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The map label for this gene is ydfG [C]

Identifier: 183222600

GI number: 183222600

Start: 3369340

End: 3370119

Strand: Direct

Name: ydfG [C]

Synonym: LEPBI_I3255

Alternate gene names: 183222600

Gene position: 3369340-3370119 (Clockwise)

Preceding gene: 183222598

Following gene: 183222601

Centisome position: 93.6

GC content: 37.69

Gene sequence:

>780_bases
ATGAAATATGCTTTAATCACAGGTGCATCTACGGGACTAGGAAAAGATTTTGCCCATTCGTTGGCAAAACTAGGTTATAC
TCCAATATTGGTAGCTAGGAATGCTGACAGACTCAAAAGTCTTGCGGCCGAAATCAAACAGAAGTATGGTTTAGACGGCA
TTGTCATCGCAGAAGATTTATCAGTTCCAAATGCTGCTGAAAAAATTTATAAATCAGTCAAAAAGTTAAAAGTACAAGTC
AGTTGTTTGGTTAATAATGCTGGGTACGGTTTAAATGGAGAATTTCACAAAAATTCATTTTCAGAAGAATCAAAGATGAT
CCAACTGAATGTCACTACTTTGGCGGAATTATGCCATTTGTTTTTACAAGACATGGTGACAAATAAGGAAGGATACATTC
TCAACGTGGCTTCTACTGCTGCCTTCCAACCAGGACCATTGATGACAAACTACTATGCCACCAAAGCATACGTACTCTCG
TTAAGCGAAGGTTTAGCGGAAGAAGTTCGAGAGTATGGTGTAACCGTTTCCTGCCTATGCCCTGGTCCCACTAAAACAGA
ATTTTTTGAACGAGCAAAAATGTCAGATAGTAAGTTAGTGAAATCTTCGATATTAGCAATGAACTCCCAAGATGTTGTGG
ATATTGGTTTAAAGGCTTTATTTGGAAAGAAAGTCGTAAAAATTCCAGGTTTTTTGAACTTTGTATTAGCAGAGTCAATC
CGTGTGACGCCAAGAGCAATGATTCGTAAAATAGCAAAGTATCTAAATAAAGTTGGTTGA

Upstream 100 bases:

>100_bases
GAGGTTGGCTCATAATACGAATATTGACCAGCTAGTCAAAATTGCCAAAACAATTTATTTAAGAATTGCCAAGTTTTCTA
AACTGAAATAAATTTCCTTT

Downstream 100 bases:

>100_bases
TTTGACAAAAAATTTATTCCCGTTTTCAAATCGTTTTTCTTCGTTAGGGGAAATCCAATCTGAAAATTCTATTCATAAAA
CTAAATCAGATTTAATAGAA

Product: SDR family dehydrogenase/reductase

Products: 3-oxoacyl-[acyl-carrier protein]; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MKYALITGASTGLGKDFAHSLAKLGYTPILVARNADRLKSLAAEIKQKYGLDGIVIAEDLSVPNAAEKIYKSVKKLKVQV
SCLVNNAGYGLNGEFHKNSFSEESKMIQLNVTTLAELCHLFLQDMVTNKEGYILNVASTAAFQPGPLMTNYYATKAYVLS
LSEGLAEEVREYGVTVSCLCPGPTKTEFFERAKMSDSKLVKSSILAMNSQDVVDIGLKALFGKKVVKIPGFLNFVLAESI
RVTPRAMIRKIAKYLNKVG

Sequences:

>Translated_259_residues
MKYALITGASTGLGKDFAHSLAKLGYTPILVARNADRLKSLAAEIKQKYGLDGIVIAEDLSVPNAAEKIYKSVKKLKVQV
SCLVNNAGYGLNGEFHKNSFSEESKMIQLNVTTLAELCHLFLQDMVTNKEGYILNVASTAAFQPGPLMTNYYATKAYVLS
LSEGLAEEVREYGVTVSCLCPGPTKTEFFERAKMSDSKLVKSSILAMNSQDVVDIGLKALFGKKVVKIPGFLNFVLAESI
RVTPRAMIRKIAKYLNKVG
>Mature_259_residues
MKYALITGASTGLGKDFAHSLAKLGYTPILVARNADRLKSLAAEIKQKYGLDGIVIAEDLSVPNAAEKIYKSVKKLKVQV
SCLVNNAGYGLNGEFHKNSFSEESKMIQLNVTTLAELCHLFLQDMVTNKEGYILNVASTAAFQPGPLMTNYYATKAYVLS
LSEGLAEEVREYGVTVSCLCPGPTKTEFFERAKMSDSKLVKSSILAMNSQDVVDIGLKALFGKKVVKIPGFLNFVLAESI
RVTPRAMIRKIAKYLNKVG

Specific function: Unknown

COG id: COG0300

COG function: function code R; Short-chain dehydrogenases of various substrate specificities

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI7705855, Length=267, Percent_Identity=31.0861423220974, Blast_Score=109, Evalue=3e-24,
Organism=Homo sapiens, GI4557649, Length=187, Percent_Identity=32.0855614973262, Blast_Score=101, Evalue=7e-22,
Organism=Homo sapiens, GI226371731, Length=184, Percent_Identity=30.9782608695652, Blast_Score=88, Evalue=7e-18,
Organism=Homo sapiens, GI7706318, Length=191, Percent_Identity=30.3664921465969, Blast_Score=84, Evalue=1e-16,
Organism=Homo sapiens, GI109715829, Length=195, Percent_Identity=30.2564102564103, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI142976729, Length=197, Percent_Identity=31.9796954314721, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI210032110, Length=239, Percent_Identity=25.9414225941423, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI40254992, Length=187, Percent_Identity=24.0641711229947, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1787820, Length=246, Percent_Identity=31.3008130081301, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1787335, Length=205, Percent_Identity=28.780487804878, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI87082100, Length=190, Percent_Identity=30, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1786701, Length=199, Percent_Identity=27.1356783919598, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87082160, Length=225, Percent_Identity=26.6666666666667, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI2367175, Length=197, Percent_Identity=30.4568527918782, Blast_Score=64, Evalue=7e-12,
Organism=Escherichia coli, GI1790717, Length=191, Percent_Identity=28.2722513089005, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI25152911, Length=185, Percent_Identity=31.8918918918919, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17562906, Length=200, Percent_Identity=30, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71994600, Length=199, Percent_Identity=31.1557788944724, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71994604, Length=199, Percent_Identity=29.1457286432161, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17560220, Length=198, Percent_Identity=30.8080808080808, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17562908, Length=204, Percent_Identity=30.8823529411765, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI212646214, Length=202, Percent_Identity=28.2178217821782, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17557780, Length=197, Percent_Identity=29.4416243654822, Blast_Score=67, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI17562910, Length=225, Percent_Identity=28, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI115534694, Length=197, Percent_Identity=30.9644670050761, Blast_Score=65, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17531453, Length=201, Percent_Identity=27.8606965174129, Blast_Score=65, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17508895, Length=192, Percent_Identity=28.6458333333333, Blast_Score=64, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6319635, Length=232, Percent_Identity=26.7241379310345, Blast_Score=80, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24640442, Length=227, Percent_Identity=30.8370044052863, Blast_Score=112, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24584752, Length=207, Percent_Identity=31.8840579710145, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI161076933, Length=183, Percent_Identity=30.6010928961749, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24584748, Length=182, Percent_Identity=30.7692307692308, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24584744, Length=184, Percent_Identity=29.3478260869565, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI28574402, Length=184, Percent_Identity=29.3478260869565, Blast_Score=91, Evalue=9e-19,
Organism=Drosophila melanogaster, GI21357041, Length=200, Percent_Identity=30, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI28571526, Length=200, Percent_Identity=29.5, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24644339, Length=193, Percent_Identity=29.5336787564767, Blast_Score=77, Evalue=9e-15,
Organism=Drosophila melanogaster, GI21358495, Length=192, Percent_Identity=28.125, Blast_Score=72, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24580925, Length=198, Percent_Identity=29.7979797979798, Blast_Score=72, Evalue=5e-13,
Organism=Drosophila melanogaster, GI24639444, Length=185, Percent_Identity=29.7297297297297, Blast_Score=64, Evalue=8e-11,

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100

Molecular weight: Translated: 28272; Mature: 28272

Theoretical pI: Translated: 9.72; Mature: 9.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKYALITGASTGLGKDFAHSLAKLGYTPILVARNADRLKSLAAEIKQKYGLDGIVIAEDL
CCEEEEECCCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEEECC
SVPNAAEKIYKSVKKLKVQVSCLVNNAGYGLNGEFHKNSFSEESKMIQLNVTTLAELCHL
CCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHH
FLQDMVTNKEGYILNVASTAAFQPGPLMTNYYATKAYVLSLSEGLAEEVREYGVTVSCLC
HHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEC
PGPTKTEFFERAKMSDSKLVKSSILAMNSQDVVDIGLKALFGKKVVKIPGFLNFVLAESI
CCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHEECCHHHHHHHHHHC
RVTPRAMIRKIAKYLNKVG
CCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKYALITGASTGLGKDFAHSLAKLGYTPILVARNADRLKSLAAEIKQKYGLDGIVIAEDL
CCEEEEECCCCCCCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCEEEEECC
SVPNAAEKIYKSVKKLKVQVSCLVNNAGYGLNGEFHKNSFSEESKMIQLNVTTLAELCHL
CCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHH
FLQDMVTNKEGYILNVASTAAFQPGPLMTNYYATKAYVLSLSEGLAEEVREYGVTVSCLC
HHHHHHCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEC
PGPTKTEFFERAKMSDSKLVKSSILAMNSQDVVDIGLKALFGKKVVKIPGFLNFVLAESI
CCCCHHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHEECCHHHHHHHHHHC
RVTPRAMIRKIAKYLNKVG
CCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (3R)-3-hydroxyacyl-[acyl-carrier protein]; NADP+

Specific reaction: (3R)-3-hydroxyacyl-[acyl-carrier protein] + NADP+ = 3-oxoacyl-[acyl-carrier protein] + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12950922 [H]