Click here to switch to the map view.

The map label for this gene is fadH [C]

Identifier: 183222224

GI number: 183222224

Start: 2967517

End: 2968719

Strand: Reverse

Name: fadH [C]

Synonym: LEPBI_I2875

Alternate gene names: 183222224

Gene position: 2968719-2967517 (Counterclockwise)

Preceding gene: 183222225

Following gene: 183222216

Centisome position: 82.47

GC content: 42.81

Gene sequence:

>1203_bases
ATGCATTTGAACTCAAACTTATTTTCTCCAATTGTCCTTCCCAATGGAATCTCATTACCCAATCGTTTGGTGAAAGCCGC
GATGGAAGAAAACCTCTCCAATGAAAATTTAGAGCCAGATGTTTCCCTTTGGAATTTATATGATGCCTGGGCAAAAGGGG
GAGTGGGAACTCTCATCACTGGCAATGTGATGGTGGACCATAGAGCGATGACAGGTCCTGGAGGAGTTGTTTTAGAAGAA
GGATCTTCCCTCATAGGATTTAAAAGATGGGCTGAAACAGTAAAATCAAACGGTACCAAGATCATCATGCAGATCAACCA
CCCTGGAAGGCAAATTTTGGCGAAGTTAGGTGGCAATGTTTGGGCACCTTCCGCCATTTCAGTCGATATTGGAAACCTTT
CCAAACTGATGGGAAAACCAAAGGCTATGGAAGAATCCGAAATTCTTGAAACCATCCAAAGGTTTGTTACCACGGCAAAA
CTCGCGGAAAACTCTGGATTTGATGGGGTTGAAATCCATGCCGCCCATGGGTATTTGATCAGCCAATTTTTATCTCCACT
TGTAAACCAAAGGACGGACAAATGGGGAGGGAGTTTGGCAAACCGCTCCCGGTTTTTAATGGAGGTCGTTCAGTCTGTTC
GCAAATCTGTGAAAGCTCATTTCATTGTTTCTGTAAAAATGAATTCAAGTGATTTTCAAAAAGGTGGCTTTCAGTTTGAA
GATGCAATCCAAGTAATAAAGAGAATACAAACGTTAGGTGTTGATTTTATTGAACTCTCTGGTGGAAACTATGAAGCACC
AGCCATGCAAGGAGTATCGAGGGATGGGTCTACTTTGGCAAGAGAAGCATACTTTTTAGACTTCGCTCGGGAAGTAACAA
AAATGGCAACGGTTCCCATTATGGTAACAGGTGGAATTGCAAGAAAACAAATTGCTGAGACAGTTTTGGAATCTGGTGTA
TCACTTGTTGGTGTAGCCACAGCACTTGCCCTCCATCCCAATTTACCAAACGATTGGAAGGAAAATCGAAATCCGATTTC
GAAATTGCCACTTCCAAATTGGAAGTCCAAAACTCTTGTTGGCCTTGCAACCATGGCAATGGTTCGTTACCAATTGCAAC
GACTCGCCGAAAAAAAGCACCAAAGGTTACAATATATCCATGGTTTCGATTGCTTGCGGATCAATTGCGATTGGCACGCC
TAA

Upstream 100 bases:

>100_bases
TAAAGTTCATCTCCTTTTGATCGAATTCTTCTTCGTCACAGTGATAAGAATTCCCATTCTCTTTACAATCCAATCATTGG
GTTTTGTCTAACCATTACCG

Downstream 100 bases:

>100_bases
CGAAACGGTATAGGCGATGGATGGAAAAATCTGGAGTTTGAATCGAATTGGTTCGAAAATTGAAATGGATTGGAAAATAA
AAAGGTAGAGCCAATGCCAA

Product: putative NADH oxidoreductase

Products: trans-transtetradehydroacyl-CoA; NADPH [C]

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MHLNSNLFSPIVLPNGISLPNRLVKAAMEENLSNENLEPDVSLWNLYDAWAKGGVGTLITGNVMVDHRAMTGPGGVVLEE
GSSLIGFKRWAETVKSNGTKIIMQINHPGRQILAKLGGNVWAPSAISVDIGNLSKLMGKPKAMEESEILETIQRFVTTAK
LAENSGFDGVEIHAAHGYLISQFLSPLVNQRTDKWGGSLANRSRFLMEVVQSVRKSVKAHFIVSVKMNSSDFQKGGFQFE
DAIQVIKRIQTLGVDFIELSGGNYEAPAMQGVSRDGSTLAREAYFLDFAREVTKMATVPIMVTGGIARKQIAETVLESGV
SLVGVATALALHPNLPNDWKENRNPISKLPLPNWKSKTLVGLATMAMVRYQLQRLAEKKHQRLQYIHGFDCLRINCDWHA

Sequences:

>Translated_400_residues
MHLNSNLFSPIVLPNGISLPNRLVKAAMEENLSNENLEPDVSLWNLYDAWAKGGVGTLITGNVMVDHRAMTGPGGVVLEE
GSSLIGFKRWAETVKSNGTKIIMQINHPGRQILAKLGGNVWAPSAISVDIGNLSKLMGKPKAMEESEILETIQRFVTTAK
LAENSGFDGVEIHAAHGYLISQFLSPLVNQRTDKWGGSLANRSRFLMEVVQSVRKSVKAHFIVSVKMNSSDFQKGGFQFE
DAIQVIKRIQTLGVDFIELSGGNYEAPAMQGVSRDGSTLAREAYFLDFAREVTKMATVPIMVTGGIARKQIAETVLESGV
SLVGVATALALHPNLPNDWKENRNPISKLPLPNWKSKTLVGLATMAMVRYQLQRLAEKKHQRLQYIHGFDCLRINCDWHA
>Mature_400_residues
MHLNSNLFSPIVLPNGISLPNRLVKAAMEENLSNENLEPDVSLWNLYDAWAKGGVGTLITGNVMVDHRAMTGPGGVVLEE
GSSLIGFKRWAETVKSNGTKIIMQINHPGRQILAKLGGNVWAPSAISVDIGNLSKLMGKPKAMEESEILETIQRFVTTAK
LAENSGFDGVEIHAAHGYLISQFLSPLVNQRTDKWGGSLANRSRFLMEVVQSVRKSVKAHFIVSVKMNSSDFQKGGFQFE
DAIQVIKRIQTLGVDFIELSGGNYEAPAMQGVSRDGSTLAREAYFLDFAREVTKMATVPIMVTGGIARKQIAETVLESGV
SLVGVATALALHPNLPNDWKENRNPISKLPLPNWKSKTLVGLATMAMVRYQLQRLAEKKHQRLQYIHGFDCLRINCDWHA

Specific function: Reduces a range of alternative electron acceptors [H]

COG id: COG1902

COG function: function code C; NADH:flavin oxidoreductases, Old Yellow Enzyme family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the N-terminal section; belongs to the NADH:flavin oxidoreductase/NADH oxidase family [H]

Homologues:

Organism=Escherichia coli, GI1789463, Length=318, Percent_Identity=30.188679245283, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1787939, Length=274, Percent_Identity=28.4671532846715, Blast_Score=89, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17565138, Length=340, Percent_Identity=35.2941176470588, Blast_Score=212, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI17540738, Length=340, Percent_Identity=35, Blast_Score=212, Evalue=3e-55,
Organism=Caenorhabditis elegans, GI17559802, Length=340, Percent_Identity=35.5882352941176, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI72001454, Length=338, Percent_Identity=34.0236686390533, Blast_Score=198, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17564188, Length=355, Percent_Identity=32.9577464788732, Blast_Score=198, Evalue=4e-51,
Organism=Caenorhabditis elegans, GI17559804, Length=338, Percent_Identity=34.0236686390533, Blast_Score=194, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17566914, Length=351, Percent_Identity=36.4672364672365, Blast_Score=182, Evalue=2e-46,
Organism=Caenorhabditis elegans, GI17566916, Length=342, Percent_Identity=32.7485380116959, Blast_Score=149, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6321973, Length=76, Percent_Identity=46.0526315789474, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6325086, Length=76, Percent_Identity=42.1052631578947, Blast_Score=76, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR013027
- InterPro:   IPR016040
- InterPro:   IPR001155
- InterPro:   IPR001327
- InterPro:   IPR000103 [H]

Pfam domain/function: PF00724 Oxidored_FMN; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.3.1.34 [C]

Molecular weight: Translated: 44016; Mature: 44016

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLNSNLFSPIVLPNGISLPNRLVKAAMEENLSNENLEPDVSLWNLYDAWAKGGVGTLIT
CCCCCCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEE
GNVMVDHRAMTGPGGVVLEEGSSLIGFKRWAETVKSNGTKIIMQINHPGRQILAKLGGNV
CCEEEECCEECCCCCEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCC
WAPSAISVDIGNLSKLMGKPKAMEESEILETIQRFVTTAKLAENSGFDGVEIHAAHGYLI
CCCCEEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHH
SQFLSPLVNQRTDKWGGSLANRSRFLMEVVQSVRKSVKAHFIVSVKMNSSDFQKGGFQFE
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCHHCCCCCHH
DAIQVIKRIQTLGVDFIELSGGNYEAPAMQGVSRDGSTLAREAYFLDFAREVTKMATVPI
HHHHHHHHHHHCCCCEEEECCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCE
MVTGGIARKQIAETVLESGVSLVGVATALALHPNLPNDWKENRNPISKLPLPNWKSKTLV
EEECCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCHHHH
GLATMAMVRYQLQRLAEKKHQRLQYIHGFDCLRINCDWHA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
>Mature Secondary Structure
MHLNSNLFSPIVLPNGISLPNRLVKAAMEENLSNENLEPDVSLWNLYDAWAKGGVGTLIT
CCCCCCCCCCEECCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCCCCEEEE
GNVMVDHRAMTGPGGVVLEEGSSLIGFKRWAETVKSNGTKIIMQINHPGRQILAKLGGNV
CCEEEECCEECCCCCEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHHCCCC
WAPSAISVDIGNLSKLMGKPKAMEESEILETIQRFVTTAKLAENSGFDGVEIHAAHGYLI
CCCCEEEEEHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHH
SQFLSPLVNQRTDKWGGSLANRSRFLMEVVQSVRKSVKAHFIVSVKMNSSDFQKGGFQFE
HHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCEEEEEEEEECCCCHHCCCCCHH
DAIQVIKRIQTLGVDFIELSGGNYEAPAMQGVSRDGSTLAREAYFLDFAREVTKMATVPI
HHHHHHHHHHHCCCCEEEECCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCE
MVTGGIARKQIAETVLESGVSLVGVATALALHPNLPNDWKENRNPISKLPLPNWKSKTLV
EEECCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCHHHHCCCCCCCCCCCCCCCHHHH
GLATMAMVRYQLQRLAEKKHQRLQYIHGFDCLRINCDWHA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD. [C]

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: transDidehydroacyl-CoA; NADP+ [C]

Specific reaction: transDidehydroacyl-CoA + NADP+ =trans-transtetradehydroacyl-CoA + NADPH [C]

General reaction: Redox reaction [C]

Inhibitor: Iodoacetic acid; N-Ethyl maleimide [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8357835 [H]