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The map label for this gene is norB [H]

Identifier: 183222302

GI number: 183222302

Start: 3058872

End: 3060221

Strand: Reverse

Name: norB [H]

Synonym: LEPBI_I2954

Alternate gene names: 183222302

Gene position: 3060221-3058872 (Counterclockwise)

Preceding gene: 183222303

Following gene: 183222301

Centisome position: 85.01

GC content: 41.41

Gene sequence:

>1350_bases
ATGAGATTCCAATCACAAAAGGTCGCATATTGGTTCTTTGCAACTTGTATGTTACTCTTATCGTTACAAATCGTTTATGG
TTTTATCATGGGTTTTGCTCGTATCGGAATGGATGGATTACACGATTACATTCCATTTAACACGGCACGTGCGACACATA
CCAATTTACTCGTCGTTTGGTTATTAACAGGATTTATGGGTGCTGCGTATTACATCATCCCGGAAGAATCTGATAGGGAA
TTATACAGTGTAAAACTTGCTTATATCCAACTCATTTCTTGGGTTGTTGTAGGTGTTGTTGCCATTGTTGGTTTCCATTT
TAATTGGTGGGAAGGAAGAAAGTTTTTGGAAATTCCAAGACCTCTTGATTACCTTGTAGTAGTCAACGTTCTAACCTTTC
TTTTTAACATTGCTATGACAATTTGGGAAGCCAAAAAAAGAAGTACAACACAACTTGTCCTCTTCTTTGGTCTGTTATGT
GCTGCTCTATTGTACCTCCCAGGTATGATTTACTTCGACAACCAAACTCTCGATTCCTACTTTCGTTGGTGGGTCGTTCA
CCTTTGGGTAGAAGGAGTTTGGGAACTCATCATGGGTGGTATCTTAGCATTTTTACTCATCAAACTCACGGGAGTGGATA
GAGAAGTCATTGAAAAATGGTTGTATGTTGTTGTTGGTCTCACTTTCCTTTCTGGAATTTTGGGAACGGGACACCATTAC
TATTGGATCGGAACACCGAAGTATTGGCTCATGGTGGGTGGGATTTTCTCCGCGTTAGAACCGCTTGCCTTCCTTGGAAT
GGCGATTTGGGCACTCAATATGTACCGAAAAAAAGGAAAAGACCATCCAAACAAAATTGCACTCTATTGGACACTTGGCA
GTGCCATGATGTCTTTTATTGGAGCGGGTTTTCTTGGTTTTGCTCATACTTGGCCTGCGGTCAACCAATGGACACATGGA
ACTCTTGTCACCGCGATGCACGGACACCTTGCCTTCTGGGGAGCCTACGCTATGTTAGTGTTAGCTGTGATTTCATATGC
CATGCCAAACATGACAGGAAGAAAATTGTTTACTGGAATGTCTGGATATTTAGCATTTTGGGCTTCGAACATTGGAATGT
TAGGGATGACAGGTGCACTCGCTGTGGCAGGGATCACACAGGTATATCTCGAACGTAAATTGGGAATGGACTTTCTTGTC
GTACAAAAAGAAATCATTTTCCATTTTTACGGAATGTTACTTGCGGCGACCCTCTTTACGATTGGCATCACATACTTTAT
TGTGGATTTCATTCGACATGGACTTCCATCTAACGAAGCACTTGGAAAAAATGTAGGTGATCTAGATTAA

Upstream 100 bases:

>100_bases
AGCAATCAAACCGGGAACAAATATGCCGAAGTTGCCGTTAAGTGATACAGAAAGAAAGGACATCGTCACTTATCTTTCTG
CATTAAAATAAGGAGAAACA

Downstream 100 bases:

>100_bases
CTTATGTTAACAACAAAAATTCCCTATTACGAACCAATCGGTAAGGAAGTGGAAATTTTTCAAATGGCGGCGGAGAATTC
TCTGCCGCTTTTGTTAAAAG

Product: nitric-oxide reductase subunit B

Products: NA

Alternate protein names: NOR large subunit; Nitric oxide reductase cytochrome b subunit [H]

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MRFQSQKVAYWFFATCMLLLSLQIVYGFIMGFARIGMDGLHDYIPFNTARATHTNLLVVWLLTGFMGAAYYIIPEESDRE
LYSVKLAYIQLISWVVVGVVAIVGFHFNWWEGRKFLEIPRPLDYLVVVNVLTFLFNIAMTIWEAKKRSTTQLVLFFGLLC
AALLYLPGMIYFDNQTLDSYFRWWVVHLWVEGVWELIMGGILAFLLIKLTGVDREVIEKWLYVVVGLTFLSGILGTGHHY
YWIGTPKYWLMVGGIFSALEPLAFLGMAIWALNMYRKKGKDHPNKIALYWTLGSAMMSFIGAGFLGFAHTWPAVNQWTHG
TLVTAMHGHLAFWGAYAMLVLAVISYAMPNMTGRKLFTGMSGYLAFWASNIGMLGMTGALAVAGITQVYLERKLGMDFLV
VQKEIIFHFYGMLLAATLFTIGITYFIVDFIRHGLPSNEALGKNVGDLD

Sequences:

>Translated_449_residues
MRFQSQKVAYWFFATCMLLLSLQIVYGFIMGFARIGMDGLHDYIPFNTARATHTNLLVVWLLTGFMGAAYYIIPEESDRE
LYSVKLAYIQLISWVVVGVVAIVGFHFNWWEGRKFLEIPRPLDYLVVVNVLTFLFNIAMTIWEAKKRSTTQLVLFFGLLC
AALLYLPGMIYFDNQTLDSYFRWWVVHLWVEGVWELIMGGILAFLLIKLTGVDREVIEKWLYVVVGLTFLSGILGTGHHY
YWIGTPKYWLMVGGIFSALEPLAFLGMAIWALNMYRKKGKDHPNKIALYWTLGSAMMSFIGAGFLGFAHTWPAVNQWTHG
TLVTAMHGHLAFWGAYAMLVLAVISYAMPNMTGRKLFTGMSGYLAFWASNIGMLGMTGALAVAGITQVYLERKLGMDFLV
VQKEIIFHFYGMLLAATLFTIGITYFIVDFIRHGLPSNEALGKNVGDLD
>Mature_449_residues
MRFQSQKVAYWFFATCMLLLSLQIVYGFIMGFARIGMDGLHDYIPFNTARATHTNLLVVWLLTGFMGAAYYIIPEESDRE
LYSVKLAYIQLISWVVVGVVAIVGFHFNWWEGRKFLEIPRPLDYLVVVNVLTFLFNIAMTIWEAKKRSTTQLVLFFGLLC
AALLYLPGMIYFDNQTLDSYFRWWVVHLWVEGVWELIMGGILAFLLIKLTGVDREVIEKWLYVVVGLTFLSGILGTGHHY
YWIGTPKYWLMVGGIFSALEPLAFLGMAIWALNMYRKKGKDHPNKIALYWTLGSAMMSFIGAGFLGFAHTWPAVNQWTHG
TLVTAMHGHLAFWGAYAMLVLAVISYAMPNMTGRKLFTGMSGYLAFWASNIGMLGMTGALAVAGITQVYLERKLGMDFLV
VQKEIIFHFYGMLLAATLFTIGITYFIVDFIRHGLPSNEALGKNVGDLD

Specific function: Component of the anaerobic respiratory chain that transforms nitrate to dinitrogen (denitrification). NorB is the catalytic subunit of the enzyme complex. Shows proton pump activity across the membrane in denitrifying bacterial cells. The mononitrogen red

COG id: COG3256

COG function: function code P; Nitric oxide reductase large subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the heme-copper respiratory oxidase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000883 [H]

Pfam domain/function: PF00115 COX1 [H]

EC number: =1.7.99.7 [H]

Molecular weight: Translated: 50924; Mature: 50924

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: PS50855 COX1 ; PS00077 COX1_CUB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.9 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFQSQKVAYWFFATCMLLLSLQIVYGFIMGFARIGMDGLHDYIPFNTARATHTNLLVVW
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCHHHHHHHH
LLTGFMGAAYYIIPEESDRELYSVKLAYIQLISWVVVGVVAIVGFHFNWWEGRKFLEIPR
HHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCEEEECCC
PLDYLVVVNVLTFLFNIAMTIWEAKKRSTTQLVLFFGLLCAALLYLPGMIYFDNQTLDSY
CHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHH
FRWWVVHLWVEGVWELIMGGILAFLLIKLTGVDREVIEKWLYVVVGLTFLSGILGTGHHY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
YWIGTPKYWLMVGGIFSALEPLAFLGMAIWALNMYRKKGKDHPNKIALYWTLGSAMMSFI
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHH
GAGFLGFAHTWPAVNQWTHGTLVTAMHGHLAFWGAYAMLVLAVISYAMPNMTGRKLFTGM
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCH
SGYLAFWASNIGMLGMTGALAVAGITQVYLERKLGMDFLVVQKEIIFHFYGMLLAATLFT
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
IGITYFIVDFIRHGLPSNEALGKNVGDLD
HHHHHHHHHHHHCCCCCCCHHCCCCCCCC
>Mature Secondary Structure
MRFQSQKVAYWFFATCMLLLSLQIVYGFIMGFARIGMDGLHDYIPFNTARATHTNLLVVW
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCCCCCHHHHHHHH
LLTGFMGAAYYIIPEESDRELYSVKLAYIQLISWVVVGVVAIVGFHFNWWEGRKFLEIPR
HHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCEEEECCC
PLDYLVVVNVLTFLFNIAMTIWEAKKRSTTQLVLFFGLLCAALLYLPGMIYFDNQTLDSY
CHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCEEEECCCHHHHH
FRWWVVHLWVEGVWELIMGGILAFLLIKLTGVDREVIEKWLYVVVGLTFLSGILGTGHHY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
YWIGTPKYWLMVGGIFSALEPLAFLGMAIWALNMYRKKGKDHPNKIALYWTLGSAMMSFI
EEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEHHHHHHHHH
GAGFLGFAHTWPAVNQWTHGTLVTAMHGHLAFWGAYAMLVLAVISYAMPNMTGRKLFTGM
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCH
SGYLAFWASNIGMLGMTGALAVAGITQVYLERKLGMDFLVVQKEIIFHFYGMLLAATLFT
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
IGITYFIVDFIRHGLPSNEALGKNVGDLD
HHHHHHHHHHHHCCCCCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7508388; 2542222; 9521721 [H]