Click here to switch to the map view.

The map label for this gene is lipB [H]

Identifier: 187735380

GI number: 187735380

Start: 1049383

End: 1050168

Strand: Direct

Name: lipB [H]

Synonym: Amuc_0878

Alternate gene names: 187735380

Gene position: 1049383-1050168 (Clockwise)

Preceding gene: 187735378

Following gene: 187735381

Centisome position: 39.39

GC content: 61.7

Gene sequence:

>786_bases
ATGACCTCTGTGATGAACATTCTCTTTTTGGGCACAGGAACGTCCACCGGAGTGCCGCAGATAGGCTGCTCCTGCGCGGT
CTGCACGTCCCCGGACCCCAGAAACAGGCGGTTGCGTTCTTCAATTTATGTGGAGGCCGCTGGCACCCGCCTCCTGCTGG
ATTCTTCCCCGGATTTGCGGCAGCAGGCCCTGCGGGAAAATATCACGGATGTGGATGCCGTTCTTTATACGCATGCCCAT
GTGGACCACGTGGGAGGTTTTGACGATTTGCGCGCCTTCTGCTGGCGCCGGTCCGGCGGCCTGCCCATGTATGCTTCTCC
CATGACGGTGGACGCCCTGCGGACCATGTACGGCTGGGCGTTCGTTCCTAAACCGGGGCGGAGCGGGTACGTCAGGCCGG
AGCCTCACGAGGTGACGGCTCCGTTCCGTGTGGGGAACATGCTGGCGACGCCCCTTCCGGTGCTCCATGCCGGGGTGGAG
ACATATGCCTATCTCCTGGAGGCGGGAGGCCGGAGCCTGGTTTACATGCCGGATGTGAAAAGCATCCCGGCCCCGTCCCT
GGAGAGGATGAAAGGAGTGGATTTGCTGATTATTGACGGACTGCGTTATCATCTGCACCCCACGCACATGTGCCTGGAGG
AAACGTTGGCCGCCATTGCCGCCGTCCGGCCCCGCCGCGCCGTGCTGACCCACCTGTCCCATGATATGGATTACGGGATT
CTTTCCGGCAAGCTGCCGGAAAACGTCATGCCCGCGTATGACGGGCTGAGGCTGTCCCTGCCGTAA

Upstream 100 bases:

>100_bases
CAGCTGGTCCACAAGGGCCAGGTTTTTAATTTTCAGCAATGTAAGCATTCGGGCTTTGGATTTTGAATGCATTATGGCAT
CATTTGCCCTAAACGCAATC

Downstream 100 bases:

>100_bases
TGCTCACGCTGCATGTTCTCAGGCCATGACTATTCCAGACAATTTTGACCGTCCCGGCGCGCGGATACCGTGCGAAACCA
GAAACGTATTCGGCTTTTCC

Product: beta-lactamase domain protein

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 261; Mature: 260

Protein sequence:

>261_residues
MTSVMNILFLGTGTSTGVPQIGCSCAVCTSPDPRNRRLRSSIYVEAAGTRLLLDSSPDLRQQALRENITDVDAVLYTHAH
VDHVGGFDDLRAFCWRRSGGLPMYASPMTVDALRTMYGWAFVPKPGRSGYVRPEPHEVTAPFRVGNMLATPLPVLHAGVE
TYAYLLEAGGRSLVYMPDVKSIPAPSLERMKGVDLLIIDGLRYHLHPTHMCLEETLAAIAAVRPRRAVLTHLSHDMDYGI
LSGKLPENVMPAYDGLRLSLP

Sequences:

>Translated_261_residues
MTSVMNILFLGTGTSTGVPQIGCSCAVCTSPDPRNRRLRSSIYVEAAGTRLLLDSSPDLRQQALRENITDVDAVLYTHAH
VDHVGGFDDLRAFCWRRSGGLPMYASPMTVDALRTMYGWAFVPKPGRSGYVRPEPHEVTAPFRVGNMLATPLPVLHAGVE
TYAYLLEAGGRSLVYMPDVKSIPAPSLERMKGVDLLIIDGLRYHLHPTHMCLEETLAAIAAVRPRRAVLTHLSHDMDYGI
LSGKLPENVMPAYDGLRLSLP
>Mature_260_residues
TSVMNILFLGTGTSTGVPQIGCSCAVCTSPDPRNRRLRSSIYVEAAGTRLLLDSSPDLRQQALRENITDVDAVLYTHAHV
DHVGGFDDLRAFCWRRSGGLPMYASPMTVDALRTMYGWAFVPKPGRSGYVRPEPHEVTAPFRVGNMLATPLPVLHAGVET
YAYLLEAGGRSLVYMPDVKSIPAPSLERMKGVDLLIIDGLRYHLHPTHMCLEETLAAIAAVRPRRAVLTHLSHDMDYGIL
SGKLPENVMPAYDGLRLSLP

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG1235

COG function: function code R; Metal-dependent hydrolases of the beta-lactamase superfamily I

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the lipB family [H]

Homologues:

Organism=Escherichia coli, GI1790530, Length=256, Percent_Identity=28.125, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF00753 Lactamase_B [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 28638; Mature: 28506

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSVMNILFLGTGTSTGVPQIGCSCAVCTSPDPRNRRLRSSIYVEAAGTRLLLDSSPDLR
CCCCEEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHEEEEEECCCEEEECCCCHHH
QQALRENITDVDAVLYTHAHVDHVGGFDDLRAFCWRRSGGLPMYASPMTVDALRTMYGWA
HHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHCEE
FVPKPGRSGYVRPEPHEVTAPFRVGNMLATPLPVLHAGVETYAYLLEAGGRSLVYMPDVK
ECCCCCCCCCCCCCCCCCCCCCHHCCHHHCCHHHHHHHHHHHHHHHHCCCCEEEECCCCC
SIPAPSLERMKGVDLLIIDGLRYHLHPTHMCLEETLAAIAAVRPRRAVLTHLSHDMDYGI
CCCCCCHHHHCCCCEEEECCCEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCE
LSGKLPENVMPAYDGLRLSLP
ECCCCCCCCCCCCCCEEEECC
>Mature Secondary Structure 
TSVMNILFLGTGTSTGVPQIGCSCAVCTSPDPRNRRLRSSIYVEAAGTRLLLDSSPDLR
CCCEEEEEEECCCCCCCCCCCCEEEEECCCCHHHHHHHHEEEEEECCCEEEECCCCHHH
QQALRENITDVDAVLYTHAHVDHVGGFDDLRAFCWRRSGGLPMYASPMTVDALRTMYGWA
HHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHCEE
FVPKPGRSGYVRPEPHEVTAPFRVGNMLATPLPVLHAGVETYAYLLEAGGRSLVYMPDVK
ECCCCCCCCCCCCCCCCCCCCCHHCCHHHCCHHHHHHHHHHHHHHHHCCCCEEEECCCCC
SIPAPSLERMKGVDLLIIDGLRYHLHPTHMCLEETLAAIAAVRPRRAVLTHLSHDMDYGI
CCCCCCHHHHCCCCEEEECCCEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCE
LSGKLPENVMPAYDGLRLSLP
ECCCCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12949112 [H]