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The map label for this gene is dfrA [H]

Identifier: 183222524

GI number: 183222524

Start: 3292542

End: 3293618

Strand: Direct

Name: dfrA [H]

Synonym: LEPBI_I3179

Alternate gene names: 183222524

Gene position: 3292542-3293618 (Clockwise)

Preceding gene: 183222520

Following gene: 183222526

Centisome position: 91.47

GC content: 37.98

Gene sequence:

>1077_bases
TTGGAATTGAGTCTAAAACTTGAAATGAAATCAATCAATAAAGATTTGCCCGTTGTTGTCACTGGAGGTTCAGGATATAT
TGCGTCTTGGATCGTAAAATATCTGTTAGAGGATGGAAAAAAAGTAAGGGCAACGGTTCGAAGCCTGAAAGATACCTCCA
AAATCCAACATCTTTTGGATTTAAAAGAAAAGTTCAAAGACAACCTAACTTTGTTTGAGGCGGACCTTATGGTCGAGGGT
AGTTTTGACAAAAGTGTGGAAGGTGCCGAACTTGTGATCCATACTGCTTCACCTTTTTTTGTGGCAGGAGTTAAGGACGC
AAAAAAACAGTTGATCGATCCCGCTTTACAAGGAACAAGAAATGTTCTTGAAACCTGCAACCGTATTTCATCCGTTAAAC
GAGTAGTTTTAACATCCAGTGTTGCTGCCATCCATGGTGACAATATCGATTCCTTAAAAGTTCCAAACCAAACCTTTACA
GAAGAACATTGGAATACAACAAGTAGTCTGACACACCAACCTTATGCTTATTCCAAAACATTAGCCGAAAAAGAAGCGTG
GGAAATGCAAAAGAAACAATCACGTTGGGATTTGGTTGTCATCAATCCTTCTTTTGTAATGGGTCCATCACTTTCCAAAC
GTTTGGATGGTACAAGTGTGGAGTTTATGAAAAATATGTTGAAAGGAATTTTCCGTACCGGTGTTCCTGATAACAAAATG
GGATTTGTTGATGTGAGAGATGTTGCAAAGGCACATATCTTGGCAGGTTTTACACCGAATGCAAAAGGAAGGCATATCAC
ATCAGCAGAAGTATTACCGATGTTAGGCATTGCCAAAATCATTAAAGAAAAATTTGGAAACAAATATTCTGTTCCGACAG
GTGTTCTTCCGAAACCACTTGTGTATTTGGTAGGTCCATTTTTTGGATTATCTTGGGGTTATACAAAAAATAATATTGGG
CAACCAATGAACTTAAACAATGAGTATAGTAAAACCGATTTATCTCTCACCTATCGTCCATTAAATGAGACAATCATCGA
TCATGTGAACCAAATGGAAGGTTCGGGTTTGTTATAA

Upstream 100 bases:

>100_bases
CGGACTCCGGAAAAGCCCGGCCCATCGGGACTCGCCCATTAAAAGAAAACAAAATGGATTTTTGTTTCTTTTATCCATAT
GGAAATGTTCACGCTAAAGA

Downstream 100 bases:

>100_bases
TTGTTTATGGAGGCGATTGCATTGGGAATGGGTGAAACTACGAAGTTTTTTTAAGATTTTTTGGTAAGATTGAATTTCCA
AGAGACTTGCGTTCCGTTTT

Product: putative dihydroflavonol-4-reductase

Products: NA

Alternate protein names: DFR; Dihydrokaempferol 4-reductase [H]

Number of amino acids: Translated: 358; Mature: 358

Protein sequence:

>358_residues
MELSLKLEMKSINKDLPVVVTGGSGYIASWIVKYLLEDGKKVRATVRSLKDTSKIQHLLDLKEKFKDNLTLFEADLMVEG
SFDKSVEGAELVIHTASPFFVAGVKDAKKQLIDPALQGTRNVLETCNRISSVKRVVLTSSVAAIHGDNIDSLKVPNQTFT
EEHWNTTSSLTHQPYAYSKTLAEKEAWEMQKKQSRWDLVVINPSFVMGPSLSKRLDGTSVEFMKNMLKGIFRTGVPDNKM
GFVDVRDVAKAHILAGFTPNAKGRHITSAEVLPMLGIAKIIKEKFGNKYSVPTGVLPKPLVYLVGPFFGLSWGYTKNNIG
QPMNLNNEYSKTDLSLTYRPLNETIIDHVNQMEGSGLL

Sequences:

>Translated_358_residues
MELSLKLEMKSINKDLPVVVTGGSGYIASWIVKYLLEDGKKVRATVRSLKDTSKIQHLLDLKEKFKDNLTLFEADLMVEG
SFDKSVEGAELVIHTASPFFVAGVKDAKKQLIDPALQGTRNVLETCNRISSVKRVVLTSSVAAIHGDNIDSLKVPNQTFT
EEHWNTTSSLTHQPYAYSKTLAEKEAWEMQKKQSRWDLVVINPSFVMGPSLSKRLDGTSVEFMKNMLKGIFRTGVPDNKM
GFVDVRDVAKAHILAGFTPNAKGRHITSAEVLPMLGIAKIIKEKFGNKYSVPTGVLPKPLVYLVGPFFGLSWGYTKNNIG
QPMNLNNEYSKTDLSLTYRPLNETIIDHVNQMEGSGLL
>Mature_358_residues
MELSLKLEMKSINKDLPVVVTGGSGYIASWIVKYLLEDGKKVRATVRSLKDTSKIQHLLDLKEKFKDNLTLFEADLMVEG
SFDKSVEGAELVIHTASPFFVAGVKDAKKQLIDPALQGTRNVLETCNRISSVKRVVLTSSVAAIHGDNIDSLKVPNQTFT
EEHWNTTSSLTHQPYAYSKTLAEKEAWEMQKKQSRWDLVVINPSFVMGPSLSKRLDGTSVEFMKNMLKGIFRTGVPDNKM
GFVDVRDVAKAHILAGFTPNAKGRHITSAEVLPMLGIAKIIKEKFGNKYSVPTGVLPKPLVYLVGPFFGLSWGYTKNNIG
QPMNLNNEYSKTDLSLTYRPLNETIIDHVNQMEGSGLL

Specific function: Galactose metabolism; third step. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dihydroflavonol-4-reductase family [H]

Homologues:

Organism=Homo sapiens, GI260763931, Length=364, Percent_Identity=25, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI4504509, Length=364, Percent_Identity=25, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI239745448, Length=302, Percent_Identity=24.8344370860927, Blast_Score=69, Evalue=5e-12,
Organism=Homo sapiens, GI310132178, Length=302, Percent_Identity=24.8344370860927, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI310113012, Length=302, Percent_Identity=24.8344370860927, Blast_Score=68, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI71987463, Length=352, Percent_Identity=32.6704545454545, Blast_Score=165, Evalue=3e-41,
Organism=Saccharomyces cerevisiae, GI6324421, Length=357, Percent_Identity=32.2128851540616, Blast_Score=131, Evalue=1e-31,
Organism=Saccharomyces cerevisiae, GI6320750, Length=355, Percent_Identity=31.830985915493, Blast_Score=127, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6321399, Length=356, Percent_Identity=30.0561797752809, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6321281, Length=364, Percent_Identity=29.9450549450549, Blast_Score=110, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR017829
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.219 [H]

Molecular weight: Translated: 39790; Mature: 39790

Theoretical pI: Translated: 9.71; Mature: 9.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELSLKLEMKSINKDLPVVVTGGSGYIASWIVKYLLEDGKKVRATVRSLKDTSKIQHLLD
CCEEEEEEHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LKEKFKDNLTLFEADLMVEGSFDKSVEGAELVIHTASPFFVAGVKDAKKQLIDPALQGTR
HHHHHHCCCEEEEEEEEEECCCCCCCCCCEEEEEECCCEEEECHHHHHHHHHHHHHHHHH
NVLETCNRISSVKRVVLTSSVAAIHGDNIDSLKVPNQTFTEEHWNTTSSLTHQPYAYSKT
HHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEECCCCHHHHHHCCCHHHCCCCCCHHHHH
LAEKEAWEMQKKQSRWDLVVINPSFVMGPSLSKRLDGTSVEFMKNMLKGIFRTGVPDNKM
HHHHHHHHHHHHCCCCCEEEECCCEEECCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCCC
GFVDVRDVAKAHILAGFTPNAKGRHITSAEVLPMLGIAKIIKEKFGNKYSVPTGVLPKPL
CCEEHHHHHHHHHHCCCCCCCCCCEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
VYLVGPFFGLSWGYTKNNIGQPMNLNNEYSKTDLSLTYRPLNETIIDHVNQMEGSGLL
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MELSLKLEMKSINKDLPVVVTGGSGYIASWIVKYLLEDGKKVRATVRSLKDTSKIQHLLD
CCEEEEEEHHHCCCCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHH
LKEKFKDNLTLFEADLMVEGSFDKSVEGAELVIHTASPFFVAGVKDAKKQLIDPALQGTR
HHHHHHCCCEEEEEEEEEECCCCCCCCCCEEEEEECCCEEEECHHHHHHHHHHHHHHHHH
NVLETCNRISSVKRVVLTSSVAAIHGDNIDSLKVPNQTFTEEHWNTTSSLTHQPYAYSKT
HHHHHHHHHHHHHHHHHHHHHHHEECCCCCCEECCCCHHHHHHCCCHHHCCCCCCHHHHH
LAEKEAWEMQKKQSRWDLVVINPSFVMGPSLSKRLDGTSVEFMKNMLKGIFRTGVPDNKM
HHHHHHHHHHHHCCCCCEEEECCCEEECCCHHHHCCCCHHHHHHHHHHHHHHCCCCCCCC
GFVDVRDVAKAHILAGFTPNAKGRHITSAEVLPMLGIAKIIKEKFGNKYSVPTGVLPKPL
CCEEHHHHHHHHHHCCCCCCCCCCEECHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
VYLVGPFFGLSWGYTKNNIGQPMNLNNEYSKTDLSLTYRPLNETIIDHVNQMEGSGLL
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]