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The map label for this gene is yfcG [H]

Identifier: 186476743

GI number: 186476743

Start: 2239596

End: 2240297

Strand: Direct

Name: yfcG [H]

Synonym: Bphy_1988

Alternate gene names: 186476743

Gene position: 2239596-2240297 (Clockwise)

Preceding gene: 186476742

Following gene: 186476771

Centisome position: 64.37

GC content: 59.97

Gene sequence:

>702_bases
ATGATCGACGTCTACAGCTGGGCCACCCCGAACGGCCACAAGATCCACATCATGCTCGAGGAGACAGGCCTCGAATACAC
AGCACACCCAATCAACATCGGCGCCGGCGACCAGTTCGCGAGAGACTTCCTGGCAATCAGCCCCAACAATAAAATCCCCG
CCATCGTCGACACCGAAGGTCCGAAGCGCCCCGACGGCGCGCCGTTCTCCCTGTTCGAATCAGGCGCGATCCTGATCTAC
CTCGCAGAGAAGACAGGCAAATTCCTGCCCACCGATCCCGCCGCGCGTTACGAAACGCTGCAATGGCTGATGTTCCAGAT
GGGCGGCATCGGTCCGATGCTCGGCCAGACACACCACTTCCGCGTCTACGCGCCGCAGCCGATCGAATACGCGATCAACC
GCTATACGAACGAAGCAAAACGCCTCTACGGCGTGATAGATACGCAACTCGGCAAGACCCGCTATTTGGCCGGCAACGAC
TATACGATCGCCGATATTGCGACGTTTCCGTGGACCCGCTCATGGCAAAACCAGGGTTTGCAGATCGACGATTATCCGAA
CGTGAAACGCTGGCACGAAGAAATCGCCGCGCGCCCCGCCGTGCAACGCGGCGTCGAAGTGCTGGCGTCGGCGCGCGTGC
CGCTGATGGACGACAAGGCCAAGGAAGTGTTGTTCGGTGCGACACAGTACGCGAAGCACTGA

Upstream 100 bases:

>100_bases
TTGTTTGCGACGCTGGGTGTCAGTTTCTGGTCTCGCTGACGGATAGTCCCCAGAAACCGGCAACGCCACGCCGCAGGCAA
AAACTCAAAGGAGCACACCA

Downstream 100 bases:

>100_bases
ACATCGGCATCGCATGGTGCGAAGTCGCTTCGATGCACCTAGAAATAATGCCGCGTAATGAACTCTGCGACACACACGGG
ACGCTCGCTCCCCTTCCGCT

Product: glutathione S-transferase domain-containing protein

Products: HX; R-S-glutathione

Alternate protein names: GST-like protein yfcG [H]

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MIDVYSWATPNGHKIHIMLEETGLEYTAHPINIGAGDQFARDFLAISPNNKIPAIVDTEGPKRPDGAPFSLFESGAILIY
LAEKTGKFLPTDPAARYETLQWLMFQMGGIGPMLGQTHHFRVYAPQPIEYAINRYTNEAKRLYGVIDTQLGKTRYLAGND
YTIADIATFPWTRSWQNQGLQIDDYPNVKRWHEEIAARPAVQRGVEVLASARVPLMDDKAKEVLFGATQYAKH

Sequences:

>Translated_233_residues
MIDVYSWATPNGHKIHIMLEETGLEYTAHPINIGAGDQFARDFLAISPNNKIPAIVDTEGPKRPDGAPFSLFESGAILIY
LAEKTGKFLPTDPAARYETLQWLMFQMGGIGPMLGQTHHFRVYAPQPIEYAINRYTNEAKRLYGVIDTQLGKTRYLAGND
YTIADIATFPWTRSWQNQGLQIDDYPNVKRWHEEIAARPAVQRGVEVLASARVPLMDDKAKEVLFGATQYAKH
>Mature_233_residues
MIDVYSWATPNGHKIHIMLEETGLEYTAHPINIGAGDQFARDFLAISPNNKIPAIVDTEGPKRPDGAPFSLFESGAILIY
LAEKTGKFLPTDPAARYETLQWLMFQMGGIGPMLGQTHHFRVYAPQPIEYAINRYTNEAKRLYGVIDTQLGKTRYLAGND
YTIADIATFPWTRSWQNQGLQIDDYPNVKRWHEEIAARPAVQRGVEVLASARVPLMDDKAKEVLFGATQYAKH

Specific function: Has disulfide bond reductase activity (in vitro). Has low hydroperoxidase activity with cumene hydroperoxide. Has very low glutathione-S-transferase activity (in vitro) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1788640, Length=205, Percent_Identity=53.6585365853659, Blast_Score=219, Evalue=1e-58,
Organism=Escherichia coli, GI87082195, Length=218, Percent_Identity=49.0825688073394, Blast_Score=184, Evalue=5e-48,
Organism=Escherichia coli, GI87081789, Length=190, Percent_Identity=29.4736842105263, Blast_Score=80, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6324100, Length=237, Percent_Identity=31.2236286919831, Blast_Score=120, Evalue=2e-28,
Organism=Drosophila melanogaster, GI17864592, Length=201, Percent_Identity=30.8457711442786, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI28571670, Length=176, Percent_Identity=30.6818181818182, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI17933730, Length=231, Percent_Identity=27.7056277056277, Blast_Score=83, Evalue=1e-16,
Organism=Drosophila melanogaster, GI17864598, Length=174, Percent_Identity=31.6091954022989, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI85725204, Length=197, Percent_Identity=29.9492385786802, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI17737923, Length=197, Percent_Identity=29.9492385786802, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI17864594, Length=195, Percent_Identity=30.2564102564103, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24646249, Length=166, Percent_Identity=30.1204819277108, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI45549270, Length=195, Percent_Identity=26.1538461538462, Blast_Score=71, Evalue=5e-13,
Organism=Drosophila melanogaster, GI17864596, Length=196, Percent_Identity=23.469387755102, Blast_Score=68, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24646251, Length=200, Percent_Identity=27.5, Blast_Score=68, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: 2.5.1.18

Molecular weight: Translated: 26223; Mature: 26223

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDVYSWATPNGHKIHIMLEETGLEYTAHPINIGAGDQFARDFLAISPNNKIPAIVDTEG
CCCEEECCCCCCCEEEEEEECCCCEEEECEEECCCCHHHHHHEEEECCCCCCCEEEECCC
PKRPDGAPFSLFESGAILIYLAEKTGKFLPTDPAARYETLQWLMFQMGGIGPMLGQTHHF
CCCCCCCCEEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCEE
RVYAPQPIEYAINRYTNEAKRLYGVIDTQLGKTRYLAGNDYTIADIATFPWTRSWQNQGL
EEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEEECCCCCCHHHCCE
QIDDYPNVKRWHEEIAARPAVQRGVEVLASARVPLMDDKAKEVLFGATQYAKH
EECCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIDVYSWATPNGHKIHIMLEETGLEYTAHPINIGAGDQFARDFLAISPNNKIPAIVDTEG
CCCEEECCCCCCCEEEEEEECCCCEEEECEEECCCCHHHHHHEEEECCCCCCCEEEECCC
PKRPDGAPFSLFESGAILIYLAEKTGKFLPTDPAARYETLQWLMFQMGGIGPMLGQTHHF
CCCCCCCCEEEECCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHCCCCEE
RVYAPQPIEYAINRYTNEAKRLYGVIDTQLGKTRYLAGNDYTIADIATFPWTRSWQNQGL
EEECCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEEEEEECCCCCCHHHCCE
QIDDYPNVKRWHEEIAARPAVQRGVEVLASARVPLMDDKAKEVLFGATQYAKH
EECCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: RX; glutathione

Specific reaction: RX + glutathione = HX + R-S-glutathione

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]