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The map label for this gene is hsrA [H]

Identifier: 187735278

GI number: 187735278

Start: 911990

End: 913399

Strand: Direct

Name: hsrA [H]

Synonym: Amuc_0774

Alternate gene names: 187735278

Gene position: 911990-913399 (Clockwise)

Preceding gene: 187735277

Following gene: 187735280

Centisome position: 34.23

GC content: 58.79

Gene sequence:

>1410_bases
ATGGAAAAACTGCCGACATCCTCCAAAGCTCTGCGCTACCTGCCCTTTCTTGTGGCATCTGCATTCTTTATGCAGATGCT
GGATGCCACCATCCTGAACACGGCCATTCCCACCATTGCCCGCAGCTTCAACAGCCATCCGCTGCAGCTCCACTCCCTGG
TAACGGCCTACATGCTCACCGTCTGCGTCCTGATACCGGCCTCCGGATGGATATCCGACAAACTGGGTTCCCGCCGTACT
TTCCTGCTGGCCATCTCCGTTTTTTCCGTGGGCTCCCTGCTGTGCGCCCTTTCCGCCTCCGTTACCATGATGACGGTATG
CCGCGTCATCCAGGGAATAGGCGGCGCCTTCCTGATGCCCGTCGGGCGCCTTGTCATCCTGCGCTCCTATCCCCGCTCCA
TGTTCGTCAATGTCCTGAACATTGTAACGATTCCGGCGCTGCTGGGCCCCCTTCTGGGGCCGGTGCTGGGCGGTATCATC
GTACAGTACGCTTCCTGGCACTGGATATTCCTGATCAACATCCCGGTAGGCATGGCGGGCCTGTGGGCCACCTGGAAGCT
GATGCCGGACCTGAAAGCGGTCCGGGAACAGAAATTCGACTGGCCGGGTTTCTTCCTTTTCTCCTCCTCCGCCGTCCTGG
CTACGATGGGCCTCTCTTCCGCAGGAGGCATAACCGATAAGCCGCGCATGGTCATCCTCACGTCTGCCGGAGGCATTCTT
CAACTTCTGTACTGGGTGCTGGCGTTCCGTTCAAAAGCCCCCATCTTCAGCCCGGCCCTGTTCCGCATCAGAAACTTTGC
CATCGGAATCGCCGGCAACATCGTCTGCCGCCTGGGCGGAAGCTGCCTGCCGTACCTGATGCCGCTTTTCTTCCAGGTGG
TGCTTGGTTATTCCGCTCTTCAGTCCGGCATGTCCCTGATTCCGCTGGCCCTGAGCAATCTGCTGGCAAAAACGGTTGCG
CCGCGTCTGCTCGGGAAATTTGGTTACCGGAACATCATGGTCGTCAATACCTTCACCATAGGATCGCTGCTGGCCTCCTT
CCGCTTTGTAGGCCCCGGAACCCATGAACTTATTCTCCTGGGCATGCTCGCGTTGCTGGGCGGGGCCAACTCCATTCAGT
TCACCTGCATGAACACCCTGACGCTGATTGACCTCCCCAATGCAGACGCCAGCAGCGGAAACTCCCTGCTTTCCACGATC
ATGCAGCTCTCCATTGCTGTCAGTATTGCCGCGGCCTCCCTGCTGCTGGACAGCTTCGGCGGCCATTCGGCTACCTCCGG
AAAGGCTGTGGAAACCGCCTTTCATGCCACTTTCGCCACCATCGGCGCCATTGCCGCGGCCAGTTCCTTCATCTTCGCCC
TGGTGGACAAAGACAAGGGAATCACCCGCAAACGGAGAAAAGCAGCTTGA

Upstream 100 bases:

>100_bases
GCGTGCTGAAGCGGCGTCCTCCGGAACCCGGGGCGTAAAGCGGAACAAACATCTGCCTTGCCCCTGGCGGGGCCAGGCCG
GATACTGTCGGCGGTCTTTC

Downstream 100 bases:

>100_bases
AGAAGAAAATTCCCGCCCCTGTTCAGGAACAGGAAGAGGAATCTCCCGTCTCAAGAGAACAGCACTGCGCAAACCGGGCG
CGGGACTCTTCCAGGGTAAT

Product: major facilitator superfamily MFS_1

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MEKLPTSSKALRYLPFLVASAFFMQMLDATILNTAIPTIARSFNSHPLQLHSLVTAYMLTVCVLIPASGWISDKLGSRRT
FLLAISVFSVGSLLCALSASVTMMTVCRVIQGIGGAFLMPVGRLVILRSYPRSMFVNVLNIVTIPALLGPLLGPVLGGII
VQYASWHWIFLINIPVGMAGLWATWKLMPDLKAVREQKFDWPGFFLFSSSAVLATMGLSSAGGITDKPRMVILTSAGGIL
QLLYWVLAFRSKAPIFSPALFRIRNFAIGIAGNIVCRLGGSCLPYLMPLFFQVVLGYSALQSGMSLIPLALSNLLAKTVA
PRLLGKFGYRNIMVVNTFTIGSLLASFRFVGPGTHELILLGMLALLGGANSIQFTCMNTLTLIDLPNADASSGNSLLSTI
MQLSIAVSIAAASLLLDSFGGHSATSGKAVETAFHATFATIGAIAAASSFIFALVDKDKGITRKRRKAA

Sequences:

>Translated_469_residues
MEKLPTSSKALRYLPFLVASAFFMQMLDATILNTAIPTIARSFNSHPLQLHSLVTAYMLTVCVLIPASGWISDKLGSRRT
FLLAISVFSVGSLLCALSASVTMMTVCRVIQGIGGAFLMPVGRLVILRSYPRSMFVNVLNIVTIPALLGPLLGPVLGGII
VQYASWHWIFLINIPVGMAGLWATWKLMPDLKAVREQKFDWPGFFLFSSSAVLATMGLSSAGGITDKPRMVILTSAGGIL
QLLYWVLAFRSKAPIFSPALFRIRNFAIGIAGNIVCRLGGSCLPYLMPLFFQVVLGYSALQSGMSLIPLALSNLLAKTVA
PRLLGKFGYRNIMVVNTFTIGSLLASFRFVGPGTHELILLGMLALLGGANSIQFTCMNTLTLIDLPNADASSGNSLLSTI
MQLSIAVSIAAASLLLDSFGGHSATSGKAVETAFHATFATIGAIAAASSFIFALVDKDKGITRKRRKAA
>Mature_469_residues
MEKLPTSSKALRYLPFLVASAFFMQMLDATILNTAIPTIARSFNSHPLQLHSLVTAYMLTVCVLIPASGWISDKLGSRRT
FLLAISVFSVGSLLCALSASVTMMTVCRVIQGIGGAFLMPVGRLVILRSYPRSMFVNVLNIVTIPALLGPLLGPVLGGII
VQYASWHWIFLINIPVGMAGLWATWKLMPDLKAVREQKFDWPGFFLFSSSAVLATMGLSSAGGITDKPRMVILTSAGGIL
QLLYWVLAFRSKAPIFSPALFRIRNFAIGIAGNIVCRLGGSCLPYLMPLFFQVVLGYSALQSGMSLIPLALSNLLAKTVA
PRLLGKFGYRNIMVVNTFTIGSLLASFRFVGPGTHELILLGMLALLGGANSIQFTCMNTLTLIDLPNADASSGNSLLSTI
MQLSIAVSIAAASLLLDSFGGHSATSGKAVETAFHATFATIGAIAAASSFIFALVDKDKGITRKRRKAA

Specific function: Unknown

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=467, Percent_Identity=46.8950749464668, Blast_Score=391, Evalue=1e-110,
Organism=Escherichia coli, GI1788392, Length=474, Percent_Identity=40.9282700421941, Blast_Score=325, Evalue=4e-90,
Organism=Escherichia coli, GI1788710, Length=322, Percent_Identity=31.9875776397516, Blast_Score=132, Evalue=4e-32,
Organism=Escherichia coli, GI1789042, Length=346, Percent_Identity=28.0346820809249, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI87081983, Length=406, Percent_Identity=27.5862068965517, Blast_Score=122, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6322958, Length=360, Percent_Identity=26.3888888888889, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321663, Length=153, Percent_Identity=30.718954248366, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6325455, Length=153, Percent_Identity=30.718954248366, Blast_Score=74, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6319770, Length=276, Percent_Identity=25.7246376811594, Blast_Score=65, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6323735, Length=159, Percent_Identity=30.188679245283, Blast_Score=63, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 50108; Mature: 50108

Theoretical pI: Translated: 10.76; Mature: 10.76

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLPTSSKALRYLPFLVASAFFMQMLDATILNTAIPTIARSFNSHPLQLHSLVTAYMLT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VCVLIPASGWISDKLGSRRTFLLAISVFSVGSLLCALSASVTMMTVCRVIQGIGGAFLMP
HHHHHCCCCCHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
VGRLVILRSYPRSMFVNVLNIVTIPALLGPLLGPVLGGIIVQYASWHWIFLINIPVGMAG
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHH
LWATWKLMPDLKAVREQKFDWPGFFLFSSSAVLATMGLSSAGGITDKPRMVILTSAGGIL
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHCCCCCCCCCCCCCEEEEECCCHHH
QLLYWVLAFRSKAPIFSPALFRIRNFAIGIAGNIVCRLGGSCLPYLMPLFFQVVLGYSAL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
QSGMSLIPLALSNLLAKTVAPRLLGKFGYRNIMVVNTFTIGSLLASFRFVGPGTHELILL
HCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHCCCCCHHHHHHH
GMLALLGGANSIQFTCMNTLTLIDLPNADASSGNSLLSTIMQLSIAVSIAAASLLLDSFG
HHHHHHCCCCCEEEEEECEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
GHSATSGKAVETAFHATFATIGAIAAASSFIFALVDKDKGITRKRRKAA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHCCC
>Mature Secondary Structure
MEKLPTSSKALRYLPFLVASAFFMQMLDATILNTAIPTIARSFNSHPLQLHSLVTAYMLT
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
VCVLIPASGWISDKLGSRRTFLLAISVFSVGSLLCALSASVTMMTVCRVIQGIGGAFLMP
HHHHHCCCCCHHHHCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
VGRLVILRSYPRSMFVNVLNIVTIPALLGPLLGPVLGGIIVQYASWHWIFLINIPVGMAG
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCHHH
LWATWKLMPDLKAVREQKFDWPGFFLFSSSAVLATMGLSSAGGITDKPRMVILTSAGGIL
HHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHCCCCCCCCCCCCCEEEEECCCHHH
QLLYWVLAFRSKAPIFSPALFRIRNFAIGIAGNIVCRLGGSCLPYLMPLFFQVVLGYSAL
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
QSGMSLIPLALSNLLAKTVAPRLLGKFGYRNIMVVNTFTIGSLLASFRFVGPGTHELILL
HCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHCCCCCHHHHHHH
GMLALLGGANSIQFTCMNTLTLIDLPNADASSGNSLLSTIMQLSIAVSIAAASLLLDSFG
HHHHHHCCCCCEEEEEECEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
GHSATSGKAVETAFHATFATIGAIAAASSFIFALVDKDKGITRKRRKAA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]