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The map label for this gene is acrA [H]

Identifier: 183222732

GI number: 183222732

Start: 3509393

End: 3510505

Strand: Direct

Name: acrA [H]

Synonym: LEPBI_I3388

Alternate gene names: 183222732

Gene position: 3509393-3510505 (Clockwise)

Preceding gene: 183222731

Following gene: 183222733

Centisome position: 97.49

GC content: 35.58

Gene sequence:

>1113_bases
ATGAATCTTTTAATCGAGATCAAGCATTCTTTTTTGACTAGAATACTTTATACAGTATTTTTAATTTCGTTTCTTTTCCT
GATCAATTGTCATGGACAAAATCATGAAGACAAAAATTCACTTATGGCAACATATCCTTGGAAACAGGATGTAACAATTG
ATAAAAACTATGTGGCACAAGTGAAAGCAATCCAACGTATCGAAGTAAGAGCCTTCGAAAAGGGATATCTAACACAGATT
TATATGGATGAAGGAAGGGTTGTCAAAAAAGGACAAAAGTTATTCCAAGTGATGCCAATGCTTGTTAATGCACAATACGA
CAAAGCCAAAGCTGAGTATGAATCCACACAAATTGAATACGAAAACACTGAGAAATTATTTAAAGAAAATGTAGTTTCTC
AAACTGAACTTTCACTCATCAAAGCACGTTTAAAGAAAAATAAAGCCGCCATGGAATTGGCACAAGTCCATTTGAATTTA
GCAACTGTGACAGCACCTTTTACGGGAATCACGGATCGATTCCAAGTCCGGCTCGGGAGCTTGGTGGAAGAAGGAACTTT
ATTAACGACCATCTCTGATATTTCCAAACTTTGGGTCTATTTCAATGTTTCGGAAAAAGATTATTTGAATTTTTCGAATG
CAAGAAGATCCGGAGACAAACCATTAAAGGTAAAATTTCTGATGGCAAATGGAGAATTTTTCCAACAGGAAGGAATCGCC
GACACAATTGAAGGTGAATTCGATAGTGAGACAGGCACCATTCCATTTCGTGCCACGTTTCCAAATCCAAACAGACTATT
ACGTCATGGAGAAACCGGAAATGTTGTGGTAAAAGAAAGTTTAAAAGATGCTCTTCTCATTCCTCAAAAAGCAACATTCG
AAGTATTAGATAAACGTTATGTTTATACCATTTCTAGTAAAGGAATTCTAAAAACAAAAGAAATTAAAATCTCTAATGAA
ATCCCACACTTGTTTGTTGTCGAATCCGGTATTACTGAAACCGACATCATCCTTTTAGAAGGGCTTGGTAAAGTACATGA
TGGTGATACTATAAAGTTTAAAATCGAAGCTCGTGAAAATATCATGAAAAGTTTCGAGTTATCGGCTCACTAG

Upstream 100 bases:

>100_bases
CACTTAACATAAAATTTCATTCAAAAGAAATCGCAGGATTTAATGCGATCGGTGATATTTAAAATTTTAACTAACTTTCG
TTAGTTCTTTTGGGGACACA

Downstream 100 bases:

>100_bases
GAGATCATTGTATGTTTACAAAATTTCTCCAGAGACCAGTTCTCGCTATCGTTATTTCCGTATTAGTTGTGTTTGTTGGT
TTAATATCAATTAAAAACAT

Product: multidrug resistance protein RND family putative acriflavine resistance protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MNLLIEIKHSFLTRILYTVFLISFLFLINCHGQNHEDKNSLMATYPWKQDVTIDKNYVAQVKAIQRIEVRAFEKGYLTQI
YMDEGRVVKKGQKLFQVMPMLVNAQYDKAKAEYESTQIEYENTEKLFKENVVSQTELSLIKARLKKNKAAMELAQVHLNL
ATVTAPFTGITDRFQVRLGSLVEEGTLLTTISDISKLWVYFNVSEKDYLNFSNARRSGDKPLKVKFLMANGEFFQQEGIA
DTIEGEFDSETGTIPFRATFPNPNRLLRHGETGNVVVKESLKDALLIPQKATFEVLDKRYVYTISSKGILKTKEIKISNE
IPHLFVVESGITETDIILLEGLGKVHDGDTIKFKIEARENIMKSFELSAH

Sequences:

>Translated_370_residues
MNLLIEIKHSFLTRILYTVFLISFLFLINCHGQNHEDKNSLMATYPWKQDVTIDKNYVAQVKAIQRIEVRAFEKGYLTQI
YMDEGRVVKKGQKLFQVMPMLVNAQYDKAKAEYESTQIEYENTEKLFKENVVSQTELSLIKARLKKNKAAMELAQVHLNL
ATVTAPFTGITDRFQVRLGSLVEEGTLLTTISDISKLWVYFNVSEKDYLNFSNARRSGDKPLKVKFLMANGEFFQQEGIA
DTIEGEFDSETGTIPFRATFPNPNRLLRHGETGNVVVKESLKDALLIPQKATFEVLDKRYVYTISSKGILKTKEIKISNE
IPHLFVVESGITETDIILLEGLGKVHDGDTIKFKIEARENIMKSFELSAH
>Mature_370_residues
MNLLIEIKHSFLTRILYTVFLISFLFLINCHGQNHEDKNSLMATYPWKQDVTIDKNYVAQVKAIQRIEVRAFEKGYLTQI
YMDEGRVVKKGQKLFQVMPMLVNAQYDKAKAEYESTQIEYENTEKLFKENVVSQTELSLIKARLKKNKAAMELAQVHLNL
ATVTAPFTGITDRFQVRLGSLVEEGTLLTTISDISKLWVYFNVSEKDYLNFSNARRSGDKPLKVKFLMANGEFFQQEGIA
DTIEGEFDSETGTIPFRATFPNPNRLLRHGETGNVVVKESLKDALLIPQKATFEVLDKRYVYTISSKGILKTKEIKISNE
IPHLFVVESGITETDIILLEGLGKVHDGDTIKFKIEARENIMKSFELSAH

Specific function: The periplasmic linker component of an organic solvent efflux pump. Involved in export of a number of organic solvents, including toluene and styrene. This is the most important solvent efflux pump in this strain, although it can export AMP and some antib

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789929, Length=316, Percent_Identity=27.2151898734177, Blast_Score=109, Evalue=3e-25,
Organism=Escherichia coli, GI1789665, Length=369, Percent_Identity=24.6612466124661, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786668, Length=378, Percent_Identity=24.6031746031746, Blast_Score=93, Evalue=3e-20,
Organism=Escherichia coli, GI87082036, Length=315, Percent_Identity=23.4920634920635, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI87081796, Length=315, Percent_Identity=24.4444444444444, Blast_Score=68, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42277; Mature: 42277

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLIEIKHSFLTRILYTVFLISFLFLINCHGQNHEDKNSLMATYPWKQDVTIDKNYVAQ
CCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCEECHHHHHH
VKAIQRIEVRAFEKGYLTQIYMDEGRVVKKGQKLFQVMPMLVNAQYDKAKAEYESTQIEY
HHHHHHHHHHEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCEEEE
ENTEKLFKENVVSQTELSLIKARLKKNKAAMELAQVHLNLATVTAPFTGITDRFQVRLGS
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHH
LVEEGTLLTTISDISKLWVYFNVSEKDYLNFSNARRSGDKPLKVKFLMANGEFFQQEGIA
HHHCCEEEEEHHHHCEEEEEEECCCCHHCCCHHHCCCCCCCEEEEEEEECCCHHHHCCCC
DTIEGEFDSETGTIPFRATFPNPNRLLRHGETGNVVVKESLKDALLIPQKATFEVLDKRY
HHCCCCCCCCCCCEEEEEECCCHHHHHCCCCCCCEEEECCCHHHEECCCCHHHHHHCCEE
VYTISSKGILKTKEIKISNEIPHLFVVESGITETDIILLEGLGKVHDGDTIKFKIEAREN
EEEECCCCCEEEEEEEECCCCCEEEEEECCCCCCEEEEEECCCCCCCCCEEEEEEECHHH
IMKSFELSAH
HHHHHCCCCC
>Mature Secondary Structure
MNLLIEIKHSFLTRILYTVFLISFLFLINCHGQNHEDKNSLMATYPWKQDVTIDKNYVAQ
CCEEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCEECHHHHHH
VKAIQRIEVRAFEKGYLTQIYMDEGRVVKKGQKLFQVMPMLVNAQYDKAKAEYESTQIEY
HHHHHHHHHHEECCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHCEEEE
ENTEKLFKENVVSQTELSLIKARLKKNKAAMELAQVHLNLATVTAPFTGITDRFQVRLGS
CHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCHHHHHHHHH
LVEEGTLLTTISDISKLWVYFNVSEKDYLNFSNARRSGDKPLKVKFLMANGEFFQQEGIA
HHHCCEEEEEHHHHCEEEEEEECCCCHHCCCHHHCCCCCCCEEEEEEEECCCHHHHCCCC
DTIEGEFDSETGTIPFRATFPNPNRLLRHGETGNVVVKESLKDALLIPQKATFEVLDKRY
HHCCCCCCCCCCCEEEEEECCCHHHHHCCCCCCCEEEECCCHHHEECCCCHHHHHHCCEE
VYTISSKGILKTKEIKISNEIPHLFVVESGITETDIILLEGLGKVHDGDTIKFKIEAREN
EEEECCCCCEEEEEEEECCCCCEEEEEECCCCCCEEEEEECCCCCCCCCEEEEEEECHHH
IMKSFELSAH
HHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11395460 [H]