTEARGPEVILSRSHPNLVRRLFESEVVEIGDQTVEIHGIAREAGYRTKV
CCEEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHCCCCEEE
AVISHDDKVDPVGACVGMRGARVKNIVRELNNEKVDILEWTEDPVTFVREALSPVEPREI
EEEECCCCCCHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCEE
TVDEEARKIFVIVQDDKDLSKAIGRRGQNARLTSRLMGWDVQVRVFDVQEAEKRQSQAAA
EECCCCCEEEEEEECCHHHHHHHHCCCCCCHHHHHHHCCEEEEEEEECHHHHHHHHHHHH
EEVMRQCQAAAKTLSEQLEIPEETAMGLVTMGGTDLVALTGFEASDIAESMGIPAEEAAQ
HHHHHHHHHHHHHHHHHHCCCHHHHCEEEEECCCCEEEEECCCHHHHHHHHCCCHHHHHH
ILDKARDLISQ
HHHHHHHHHCC
>Mature Secondary Structure 
TNDIKALIDYYEREKGLSREKILLALESAFLSAYRKMVPGSGSINYLRAEINVDKGKVR
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCEEE
IFADLEVVPDEEYSDKFNQIPLSLAVKLDKNAVLHDLLPTNITPKGFGRIAVQTARQTML
EEEEEEECCCCHHHHHHHCCCEEEEEEECCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHH
QKLLDAEKEMLYDEFKDRAGDLVTGTIRRFEKGDIFVDLGKFEGVMTSRERVPNEDYSVG
HHHHHHHHHHHHHHHHHHCCCEEHHHHHHCCCCCEEEECCCCCCHHHHHHHCCCCCCCCC
DRMRFYVVEVRTEARGPEVILSRSHPNLVRRLFESEVVEIGDQTVEIHGIAREAGYRTKV
CCEEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHHCCCCEEEEEEEHHHCCCCEEE
AVISHDDKVDPVGACVGMRGARVKNIVRELNNEKVDILEWTEDPVTFVREALSPVEPREI
EEEECCCCCCHHHHHHCCCCHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCCEE
TVDEEARKIFVIVQDDKDLSKAIGRRGQNARLTSRLMGWDVQVRVFDVQEAEKRQSQAAA
EECCCCCEEEEEEECCHHHHHHHHCCCCCCHHHHHHHCCEEEEEEEECHHHHHHHHHHHH
EEVMRQCQAAAKTLSEQLEIPEETAMGLVTMGGTDLVALTGFEASDIAESMGIPAEEAAQ
HHHHHHHHHHHHHHHHHHCCCHHHHCEEEEECCCCEEEEECCCHHHHHHHHCCCHHHHHH
ILDKARDLISQ
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]