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The map label for this gene is wcaE

Identifier: 187733816

GI number: 187733816

Start: 1080524

End: 1081270

Strand: Direct

Name: wcaE

Synonym: SbBS512_E1177

Alternate gene names: 187733816

Gene position: 1080524-1081270 (Clockwise)

Preceding gene: 187734147

Following gene: 187732390

Centisome position: 23.41

GC content: 45.52

Gene sequence:

>747_bases
ATGTTGCTTAGCATAATCACTGTCGCGTTTCGTAACCTCGAAGGGATAGTCAAAACACATGCCTCGCTGGCGCATCTGGC
GCAGGTGGAAGATATCAGCTTCGAATGGATTGTTGTCGATGGCGGTTCCAACGACGGCACCCGTGAGTATCTGGAAAATC
TCAATGGTATCTTTAACCTACGCTTTGTCAGCGAGCCAGATAACGGTATCTACGACGCCATGAACAAAGGCATTGCGATG
GCACAAGGCAAGTTCGCGTTGTTTCTCAATTCGGGCGATATTTTTCATCAGAATGCCGCAAATTTTGTCCGTAAGTTAAA
AATGCAAAAAGATAACGTGATGATCACCGGCGATGCGCTGCTGGATTTTGGCGACGGGCATAAAATTAAACGTAGCGCCA
AACCGGGCTGGTATATTTATCACAGCCTGCCCGCCAGTCATCAGGCGATATTTTTCCCTGTATCCGGCTTGAAAAAATGG
CGTTATGACCTGGAATATAAAGTTTCTTCCGACTACGCGCTGGCAGCCAAAATGTATAAAGCCGGTTATGCATTTAAAAA
ACTCAATGGCCTGGTGTCTGAATTTTCCATGGGTGGGGTATCTACCACCAATAATATGGAATTGTGTGCTGACGCGAAAA
AAGTCCAACGACAAATATTACATGTGCCTGGCTTTTGGGCTGAATTATCCTGGCATTTACGCCAACGTACTACCTCAAAG
ACGAAAGCCTTATATAACAAAGTCTGA

Upstream 100 bases:

>100_bases
TTTTTTACCGGTTCGATATTTAGCCCGGAATATGCATTTTTAATCGTCTGTCCGTTTATTTTGCGAAAAGCGTTAAATAT
TACGAGGTAAGAATAAGAAC

Downstream 100 bases:

>100_bases
ATATAAGGAAAACCAATGCAAGATTTAAGCGGTTTCTCGGTGCCGAAAGGGTTCCGGGGCGGCAACGCTATTAAAGTGCA
ATTATGGTGGGCAGTACAGG

Product: putative glycosyl transferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MLLSIITVAFRNLEGIVKTHASLAHLAQVEDISFEWIVVDGGSNDGTREYLENLNGIFNLRFVSEPDNGIYDAMNKGIAM
AQGKFALFLNSGDIFHQNAANFVRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQILHVPGFWAELSWHLRQRTTSK
TKALYNKV

Sequences:

>Translated_248_residues
MLLSIITVAFRNLEGIVKTHASLAHLAQVEDISFEWIVVDGGSNDGTREYLENLNGIFNLRFVSEPDNGIYDAMNKGIAM
AQGKFALFLNSGDIFHQNAANFVRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQILHVPGFWAELSWHLRQRTTSK
TKALYNKV
>Mature_248_residues
MLLSIITVAFRNLEGIVKTHASLAHLAQVEDISFEWIVVDGGSNDGTREYLENLNGIFNLRFVSEPDNGIYDAMNKGIAM
AQGKFALFLNSGDIFHQNAANFVRKLKMQKDNVMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQILHVPGFWAELSWHLRQRTTSK
TKALYNKV

Specific function: Slime polysaccharide colanic acid biosynthesis. [C]

COG id: COG0463

COG function: function code M; Glycosyltransferases involved in cell wall biogenesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788368, Length=248, Percent_Identity=100, Blast_Score=515, Evalue=1e-147,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): WCAE_ECOLI (P71239)

Other databases:

- EMBL:   U38473
- EMBL:   U00096
- EMBL:   AP009048
- PIR:   F64971
- RefSeq:   AP_002655.1
- RefSeq:   NP_416559.1
- ProteinModelPortal:   P71239
- DIP:   DIP-11121N
- STRING:   P71239
- EnsemblBacteria:   EBESCT00000000015
- EnsemblBacteria:   EBESCT00000015203
- GeneID:   946543
- GenomeReviews:   AP009048_GR
- GenomeReviews:   U00096_GR
- KEGG:   ecj:JW2040
- KEGG:   eco:b2055
- EchoBASE:   EB3343
- EcoGene:   EG13573
- eggNOG:   COG0463
- GeneTree:   EBGT00050000008876
- HOGENOM:   HBG417266
- OMA:   FHQDAAN
- ProtClustDB:   PRK10063
- BioCyc:   EcoCyc:G7100-MONOMER
- Genevestigator:   P71239
- InterPro:   IPR001173

Pfam domain/function: PF00535 Glycos_transf_2

EC number: NA

Molecular weight: Translated: 27894; Mature: 27894

Theoretical pI: Translated: 9.78; Mature: 9.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLSIITVAFRNLEGIVKTHASLAHLAQVEDISFEWIVVDGGSNDGTREYLENLNGIFNL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHCCCEEEE
RFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQNAANFVRKLKMQKDNVMITGDAL
EEECCCCCCHHHHHCCCEEEECCCEEEEEECCCEEHHHHHHHHHHHHCCCCCEEEEECEE
LDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKWRYDLEYKVSSDYALAAKMYK
EECCCCCEEECCCCCCEEEEECCCCCCCEEEEECCCHHHHCCCEEEEECCCHHHHHHHHH
AGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQILHVPGFWAELSWHLRQRTTSK
HCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
TKALYNKV
HHHHHHCC
>Mature Secondary Structure
MLLSIITVAFRNLEGIVKTHASLAHLAQVEDISFEWIVVDGGSNDGTREYLENLNGIFNL
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHCCCEEEE
RFVSEPDNGIYDAMNKGIAMAQGKFALFLNSGDIFHQNAANFVRKLKMQKDNVMITGDAL
EEECCCCCCHHHHHCCCEEEECCCEEEEEECCCEEHHHHHHHHHHHHCCCCCEEEEECEE
LDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKWRYDLEYKVSSDYALAAKMYK
EECCCCCEEECCCCCCEEEEECCCCCCCEEEEECCCHHHHCCCEEEEECCCHHHHHHHHH
AGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQILHVPGFWAELSWHLRQRTTSK
HCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
TKALYNKV
HHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8759852; 9278503