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The map label for this gene is clpB2 [H]

Identifier: 187735338

GI number: 187735338

Start: 986447

End: 989029

Strand: Direct

Name: clpB2 [H]

Synonym: Amuc_0836

Alternate gene names: 187735338

Gene position: 986447-989029 (Clockwise)

Preceding gene: 187735337

Following gene: 187735339

Centisome position: 37.03

GC content: 56.52

Gene sequence:

>2583_bases
ATGCTTAACAATCTAACAACAAAATTTCAGGAAGCGCTGATGCAGGCGCAGCAAACTGCAGGACGGCTTGGAAAACCGGA
AATCTCCTCTCTGGACGTTCTGGTGGCGTTGCTGGAGCAGGAAGGCGGAATTCTTTCCCCTATTCTCCGCAAGGCCTCTT
GCGACCCGGGGTTGTTTCTCCAGGCGGCCCAGAGGGAAGTTTCCCACGAACCTACCCAAAGCGGTGCCACCACCCAGCCC
CAGATGGGGCGTGACCTGGCTACGGTAATGCAGGCTGCGGAAGAAGAACGCAAAAAGCTCAAAGATGACTACCTCAGCGT
GGAACATTTCATGCTTGGTGCGTTGGACACGCAAAACAAAGTGCACCAGTTGACGGACACCTTTGGCCTGACCAAGGATA
ATTATCTGGCCGCCATGAAAGAAGTGCGCGGCAACCAGCGCGTGACGGACGATAATCCGGAAGGCAAATACCAGACGCTG
GAAAAATATGGAACGGACCTGACGGCCCGCGCCCGTGCCGGTAAAATTGACCCTGTTATCGGCCGCGATACGGAAATCCG
CCGTGTTTTGCAGATTCTTTCCCGCAGGACGAAAAACAATCCCGTCCTGATCGGGGAACCCGGCGTTGGTAAAACCGCCA
TTGCGGAAGGTTTGGCGCGGCGCATAGTCAACGGCGACGTGCCGGAAAGCATGCGCAACAAGCGCATTGTTTCGCTCAAC
ATCGGATCCATGCTCGCCGGGGCCAAATATCGTGGCGAATTTGAAGAGCGCTTGAAATCCTTCCTTAAGGAAGTAACGGA
TTCCAACGGTGAAATCATTCTATTTATTGATGAACTGCACACCATCGTAGGCGCGGGCGCCAGTGAAGGGGCGGTGGATG
CGTCCAACCTGCTCAAACCCGCCCTGGCCCGCGGGGAACTGCGGACCATCGGCGCGACGACACTGGACGAATACCGCAAA
TACATTGAAAAGGATGCGGCTCTGGAACGCCGGTTTCAGCCCGTCATGGTCTCCGAGCCCAGCGTGGAAGATACCATTGC
CATCCTGCGCGGCCTGAAAGAACGCTATGAAGTTCACCATGGGGTGCGTATTACGGACGCTGCCATCGTGGCGGCCGCCA
CCCTTTCCGACCGTTATATTTCCGACCGTTTTCTGCCTGACAAGGCAGTGGATCTGGTGGATGAAGCGGCAGCCCGCCTC
AAAATTGAGCTGGACTCCATGCCCACGGAAATTGACCAGATTGAACGTGAAGCCATGCAGCTTGAAATGGAGCGGCAGGC
GCTGGCGAAGGAAGAAGACGCCGACAGCAAGGCGCGTTTGGAAAAAATCACCAAGGACCTGGCCGATTTGAAGGAAAAAT
CCGGTTCCATGATTGCCAAATGGAAAAGTGAAAAGGAAGTGCTGGACGCCGTCCGCAGGGAACAGGAAAAAATAGAAGCC
CTCAAGCTGGAAAGTGAACGGGCCAAACGGATGGGTGATCTGACCCGCGCATCGGAAATCACCTATGGCGAACTTCCGGA
GGCCCAGCGCGCCCTGGCGGAAGGCAAGGAAAAGCTCAGTAAAATGCAGAAGGAAGATGGAGGGCTGCTGAAGGAGGAAG
TCACGGAAGGAGACATCGCCAAAGTGGTTGCCACCTGGACCGGCATCCCCGCCGCCCGTCTGCAGGAAGGGGAGCGTGCC
AAGCTGGTGCACATGGAAGAACGTCTGGGCGCCCGCGTAATCGGGCAGAAACAGGCCGTTAAGGCCGTATCCGACGCCGT
GAGGCGCGCCCGCGCCGGGTTGCAGGATGAAAACAGGCCCATTGGCTCTTTCCTGTTCCTGGGACCCACCGGCGTCGGCA
AGACGGAGCTCAGCAAGGCACTGGCGGAATTCCTTTTTGACAATGAAAACGCCATCGTCCGCATTGACATGTCCGAATAC
ATGGAAAAACATTCTGTTGCGCGCCTCATCGGGGCGCCTCCCGGATACGTGGGGTATGAAGAAGGCGGTCAGCTCTCTGA
GGCCGTGCGGCGCCGTCCGTACTGCGTGGTTCTCTTCGATGAAATTGAAAAGGCCCACCCGGACGTCTTCAACGTATTGC
TCCAGGTATTGGACGACGGCCGTATTACGGACGGCCAGGGGCGTACGGTTGACTTCCGCAACACGGTCATCATCATGACC
TCCAACATCGGCAGCCAGTATATCCTCAATGAAGCCAATGCGGAACAGCGGGAAGCCAAGGCCCTGGAGGCGTTGCGAGG
CCACTTCCGTCCGGAATTCCTGAACCGCATTGATGAAATCATCATCTTTGACCGGCTCACGGCGCAGGAACTCAAGGGCA
TTGTGGACATTCAGCTTCAGCGTGTCCGCAGGCGCCTGGAAGCCAAAGGCCTGTTTTTGCGCATGACTCCGGAAGCTACG
GCTCTGGTGGCGGATCACGGTTTTGATCCTGTTTATGGAGCCCGGCCTCTCAAGCGGGCCATCCAGCATGATCTTCTGGA
CCCGCTCAGCCTTAAACTTCTGGAAGGGGACTTCCCGGAAGGAACGGAAATCGTGGTGCGGGAAAAAGGCGGCAAATTGG
ACTTCACCAAGGAGAAAAAATAA

Upstream 100 bases:

>100_bases
GGGGAATCCGGAGGGGCAAAAAATTTTTAGATAACAGGAAAAATCCTGTTTTTCCATTCAACGGTTGGAGTTATTCTTAT
GTCTAAAATACCAACATACC

Downstream 100 bases:

>100_bases
CTGAAAAGCATGCTTGCCGGCGTGTTGCCGGGAGCGGACAGGGGCGCGGCGTTAGCTGCGTCCCTGTTTATTGCTCCAAT
GGTGCGGAAAGGCCGCGTAT

Product: ATP-dependent chaperone ClpB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 860; Mature: 860

Protein sequence:

>860_residues
MLNNLTTKFQEALMQAQQTAGRLGKPEISSLDVLVALLEQEGGILSPILRKASCDPGLFLQAAQREVSHEPTQSGATTQP
QMGRDLATVMQAAEEERKKLKDDYLSVEHFMLGALDTQNKVHQLTDTFGLTKDNYLAAMKEVRGNQRVTDDNPEGKYQTL
EKYGTDLTARARAGKIDPVIGRDTEIRRVLQILSRRTKNNPVLIGEPGVGKTAIAEGLARRIVNGDVPESMRNKRIVSLN
IGSMLAGAKYRGEFEERLKSFLKEVTDSNGEIILFIDELHTIVGAGASEGAVDASNLLKPALARGELRTIGATTLDEYRK
YIEKDAALERRFQPVMVSEPSVEDTIAILRGLKERYEVHHGVRITDAAIVAAATLSDRYISDRFLPDKAVDLVDEAAARL
KIELDSMPTEIDQIEREAMQLEMERQALAKEEDADSKARLEKITKDLADLKEKSGSMIAKWKSEKEVLDAVRREQEKIEA
LKLESERAKRMGDLTRASEITYGELPEAQRALAEGKEKLSKMQKEDGGLLKEEVTEGDIAKVVATWTGIPAARLQEGERA
KLVHMEERLGARVIGQKQAVKAVSDAVRRARAGLQDENRPIGSFLFLGPTGVGKTELSKALAEFLFDNENAIVRIDMSEY
MEKHSVARLIGAPPGYVGYEEGGQLSEAVRRRPYCVVLFDEIEKAHPDVFNVLLQVLDDGRITDGQGRTVDFRNTVIIMT
SNIGSQYILNEANAEQREAKALEALRGHFRPEFLNRIDEIIIFDRLTAQELKGIVDIQLQRVRRRLEAKGLFLRMTPEAT
ALVADHGFDPVYGARPLKRAIQHDLLDPLSLKLLEGDFPEGTEIVVREKGGKLDFTKEKK

Sequences:

>Translated_860_residues
MLNNLTTKFQEALMQAQQTAGRLGKPEISSLDVLVALLEQEGGILSPILRKASCDPGLFLQAAQREVSHEPTQSGATTQP
QMGRDLATVMQAAEEERKKLKDDYLSVEHFMLGALDTQNKVHQLTDTFGLTKDNYLAAMKEVRGNQRVTDDNPEGKYQTL
EKYGTDLTARARAGKIDPVIGRDTEIRRVLQILSRRTKNNPVLIGEPGVGKTAIAEGLARRIVNGDVPESMRNKRIVSLN
IGSMLAGAKYRGEFEERLKSFLKEVTDSNGEIILFIDELHTIVGAGASEGAVDASNLLKPALARGELRTIGATTLDEYRK
YIEKDAALERRFQPVMVSEPSVEDTIAILRGLKERYEVHHGVRITDAAIVAAATLSDRYISDRFLPDKAVDLVDEAAARL
KIELDSMPTEIDQIEREAMQLEMERQALAKEEDADSKARLEKITKDLADLKEKSGSMIAKWKSEKEVLDAVRREQEKIEA
LKLESERAKRMGDLTRASEITYGELPEAQRALAEGKEKLSKMQKEDGGLLKEEVTEGDIAKVVATWTGIPAARLQEGERA
KLVHMEERLGARVIGQKQAVKAVSDAVRRARAGLQDENRPIGSFLFLGPTGVGKTELSKALAEFLFDNENAIVRIDMSEY
MEKHSVARLIGAPPGYVGYEEGGQLSEAVRRRPYCVVLFDEIEKAHPDVFNVLLQVLDDGRITDGQGRTVDFRNTVIIMT
SNIGSQYILNEANAEQREAKALEALRGHFRPEFLNRIDEIIIFDRLTAQELKGIVDIQLQRVRRRLEAKGLFLRMTPEAT
ALVADHGFDPVYGARPLKRAIQHDLLDPLSLKLLEGDFPEGTEIVVREKGGKLDFTKEKK
>Mature_860_residues
MLNNLTTKFQEALMQAQQTAGRLGKPEISSLDVLVALLEQEGGILSPILRKASCDPGLFLQAAQREVSHEPTQSGATTQP
QMGRDLATVMQAAEEERKKLKDDYLSVEHFMLGALDTQNKVHQLTDTFGLTKDNYLAAMKEVRGNQRVTDDNPEGKYQTL
EKYGTDLTARARAGKIDPVIGRDTEIRRVLQILSRRTKNNPVLIGEPGVGKTAIAEGLARRIVNGDVPESMRNKRIVSLN
IGSMLAGAKYRGEFEERLKSFLKEVTDSNGEIILFIDELHTIVGAGASEGAVDASNLLKPALARGELRTIGATTLDEYRK
YIEKDAALERRFQPVMVSEPSVEDTIAILRGLKERYEVHHGVRITDAAIVAAATLSDRYISDRFLPDKAVDLVDEAAARL
KIELDSMPTEIDQIEREAMQLEMERQALAKEEDADSKARLEKITKDLADLKEKSGSMIAKWKSEKEVLDAVRREQEKIEA
LKLESERAKRMGDLTRASEITYGELPEAQRALAEGKEKLSKMQKEDGGLLKEEVTEGDIAKVVATWTGIPAARLQEGERA
KLVHMEERLGARVIGQKQAVKAVSDAVRRARAGLQDENRPIGSFLFLGPTGVGKTELSKALAEFLFDNENAIVRIDMSEY
MEKHSVARLIGAPPGYVGYEEGGQLSEAVRRRPYCVVLFDEIEKAHPDVFNVLLQVLDDGRITDGQGRTVDFRNTVIIMT
SNIGSQYILNEANAEQREAKALEALRGHFRPEFLNRIDEIIIFDRLTAQELKGIVDIQLQRVRRRLEAKGLFLRMTPEAT
ALVADHGFDPVYGARPLKRAIQHDLLDPLSLKLLEGDFPEGTEIVVREKGGKLDFTKEKK

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=314, Percent_Identity=36.3057324840764, Blast_Score=211, Evalue=3e-54,
Organism=Escherichia coli, GI1788943, Length=846, Percent_Identity=54.8463356973995, Blast_Score=936, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=250, Percent_Identity=56.4, Blast_Score=289, Evalue=6e-79,
Organism=Saccharomyces cerevisiae, GI6320464, Length=714, Percent_Identity=53.0812324929972, Blast_Score=759, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323002, Length=870, Percent_Identity=41.2643678160919, Blast_Score=661, Evalue=0.0,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 95759; Mature: 95759

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00870 CLPAB_1 ; PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNNLTTKFQEALMQAQQTAGRLGKPEISSLDVLVALLEQEGGILSPILRKASCDPGLFL
CCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHH
QAAQREVSHEPTQSGATTQPQMGRDLATVMQAAEEERKKLKDDYLSVEHFMLGALDTQNK
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
VHQLTDTFGLTKDNYLAAMKEVRGNQRVTDDNPEGKYQTLEKYGTDLTARARAGKIDPVI
HHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHCCCCCCCCCC
GRDTEIRRVLQILSRRTKNNPVLIGEPGVGKTAIAEGLARRIVNGDVPESMRNKRIVSLN
CCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHCCEEEEEE
IGSMLAGAKYRGEFEERLKSFLKEVTDSNGEIILFIDELHTIVGAGASEGAVDASNLLKP
HHHHHHCCHHCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHH
ALARGELRTIGATTLDEYRKYIEKDAALERRFQPVMVSEPSVEDTIAILRGLKERYEVHH
HHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHC
GVRITDAAIVAAATLSDRYISDRFLPDKAVDLVDEAAARLKIELDSMPTEIDQIEREAMQ
CCEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHH
LEMERQALAKEEDADSKARLEKITKDLADLKEKSGSMIAKWKSEKEVLDAVRREQEKIEA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHCHHHHHHHHHHHHHHHHHH
LKLESERAKRMGDLTRASEITYGELPEAQRALAEGKEKLSKMQKEDGGLLKEEVTEGDIA
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHH
KVVATWTGIPAARLQEGERAKLVHMEERLGARVIGQKQAVKAVSDAVRRARAGLQDENRP
HHHHHHCCCCHHHHCCCCCHHHEEHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
IGSFLFLGPTGVGKTELSKALAEFLFDNENAIVRIDMSEYMEKHSVARLIGAPPGYVGYE
CCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCC
EGGQLSEAVRRRPYCVVLFDEIEKAHPDVFNVLLQVLDDGRITDGQGRTVDFRNTVIIMT
CCCHHHHHHHHCCEEEEEEHHHHHHCCHHHHHHHHHHCCCCEECCCCCEEEECCEEEEEE
SNIGSQYILNEANAEQREAKALEALRGHFRPEFLNRIDEIIIFDRLTAQELKGIVDIQLQ
CCCCCCHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVRRRLEAKGLFLRMTPEATALVADHGFDPVYGARPLKRAIQHDLLDPLSLKLLEGDFPE
HHHHHHHHCCEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCC
GTEIVVREKGGKLDFTKEKK
CCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure
MLNNLTTKFQEALMQAQQTAGRLGKPEISSLDVLVALLEQEGGILSPILRKASCDPGLFL
CCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCHHH
QAAQREVSHEPTQSGATTQPQMGRDLATVMQAAEEERKKLKDDYLSVEHFMLGALDTQNK
HHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
VHQLTDTFGLTKDNYLAAMKEVRGNQRVTDDNPEGKYQTLEKYGTDLTARARAGKIDPVI
HHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCHHCCCCCCCCCC
GRDTEIRRVLQILSRRTKNNPVLIGEPGVGKTAIAEGLARRIVNGDVPESMRNKRIVSLN
CCCHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCHHHHCCEEEEEE
IGSMLAGAKYRGEFEERLKSFLKEVTDSNGEIILFIDELHTIVGAGASEGAVDASNLLKP
HHHHHHCCHHCCHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHCCCCCCCCCCHHHHHHH
ALARGELRTIGATTLDEYRKYIEKDAALERRFQPVMVSEPSVEDTIAILRGLKERYEVHH
HHHCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHHHHHHHC
GVRITDAAIVAAATLSDRYISDRFLPDKAVDLVDEAAARLKIELDSMPTEIDQIEREAMQ
CCEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHH
LEMERQALAKEEDADSKARLEKITKDLADLKEKSGSMIAKWKSEKEVLDAVRREQEKIEA
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHCHHHHHHHHHHHHHHHHHH
LKLESERAKRMGDLTRASEITYGELPEAQRALAEGKEKLSKMQKEDGGLLKEEVTEGDIA
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCHHH
KVVATWTGIPAARLQEGERAKLVHMEERLGARVIGQKQAVKAVSDAVRRARAGLQDENRP
HHHHHHCCCCHHHHCCCCCHHHEEHHHHHCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
IGSFLFLGPTGVGKTELSKALAEFLFDNENAIVRIDMSEYMEKHSVARLIGAPPGYVGYE
CCCEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCCCC
EGGQLSEAVRRRPYCVVLFDEIEKAHPDVFNVLLQVLDDGRITDGQGRTVDFRNTVIIMT
CCCHHHHHHHHCCEEEEEEHHHHHHCCHHHHHHHHHHCCCCEECCCCCEEEECCEEEEEE
SNIGSQYILNEANAEQREAKALEALRGHFRPEFLNRIDEIIIFDRLTAQELKGIVDIQLQ
CCCCCCHHCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVRRRLEAKGLFLRMTPEATALVADHGFDPVYGARPLKRAIQHDLLDPLSLKLLEGDFPE
HHHHHHHHCCEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCC
GTEIVVREKGGKLDFTKEKK
CCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]