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The map label for this gene is rlmL [H]

Identifier: 183222196

GI number: 183222196

Start: 2937296

End: 2938279

Strand: Reverse

Name: rlmL [H]

Synonym: LEPBI_I2846

Alternate gene names: 183222196

Gene position: 2938279-2937296 (Counterclockwise)

Preceding gene: 183222203

Following gene: 183222193

Centisome position: 81.63

GC content: 34.65

Gene sequence:

>984_bases
ATGACCGAACGAGGATTAGGTGCCCTCACGATGTTTGAAAATCGCCTGCGTAAATTAAAAAAAGAACGCGAAAAATGGGC
GAAGCGAGAAAACATCAATTGTTACCGAATTTATTCAGAAGACATCCCTCAGATTCCATTTATTTTGGATCGGTATCCGA
ATGGTTTAGTATTATACGACAAAAGTTCCTTACGTTTCCAAGCTGAAACGGATCATGATGCACGATTGAAAGAAATTGTT
CGGATAGTTATGGAAGTATTTCAAATTCCTGAAAACAAACTCTTTTTAAAGCAGCGTAAAAAACAAAAAGGTTTAGACCA
ATATGAAAAATTTTCAATTGAAGGTAATTTTTTTTGGACTACGGAAGCGAACTTAGAATTTAGAATCAATTTGTCTGACT
ATTTGGATACAGGTTTATTTTTAGACCATCGCATCACCAGGTCTTGGTTAAAAGAAAAATCCAAAAATAAATCTGTTTTA
AATTTATTTTCCTACACAGGCGCTTTTTCCGTATATGCTGCGTCAGGTGGAGCTTCCAAAACAAAAAGTATTGATCTTTC
CAAAACTTATTGTGATTGGGCAGTTCAAAATTTGAGACGAAATGGTTTTGAAGGTTCGAATCATAAAATCATCAACGCAG
ACATTTTGAAGTGGATCGAAGAAGAGTCAAAAAATCCAAATCGAGAACGATATGATTTTATTTTTTTGGATCCACCTACA
TTTTCTAACAGTAAAAAAATGTACGAAGAATGGGAAGTCCAAACCAAACATCGAAACTTACTGTTGTTATTGCTAACGAA
ATTTTTATCGGATAAGGGTGAGATTTGGTTTTCAACGAATTTTCGGAAATTCAAAATGGAAGTAGAGGAGGAGGAATGGA
AAGAGAGAGGTTACCAGTGTTTGAATAGGACCAAAGAATCAATCCCTCCAGATTTTAGAGATGAGAAAATCCATCAATTA
TTCTGTATCATGCCTTTGGTTTAA

Upstream 100 bases:

>100_bases
GGTAAACGGAGCGTATTGGGTGGCGGGTGGATAACCCCACCCAGTCTCGATTAGGGCGGGGAAGTCTACCTAAAATACCC
GACCAATAAAGGAAAATCTC

Downstream 100 bases:

>100_bases
GGTTGCTTAATAGGAATTAGTTGACGAAAGGATCAATTCGCTCCGCTAAAATTCTTCGATTTTCGCCAGATTGTAATGAT
AAGATTCCTCTCATAATGGC

Product: hypothetical protein

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 327; Mature: 326

Protein sequence:

>327_residues
MTERGLGALTMFENRLRKLKKEREKWAKRENINCYRIYSEDIPQIPFILDRYPNGLVLYDKSSLRFQAETDHDARLKEIV
RIVMEVFQIPENKLFLKQRKKQKGLDQYEKFSIEGNFFWTTEANLEFRINLSDYLDTGLFLDHRITRSWLKEKSKNKSVL
NLFSYTGAFSVYAASGGASKTKSIDLSKTYCDWAVQNLRRNGFEGSNHKIINADILKWIEEESKNPNRERYDFIFLDPPT
FSNSKKMYEEWEVQTKHRNLLLLLLTKFLSDKGEIWFSTNFRKFKMEVEEEEWKERGYQCLNRTKESIPPDFRDEKIHQL
FCIMPLV

Sequences:

>Translated_327_residues
MTERGLGALTMFENRLRKLKKEREKWAKRENINCYRIYSEDIPQIPFILDRYPNGLVLYDKSSLRFQAETDHDARLKEIV
RIVMEVFQIPENKLFLKQRKKQKGLDQYEKFSIEGNFFWTTEANLEFRINLSDYLDTGLFLDHRITRSWLKEKSKNKSVL
NLFSYTGAFSVYAASGGASKTKSIDLSKTYCDWAVQNLRRNGFEGSNHKIINADILKWIEEESKNPNRERYDFIFLDPPT
FSNSKKMYEEWEVQTKHRNLLLLLLTKFLSDKGEIWFSTNFRKFKMEVEEEEWKERGYQCLNRTKESIPPDFRDEKIHQL
FCIMPLV
>Mature_326_residues
TERGLGALTMFENRLRKLKKEREKWAKRENINCYRIYSEDIPQIPFILDRYPNGLVLYDKSSLRFQAETDHDARLKEIVR
IVMEVFQIPENKLFLKQRKKQKGLDQYEKFSIEGNFFWTTEANLEFRINLSDYLDTGLFLDHRITRSWLKEKSKNKSVLN
LFSYTGAFSVYAASGGASKTKSIDLSKTYCDWAVQNLRRNGFEGSNHKIINADILKWIEEESKNPNRERYDFIFLDPPTF
SNSKKMYEEWEVQTKHRNLLLLLLTKFLSDKGEIWFSTNFRKFKMEVEEEEWKERGYQCLNRTKESIPPDFRDEKIHQLF
CIMPLV

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG1092

COG function: function code R; Predicted SAM-dependent methyltransferases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=313, Percent_Identity=42.4920127795527, Blast_Score=251, Evalue=5e-68,
Organism=Escherichia coli, GI145693111, Length=238, Percent_Identity=26.4705882352941, Blast_Score=91, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 39118; Mature: 38987

Theoretical pI: Translated: 9.31; Mature: 9.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTERGLGALTMFENRLRKLKKEREKWAKRENINCYRIYSEDIPQIPFILDRYPNGLVLYD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHCCCCCEEEEE
KSSLRFQAETDHDARLKEIVRIVMEVFQIPENKLFLKQRKKQKGLDQYEKFSIEGNFFWT
CCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHEEECCCEEEE
TEANLEFRINLSDYLDTGLFLDHRITRSWLKEKSKNKSVLNLFSYTGAFSVYAASGGASK
EECCEEEEEEHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCC
TKSIDLSKTYCDWAVQNLRRNGFEGSNHKIINADILKWIEEESKNPNRERYDFIFLDPPT
CCEECCHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHCCCCCCCCEEEEEECCCC
FSNSKKMYEEWEVQTKHRNLLLLLLTKFLSDKGEIWFSTNFRKFKMEVEEEEWKERGYQC
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEECCHHHHHHHHHHH
LNRTKESIPPDFRDEKIHQLFCIMPLV
HHHHHHHCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
TERGLGALTMFENRLRKLKKEREKWAKRENINCYRIYSEDIPQIPFILDRYPNGLVLYD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHCCCCCEEEEE
KSSLRFQAETDHDARLKEIVRIVMEVFQIPENKLFLKQRKKQKGLDQYEKFSIEGNFFWT
CCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHEEECCCEEEE
TEANLEFRINLSDYLDTGLFLDHRITRSWLKEKSKNKSVLNLFSYTGAFSVYAASGGASK
EECCEEEEEEHHHHHCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCEEEEEECCCCCC
TKSIDLSKTYCDWAVQNLRRNGFEGSNHKIINADILKWIEEESKNPNRERYDFIFLDPPT
CCEECCHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHCCCCCCCCEEEEEECCCC
FSNSKKMYEEWEVQTKHRNLLLLLLTKFLSDKGEIWFSTNFRKFKMEVEEEEWKERGYQC
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEECCHHHHHHHHHHH
LNRTKESIPPDFRDEKIHQLFCIMPLV
HHHHHHHCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]