Click here to switch to the map view.

The map label for this gene is gspF

Identifier: 187732930

GI number: 187732930

Start: 3170018

End: 3171241

Strand: Reverse

Name: gspF

Synonym: SbBS512_E3410

Alternate gene names: 187732930

Gene position: 3171241-3170018 (Counterclockwise)

Preceding gene: 187730235

Following gene: 187732466

Centisome position: 68.7

GC content: 58.09

Gene sequence:

>1224_bases
ATGGCACTGTTTTACTATCAGGCGCTGGAGCGTAATGGTCGCAAAACCAAAGGCATGATTGAGGCGGATTCCGCGCGTCA
TGCCCGCCAGTTGTTGCGCGGTAAAGAGCTTATCCCCGTGCACATTGAAGCCAGGATGAATGCATCGGCAGGGGGAATGT
TGCAGCGTCGGCGGCACGCACATCGTCGCGTGGCGGCGGCAGATCTGGCGCTGTTCACGCGCCAACTGGCAATGCTGGTG
CAGGCAGCAATGCCGTTGGAAACCTGCTTGCAGGCGGTCAGTGAGCAAAGTGAAAAACTGCATGTGAAGAGCCTCGGAAT
GGCGCTGCGCAGCCGGATTCAGGAAGGTTATACCCTGTCGGACAGCCTGCGCGAACATCCCCGCGTCTTTGATTCCCTGT
TTTGTTCGATGGTCGCTGCCGGAGAAAAATCCGGGCATCTCGACGTGGTGCTCAATCGCCTGGCAGATTACACCGAACAG
CGACAGCGCCTGAAATCACGTCTATTGCAGGCCATGCTCTATCCGCTGGTTATGCTGGTGGTGGCAACGGGCGTGGTCAC
TATTTTGCTGACGGCAGTGGTGCCGAAAATCATCGAACAGTTTGATCATCTCGGACACGCGCTACCCTCCTCCACCCGTG
CGCTTATCGCCATGAGTGATGCGTTACAGGCCAGCGGCGTTTACTGGCTGGCGGGATTGCTGGCGCTTCTGGTGCTGGGG
CAATGGCTACTTAAAAATCCGACTATGCGCCTGCGCTGGGATAAAACCTTGCTGCGTCTGCCCGTGACGGGCCGTGTTGC
GCGCGGGCTGAATACGGCGCGTTTTTCCCGCACATTAAGCATCCTCACCGCCAGCAGTGTTCCGCTACTGGAAGGCATTC
AGACCGCTGCCGCCGTGTCGGCAAATCGCTATGTCGAACAACAACTGCTGCTGGCGGCAGATCGCGTCCGCGAAGGAAGC
AGTCTGCGCGCCGCGCTGGTGGAGTTGCGCCTGTTCCCGCCGATGATGCTGTACATGATCGCTTCCGGCGAACAGAGCGG
CGAACTGGAAACCATGCTTGAGCAGGCCGCTGTTAACCAGGAACGGGAATTTGATACCCAGGTGGGGCTGGCGTTGGGGC
TGTTTGAGCCTGCGCTGGTGGTGATGATGGCGGGCGTGGTGCTGTTTATTGTCATCGCCATCCTCGAGCCGATGCTGCAA
CTGAATAATATGGTTGGAATGTAA

Upstream 100 bases:

>100_bases
TCCGGCAACACTCTGCGGGTATCCGCAGCAACGGCATTGAGAAAGTGCTCGCCGGAGAAACCTCTCTCGATGAAGTTCTG
CGGGTAACCATGGAGGCGTA

Downstream 100 bases:

>100_bases
TTTACGGAGTTATCACATGAATTCGTTATCCCGCACACACAAACCACGGGCAGGTTTTACCCTGCTGGAAGTGATGGTGG
TGATTGTTATTCTTGGCGTC

Product: general secretion pathway protein GspF

Products: NA

Alternate protein names: Cholera toxin secretion protein epsF [H]

Number of amino acids: Translated: 407; Mature: 406

Protein sequence:

>407_residues
MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEARMNASAGGMLQRRRHAHRRVAAADLALFTRQLAMLV
QAAMPLETCLQAVSEQSEKLHVKSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
RQRLKSRLLQAMLYPLVMLVVATGVVTILLTAVVPKIIEQFDHLGHALPSSTRALIAMSDALQASGVYWLAGLLALLVLG
QWLLKNPTMRLRWDKTLLRLPVTGRVARGLNTARFSRTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGS
SLRAALVELRLFPPMMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVIAILEPMLQ
LNNMVGM

Sequences:

>Translated_407_residues
MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEARMNASAGGMLQRRRHAHRRVAAADLALFTRQLAMLV
QAAMPLETCLQAVSEQSEKLHVKSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQ
RQRLKSRLLQAMLYPLVMLVVATGVVTILLTAVVPKIIEQFDHLGHALPSSTRALIAMSDALQASGVYWLAGLLALLVLG
QWLLKNPTMRLRWDKTLLRLPVTGRVARGLNTARFSRTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGS
SLRAALVELRLFPPMMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVIAILEPMLQ
LNNMVGM
>Mature_406_residues
ALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEARMNASAGGMLQRRRHAHRRVAAADLALFTRQLAMLVQ
AAMPLETCLQAVSEQSEKLHVKSLGMALRSRIQEGYTLSDSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQR
QRLKSRLLQAMLYPLVMLVVATGVVTILLTAVVPKIIEQFDHLGHALPSSTRALIAMSDALQASGVYWLAGLLALLVLGQ
WLLKNPTMRLRWDKTLLRLPVTGRVARGLNTARFSRTLSILTASSVPLLEGIQTAAAVSANRYVEQQLLLAADRVREGSS
LRAALVELRLFPPMMLYMIASGEQSGELETMLEQAAVNQEREFDTQVGLALGLFEPALVVMMAGVVLFIVIAILEPMLQL
NNMVGM

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG1459

COG function: function code NU; Type II secretory pathway, component PulF

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP F family [H]

Homologues:

Organism=Escherichia coli, GI1789724, Length=404, Percent_Identity=45.2970297029703, Blast_Score=331, Evalue=5e-92,
Organism=Escherichia coli, GI1786295, Length=409, Percent_Identity=25.1833740831296, Blast_Score=114, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003004
- InterPro:   IPR011850
- InterPro:   IPR018076
- InterPro:   IPR001992 [H]

Pfam domain/function: PF00482 GSPII_F [H]

EC number: NA

Molecular weight: Translated: 45015; Mature: 44884

Theoretical pI: Translated: 10.13; Mature: 10.13

Prosite motif: PS00874 T2SP_F

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEARMNASAGGMLQRRRHA
CCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHH
HRRVAAADLALFTRQLAMLVQAAMPLETCLQAVSEQSEKLHVKSLGMALRSRIQEGYTLS
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
DSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQRQRLKSRLLQAMLYPLVMLV
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VATGVVTILLTAVVPKIIEQFDHLGHALPSSTRALIAMSDALQASGVYWLAGLLALLVLG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
QWLLKNPTMRLRWDKTLLRLPVTGRVARGLNTARFSRTLSILTASSVPLLEGIQTAAAVS
HHHHCCCCEEEEECCHHHCCCCCHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ANRYVEQQLLLAADRVREGSSLRAALVELRLFPPMMLYMIASGEQSGELETMLEQAAVNQ
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHCC
EREFDTQVGLALGLFEPALVVMMAGVVLFIVIAILEPMLQLNNMVGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALFYYQALERNGRKTKGMIEADSARHARQLLRGKELIPVHIEARMNASAGGMLQRRRHA
CHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEEEEECCCCCCHHHHHHHHH
HRRVAAADLALFTRQLAMLVQAAMPLETCLQAVSEQSEKLHVKSLGMALRSRIQEGYTLS
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
DSLREHPRVFDSLFCSMVAAGEKSGHLDVVLNRLADYTEQRQRLKSRLLQAMLYPLVMLV
HHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VATGVVTILLTAVVPKIIEQFDHLGHALPSSTRALIAMSDALQASGVYWLAGLLALLVLG
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHH
QWLLKNPTMRLRWDKTLLRLPVTGRVARGLNTARFSRTLSILTASSVPLLEGIQTAAAVS
HHHHCCCCEEEEECCHHHCCCCCHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
ANRYVEQQLLLAADRVREGSSLRAALVELRLFPPMMLYMIASGEQSGELETMLEQAAVNQ
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCHHHHHHHHCCCCCCHHHHHHHHHHHCC
EREFDTQVGLALGLFEPALVVMMAGVVLFIVIAILEPMLQLNNMVGM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]