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The map label for this gene is mdtE [H]

Identifier: 187735391

GI number: 187735391

Start: 1058478

End: 1059632

Strand: Direct

Name: mdtE [H]

Synonym: Amuc_0889

Alternate gene names: 187735391

Gene position: 1058478-1059632 (Clockwise)

Preceding gene: 187735385

Following gene: 187735392

Centisome position: 39.73

GC content: 62.25

Gene sequence:

>1155_bases
ATGAAAATAATTAATCTTTTATTCTGCATGCCCCTGCTGGTGGTTTGCTTCTCCTGCCAGCGGGAGGGTGAAAGCCAGGC
AGCCGGATTGCCCGGCGAAACCGTGCCGGAAGTGTCTGTCATGACGGTACGCAGTACTCAGGTTCCCGTAACCGCCAATC
TTCCCGGCCGTATGGAAGCGTATCTCCAGGCGGAAGTGCGCGCCCGCGTGACGGGAATTATTCAGGAACGCTGCTATCAG
GAAGGGCAGACGGTCCGGCCGGGCGATCTTCTTTTTAAAATAGACCCGGCTCCGCTTCAGGCTGTCCTGGATGAATGCAA
GGCTGCAGTGGCTCGTGCCAGGGCCGTTCTGTCGGATGCGGAGGACAAGGCCGCCCGGTATTCCTCCCTGGTCGCCAAGG
GCGCCGTCAGCATACGTGAGCATAAACAGGCCAGGGCGGAGGAAGAGCGGGCCAGGGCGGAATATGCCGCCGCGGCCGCC
TCTCTGGAGCAGGCGCGCCTGAATCTGGAATATACCCGAGTGGAGGCGCCCATCTCCGGCCGCGTGCGCCGTGCCCTGGT
GACGGAAGGGGCGTTTGCCAACCAGAATGAATTCACCCACCTGACCACCATTGAACAGATTGACCCCATTTATGTCCGCT
TCAGCCAGCCGGCCTCCCAGTACAGCAGCCTGCGCCGCGCCGTCGTATCCGGATTGTGGAAGGGAGTGCCGCTGGGCGAA
ATCAAGGTGCGCCTGCTGCTCTCCAATGGGGAGGAATACCCCCATTCCGGCAGGATCATTTTTTCCGACATGGCGGTGGA
CCCGAATACGGATACTATTGAAATGCGGGCCCTGTTCCCCAACCCGGACCAAGAACTGCTGCCCGGCGCCTATGTGCGCG
TGGTGTTTGACAGGGCGGTGCGGGACAACGTATTCGCCATTCCCCGGGATGCCGTCATCCGCACCGCGCAGGGAGCTTCC
GTCTTTGTCGTGGGCCCGGAAGGCGTGCTGGAAGCGCGCCCCGTCAGGGCGGATACGCTGAACGGCCGGGAATGGCTGGT
TTCAGAAGGCCTCCGGGATGGGGACAGGGTTGCCGTCAGCCACACGATGTCCCTCCGCCCCGGCATGAAAGTCCGCAGTG
CCGCCGCCCGGCCGCAACCCCATGCCCAGCAATAA

Upstream 100 bases:

>100_bases
ATTATGGGATACTTTACATCATATGGCAATTGAAAAATCCCCGGTCTTGCCGGAAGTGCGGTTCCGGGGTTTCTTGAGGT
TCCAAACAGCATGATTCCTG

Downstream 100 bases:

>100_bases
ACCGCCATGCCTGATTTCTTCATACGCCGCCCCATTTTTGCCTGGGTAGTCGCACTCCTGATTTCCCTGATGGGCCTTCT
GGCCATTCCCAGCCTGCCCA

Product: efflux transporter, RND family, MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MKIINLLFCMPLLVVCFSCQREGESQAAGLPGETVPEVSVMTVRSTQVPVTANLPGRMEAYLQAEVRARVTGIIQERCYQ
EGQTVRPGDLLFKIDPAPLQAVLDECKAAVARARAVLSDAEDKAARYSSLVAKGAVSIREHKQARAEEERARAEYAAAAA
SLEQARLNLEYTRVEAPISGRVRRALVTEGAFANQNEFTHLTTIEQIDPIYVRFSQPASQYSSLRRAVVSGLWKGVPLGE
IKVRLLLSNGEEYPHSGRIIFSDMAVDPNTDTIEMRALFPNPDQELLPGAYVRVVFDRAVRDNVFAIPRDAVIRTAQGAS
VFVVGPEGVLEARPVRADTLNGREWLVSEGLRDGDRVAVSHTMSLRPGMKVRSAAARPQPHAQQ

Sequences:

>Translated_384_residues
MKIINLLFCMPLLVVCFSCQREGESQAAGLPGETVPEVSVMTVRSTQVPVTANLPGRMEAYLQAEVRARVTGIIQERCYQ
EGQTVRPGDLLFKIDPAPLQAVLDECKAAVARARAVLSDAEDKAARYSSLVAKGAVSIREHKQARAEEERARAEYAAAAA
SLEQARLNLEYTRVEAPISGRVRRALVTEGAFANQNEFTHLTTIEQIDPIYVRFSQPASQYSSLRRAVVSGLWKGVPLGE
IKVRLLLSNGEEYPHSGRIIFSDMAVDPNTDTIEMRALFPNPDQELLPGAYVRVVFDRAVRDNVFAIPRDAVIRTAQGAS
VFVVGPEGVLEARPVRADTLNGREWLVSEGLRDGDRVAVSHTMSLRPGMKVRSAAARPQPHAQQ
>Mature_384_residues
MKIINLLFCMPLLVVCFSCQREGESQAAGLPGETVPEVSVMTVRSTQVPVTANLPGRMEAYLQAEVRARVTGIIQERCYQ
EGQTVRPGDLLFKIDPAPLQAVLDECKAAVARARAVLSDAEDKAARYSSLVAKGAVSIREHKQARAEEERARAEYAAAAA
SLEQARLNLEYTRVEAPISGRVRRALVTEGAFANQNEFTHLTTIEQIDPIYVRFSQPASQYSSLRRAVVSGLWKGVPLGE
IKVRLLLSNGEEYPHSGRIIFSDMAVDPNTDTIEMRALFPNPDQELLPGAYVRVVFDRAVRDNVFAIPRDAVIRTAQGAS
VFVVGPEGVLEARPVRADTLNGREWLVSEGLRDGDRVAVSHTMSLRPGMKVRSAAARPQPHAQQ

Specific function: Part of a multidrug resistance efflux system that confers resistance to compounds such as rhodamine 6G, erythromycin, doxorubicin, ethidium bromide, TPP, SDS, deoxycholate, crystal violet and benzalkonium [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=375, Percent_Identity=38.4, Blast_Score=259, Evalue=2e-70,
Organism=Escherichia coli, GI1786668, Length=343, Percent_Identity=41.1078717201166, Blast_Score=258, Evalue=4e-70,
Organism=Escherichia coli, GI1789665, Length=379, Percent_Identity=39.8416886543536, Blast_Score=255, Evalue=4e-69,
Organism=Escherichia coli, GI87082036, Length=318, Percent_Identity=29.2452830188679, Blast_Score=117, Evalue=9e-28,
Organism=Escherichia coli, GI87081951, Length=132, Percent_Identity=31.8181818181818, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42155; Mature: 42155

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIINLLFCMPLLVVCFSCQREGESQAAGLPGETVPEVSVMTVRSTQVPVTANLPGRMEA
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEECCCCHHHH
YLQAEVRARVTGIIQERCYQEGQTVRPGDLLFKIDPAPLQAVLDECKAAVARARAVLSDA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCC
EDKAARYSSLVAKGAVSIREHKQARAEEERARAEYAAAAASLEQARLNLEYTRVEAPISG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCH
RVRRALVTEGAFANQNEFTHLTTIEQIDPIYVRFSQPASQYSSLRRAVVSGLWKGVPLGE
HHHHHHHHCCCCCCCCCCEEEEEHHHCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCC
IKVRLLLSNGEEYPHSGRIIFSDMAVDPNTDTIEMRALFPNPDQELLPGAYVRVVFDRAV
EEEEEEEECCCCCCCCCCEEEEEEECCCCCCEEEEEEECCCCCHHHCCCHHHHHHHHHHH
RDNVFAIPRDAVIRTAQGASVFVVGPEGVLEARPVRADTLNGREWLVSEGLRDGDRVAVS
HCCEEECCCHHHHEECCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCEEEEE
HTMSLRPGMKVRSAAARPQPHAQQ
EEEECCCCCHHHHHCCCCCCCCCC
>Mature Secondary Structure
MKIINLLFCMPLLVVCFSCQREGESQAAGLPGETVPEVSVMTVRSTQVPVTANLPGRMEA
CHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCCCCEEECCCCHHHH
YLQAEVRARVTGIIQERCYQEGQTVRPGDLLFKIDPAPLQAVLDECKAAVARARAVLSDA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHCC
EDKAARYSSLVAKGAVSIREHKQARAEEERARAEYAAAAASLEQARLNLEYTRVEAPISG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCH
RVRRALVTEGAFANQNEFTHLTTIEQIDPIYVRFSQPASQYSSLRRAVVSGLWKGVPLGE
HHHHHHHHCCCCCCCCCCEEEEEHHHCCCEEEEECCCHHHHHHHHHHHHHHHHCCCCCCC
IKVRLLLSNGEEYPHSGRIIFSDMAVDPNTDTIEMRALFPNPDQELLPGAYVRVVFDRAV
EEEEEEEECCCCCCCCCCEEEEEEECCCCCCEEEEEEECCCCCHHHCCCHHHHHHHHHHH
RDNVFAIPRDAVIRTAQGASVFVVGPEGVLEARPVRADTLNGREWLVSEGLRDGDRVAVS
HCCEEECCCHHHHEECCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCEEEEE
HTMSLRPGMKVRSAAARPQPHAQQ
EEEECCCCCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]