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The map label for this gene is truB [H]

Identifier: 187735254

GI number: 187735254

Start: 883280

End: 884014

Strand: Reverse

Name: truB [H]

Synonym: Amuc_0749

Alternate gene names: 187735254

Gene position: 884014-883280 (Counterclockwise)

Preceding gene: 187735255

Following gene: 187735253

Centisome position: 33.18

GC content: 55.24

Gene sequence:

>735_bases
ATGAATGTTTCAGATTCCATCCAGGTTCCTTCGGGCGTCCTGTTGATTGACAAGGCGCGGGACATGACTTCCCACGATGT
GGTGGCAATTGCGCGCCGTTCCCTGGGAATTAAGAAAATAGGCCATTGCGGCACGCTGGATCCCATGGCTACGGGATTGC
TGATGCTGGTGGTAGGGAAGGCGACCAAGCTTCAGGACAAGTTGATGTGCGAACACAAGGAATACGCCGGAACCCTTATG
CTGGGCGTGGAGACTTCCTCTCAAGATGCCATGGGAGAAATAGTGGCGGAGTATTCCGTAGACGGAGTGACGGAACAGGC
CGTGCGTGAAGCGTTTGACCGGTTTGACGGAGCTTTTGAACAAATTCCTCCGATGGTTTCCGCCATCAAAAAGGACGGGG
TGCCGCTGTACAAACTGGCCCGGAAAGGTCAGGAAATTGTGCGGGAGCCGCGGCCTGTGGAAGTGTTTGGCCACGGAATT
TCACGCGTGGCCATTCCGGAAGTGGATTTTACCGTTCAATGTTCCAAAGGTTTTTATGTGCGGTCCTATGCTTATGATAT
TGGGAAGGCATTGGGCTGCGGAGCGCATTTGAGCGCCCTCCGGCGCACCAGGTCTGGTTCTTTTACGCTGGACCGCGCCA
TTACGGTGGACACCCTGAAAACCGCGCCCAGGGAGGAACTGTTCCGGGCCATGCTTTCCCTGAAGGAGCTGGCGGATATT
CTGATTGCCGGGTAG

Upstream 100 bases:

>100_bases
CCGCCGGAGTGCGTATCCGGGGGCCTATTGAAGAAGTGCGCCTTACTATCCTGAAGGCCATGCGGGAAGAAGTGGAACGC
GTGATCCGACAGAAAATTTC

Downstream 100 bases:

>100_bases
CATATGGTCATTTACCGGGAATTCAAGGAATTGAAAAGCCTTGCCGCCCCCGTACACTGGGCCGTGGGGATGTTTGACGG
GGTGCATCTGGGACATGCCG

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MNVSDSIQVPSGVLLIDKARDMTSHDVVAIARRSLGIKKIGHCGTLDPMATGLLMLVVGKATKLQDKLMCEHKEYAGTLM
LGVETSSQDAMGEIVAEYSVDGVTEQAVREAFDRFDGAFEQIPPMVSAIKKDGVPLYKLARKGQEIVREPRPVEVFGHGI
SRVAIPEVDFTVQCSKGFYVRSYAYDIGKALGCGAHLSALRRTRSGSFTLDRAITVDTLKTAPREELFRAMLSLKELADI
LIAG

Sequences:

>Translated_244_residues
MNVSDSIQVPSGVLLIDKARDMTSHDVVAIARRSLGIKKIGHCGTLDPMATGLLMLVVGKATKLQDKLMCEHKEYAGTLM
LGVETSSQDAMGEIVAEYSVDGVTEQAVREAFDRFDGAFEQIPPMVSAIKKDGVPLYKLARKGQEIVREPRPVEVFGHGI
SRVAIPEVDFTVQCSKGFYVRSYAYDIGKALGCGAHLSALRRTRSGSFTLDRAITVDTLKTAPREELFRAMLSLKELADI
LIAG
>Mature_244_residues
MNVSDSIQVPSGVLLIDKARDMTSHDVVAIARRSLGIKKIGHCGTLDPMATGLLMLVVGKATKLQDKLMCEHKEYAGTLM
LGVETSSQDAMGEIVAEYSVDGVTEQAVREAFDRFDGAFEQIPPMVSAIKKDGVPLYKLARKGQEIVREPRPVEVFGHGI
SRVAIPEVDFTVQCSKGFYVRSYAYDIGKALGCGAHLSALRRTRSGSFTLDRAITVDTLKTAPREELFRAMLSLKELADI
LIAG

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=207, Percent_Identity=34.7826086956522, Blast_Score=122, Evalue=2e-28,
Organism=Homo sapiens, GI215599015, Length=196, Percent_Identity=30.6122448979592, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI4503337, Length=196, Percent_Identity=30.6122448979592, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI2367200, Length=210, Percent_Identity=40.4761904761905, Blast_Score=157, Evalue=5e-40,
Organism=Caenorhabditis elegans, GI17553978, Length=198, Percent_Identity=28.7878787878788, Blast_Score=88, Evalue=4e-18,
Organism=Saccharomyces cerevisiae, GI6324037, Length=127, Percent_Identity=37.7952755905512, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323204, Length=208, Percent_Identity=30.2884615384615, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI281364189, Length=199, Percent_Identity=30.6532663316583, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI281364187, Length=199, Percent_Identity=30.6532663316583, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI281364185, Length=199, Percent_Identity=30.6532663316583, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI281364183, Length=199, Percent_Identity=30.6532663316583, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI62471759, Length=199, Percent_Identity=30.6532663316583, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI17975520, Length=199, Percent_Identity=30.6532663316583, Blast_Score=88, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 26560; Mature: 26560

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVSDSIQVPSGVLLIDKARDMTSHDVVAIARRSLGIKKIGHCGTLDPMATGLLMLVVGK
CCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHCC
ATKLQDKLMCEHKEYAGTLMLGVETSSQDAMGEIVAEYSVDGVTEQAVREAFDRFDGAFE
CHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHH
QIPPMVSAIKKDGVPLYKLARKGQEIVREPRPVEVFGHGISRVAIPEVDFTVQCSKGFYV
HCCHHHHHHHHCCCHHHHHHHCCHHHHHCCCCEEEECCCCCEEEECCCCEEEEECCCEEE
RSYAYDIGKALGCGAHLSALRRTRSGSFTLDRAITVDTLKTAPREELFRAMLSLKELADI
HHHHHHHHHHHCCHHHHHHHHHHCCCCEEECCEEEHHHHHCCCHHHHHHHHHHHHHHHHH
LIAG
HCCC
>Mature Secondary Structure
MNVSDSIQVPSGVLLIDKARDMTSHDVVAIARRSLGIKKIGHCGTLDPMATGLLMLVVGK
CCCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCHHHCCCCCCCCHHHHHHHHHHHCC
ATKLQDKLMCEHKEYAGTLMLGVETSSQDAMGEIVAEYSVDGVTEQAVREAFDRFDGAFE
CHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHH
QIPPMVSAIKKDGVPLYKLARKGQEIVREPRPVEVFGHGISRVAIPEVDFTVQCSKGFYV
HCCHHHHHHHHCCCHHHHHHHCCHHHHHCCCCEEEECCCCCEEEECCCCEEEEECCCEEE
RSYAYDIGKALGCGAHLSALRRTRSGSFTLDRAITVDTLKTAPREELFRAMLSLKELADI
HHHHHHHHHHHCCHHHHHHHHHHCCCCEEECCEEEHHHHHCCCHHHHHHHHHHHHHHHHH
LIAG
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA