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The map label for this gene is miaA

Identifier: 187735148

GI number: 187735148

Start: 754979

End: 755863

Strand: Direct

Name: miaA

Synonym: Amuc_0642

Alternate gene names: 187735148

Gene position: 754979-755863 (Clockwise)

Preceding gene: 187735147

Following gene: 187735150

Centisome position: 28.34

GC content: 62.15

Gene sequence:

>885_bases
ATGACCCTGTTTCTGGCAGGTCCCACCGGATCGGGCAAATCCGCGGTAGCCGTGGAACTGGCGGAGATGCTGGACGCAGA
AATCGTCAGTTCCGACGCTTACCAGGTGTACCGGGAGCTGCCCATCCTGACGGCGGCGCCTTCCCCGGCAGACCGGGAAC
GGGTCCCCCACCACATGGTTTCCATCATTCCGGTCCAAAAGAACTGGAATGCCACGGAACACTACCACCGCGCCATGCGC
TGCATGGAAGAAATTCACGCACGCGGAAAAATCGCCATCGTCACGGGCGGTTCCGGCCTGTACTTCAAATTCCTCTCCCA
CGGCCTGTCGGAAGCGCCGCCGGGGGACGCTGCTCTCCGCGCCGCTTTCGCGAACTGTTCCACGGAAGCGCTATACGCCA
GACTCTCTTCCCTGGATCCGGAAGGAGCGGCCTCGACGGATGCCTCCAACCGGCGTTATGTGGAACGCAATCTGGAAATC
GTCCTTGCCGGAGGAAAACCTCTTTCCTTCTGGAAGAGGAACTGGTTGACCCCGCCGCGCGGGCCCGGCTGGGTCATCAG
CAGGGATGTTCCTGAACTGGACGAAAGAATAGCCCACCGTGCCGCACGCATGATGCAGGAGGGGGCCGTGGAGGAAACGG
CTTCCCTCGGCCCATGCTCCGCCACAGCGGAACGCACCCTGGGCCTGGCGTTGATACGAAGCATGCTGCGCGGGGAAATC
TCCCGTGAAAACTGCCAGGGCCAGCTGGCCCTCGCCACCCGGCAATACGCCAAACGGCAGCGCACCTGGCTGAAACGCGA
ACAATGGCTCCGGAAACTTCCGGCTTCCCCTTCCGACTCTCCCCGGGCTCTTGCAGAACGCATCATGGAAGAACTGGGGC
ATTAA

Upstream 100 bases:

>100_bases
GCAGGAGCAGCTTTTAGGGCTTCACAGGAACAACAAACTTTATCCGCCATTACTCCACGTGATGATTGAATCTTCTCCAC
CCCATCAGGCAACACTTCCC

Downstream 100 bases:

>100_bases
TCCCTCTTCACACGGCGGTAAATATCCAAGTTCGTCACTTCTCTGAAAAAGGATCGTTCCCGGCAATATTCCGTACTTAG
CAAATCCCGCATAGCCCTCA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MTLFLAGPTGSGKSAVAVELAEMLDAEIVSSDAYQVYRELPILTAAPSPADRERVPHHMVSIIPVQKNWNATEHYHRAMR
CMEEIHARGKIAIVTGGSGLYFKFLSHGLSEAPPGDAALRAAFANCSTEALYARLSSLDPEGAASTDASNRRYVERNLEI
VLAGGKPLSFWKRNWLTPPRGPGWVISRDVPELDERIAHRAARMMQEGAVEETASLGPCSATAERTLGLALIRSMLRGEI
SRENCQGQLALATRQYAKRQRTWLKREQWLRKLPASPSDSPRALAERIMEELGH

Sequences:

>Translated_294_residues
MTLFLAGPTGSGKSAVAVELAEMLDAEIVSSDAYQVYRELPILTAAPSPADRERVPHHMVSIIPVQKNWNATEHYHRAMR
CMEEIHARGKIAIVTGGSGLYFKFLSHGLSEAPPGDAALRAAFANCSTEALYARLSSLDPEGAASTDASNRRYVERNLEI
VLAGGKPLSFWKRNWLTPPRGPGWVISRDVPELDERIAHRAARMMQEGAVEETASLGPCSATAERTLGLALIRSMLRGEI
SRENCQGQLALATRQYAKRQRTWLKREQWLRKLPASPSDSPRALAERIMEELGH
>Mature_293_residues
TLFLAGPTGSGKSAVAVELAEMLDAEIVSSDAYQVYRELPILTAAPSPADRERVPHHMVSIIPVQKNWNATEHYHRAMRC
MEEIHARGKIAIVTGGSGLYFKFLSHGLSEAPPGDAALRAAFANCSTEALYARLSSLDPEGAASTDASNRRYVERNLEIV
LAGGKPLSFWKRNWLTPPRGPGWVISRDVPELDERIAHRAARMMQEGAVEETASLGPCSATAERTLGLALIRSMLRGEIS
RENCQGQLALATRQYAKRQRTWLKREQWLRKLPASPSDSPRALAERIMEELGH

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=317, Percent_Identity=27.4447949526814, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1790613, Length=277, Percent_Identity=32.4909747292419, Blast_Score=120, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI25144712, Length=338, Percent_Identity=24.2603550295858, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6324848, Length=291, Percent_Identity=28.5223367697594, Blast_Score=127, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24649830, Length=328, Percent_Identity=23.1707317073171, Blast_Score=79, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_AKKM8 (B2UPK2)

Other databases:

- EMBL:   CP001071
- RefSeq:   YP_001877260.1
- GeneID:   6274157
- GenomeReviews:   CP001071_GR
- KEGG:   amu:Amuc_0642
- HOGENOM:   HBG559459
- OMA:   QNSESPY
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 32451; Mature: 32320

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLFLAGPTGSGKSAVAVELAEMLDAEIVSSDAYQVYRELPILTAAPSPADRERVPHHMV
CEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHCCHHHH
SIIPVQKNWNATEHYHRAMRCMEEIHARGKIAIVTGGSGLYFKFLSHGLSEAPPGDAALR
EEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCCCCCHHHHH
AAFANCSTEALYARLSSLDPEGAASTDASNRRYVERNLEIVLAGGKPLSFWKRNWLTPPR
HHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCEEEEECCCCHHHHHHCCCCCCC
GPGWVISRDVPELDERIAHRAARMMQEGAVEETASLGPCSATAERTLGLALIRSMLRGEI
CCCEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
SRENCQGQLALATRQYAKRQRTWLKREQWLRKLPASPSDSPRALAERIMEELGH
CHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
TLFLAGPTGSGKSAVAVELAEMLDAEIVSSDAYQVYRELPILTAAPSPADRERVPHHMV
EEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCCHHHCCHHHH
SIIPVQKNWNATEHYHRAMRCMEEIHARGKIAIVTGGSGLYFKFLSHGLSEAPPGDAALR
EEEEECCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHCCCCCCCCCHHHHH
AAFANCSTEALYARLSSLDPEGAASTDASNRRYVERNLEIVLAGGKPLSFWKRNWLTPPR
HHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCEEEEECCCCHHHHHHCCCCCCC
GPGWVISRDVPELDERIAHRAARMMQEGAVEETASLGPCSATAERTLGLALIRSMLRGEI
CCCEEEECCCHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
SRENCQGQLALATRQYAKRQRTWLKREQWLRKLPASPSDSPRALAERIMEELGH
CHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA