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The map label for this gene is lysS [H]

Identifier: 187735301

GI number: 187735301

Start: 935258

End: 936739

Strand: Reverse

Name: lysS [H]

Synonym: Amuc_0798

Alternate gene names: 187735301

Gene position: 936739-935258 (Counterclockwise)

Preceding gene: 187735303

Following gene: 187735300

Centisome position: 35.16

GC content: 57.15

Gene sequence:

>1482_bases
ATGTCCGAACAACAGCAGGCACATCCGACTACGACGGAATCCGAACTTATTGCCGTGCGCCGCGACAAGCTCGCCAAAAT
CCGTGAGCTGGGAATTGACCCCTACGGCGCAAGATTTGACGTGACCACCACTCCCGCCGGATTGAAAGCCGATTTCCAGG
AAGACAAACAGGTAGCCGTCGCCGGCCGCCTTCTGGCTATTCGCGACATGGGCAAATCCCAGTTTTTCGTCATTGGGGAC
GTACGGGGAAAAATCCAGGGGTTCCTGCATAAAAATGAGGTGGATGAAACCACCTGGAAGCTTTGGAAGCTCCTGGACCG
CGGGGACTGGGTCGGCATCAGGGGAACCACGTTCCTGACCCGTACCGGAGAACCTACCGTAAAGGTATCCGGACTGACCA
TTCTTTCCAAAAGCCTCCGCCCCCTGCCGGACAAGTGGCACGGGCTGGCGGACAAGGAGGTGACCTACCGCAAGCGCCAC
CTGGACCTCATTTCCAATGAGGAAAGCGCCTCCCTGTTCGTTACGCGCTCCCTCATGATTGCGGAAATCCGCCGCTTTCT
CCAGGAACGCGGTTATCTGGAAGTGGAAACCCCCATGCTTCAGGATGTGGCCGGTGGGGCCGCCGCCAAGCCGTTTGAAA
CGTACCATAACGCGCTGGATATGCCGCTGACGCTGCGCATTGCCCCGGAGCTTTTCCTCAAACGCCTGATGGTGGGCGGC
TTCACGAAAATTTTCGAACTCAACCGCAGCTTCCGCAATGAAGGAATTGACCGCCGCCACAACCCGGAATTCACCATGCT
GGAAGCCTATTGCGCCTGCGGTGATTTTGAAACCATGGCGAATATGGTGGAGGAGCTCATCTGCCATCTGGCGGAAAAAT
TCTGCGGTGGCCTTCAGATTGACCACAAGGACGCGGAAGGCAACGTTCTTTACACAATCGACCTCAGCCGGCCCTGGAGA
CGCGCCGACTACCAGGACCTGATCCGGGGCGTGGCGGGGGAAGACTGGTTTGACATCTCCCCCGAAGCGCGCCGCGCCCG
CTGTGAAGAGCTGGGAGTGGAAATCAGCCCGGATATGAAAGATGTGGATGTTTCCCAGCAGGTGTATGAAAAACTGGTGG
AGGAAAAGACCATGAACCCCTGCTTTGTTACCCACGTGGCCAAGGACTTGGTTCCCCTGGCCAAGCTGAACCGGGAAAAT
CCGGACGTAGTGGATGTATATGAACTGGTGATCAACGGGCAGGAAATTTCCCCCGGCTATTCGGAATTGAACGATCCTGA
CGTGCAAAAGGAACGCCTGGAGCACCAGGCTGCCGGGGAGACCCAGCGTGTGGACTATGATTTCATTGAAACGCTGGAAT
ACGGAATGCCCTCCGCAGGCGGCATCGGCATCGGCATCGACCGCGTCGTCATGATGCTGACGGGGGCCTCTTCCATCCGT
GACGTGCTGCTCTTCCCGCAGTTGAAACGTAAGGACAGTTAA

Upstream 100 bases:

>100_bases
GGGGGAGGAACCTTCGGGTCAAAACCGGCCGCCAAAAATGTCCCCTCTGCCTGACAATGCGCTAGCCAAAACGGAAGCAA
GCCTTTAGAATCCCCGCACT

Downstream 100 bases:

>100_bases
TCCGAACCACCGGCCGCATGGCTCCCCGCACCCCGGATATTCTGGACTGCGCTTCCGCCCAATGGATCGGCAAACGCCGC
GAGCAGGAAGACGTGGTTAA

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSEQQQAHPTTTESELIAVRRDKLAKIRELGIDPYGARFDVTTTPAGLKADFQEDKQVAVAGRLLAIRDMGKSQFFVIGD
VRGKIQGFLHKNEVDETTWKLWKLLDRGDWVGIRGTTFLTRTGEPTVKVSGLTILSKSLRPLPDKWHGLADKEVTYRKRH
LDLISNEESASLFVTRSLMIAEIRRFLQERGYLEVETPMLQDVAGGAAAKPFETYHNALDMPLTLRIAPELFLKRLMVGG
FTKIFELNRSFRNEGIDRRHNPEFTMLEAYCACGDFETMANMVEELICHLAEKFCGGLQIDHKDAEGNVLYTIDLSRPWR
RADYQDLIRGVAGEDWFDISPEARRARCEELGVEISPDMKDVDVSQQVYEKLVEEKTMNPCFVTHVAKDLVPLAKLNREN
PDVVDVYELVINGQEISPGYSELNDPDVQKERLEHQAAGETQRVDYDFIETLEYGMPSAGGIGIGIDRVVMMLTGASSIR
DVLLFPQLKRKDS

Sequences:

>Translated_493_residues
MSEQQQAHPTTTESELIAVRRDKLAKIRELGIDPYGARFDVTTTPAGLKADFQEDKQVAVAGRLLAIRDMGKSQFFVIGD
VRGKIQGFLHKNEVDETTWKLWKLLDRGDWVGIRGTTFLTRTGEPTVKVSGLTILSKSLRPLPDKWHGLADKEVTYRKRH
LDLISNEESASLFVTRSLMIAEIRRFLQERGYLEVETPMLQDVAGGAAAKPFETYHNALDMPLTLRIAPELFLKRLMVGG
FTKIFELNRSFRNEGIDRRHNPEFTMLEAYCACGDFETMANMVEELICHLAEKFCGGLQIDHKDAEGNVLYTIDLSRPWR
RADYQDLIRGVAGEDWFDISPEARRARCEELGVEISPDMKDVDVSQQVYEKLVEEKTMNPCFVTHVAKDLVPLAKLNREN
PDVVDVYELVINGQEISPGYSELNDPDVQKERLEHQAAGETQRVDYDFIETLEYGMPSAGGIGIGIDRVVMMLTGASSIR
DVLLFPQLKRKDS
>Mature_492_residues
SEQQQAHPTTTESELIAVRRDKLAKIRELGIDPYGARFDVTTTPAGLKADFQEDKQVAVAGRLLAIRDMGKSQFFVIGDV
RGKIQGFLHKNEVDETTWKLWKLLDRGDWVGIRGTTFLTRTGEPTVKVSGLTILSKSLRPLPDKWHGLADKEVTYRKRHL
DLISNEESASLFVTRSLMIAEIRRFLQERGYLEVETPMLQDVAGGAAAKPFETYHNALDMPLTLRIAPELFLKRLMVGGF
TKIFELNRSFRNEGIDRRHNPEFTMLEAYCACGDFETMANMVEELICHLAEKFCGGLQIDHKDAEGNVLYTIDLSRPWRR
ADYQDLIRGVAGEDWFDISPEARRARCEELGVEISPDMKDVDVSQQVYEKLVEEKTMNPCFVTHVAKDLVPLAKLNRENP
DVVDVYELVINGQEISPGYSELNDPDVQKERLEHQAAGETQRVDYDFIETLEYGMPSAGGIGIGIDRVVMMLTGASSIRD
VLLFPQLKRKDS

Specific function: Unknown

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=507, Percent_Identity=35.1084812623274, Blast_Score=294, Evalue=1e-79,
Organism=Homo sapiens, GI194272210, Length=507, Percent_Identity=35.1084812623274, Blast_Score=294, Evalue=1e-79,
Organism=Homo sapiens, GI45439306, Length=332, Percent_Identity=23.7951807228916, Blast_Score=77, Evalue=5e-14,
Organism=Escherichia coli, GI1789256, Length=508, Percent_Identity=40.748031496063, Blast_Score=387, Evalue=1e-109,
Organism=Escherichia coli, GI1790571, Length=508, Percent_Identity=40.1574803149606, Blast_Score=386, Evalue=1e-108,
Organism=Escherichia coli, GI87082379, Length=348, Percent_Identity=32.183908045977, Blast_Score=141, Evalue=1e-34,
Organism=Escherichia coli, GI1788173, Length=297, Percent_Identity=27.2727272727273, Blast_Score=79, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17535925, Length=517, Percent_Identity=35.7833655705996, Blast_Score=290, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI17535927, Length=517, Percent_Identity=35.7833655705996, Blast_Score=290, Evalue=1e-78,
Organism=Caenorhabditis elegans, GI71994340, Length=494, Percent_Identity=36.6396761133603, Blast_Score=290, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI32566633, Length=332, Percent_Identity=25.9036144578313, Blast_Score=80, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320242, Length=515, Percent_Identity=36.8932038834951, Blast_Score=299, Evalue=7e-82,
Organism=Saccharomyces cerevisiae, GI6324256, Length=530, Percent_Identity=28.4905660377358, Blast_Score=162, Evalue=8e-41,
Organism=Saccharomyces cerevisiae, GI6323011, Length=472, Percent_Identity=21.6101694915254, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI24640849, Length=495, Percent_Identity=36.969696969697, Blast_Score=305, Evalue=5e-83,
Organism=Drosophila melanogaster, GI24640851, Length=495, Percent_Identity=36.969696969697, Blast_Score=305, Evalue=6e-83,

Paralogues:

None

Copy number: 1200 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 55829; Mature: 55698

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEQQQAHPTTTESELIAVRRDKLAKIRELGIDPYGARFDVTTTPAGLKADFQEDKQVAV
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHCCHHHH
AGRLLAIRDMGKSQFFVIGDVRGKIQGFLHKNEVDETTWKLWKLLDRGDWVGIRGTTFLT
HHHHHHEECCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCCEEEE
RTGEPTVKVSGLTILSKSLRPLPDKWHGLADKEVTYRKRHLDLISNEESASLFVTRSLMI
ECCCCEEEECCHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCEEHHHHHHHH
AEIRRFLQERGYLEVETPMLQDVAGGAAAKPFETYHNALDMPLTLRIAPELFLKRLMVGG
HHHHHHHHHCCCEEECCHHHHHHCCCCCCCCHHHHHHHCCCCEEEEECHHHHHHHHHHCC
FTKIFELNRSFRNEGIDRRHNPEFTMLEAYCACGDFETMANMVEELICHLAEKFCGGLQI
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEE
DHKDAEGNVLYTIDLSRPWRRADYQDLIRGVAGEDWFDISPEARRARCEELGVEISPDMK
ECCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
DVDVSQQVYEKLVEEKTMNPCFVTHVAKDLVPLAKLNRENPDVVDVYELVINGQEISPGY
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCH
SELNDPDVQKERLEHQAAGETQRVDYDFIETLEYGMPSAGGIGIGIDRVVMMLTGASSIR
HHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCHHHH
DVLLFPQLKRKDS
HHHHCCCHHCCCC
>Mature Secondary Structure 
SEQQQAHPTTTESELIAVRRDKLAKIRELGIDPYGARFDVTTTPAGLKADFQEDKQVAV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCHHCCHHHH
AGRLLAIRDMGKSQFFVIGDVRGKIQGFLHKNEVDETTWKLWKLLDRGDWVGIRGTTFLT
HHHHHHEECCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEECCCEEEE
RTGEPTVKVSGLTILSKSLRPLPDKWHGLADKEVTYRKRHLDLISNEESASLFVTRSLMI
ECCCCEEEECCHHHHHHHCCCCCHHHCCCCCCHHHHHHHHHHHHCCCCCCEEHHHHHHHH
AEIRRFLQERGYLEVETPMLQDVAGGAAAKPFETYHNALDMPLTLRIAPELFLKRLMVGG
HHHHHHHHHCCCEEECCHHHHHHCCCCCCCCHHHHHHHCCCCEEEEECHHHHHHHHHHCC
FTKIFELNRSFRNEGIDRRHNPEFTMLEAYCACGDFETMANMVEELICHLAEKFCGGLQI
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCEE
DHKDAEGNVLYTIDLSRPWRRADYQDLIRGVAGEDWFDISPEARRARCEELGVEISPDMK
ECCCCCCCEEEEEECCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCCCC
DVDVSQQVYEKLVEEKTMNPCFVTHVAKDLVPLAKLNRENPDVVDVYELVINGQEISPGY
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCH
SELNDPDVQKERLEHQAAGETQRVDYDFIETLEYGMPSAGGIGIGIDRVVMMLTGASSIR
HHCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCHHHH
DVLLFPQLKRKDS
HHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA