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The map label for this gene is rfbX [H]

Identifier: 187733398

GI number: 187733398

Start: 1103246

End: 1104463

Strand: Direct

Name: rfbX [H]

Synonym: SbBS512_E1197

Alternate gene names: 187733398

Gene position: 1103246-1104463 (Clockwise)

Preceding gene: 187731823

Following gene: 187732137

Centisome position: 23.9

GC content: 28.74

Gene sequence:

>1218_bases
ATGAAAAAAAATATAATATATCTTTTTTTTGTGCAGGGCACAAACTATCTGTTTCCACTAATAACATTGCCATATTTGAT
TCGCATGTTAGGGAGTAACTCTTTTGGCATATATGCAATGATACTTGCTGGAATACAGTATGTTAATATAATTGTTGATT
TCGGTTTTAACTTCACTGCTACAAAACTAATCTCAATATATAAAAATAATGAAAATGAGAAAAATAAAATATTCACTGCA
ACAATTATAATTAAATTTATTTTATTCTGTCTTTGCCTTTCTGTTCTATTATTATTATCTTTGGTATTAGAACAGGGAAA
ATATATACAATATATAATAATAGGTTCTATCGCTGTAATTGGGAATATGCTCTTACCTGTTTGGTTTTTTCAGGGAATAG
AGAGAATGAGTAAGATTGCATTATTTTCTACTTTATCTAAACTTTCATCATTACTATTTATTTTTTTTACGGTAAAATCA
TCAGATGATGTTGGACTAGCTATAGCAAGTTACTCATTAGGCTTGCTATTTACTGGGCTATTATCAGTTTATGTTATCTT
TCATTATAAATATGTTAAATTTACAAAGGTGAGTTTAAAGTTTATAATCAAATTAGCTAAAGATAGTGTTGATATGTTTA
TATCAAACATTACTGTTAGTTTTTATACTACACTTAATACAATTTTAGTTGGTGCTATTGCTGGCCCTGCAACAGCAGGT
TACTTTTCGGCTGCGGATAAATTAAGGATAGCGGTTCAAGGACTTTTAACACCTTTGCAGCAAGCAATTTTTCCAAGAGT
TACAAGTTTGATTAATAATGACTATAAGCTGAATGATGTATTAAAAACATTTGGCTATAAAATGATTGGCTTTGGATTTT
TTCTATCACTAAGCATTGCGATCATTGGAGTTCCATTATCCGAGTGGTATTTTGGTAACAATAATGCAATATCATCAAAA
ATACTTTTAATACTTAGTCCACTTCCTTTTGTCGTGTCTATTGGTATTGTATTCGGGCAATGGTGGCTAATTACCACTAA
TAGGACAAGAATAATCAGAAAAGTATATTTTAATTTAAGCATGCTACATGTTTTTATTGCTTATTTCTTAATTAAGCACA
TTCCGAATTATGGCGTAGCAATATCAATAGTTTTTACTGAAACTATCATATCATTATTACTTACAATTTTTGCTATAAAA
AACTCAGTTAATCATTGA

Upstream 100 bases:

>100_bases
CCCAGAAAATTTACAGAGTAAAAATGTTAATCTTGGGTAAAAGAGATGTATATTAACCATATCTCTTTTTCAAATATTTT
TTAGAGTAAAAAATCTTTTC

Downstream 100 bases:

>100_bases
AAAAGAGGAAACGAATTGAAAGTTATAGCATTTTATTTACCACAATATCATGTGATTCCTGAAAATGACAAATGGTGGGG
AGAAGGTTTTACAGAATGGA

Product: Wzx

Products: lipopolysaccharide [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MKKNIIYLFFVQGTNYLFPLITLPYLIRMLGSNSFGIYAMILAGIQYVNIIVDFGFNFTATKLISIYKNNENEKNKIFTA
TIIIKFILFCLCLSVLLLLSLVLEQGKYIQYIIIGSIAVIGNMLLPVWFFQGIERMSKIALFSTLSKLSSLLFIFFTVKS
SDDVGLAIASYSLGLLFTGLLSVYVIFHYKYVKFTKVSLKFIIKLAKDSVDMFISNITVSFYTTLNTILVGAIAGPATAG
YFSAADKLRIAVQGLLTPLQQAIFPRVTSLINNDYKLNDVLKTFGYKMIGFGFFLSLSIAIIGVPLSEWYFGNNNAISSK
ILLILSPLPFVVSIGIVFGQWWLITTNRTRIIRKVYFNLSMLHVFIAYFLIKHIPNYGVAISIVFTETIISLLLTIFAIK
NSVNH

Sequences:

>Translated_405_residues
MKKNIIYLFFVQGTNYLFPLITLPYLIRMLGSNSFGIYAMILAGIQYVNIIVDFGFNFTATKLISIYKNNENEKNKIFTA
TIIIKFILFCLCLSVLLLLSLVLEQGKYIQYIIIGSIAVIGNMLLPVWFFQGIERMSKIALFSTLSKLSSLLFIFFTVKS
SDDVGLAIASYSLGLLFTGLLSVYVIFHYKYVKFTKVSLKFIIKLAKDSVDMFISNITVSFYTTLNTILVGAIAGPATAG
YFSAADKLRIAVQGLLTPLQQAIFPRVTSLINNDYKLNDVLKTFGYKMIGFGFFLSLSIAIIGVPLSEWYFGNNNAISSK
ILLILSPLPFVVSIGIVFGQWWLITTNRTRIIRKVYFNLSMLHVFIAYFLIKHIPNYGVAISIVFTETIISLLLTIFAIK
NSVNH
>Mature_405_residues
MKKNIIYLFFVQGTNYLFPLITLPYLIRMLGSNSFGIYAMILAGIQYVNIIVDFGFNFTATKLISIYKNNENEKNKIFTA
TIIIKFILFCLCLSVLLLLSLVLEQGKYIQYIIIGSIAVIGNMLLPVWFFQGIERMSKIALFSTLSKLSSLLFIFFTVKS
SDDVGLAIASYSLGLLFTGLLSVYVIFHYKYVKFTKVSLKFIIKLAKDSVDMFISNITVSFYTTLNTILVGAIAGPATAG
YFSAADKLRIAVQGLLTPLQQAIFPRVTSLINNDYKLNDVLKTFGYKMIGFGFFLSLSIAIIGVPLSEWYFGNNNAISSK
ILLILSPLPFVVSIGIVFGQWWLITTNRTRIIRKVYFNLSMLHVFIAYFLIKHIPNYGVAISIVFTETIISLLLTIFAIK
NSVNH

Specific function: May be involved in the translocation process of the nascent O-polysaccharide molecules and/or its ligation to lipid A core units [H]

COG id: COG2244

COG function: function code R; Membrane protein involved in the export of O-antigen and teichoic acid

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788349, Length=410, Percent_Identity=32.9268292682927, Blast_Score=197, Evalue=7e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001991
- InterPro:   IPR002797 [H]

Pfam domain/function: PF01943 Polysacc_synt [H]

EC number: NA

Molecular weight: Translated: 45554; Mature: 45554

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKNIIYLFFVQGTNYLFPLITLPYLIRMLGSNSFGIYAMILAGIQYVNIIVDFGFNFTA
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEECCCCHHH
TKLISIYKNNENEKNKIFTATIIIKFILFCLCLSVLLLLSLVLEQGKYIQYIIIGSIAVI
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHH
GNMLLPVWFFQGIERMSKIALFSTLSKLSSLLFIFFTVKSSDDVGLAIASYSLGLLFTGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCEEEHHHHHHHHHHHHH
LSVYVIFHYKYVKFTKVSLKFIIKLAKDSVDMFISNITVSFYTTLNTILVGAIAGPATAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
YFSAADKLRIAVQGLLTPLQQAIFPRVTSLINNDYKLNDVLKTFGYKMIGFGFFLSLSIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHH
IIGVPLSEWYFGNNNAISSKILLILSPLPFVVSIGIVFGQWWLITTNRTRIIRKVYFNLS
HEEEEHHHCEECCCCCCCHHHEEEECCHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHH
MLHVFIAYFLIKHIPNYGVAISIVFTETIISLLLTIFAIKNSVNH
HHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKNIIYLFFVQGTNYLFPLITLPYLIRMLGSNSFGIYAMILAGIQYVNIIVDFGFNFTA
CCCCEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHEECCCCHHH
TKLISIYKNNENEKNKIFTATIIIKFILFCLCLSVLLLLSLVLEQGKYIQYIIIGSIAVI
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHH
GNMLLPVWFFQGIERMSKIALFSTLSKLSSLLFIFFTVKSSDDVGLAIASYSLGLLFTGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCEEEHHHHHHHHHHHHH
LSVYVIFHYKYVKFTKVSLKFIIKLAKDSVDMFISNITVSFYTTLNTILVGAIAGPATAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
YFSAADKLRIAVQGLLTPLQQAIFPRVTSLINNDYKLNDVLKTFGYKMIGFGFFLSLSIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHHHHHHHHH
IIGVPLSEWYFGNNNAISSKILLILSPLPFVVSIGIVFGQWWLITTNRTRIIRKVYFNLS
HEEEEHHHCEECCCCCCCHHHEEEECCHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHH
MLHVFIAYFLIKHIPNYGVAISIVFTETIISLLLTIFAIKNSVNH
HHHHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lipopolysaccharide [Cytoplasm] [C]

Specific reaction: lipopolysaccharide [Cytoplasm] = lipopolysaccharide [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7692219 [H]