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The map label for this gene is 183222110

Identifier: 183222110

GI number: 183222110

Start: 2844501

End: 2846621

Strand: Reverse

Name: 183222110

Synonym: LEPBI_I2752

Alternate gene names: NA

Gene position: 2846621-2844501 (Counterclockwise)

Preceding gene: 183222116

Following gene: 183222109

Centisome position: 79.08

GC content: 39.23

Gene sequence:

>2121_bases
TTGTGTTTGTTTCGATTGGAAACGGCAGGTTTCCAACACCTTTATGGCTTCAAAATAGTCGACAATTGTTCGGGATTGAC
AGTTTTGCCTGTCGATGGTAAAACGAGTAGGATGAAACTTCGGATTTTTTTGACACTCTTTCTTCTAATGACTCTTCCGA
CATTTGCGGATGAACCGAACGCGTTTTTGGCAGGGATGTCCAAAAAAGACATCACAGGTCCACCGATCGGTGTCATGTTT
TGGGGCTATGCAAGGGAGGACCAAACCGGTGTTGGGATCCAAACAAGGCAATACGCCAGAGCACTTGTCATTGAAGATCG
TCTTACCCACAAATTATTCGCTTATGTAACTGCTGAGGTAGGTGGAATTCCTTTTGAGATCCAAAGGGAGGTTGTGGCCA
AACTCCAAACGGAAGTGGATGCAAATTTCCATTTAGGAAATGTTTTGCTAAATGCTTCACATACTCATAGCGGACCTGCG
GGTCATTTTCATTATTCGGAAGTATCTTTTTATTCTACTCAGTTTTACAAAGACACCTATCAAGTATTACGTGATGGTAT
TGTGGAAGCAATCAAAGAGGCTTATCAAAAAAGAAAACCTGCCGATTTAAAAATTGGGAAAACAATTGTAAAGGATGCTG
GGGTGAATCGAAGTTTGTCTGCATATATGGCGAATCCAGAATCGGAAAGAAAATTATATGCTGATAATATTGATAAAGAG
ATGGTGCAACTCAATGTTTTTGTGGAAGGAAAATCAGTTGGATTTGTCAATTGGTATGGAGTCCATCCAACAAATATAAC
ATTTGATAATCGATTGATTTCATCAGATAATAAAGGAATTGCTTCGATACTGTCTGAAAAAGAAGCAGAGAAAAATGGAG
ACAAGGGTTTTGTTGCAATTTTTGCACAAGCCAATGAAGGTGATGTATCTCCAAATTTAAATTTAAATAATACAGGTCCT
GGTAAAGATATTTATGATAGTAGTTTCATTATCGGACAACGTCAATTTTTAGCAAGCCAAGAGATATTAAATGCAAAAGG
AAAATCATTAAAAGGTGGATTGAGTTTTTCACATCGATTCATTGATATGAGCCAACACCCAGTGAGAAGTGAATTCTCTG
GAACTGGCAAAACAGAATACACATGTCCTTCCGCGTATGGATATGCATTTGCTGCGGGGTCAACGGAAGAAGGTGGTGGG
CACTTTTTATTCCATGAAGGAATGACTGAAAAAAATCGAAGATTTTACATTGATTGGATTGCAAAGTTTATGTTACAGTC
CCCATCAGAAAAATTGAGAGAATGCCAAAATCCAAAGGCTGTTTTGTTTCCGATGGGTGAAACAAAACCAATTCCAAGTT
TGCCACAAATTCTACCGTATGGACTTGTGTTAGTTGGTGAATTATCCATAATGGTTCTGCCACACGAAGTCACGACCATG
TCTAGTCGGAGATTAAAAAAAGAAGTTAAGTCGGTTTTGAAAGACAAAATATCTGATATTGTTCTTTCTGGATTGACCAA
CGACTTTTCTGGTTACATCACAACTCCAGAAGAATACTCTACTCAGAATTATGAAGGTGGCCATACACTCCATGGACAAC
AAAGCCTAAATGCCCTTAGGCAGGAATTTTTTGGAATGGCAAGTGATCTCATCCAAGGTATTGAATTTACAAATGCTTCC
AAGTCGGAAATCACTCCATTAGACTTATCGGGAAAGGTGCATCCACTGAAACTTCCTGAATCAGATACAGTCACAACTGA
ACCCAAACAAGTGTTAAAGCCAAATGGAAATCTGTATCAAAAAGGAAACCAAGTGGTTTGTAAAGTCAGTGCAGCAAGTC
CAAATGTGGGATACCCTAATGTGAATTCCTATTTGTGGGTTGAAAAAAAGAATGGTTCCGGTTGGGAGAAGGTTCGATCA
GATTCTGACTTTGATACCAAATTCACTTACCAAAGCCCTGGATTTTTTGACAAAGGTTTAGGAAAGATGGAATTGGTTTG
GGAGACGAGTCCATCCATTCCCAATGGCGAATACCGTTTGGTTCACGAAGGGATTTACCTTTCTCCCACTGAGAAAAAAA
GTAATTACCGGGTGGAATGCCCTGTCTTCCAAGTCCAATAA

Upstream 100 bases:

>100_bases
TTTTTACTTTCACCAACTTCGAGTGATACCTAGTTTTCGTCCGTACTTTCTTTGCCTAGACCTAGTGTCTTAATGCTCTA
AAAAAGAACTCATCTGGATT

Downstream 100 bases:

>100_bases
AACGATTTAATGAGTTGGTCTGTTCTTTGATTTAGACACAGACCAGCTCCCATGAAACCAAGGGCACTCCCAAGTGGATC
CCTTTCTCATCATAAAAACA

Product: putative neutral/alkaline ceramidase

Products: NA

Alternate protein names: N-CDase; NCDase; Acylsphingosine deacylase; N-acylsphingosine amidohydrolase [H]

Number of amino acids: Translated: 706; Mature: 706

Protein sequence:

>706_residues
MCLFRLETAGFQHLYGFKIVDNCSGLTVLPVDGKTSRMKLRIFLTLFLLMTLPTFADEPNAFLAGMSKKDITGPPIGVMF
WGYAREDQTGVGIQTRQYARALVIEDRLTHKLFAYVTAEVGGIPFEIQREVVAKLQTEVDANFHLGNVLLNASHTHSGPA
GHFHYSEVSFYSTQFYKDTYQVLRDGIVEAIKEAYQKRKPADLKIGKTIVKDAGVNRSLSAYMANPESERKLYADNIDKE
MVQLNVFVEGKSVGFVNWYGVHPTNITFDNRLISSDNKGIASILSEKEAEKNGDKGFVAIFAQANEGDVSPNLNLNNTGP
GKDIYDSSFIIGQRQFLASQEILNAKGKSLKGGLSFSHRFIDMSQHPVRSEFSGTGKTEYTCPSAYGYAFAAGSTEEGGG
HFLFHEGMTEKNRRFYIDWIAKFMLQSPSEKLRECQNPKAVLFPMGETKPIPSLPQILPYGLVLVGELSIMVLPHEVTTM
SSRRLKKEVKSVLKDKISDIVLSGLTNDFSGYITTPEEYSTQNYEGGHTLHGQQSLNALRQEFFGMASDLIQGIEFTNAS
KSEITPLDLSGKVHPLKLPESDTVTTEPKQVLKPNGNLYQKGNQVVCKVSAASPNVGYPNVNSYLWVEKKNGSGWEKVRS
DSDFDTKFTYQSPGFFDKGLGKMELVWETSPSIPNGEYRLVHEGIYLSPTEKKSNYRVECPVFQVQ

Sequences:

>Translated_706_residues
MCLFRLETAGFQHLYGFKIVDNCSGLTVLPVDGKTSRMKLRIFLTLFLLMTLPTFADEPNAFLAGMSKKDITGPPIGVMF
WGYAREDQTGVGIQTRQYARALVIEDRLTHKLFAYVTAEVGGIPFEIQREVVAKLQTEVDANFHLGNVLLNASHTHSGPA
GHFHYSEVSFYSTQFYKDTYQVLRDGIVEAIKEAYQKRKPADLKIGKTIVKDAGVNRSLSAYMANPESERKLYADNIDKE
MVQLNVFVEGKSVGFVNWYGVHPTNITFDNRLISSDNKGIASILSEKEAEKNGDKGFVAIFAQANEGDVSPNLNLNNTGP
GKDIYDSSFIIGQRQFLASQEILNAKGKSLKGGLSFSHRFIDMSQHPVRSEFSGTGKTEYTCPSAYGYAFAAGSTEEGGG
HFLFHEGMTEKNRRFYIDWIAKFMLQSPSEKLRECQNPKAVLFPMGETKPIPSLPQILPYGLVLVGELSIMVLPHEVTTM
SSRRLKKEVKSVLKDKISDIVLSGLTNDFSGYITTPEEYSTQNYEGGHTLHGQQSLNALRQEFFGMASDLIQGIEFTNAS
KSEITPLDLSGKVHPLKLPESDTVTTEPKQVLKPNGNLYQKGNQVVCKVSAASPNVGYPNVNSYLWVEKKNGSGWEKVRS
DSDFDTKFTYQSPGFFDKGLGKMELVWETSPSIPNGEYRLVHEGIYLSPTEKKSNYRVECPVFQVQ
>Mature_706_residues
MCLFRLETAGFQHLYGFKIVDNCSGLTVLPVDGKTSRMKLRIFLTLFLLMTLPTFADEPNAFLAGMSKKDITGPPIGVMF
WGYAREDQTGVGIQTRQYARALVIEDRLTHKLFAYVTAEVGGIPFEIQREVVAKLQTEVDANFHLGNVLLNASHTHSGPA
GHFHYSEVSFYSTQFYKDTYQVLRDGIVEAIKEAYQKRKPADLKIGKTIVKDAGVNRSLSAYMANPESERKLYADNIDKE
MVQLNVFVEGKSVGFVNWYGVHPTNITFDNRLISSDNKGIASILSEKEAEKNGDKGFVAIFAQANEGDVSPNLNLNNTGP
GKDIYDSSFIIGQRQFLASQEILNAKGKSLKGGLSFSHRFIDMSQHPVRSEFSGTGKTEYTCPSAYGYAFAAGSTEEGGG
HFLFHEGMTEKNRRFYIDWIAKFMLQSPSEKLRECQNPKAVLFPMGETKPIPSLPQILPYGLVLVGELSIMVLPHEVTTM
SSRRLKKEVKSVLKDKISDIVLSGLTNDFSGYITTPEEYSTQNYEGGHTLHGQQSLNALRQEFFGMASDLIQGIEFTNAS
KSEITPLDLSGKVHPLKLPESDTVTTEPKQVLKPNGNLYQKGNQVVCKVSAASPNVGYPNVNSYLWVEKKNGSGWEKVRS
DSDFDTKFTYQSPGFFDKGLGKMELVWETSPSIPNGEYRLVHEGIYLSPTEKKSNYRVECPVFQVQ

Specific function: Hydrolyzes the ceramides into sphingosine and free fatty acid at an optimal pH of 8-9 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the neutral ceramidase family [H]

Homologues:

Organism=Homo sapiens, GI221218981, Length=662, Percent_Identity=32.9305135951662, Blast_Score=317, Evalue=3e-86,
Organism=Homo sapiens, GI221307475, Length=662, Percent_Identity=32.1752265861027, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24651405, Length=693, Percent_Identity=30.4473304473304, Blast_Score=311, Evalue=1e-84,
Organism=Drosophila melanogaster, GI24651403, Length=693, Percent_Identity=30.4473304473304, Blast_Score=311, Evalue=1e-84,
Organism=Drosophila melanogaster, GI24651401, Length=693, Percent_Identity=30.4473304473304, Blast_Score=311, Evalue=1e-84,
Organism=Drosophila melanogaster, GI24651397, Length=693, Percent_Identity=30.4473304473304, Blast_Score=311, Evalue=1e-84,
Organism=Drosophila melanogaster, GI24651399, Length=693, Percent_Identity=30.4473304473304, Blast_Score=311, Evalue=1e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006823 [H]

Pfam domain/function: NA

EC number: =3.5.1.23 [H]

Molecular weight: Translated: 78658; Mature: 78658

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCLFRLETAGFQHLYGFKIVDNCSGLTVLPVDGKTSRMKLRIFLTLFLLMTLPTFADEPN
CEEEEECCCCCCHHCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
AFLAGMSKKDITGPPIGVMFWGYAREDQTGVGIQTRQYARALVIEDRLTHKLFAYVTAEV
HHEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GGIPFEIQREVVAKLQTEVDANFHLGNVLLNASHTHSGPAGHFHYSEVSFYSTQFYKDTY
CCCCHHHHHHHHHHHHHHCCCCEEECEEEEECCCCCCCCCCCEEECEEEEHHHHHHHHHH
QVLRDGIVEAIKEAYQKRKPADLKIGKTIVKDAGVNRSLSAYMANPESERKLYADNIDKE
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEECCCCHHCCCHHCCCCCE
MVQLNVFVEGKSVGFVNWYGVHPTNITFDNRLISSDNKGIASILSEKEAEKNGDKGFVAI
EEEEEEEEECCEEEEEEEECCCCCCEEECCEEECCCCCHHHHHHHHHHHHCCCCCEEEEE
FAQANEGDVSPNLNLNNTGPGKDIYDSSFIIGQRQFLASQEILNAKGKSLKGGLSFSHRF
EEECCCCCCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHCCCCCCCCCCCCCEEE
IDMSQHPVRSEFSGTGKTEYTCPSAYGYAFAAGSTEEGGGHFLFHEGMTEKNRRFYIDWI
ECCCCCCCCHHCCCCCCCCEECCCCCCEEEECCCCCCCCCEEEEECCCCCCCCEEEHHHH
AKFMLQSPSEKLRECQNPKAVLFPMGETKPIPSLPQILPYGLVLVGELSIMVLPHEVTTM
HHHHHCCCHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHEEEEECEEEEEECCHHHHH
SSRRLKKEVKSVLKDKISDIVLSGLTNDFSGYITTPEEYSTQNYEGGHTLHGQQSLNALR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHCCCCCCCCCEECCHHHHHHHH
QEFFGMASDLIQGIEFTNASKSEITPLDLSGKVHPLKLPESDTVTTEPKQVLKPNGNLYQ
HHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCEEEEECCCCCCCCCCCHHHHCCCCCEEE
KGNQVVCKVSAASPNVGYPNVNSYLWVEKKNGSGWEKVRSDSDFDTKFTYQSPGFFDKGL
CCCEEEEEEECCCCCCCCCCCCCEEEEECCCCCCHHHHCCCCCCCCEEEECCCCCHHCCC
GKMELVWETSPSIPNGEYRLVHEGIYLSPTEKKSNYRVECPVFQVQ
CCEEEEEECCCCCCCCCEEEEECCEEECCCCCCCCEEEECCEEEEC
>Mature Secondary Structure
MCLFRLETAGFQHLYGFKIVDNCSGLTVLPVDGKTSRMKLRIFLTLFLLMTLPTFADEPN
CEEEEECCCCCCHHCCEEEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
AFLAGMSKKDITGPPIGVMFWGYAREDQTGVGIQTRQYARALVIEDRLTHKLFAYVTAEV
HHEECCCCCCCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHC
GGIPFEIQREVVAKLQTEVDANFHLGNVLLNASHTHSGPAGHFHYSEVSFYSTQFYKDTY
CCCCHHHHHHHHHHHHHHCCCCEEECEEEEECCCCCCCCCCCEEECEEEEHHHHHHHHHH
QVLRDGIVEAIKEAYQKRKPADLKIGKTIVKDAGVNRSLSAYMANPESERKLYADNIDKE
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCCCCEEECCCCHHCCCHHCCCCCE
MVQLNVFVEGKSVGFVNWYGVHPTNITFDNRLISSDNKGIASILSEKEAEKNGDKGFVAI
EEEEEEEEECCEEEEEEEECCCCCCEEECCEEECCCCCHHHHHHHHHHHHCCCCCEEEEE
FAQANEGDVSPNLNLNNTGPGKDIYDSSFIIGQRQFLASQEILNAKGKSLKGGLSFSHRF
EEECCCCCCCCCCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHCCCCCCCCCCCCCEEE
IDMSQHPVRSEFSGTGKTEYTCPSAYGYAFAAGSTEEGGGHFLFHEGMTEKNRRFYIDWI
ECCCCCCCCHHCCCCCCCCEECCCCCCEEEECCCCCCCCCEEEEECCCCCCCCEEEHHHH
AKFMLQSPSEKLRECQNPKAVLFPMGETKPIPSLPQILPYGLVLVGELSIMVLPHEVTTM
HHHHHCCCHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHHHEEEEECEEEEEECCHHHHH
SSRRLKKEVKSVLKDKISDIVLSGLTNDFSGYITTPEEYSTQNYEGGHTLHGQQSLNALR
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHCCCCCCCCCEECCHHHHHHHH
QEFFGMASDLIQGIEFTNASKSEITPLDLSGKVHPLKLPESDTVTTEPKQVLKPNGNLYQ
HHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCEEEEECCCCCCCCCCCHHHHCCCCCEEE
KGNQVVCKVSAASPNVGYPNVNSYLWVEKKNGSGWEKVRSDSDFDTKFTYQSPGFFDKGL
CCCEEEEEEECCCCCCCCCCCCCEEEEECCCCCCHHHHCCCCCCCCEEEECCCCCHHCCC
GKMELVWETSPSIPNGEYRLVHEGIYLSPTEKKSNYRVECPVFQVQ
CCEEEEEECCCCCCCCCEEEEECCEEECCCCCCCCEEEECCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10593963; 10984043 [H]