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The map label for this gene is alsB [H]

Identifier: 186475996

GI number: 186475996

Start: 1389569

End: 1390525

Strand: Reverse

Name: alsB [H]

Synonym: Bphy_1237

Alternate gene names: 186475996

Gene position: 1390525-1389569 (Counterclockwise)

Preceding gene: 186475998

Following gene: 186475995

Centisome position: 39.97

GC content: 60.71

Gene sequence:

>957_bases
ATGAACCAACGCATTCGTCGCCGCGTGTTGAGCGCTGCCGTGGTATTTACGGCCGCTGCCGCGATGCCGTTTTCGTCCGC
CTGGGCCCAGAGTGCGAAGAAACCCAAGGTCGCGCTGGTGATGAAGTCGCTCGCCAACGAGTTCTTTCTCACGATGGAAA
CGGGCGCGAAGGATTACCAGAAGCACAACGCCAGCCAGTTCGACCTGATCACGAACGGCATCAAGGATGAAACCGACACG
GCGAACCAGATTCGCATCGTCGAGCAGATGATCGTGTCGAAGGTCGACGCGATCGTGCTCGCGCCGGCCGATTCGAAGGC
GCTCGTGCCCGTTGTCAAGAAGGCCGTCGACGCCGGCATCATCGTTGTGAATATCGACAACCGGCTCGATCCCGATGTGC
TGAAGTCGAAGGATCTGAACGTGCCGTTTGTCGGACCGGACAACGCGAAGGGCGCCGAAAAGGTCGGCGACTATCTCGCC
AGGAAGCTGAAATCGGGCGACGAAGTCGGGATCATCGAAGGGGTATCCACCACGACCAACGCGCAGCAACGCACGGCGGG
CTTCAAGGCGGCGATGCAGAAGGTCGGCGCGAAGATCGACACGGTACAGTCGGGCGAATGGGAAATCGACAAGGGCAATG
CGATCGCATCCTCAATGCTCAATGCATATCCCAACATCAAGGCGCTCCTGTGCGGCAACGACAATATGGCGATCGGCGCG
GTGTCGGCGGTACGCGCGGCCGGCAAGCAGGGCAAGGTTTACGTGGTTGGCTACGACAACATCAACGCGATCAAGCCGAT
GCTGAAGGACGGCCGCGTGCTCGCCACGGCTGACCAGTACGCGGCCAAGCAGGCCGTGTTCGGCATCGACGTGGCGCTCA
AGGGCATCGCCGAGCACAAGAAGCAGGCGGACCTGTCGGGCGTCGTCGAAACGCCTGTCGATCTCGTCACGAAGTAA

Upstream 100 bases:

>100_bases
CGTTGACATCGCGAACAGGCGCCGCGGAGAATAGCGCTCGCAAACGTTTGCGCACAATCAAAAAGCGGGCTTGTCCGAGC
CCAACAACTGGAGATACTGC

Downstream 100 bases:

>100_bases
CAGCCGCACGGCGGCGGGTGGCGTCGCACAGGCGCCGCTCGCCGCCGCGCGAATCCTCATCAATCGCTCAAGAAATCCGC
CGCGCCGCCGTTATGCCAGA

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm]; D-allose [Cytoplasm] [C]

Alternate protein names: ALBP [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MNQRIRRRVLSAAVVFTAAAAMPFSSAWAQSAKKPKVALVMKSLANEFFLTMETGAKDYQKHNASQFDLITNGIKDETDT
ANQIRIVEQMIVSKVDAIVLAPADSKALVPVVKKAVDAGIIVVNIDNRLDPDVLKSKDLNVPFVGPDNAKGAEKVGDYLA
RKLKSGDEVGIIEGVSTTTNAQQRTAGFKAAMQKVGAKIDTVQSGEWEIDKGNAIASSMLNAYPNIKALLCGNDNMAIGA
VSAVRAAGKQGKVYVVGYDNINAIKPMLKDGRVLATADQYAAKQAVFGIDVALKGIAEHKKQADLSGVVETPVDLVTK

Sequences:

>Translated_318_residues
MNQRIRRRVLSAAVVFTAAAAMPFSSAWAQSAKKPKVALVMKSLANEFFLTMETGAKDYQKHNASQFDLITNGIKDETDT
ANQIRIVEQMIVSKVDAIVLAPADSKALVPVVKKAVDAGIIVVNIDNRLDPDVLKSKDLNVPFVGPDNAKGAEKVGDYLA
RKLKSGDEVGIIEGVSTTTNAQQRTAGFKAAMQKVGAKIDTVQSGEWEIDKGNAIASSMLNAYPNIKALLCGNDNMAIGA
VSAVRAAGKQGKVYVVGYDNINAIKPMLKDGRVLATADQYAAKQAVFGIDVALKGIAEHKKQADLSGVVETPVDLVTK
>Mature_318_residues
MNQRIRRRVLSAAVVFTAAAAMPFSSAWAQSAKKPKVALVMKSLANEFFLTMETGAKDYQKHNASQFDLITNGIKDETDT
ANQIRIVEQMIVSKVDAIVLAPADSKALVPVVKKAVDAGIIVVNIDNRLDPDVLKSKDLNVPFVGPDNAKGAEKVGDYLA
RKLKSGDEVGIIEGVSTTTNAQQRTAGFKAAMQKVGAKIDTVQSGEWEIDKGNAIASSMLNAYPNIKALLCGNDNMAIGA
VSAVRAAGKQGKVYVVGYDNINAIKPMLKDGRVLATADQYAAKQAVFGIDVALKGIAEHKKQADLSGVVETPVDLVTK

Specific function: Part of the binding-protein-dependent transport system AlsBAC for D-allose [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790526, Length=258, Percent_Identity=35.6589147286822, Blast_Score=124, Evalue=1e-29,
Organism=Escherichia coli, GI1790192, Length=291, Percent_Identity=28.1786941580756, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1790674, Length=205, Percent_Identity=32.1951219512195, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1788898, Length=307, Percent_Identity=24.7557003257329, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 33855; Mature: 33855

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQRIRRRVLSAAVVFTAAAAMPFSSAWAQSAKKPKVALVMKSLANEFFLTMETGAKDYQ
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHEEEEEECCCHHHH
KHNASQFDLITNGIKDETDTANQIRIVEQMIVSKVDAIVLAPADSKALVPVVKKAVDAGI
HCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCEEEECCCCCCHHHHHHHHHHCCCE
IVVNIDNRLDPDVLKSKDLNVPFVGPDNAKGAEKVGDYLARKLKSGDEVGIIEGVSTTTN
EEEEECCCCCHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
AQQRTAGFKAAMQKVGAKIDTVQSGEWEIDKGNAIASSMLNAYPNIKALLCGNDNMAIGA
HHHHHHHHHHHHHHHCCCEEECCCCCEEECCCCHHHHHHHHCCCCCEEEEECCCCCEEHH
VSAVRAAGKQGKVYVVGYDNINAIKPMLKDGRVLATADQYAAKQAVFGIDVALKGIAEHK
HHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
KQADLSGVVETPVDLVTK
HHHHHHHHHHCCHHHHCC
>Mature Secondary Structure
MNQRIRRRVLSAAVVFTAAAAMPFSSAWAQSAKKPKVALVMKSLANEFFLTMETGAKDYQ
CCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHEEEEEECCCHHHH
KHNASQFDLITNGIKDETDTANQIRIVEQMIVSKVDAIVLAPADSKALVPVVKKAVDAGI
HCCCCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCEEEECCCCCCHHHHHHHHHHCCCE
IVVNIDNRLDPDVLKSKDLNVPFVGPDNAKGAEKVGDYLARKLKSGDEVGIIEGVSTTTN
EEEEECCCCCHHHHHCCCCCCCEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCC
AQQRTAGFKAAMQKVGAKIDTVQSGEWEIDKGNAIASSMLNAYPNIKALLCGNDNMAIGA
HHHHHHHHHHHHHHHCCCEEECCCCCEEECCCCHHHHHHHHCCCCCEEEEECCCCCEEHH
VSAVRAAGKQGKVYVVGYDNINAIKPMLKDGRVLATADQYAAKQAVFGIDVALKGIAEHK
HHHHHHCCCCCCEEEEECCCCHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
KQADLSGVVETPVDLVTK
HHHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; H2O; ribose [Periplasm]; D-allose [Periplasm]; ATP [C]

Specific reaction: ATP + H2O + ribose [Periplasm] = ADP + phosphate + ribose [Cytoplasm] D-allose [Periplasm] + ATP + H2O = D-allose [Cytoplasm] + ADP + phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 8576032; 9401019; 10064713 [H]