Definition Leptospira biflexa serovar Patoc strain 'Patoc 1 (Paris)' chromosome chromosome I, complete sequence.
Accession NC_010602
Length 3,599,677

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The map label for this gene is pck

Identifier: 183222336

GI number: 183222336

Start: 3094825

End: 3096417

Strand: Reverse

Name: pck

Synonym: LEPBI_I2989

Alternate gene names: 183222336

Gene position: 3096417-3094825 (Counterclockwise)

Preceding gene: 183222343

Following gene: 183222328

Centisome position: 86.02

GC content: 43.19

Gene sequence:

>1593_bases
ATGTCAGTATCTACTAATCTAAAAGGCCTTGCTGAACTCGGCCTAAAACCGTCCGAAGTCTTCCATAACTTATCATACGA
AGAAATTTACCAGCACGAATTGAATAACAAAGAAGGTGTTACTTCTGATAATGGAACGATGATGGTGGATACCGGGATTT
TTACGGGTCGTTCTCCGAAGGACAAATACTTTGTCGATGAACCTTCTTCTCAAAACAACATTTGGTGGGGACCAGTCAAC
ACAAAGGTTTCCGAAGCGATTTTTAACGAACTCTACGCAGAAGTAACGAAATTTCTCGATAACAAAAAATTATACGTATT
TGATGGCCACGCAGGAACCAACGACGACACACGTATCTCTCTCCGTGTGGTCACAGAACGCGCTTGGCAACACCACTTCT
GCACAAACATGTTCCTTCGCCCAACCAAAGAAGAACTCGCAAAACTCGATCCAGAGTTTACCATCATCAACGCATCTGGT
TATAAAAATCCAAAATACAAAGAACACGGCCTCAACTCCGAAGTATTTGTGATCTTCCACTTAGCAAAAAAAATCTGTAT
CATCGGTGGAACAGAATACGGTGGGGAAATGAAAAAAGGGATTTTCTCTGTCATGAACTACTACCTCCCACTGAAGAATG
TCCTCACCATGCATTGTTCCGCTAACGTTGGTAAGGATGGAGACAGCGCTCTTTTCTTTGGTCTTTCTGGAACTGGAAAA
ACAACTCTTTCCACTGACCCAAATCGTAAACTCATCGGGGACGACGAACATGGTTGGGACGACAATGGAATCTTCAACAT
TGAAGGTGGATGTTACGCAAAAACCATCAACCTGGATCCAAAAACAGAACCAGAAATCTATGCAGCCATCCGTCGTGATG
CGTTACTTGAAAACGTAGTTTACGATGCGACAACAAAGAAAGTAGATTACTCTTCTGCGGCAAAAACAGAAAACACACGA
GTTTCTTACCCAATCTTCCATATCGACAACATCCAACCAGGATCGAAAGCAGGTCACCCAAACACTGTGATTTTCCTTAC
TTACGATGCATACGGTGTGCTCCCTGCGGTATCTAAACTTTCGATCGAACAAGCGATGTATCACTTCCTTTCTGGTTACA
CAGCAAAGGTAGCAGGGACTGAGCGCGGTGTGAAAGAACCACAAGCCACTTTCTCTGCTTGTTTTGGTCAGGCGTTTATG
ACTCTCCACCCAACCTACTATGCAAAGTTACTCGGTGAAAAAATGAAAAAACACCAAGTGAATGCTTACCTCATCAACAC
TGGTCTTGTGGGTGGAAAGTATGGAGTGGGAAAACGGATGAACCTTCCTGCAACTCGCCAAATCATCAATGAAATCCTGA
ACGGAAACATCGAAAAATCCGAGTTTGAAAAACACCCAGTTTTCCAAGTGTCTTTCCCGAAATCCGTAAACGGTGTGGAT
GCACATATCCTAAACCCACGAAACGCTTGGGAAAACAAAGAAGACTACGACAAAACTGCCGCTGACCTTGCGAAACAATT
TGTAGAAAACTACAAAAAGTATCTCACTGGTTCAAAGGAATTTGATTACAGCCAATACGGCCCGATTGCGTAA

Upstream 100 bases:

>100_bases
ATATTCTGCAGAAGCGTGGAGAATCTTCATTTTCTGTTATTGACACCGACTCGCAAAGTAAGAAGCTAGAAAAACACAAA
GTGAAATCGAGGAGAGATCC

Downstream 100 bases:

>100_bases
ATCGCATTCGGAATTAAAAAGCTAAGGGTTGTCGTTTCGTAGATCCGTTTGCCAGCTTTCGAAAGTTAGCTGGTAATTCA
ATTCAACAAAATAACAATCG

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MSVSTNLKGLAELGLKPSEVFHNLSYEEIYQHELNNKEGVTSDNGTMMVDTGIFTGRSPKDKYFVDEPSSQNNIWWGPVN
TKVSEAIFNELYAEVTKFLDNKKLYVFDGHAGTNDDTRISLRVVTERAWQHHFCTNMFLRPTKEELAKLDPEFTIINASG
YKNPKYKEHGLNSEVFVIFHLAKKICIIGGTEYGGEMKKGIFSVMNYYLPLKNVLTMHCSANVGKDGDSALFFGLSGTGK
TTLSTDPNRKLIGDDEHGWDDNGIFNIEGGCYAKTINLDPKTEPEIYAAIRRDALLENVVYDATTKKVDYSSAAKTENTR
VSYPIFHIDNIQPGSKAGHPNTVIFLTYDAYGVLPAVSKLSIEQAMYHFLSGYTAKVAGTERGVKEPQATFSACFGQAFM
TLHPTYYAKLLGEKMKKHQVNAYLINTGLVGGKYGVGKRMNLPATRQIINEILNGNIEKSEFEKHPVFQVSFPKSVNGVD
AHILNPRNAWENKEDYDKTAADLAKQFVENYKKYLTGSKEFDYSQYGPIA

Sequences:

>Translated_530_residues
MSVSTNLKGLAELGLKPSEVFHNLSYEEIYQHELNNKEGVTSDNGTMMVDTGIFTGRSPKDKYFVDEPSSQNNIWWGPVN
TKVSEAIFNELYAEVTKFLDNKKLYVFDGHAGTNDDTRISLRVVTERAWQHHFCTNMFLRPTKEELAKLDPEFTIINASG
YKNPKYKEHGLNSEVFVIFHLAKKICIIGGTEYGGEMKKGIFSVMNYYLPLKNVLTMHCSANVGKDGDSALFFGLSGTGK
TTLSTDPNRKLIGDDEHGWDDNGIFNIEGGCYAKTINLDPKTEPEIYAAIRRDALLENVVYDATTKKVDYSSAAKTENTR
VSYPIFHIDNIQPGSKAGHPNTVIFLTYDAYGVLPAVSKLSIEQAMYHFLSGYTAKVAGTERGVKEPQATFSACFGQAFM
TLHPTYYAKLLGEKMKKHQVNAYLINTGLVGGKYGVGKRMNLPATRQIINEILNGNIEKSEFEKHPVFQVSFPKSVNGVD
AHILNPRNAWENKEDYDKTAADLAKQFVENYKKYLTGSKEFDYSQYGPIA
>Mature_529_residues
SVSTNLKGLAELGLKPSEVFHNLSYEEIYQHELNNKEGVTSDNGTMMVDTGIFTGRSPKDKYFVDEPSSQNNIWWGPVNT
KVSEAIFNELYAEVTKFLDNKKLYVFDGHAGTNDDTRISLRVVTERAWQHHFCTNMFLRPTKEELAKLDPEFTIINASGY
KNPKYKEHGLNSEVFVIFHLAKKICIIGGTEYGGEMKKGIFSVMNYYLPLKNVLTMHCSANVGKDGDSALFFGLSGTGKT
TLSTDPNRKLIGDDEHGWDDNGIFNIEGGCYAKTINLDPKTEPEIYAAIRRDALLENVVYDATTKKVDYSSAAKTENTRV
SYPIFHIDNIQPGSKAGHPNTVIFLTYDAYGVLPAVSKLSIEQAMYHFLSGYTAKVAGTERGVKEPQATFSACFGQAFMT
LHPTYYAKLLGEKMKKHQVNAYLINTGLVGGKYGVGKRMNLPATRQIINEILNGNIEKSEFEKHPVFQVSFPKSVNGVDA
HILNPRNAWENKEDYDKTAADLAKQFVENYKKYLTGSKEFDYSQYGPIA

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family

Homologues:

Organism=Escherichia coli, GI1789807, Length=510, Percent_Identity=54.7058823529412, Blast_Score=573, Evalue=1e-164,
Organism=Saccharomyces cerevisiae, GI6322950, Length=493, Percent_Identity=49.0872210953347, Blast_Score=469, Evalue=1e-133,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): PCKA_LEPBA (B0SFJ3)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001963939.1
- ProteinModelPortal:   B0SFJ3
- SMR:   B0SFJ3
- GeneID:   6388509
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_2886
- HOGENOM:   HBG293387
- OMA:   MFIRPTE
- ProtClustDB:   PRK09344
- BioCyc:   LBIF355278:LBF_2886-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- HAMAP:   MF_00453
- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994
- Gene3D:   G3DSA:3.90.228.20
- Gene3D:   G3DSA:3.40.449.10
- PIRSF:   PIRSF006294
- TIGRFAMs:   TIGR00224

Pfam domain/function: PF01293 PEPCK_ATP; SSF68923 PEP_carboxykinase_N

EC number: =4.1.1.49

Molecular weight: Translated: 59349; Mature: 59217

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure: 

>Translated Secondary Structure
MSVSTNLKGLAELGLKPSEVFHNLSYEEIYQHELNNKEGVTSDNGTMMVDTGIFTGRSPK
CCCCCCCHHHHHHCCCHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEEEECEECCCCCC
DKYFVDEPSSQNNIWWGPVNTKVSEAIFNELYAEVTKFLDNKKLYVFDGHAGTNDDTRIS
CCEEECCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEE
LRVVTERAWQHHFCTNMFLRPTKEELAKLDPEFTIINASGYKNPKYKEHGLNSEVFVIFH
EEEEEHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEEECCCCCCCCHHHCCCCCCEEEEEE
LAKKICIIGGTEYGGEMKKGIFSVMNYYLPLKNVLTMHCSANVGKDGDSALFFGLSGTGK
EHHHEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHEEEECCCCCCCCCCEEEEEECCCCC
TTLSTDPNRKLIGDDEHGWDDNGIFNIEGGCYAKTINLDPKTEPEIYAAIRRDALLENVV
EEECCCCCCCCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
YDATTKKVDYSSAAKTENTRVSYPIFHIDNIQPGSKAGHPNTVIFLTYDAYGVLPAVSKL
HCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCEEEEEEECCHHHHHHHHHH
SIEQAMYHFLSGYTAKVAGTERGVKEPQATFSACFGQAFMTLHPTYYAKLLGEKMKKHQV
HHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
NAYLINTGLVGGKYGVGKRMNLPATRQIINEILNGNIEKSEFEKHPVFQVSFPKSVNGVD
CEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHCCCCCEEEECCCCCCCCC
AHILNPRNAWENKEDYDKTAADLAKQFVENYKKYLTGSKEFDYSQYGPIA
EEEECCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SVSTNLKGLAELGLKPSEVFHNLSYEEIYQHELNNKEGVTSDNGTMMVDTGIFTGRSPK
CCCCCCHHHHHHCCCHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEEEECEECCCCCC
DKYFVDEPSSQNNIWWGPVNTKVSEAIFNELYAEVTKFLDNKKLYVFDGHAGTNDDTRIS
CCEEECCCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCEEE
LRVVTERAWQHHFCTNMFLRPTKEELAKLDPEFTIINASGYKNPKYKEHGLNSEVFVIFH
EEEEEHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEEECCCCCCCCHHHCCCCCCEEEEEE
LAKKICIIGGTEYGGEMKKGIFSVMNYYLPLKNVLTMHCSANVGKDGDSALFFGLSGTGK
EHHHEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHEEEECCCCCCCCCCEEEEEECCCCC
TTLSTDPNRKLIGDDEHGWDDNGIFNIEGGCYAKTINLDPKTEPEIYAAIRRDALLENVV
EEECCCCCCCCCCCCCCCCCCCCEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
YDATTKKVDYSSAAKTENTRVSYPIFHIDNIQPGSKAGHPNTVIFLTYDAYGVLPAVSKL
HCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCCCEEEEEEECCHHHHHHHHHH
SIEQAMYHFLSGYTAKVAGTERGVKEPQATFSACFGQAFMTLHPTYYAKLLGEKMKKHQV
HHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCC
NAYLINTGLVGGKYGVGKRMNLPATRQIINEILNGNIEKSEFEKHPVFQVSFPKSVNGVD
CEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHCCCCCEEEECCCCCCCCC
AHILNPRNAWENKEDYDKTAADLAKQFVENYKKYLTGSKEFDYSQYGPIA
EEEECCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA