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The map label for this gene is cls [H]

Identifier: 186475976

GI number: 186475976

Start: 1366336

End: 1367598

Strand: Reverse

Name: cls [H]

Synonym: Bphy_1216

Alternate gene names: 186475976

Gene position: 1367598-1366336 (Counterclockwise)

Preceding gene: 186475977

Following gene: 186475969

Centisome position: 39.31

GC content: 61.68

Gene sequence:

>1263_bases
ATGCTGACGATCCTGCTCACAGCCGTCGCTACGCTCGTCGTCGTGCTCGTCGTCGCCAATCTGTCGACGGGCGAGAAGAA
GATCGAGCACAAGATCGAGCGCCTGTACGGCAGCGACGATCCGCAATTCATTCGCTCGATGGGGCTGCTGCTCGGTCCGC
CTGTCACGTCCGGCAATCGCTTCGAGGTGCTGGTCAACGGCGACGAGATTTTTCCGTCGATGCTCGACGGCATCGCGAGC
GCGCGCAAAACCATCACGTTCGAAACGTTCATCTACTGGTCCGGCGCGATTGGGGAACAGCTTGCGCTGGCTTTGTCGGA
GAAGGCACAAGCGGGCATTTCGGTGCATGTGCTGCTCGACTGGCTCGGTTCGCAAAAGATGGACCGGCGCTATCTCCAGA
TGCTCAAAAATGCAGGCGCCGAAGTGATCCAGTATCACAAGCCGCATTGGACCGGGCTCGGCCGCATGAACGACCGCACT
CACCGCAAACTGCTCGTGATCGACGGGCGCATCGGCTTCACGGGCGGCGTCGGCATCGCGCAGGAGTGGACGGGGCATGC
ACAGGACGACATGCACTGGCGCGATACGCATTTCCGCGTGACGGGCCCCGTGGTCGGCCACATGCAAGCTGTCTTCATGG
ACAACTGGATCAAGGCGACGGGCAACGTATTGCACGGCCCCGACTATTTTCCTGAACCGTGCGAGGCGGGCGACGGTCGC
GCGCACATGTTCAGCAGTTCCCCTTCGGGCGGCAGCGACGACATGCAGCTGATGTATCTGATGGCGATCACGGCGGCGAC
GCACTCGATACATCTTGCCAGCGCCTATTTCGTGCCGGACAGACTGACGATCAACGCGATCATCGAAGCGGCGAGGCGCG
GTGTCGACGTGAAGATCCTGACGCCGGGCCGGCACATCGACACGCATACCGTGCGCGAAGCGTCGCGCGCCTGCTGGGGA
AGACTGCTGGAAGCGGGTGCGCAGATGTTCGAGTATCAGCCGACGATGTTTCACGTCAAGCTGCTCGTCGTGGACGAATT
CCTCGTGTCGGTCGGCTCGACCAATTTCGACATGCGCTCGTTCAAGCTGAACGACGAAGCCAACCTCAATATCTACGACC
CGGCGTTCGCCCGCCGTCAGACGCAGATCTTTGCCGACGACCTCGCGAAGTCGAAACGCGTGACGCTCGAAGAATGGAAC
GGGCGGCCGTGGACGGAGAAGCTCCTCGACCGCGCGGTCGCCTTGCTCGATCCGCAGCTTTGA

Upstream 100 bases:

>100_bases
AACAATCCGGACTGGTAACGCATCGCGGCGGCGCGCGCGACGAACCGCCAGCCAGATGCAGCGCGCGCCATTCCCTCACT
GACACCGCTGAAAGCGGCGC

Downstream 100 bases:

>100_bases
GCGGCAAGCGCGTCGGCGTGCCGCTCATTGCGTCACTGCCTTGCATACGCGGCGCGCATGGGTGGTGCGTGCCCAGGCGG
ATCGCCTGCGGGCGGCTCCT

Product: phospholipase D/transphosphatidylase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MLTILLTAVATLVVVLVVANLSTGEKKIEHKIERLYGSDDPQFIRSMGLLLGPPVTSGNRFEVLVNGDEIFPSMLDGIAS
ARKTITFETFIYWSGAIGEQLALALSEKAQAGISVHVLLDWLGSQKMDRRYLQMLKNAGAEVIQYHKPHWTGLGRMNDRT
HRKLLVIDGRIGFTGGVGIAQEWTGHAQDDMHWRDTHFRVTGPVVGHMQAVFMDNWIKATGNVLHGPDYFPEPCEAGDGR
AHMFSSSPSGGSDDMQLMYLMAITAATHSIHLASAYFVPDRLTINAIIEAARRGVDVKILTPGRHIDTHTVREASRACWG
RLLEAGAQMFEYQPTMFHVKLLVVDEFLVSVGSTNFDMRSFKLNDEANLNIYDPAFARRQTQIFADDLAKSKRVTLEEWN
GRPWTEKLLDRAVALLDPQL

Sequences:

>Translated_420_residues
MLTILLTAVATLVVVLVVANLSTGEKKIEHKIERLYGSDDPQFIRSMGLLLGPPVTSGNRFEVLVNGDEIFPSMLDGIAS
ARKTITFETFIYWSGAIGEQLALALSEKAQAGISVHVLLDWLGSQKMDRRYLQMLKNAGAEVIQYHKPHWTGLGRMNDRT
HRKLLVIDGRIGFTGGVGIAQEWTGHAQDDMHWRDTHFRVTGPVVGHMQAVFMDNWIKATGNVLHGPDYFPEPCEAGDGR
AHMFSSSPSGGSDDMQLMYLMAITAATHSIHLASAYFVPDRLTINAIIEAARRGVDVKILTPGRHIDTHTVREASRACWG
RLLEAGAQMFEYQPTMFHVKLLVVDEFLVSVGSTNFDMRSFKLNDEANLNIYDPAFARRQTQIFADDLAKSKRVTLEEWN
GRPWTEKLLDRAVALLDPQL
>Mature_420_residues
MLTILLTAVATLVVVLVVANLSTGEKKIEHKIERLYGSDDPQFIRSMGLLLGPPVTSGNRFEVLVNGDEIFPSMLDGIAS
ARKTITFETFIYWSGAIGEQLALALSEKAQAGISVHVLLDWLGSQKMDRRYLQMLKNAGAEVIQYHKPHWTGLGRMNDRT
HRKLLVIDGRIGFTGGVGIAQEWTGHAQDDMHWRDTHFRVTGPVVGHMQAVFMDNWIKATGNVLHGPDYFPEPCEAGDGR
AHMFSSSPSGGSDDMQLMYLMAITAATHSIHLASAYFVPDRLTINAIIEAARRGVDVKILTPGRHIDTHTVREASRACWG
RLLEAGAQMFEYQPTMFHVKLLVVDEFLVSVGSTNFDMRSFKLNDEANLNIYDPAFARRQTQIFADDLAKSKRVTLEEWN
GRPWTEKLLDRAVALLDPQL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=372, Percent_Identity=30.1075268817204, Blast_Score=165, Evalue=6e-42,
Organism=Escherichia coli, GI1787007, Length=337, Percent_Identity=32.0474777448071, Blast_Score=154, Evalue=8e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 46871; Mature: 46871

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTILLTAVATLVVVLVVANLSTGEKKIEHKIERLYGSDDPQFIRSMGLLLGPPVTSGNR
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCE
FEVLVNGDEIFPSMLDGIASARKTITFETFIYWSGAIGEQLALALSEKAQAGISVHVLLD
EEEEECCHHHHHHHHHHHHHHHHEEEEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEHH
WLGSQKMDRRYLQMLKNAGAEVIQYHKPHWTGLGRMNDRTHRKLLVIDGRIGFTGGVGIA
HHCCCHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCH
QEWTGHAQDDMHWRDTHFRVTGPVVGHMQAVFMDNWIKATGNVLHGPDYFPEPCEAGDGR
HHCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
AHMFSSSPSGGSDDMQLMYLMAITAATHSIHLASAYFVPDRLTINAIIEAARRGVDVKIL
EEEECCCCCCCCCHHHHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHCCCEEEEE
TPGRHIDTHTVREASRACWGRLLEAGAQMFEYQPTMFHVKLLVVDEFLVSVGSTNFDMRS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHCCCCCCCEEE
FKLNDEANLNIYDPAFARRQTQIFADDLAKSKRVTLEEWNGRPWTEKLLDRAVALLDPQL
EEECCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLTILLTAVATLVVVLVVANLSTGEKKIEHKIERLYGSDDPQFIRSMGLLLGPPVTSGNR
CHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCE
FEVLVNGDEIFPSMLDGIASARKTITFETFIYWSGAIGEQLALALSEKAQAGISVHVLLD
EEEEECCHHHHHHHHHHHHHHHHEEEEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEHH
WLGSQKMDRRYLQMLKNAGAEVIQYHKPHWTGLGRMNDRTHRKLLVIDGRIGFTGGVGIA
HHCCCHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCH
QEWTGHAQDDMHWRDTHFRVTGPVVGHMQAVFMDNWIKATGNVLHGPDYFPEPCEAGDGR
HHCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
AHMFSSSPSGGSDDMQLMYLMAITAATHSIHLASAYFVPDRLTINAIIEAARRGVDVKIL
EEEECCCCCCCCCHHHHHHHHHHHHHHHEEEEEEEEECCCCHHHHHHHHHHHCCCEEEEE
TPGRHIDTHTVREASRACWGRLLEAGAQMFEYQPTMFHVKLLVVDEFLVSVGSTNFDMRS
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHCCCCCCCEEE
FKLNDEANLNIYDPAFARRQTQIFADDLAKSKRVTLEEWNGRPWTEKLLDRAVALLDPQL
EEECCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA