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The map label for this gene is mnmE

Identifier: 187735151

GI number: 187735151

Start: 758906

End: 760255

Strand: Direct

Name: mnmE

Synonym: Amuc_0645

Alternate gene names: 187735151

Gene position: 758906-760255 (Clockwise)

Preceding gene: 187735150

Following gene: 187735152

Centisome position: 28.49

GC content: 62.07

Gene sequence:

>1350_bases
ATGACTGACCCGGAACGCACCATAGCCGCACAGGCCACCGCCGCAGGACAGGGGGCCATCGCCGTCATCCGCATGAGCGG
TCCCGGCTGCATGGACATCCTGAAACAATGCACCCCTGCGGCTTTCACGGAACGCCTCCGGCCGCGCCGCGCGACGCTGG
TCTGCATCCGGGACGCGGAAGGTGCCGCCATTGACCAGGCGCTTGTCACCTGGTTCCCCGCCCCGGCCAGTTATACGGGG
GAAGATACCGCGGAAATCTCCTGCCATGGCGGCATGCTGGTGACGGACCGCCTGCTGAAACGCCTCTACCAATGCGGGGC
CTCCCCAGCGGAACCGGGAGAATTTACAAAAAGGGCCTTTCTGAACGGGCGCATGGACCTGACCCAGGCGGAAGCCGTGA
TGGATGTGATTTCCGCCGGGAGCGACCTGGCCCTGAAAGCCGCGCAAAGCCAGCTGGACGGAGGCATCGGCGCCCAGGTG
GACGAACTGAAAGACAACCTGGTTCATGTGCTGGCCCACATTGAGGCCTATATTGATTTTCCGGATGAAGACATTTCCCC
GGACACCGCCTCCGATCTGCTGGCGCGCCTCCGGAACATGGAGGAAAAACTGGACACTCTCCTCAAAACTGCCGAAGGCG
GACGCCTGCTGCGGGAAGGCATCCGCACGGCGATCGCCGGACCTCCCAACGTGGGAAAATCCAGCCTGCTGAATACCCTG
CTGGGATATGACCGGGCCATTGTCAGCAATATTGCCGGTACCACGCGGGACACGGTGGAAGAATCCATTCAGCTGGCCGG
GCTGGCCCTGCGGCTGATTGATACGGCCGGTGTCCGCGAATCTTCCGACGTCATTGAACAGGCAGGCATTACCCGCACCA
ACAGGGCTCTTGAAACCGCGGATCTGGTTCTGGAAGTGGCGGATGCCTCCACGCCCCGGGTCAAAGACTTCCCCGCCCCT
GTTCTCACCGCGCCCCGGCTGCTTATTCTGAACAAGTGCGACCTGGGAATCCATCCGGACTGGAAGGCCGTGCCCGGCAT
CCGGTTTTCCTGCGCCACCGGGGAAGGAAGAAAGGAATTGGAGGAAGCCATTATCCAGGCCTTTTCCTCCTCCCTGCCCG
GTGAAACGGGCAGCTCCCTGGTAGCCATCAATGCCCGGCACCAGCATGAACTGGGCCTCTGCCGGGAGCATGTCCGGCTG
GCTTCGGAATCCATCTCCCGCCAGGAAAGTCCGGAATTCACAGCCCTGGAACTGAGAGAGGCCCTGACGCACCTGGGAGA
AATCACCGGAGCCGTGGATACGGAGGACGTGCTGGGCGCCATTTTTTCCTCCTTCTGCCTGGGGAAATAA

Upstream 100 bases:

>100_bases
GCTTTGACACGCCCCTCCGGGCCCTGGCCGTGGGACAGGTCTGCGCCTTTTACGACGGAGGCAAGTTGCTCGGGGGCGGC
TTTTTTGAATCCATAGAACC

Downstream 100 bases:

>100_bases
GAAGAAGCTTCCATCAACAGGTTTTTCTGATTCCGGAATCTTCTTTCCTCTCCATTCTGTGGAAGGATGGCAATCATCCA
AAAATTTATTTGCGGATATT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MTDPERTIAAQATAAGQGAIAVIRMSGPGCMDILKQCTPAAFTERLRPRRATLVCIRDAEGAAIDQALVTWFPAPASYTG
EDTAEISCHGGMLVTDRLLKRLYQCGASPAEPGEFTKRAFLNGRMDLTQAEAVMDVISAGSDLALKAAQSQLDGGIGAQV
DELKDNLVHVLAHIEAYIDFPDEDISPDTASDLLARLRNMEEKLDTLLKTAEGGRLLREGIRTAIAGPPNVGKSSLLNTL
LGYDRAIVSNIAGTTRDTVEESIQLAGLALRLIDTAGVRESSDVIEQAGITRTNRALETADLVLEVADASTPRVKDFPAP
VLTAPRLLILNKCDLGIHPDWKAVPGIRFSCATGEGRKELEEAIIQAFSSSLPGETGSSLVAINARHQHELGLCREHVRL
ASESISRQESPEFTALELREALTHLGEITGAVDTEDVLGAIFSSFCLGK

Sequences:

>Translated_449_residues
MTDPERTIAAQATAAGQGAIAVIRMSGPGCMDILKQCTPAAFTERLRPRRATLVCIRDAEGAAIDQALVTWFPAPASYTG
EDTAEISCHGGMLVTDRLLKRLYQCGASPAEPGEFTKRAFLNGRMDLTQAEAVMDVISAGSDLALKAAQSQLDGGIGAQV
DELKDNLVHVLAHIEAYIDFPDEDISPDTASDLLARLRNMEEKLDTLLKTAEGGRLLREGIRTAIAGPPNVGKSSLLNTL
LGYDRAIVSNIAGTTRDTVEESIQLAGLALRLIDTAGVRESSDVIEQAGITRTNRALETADLVLEVADASTPRVKDFPAP
VLTAPRLLILNKCDLGIHPDWKAVPGIRFSCATGEGRKELEEAIIQAFSSSLPGETGSSLVAINARHQHELGLCREHVRL
ASESISRQESPEFTALELREALTHLGEITGAVDTEDVLGAIFSSFCLGK
>Mature_448_residues
TDPERTIAAQATAAGQGAIAVIRMSGPGCMDILKQCTPAAFTERLRPRRATLVCIRDAEGAAIDQALVTWFPAPASYTGE
DTAEISCHGGMLVTDRLLKRLYQCGASPAEPGEFTKRAFLNGRMDLTQAEAVMDVISAGSDLALKAAQSQLDGGIGAQVD
ELKDNLVHVLAHIEAYIDFPDEDISPDTASDLLARLRNMEEKLDTLLKTAEGGRLLREGIRTAIAGPPNVGKSSLLNTLL
GYDRAIVSNIAGTTRDTVEESIQLAGLALRLIDTAGVRESSDVIEQAGITRTNRALETADLVLEVADASTPRVKDFPAPV
LTAPRLLILNKCDLGIHPDWKAVPGIRFSCATGEGRKELEEAIIQAFSSSLPGETGSSLVAINARHQHELGLCREHVRLA
SESISRQESPEFTALELREALTHLGEITGAVDTEDVLGAIFSSFCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=464, Percent_Identity=37.9310344827586, Blast_Score=243, Evalue=3e-64,
Organism=Homo sapiens, GI193082988, Length=496, Percent_Identity=36.2903225806452, Blast_Score=234, Evalue=2e-61,
Organism=Homo sapiens, GI193082993, Length=450, Percent_Identity=36.6666666666667, Blast_Score=229, Evalue=5e-60,
Organism=Escherichia coli, GI2367268, Length=463, Percent_Identity=39.9568034557235, Blast_Score=297, Evalue=7e-82,
Organism=Escherichia coli, GI87082120, Length=92, Percent_Identity=43.4782608695652, Blast_Score=75, Evalue=6e-15,
Organism=Caenorhabditis elegans, GI17507259, Length=458, Percent_Identity=29.6943231441048, Blast_Score=178, Evalue=6e-45,
Organism=Saccharomyces cerevisiae, GI6323665, Length=497, Percent_Identity=29.7786720321932, Blast_Score=188, Evalue=1e-48,
Organism=Drosophila melanogaster, GI45550813, Length=480, Percent_Identity=33.5416666666667, Blast_Score=216, Evalue=2e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_AKKM8 (B2UPK5)

Other databases:

- EMBL:   CP001071
- RefSeq:   YP_001877263.1
- GeneID:   6274151
- GenomeReviews:   CP001071_GR
- KEGG:   amu:Amuc_0645
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 47959; Mature: 47828

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: BINDING 24-24 BINDING 85-85 BINDING 124-124 BINDING 449-449

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDPERTIAAQATAAGQGAIAVIRMSGPGCMDILKQCTPAAFTERLRPRRATLVCIRDAE
CCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHCCCHHHHHHCCCCCEEEEEEECCC
GAAIDQALVTWFPAPASYTGEDTAEISCHGGMLVTDRLLKRLYQCGASPAEPGEFTKRAF
CCHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
LNGRMDLTQAEAVMDVISAGSDLALKAAQSQLDGGIGAQVDELKDNLVHVLAHIEAYIDF
HCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC
PDEDISPDTASDLLARLRNMEEKLDTLLKTAEGGRLLREGIRTAIAGPPNVGKSSLLNTL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHH
LGYDRAIVSNIAGTTRDTVEESIQLAGLALRLIDTAGVRESSDVIEQAGITRTNRALETA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHH
DLVLEVADASTPRVKDFPAPVLTAPRLLILNKCDLGIHPDWKAVPGIRFSCATGEGRKEL
HHHHHHCCCCCCCCCCCCCCHHHCCCEEEEECCCCCCCCCCCCCCCCEEEECCCCCHHHH
EEAIIQAFSSSLPGETGSSLVAINARHQHELGLCREHVRLASESISRQESPEFTALELRE
HHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
ALTHLGEITGAVDTEDVLGAIFSSFCLGK
HHHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TDPERTIAAQATAAGQGAIAVIRMSGPGCMDILKQCTPAAFTERLRPRRATLVCIRDAE
CCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHCCCHHHHHHCCCCCEEEEEEECCC
GAAIDQALVTWFPAPASYTGEDTAEISCHGGMLVTDRLLKRLYQCGASPAEPGEFTKRAF
CCHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHH
LNGRMDLTQAEAVMDVISAGSDLALKAAQSQLDGGIGAQVDELKDNLVHVLAHIEAYIDF
HCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCC
PDEDISPDTASDLLARLRNMEEKLDTLLKTAEGGRLLREGIRTAIAGPPNVGKSSLLNTL
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCHHHHHHHH
LGYDRAIVSNIAGTTRDTVEESIQLAGLALRLIDTAGVRESSDVIEQAGITRTNRALETA
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHH
DLVLEVADASTPRVKDFPAPVLTAPRLLILNKCDLGIHPDWKAVPGIRFSCATGEGRKEL
HHHHHHCCCCCCCCCCCCCCHHHCCCEEEEECCCCCCCCCCCCCCCCEEEECCCCCHHHH
EEAIIQAFSSSLPGETGSSLVAINARHQHELGLCREHVRLASESISRQESPEFTALELRE
HHHHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHH
ALTHLGEITGAVDTEDVLGAIFSSFCLGK
HHHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA