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The map label for this gene is purT [H]

Identifier: 183222093

GI number: 183222093

Start: 2827100

End: 2828275

Strand: Reverse

Name: purT [H]

Synonym: LEPBI_I2735

Alternate gene names: 183222093

Gene position: 2828275-2827100 (Counterclockwise)

Preceding gene: 183222094

Following gene: 183222092

Centisome position: 78.57

GC content: 44.9

Gene sequence:

>1176_bases
ATGATCGGAACACCTTTTACCAAACATGCGACAAAACTCCTCCTCTTAGGTTCTGGAGAATTAGGAAAAGAGGTAGCCAT
CGAAGCCAATCGATTGGGTGTTCATGTCATCGCCGTGGATCGTTATCCCAACGCACCAGCTATGCTTGTGGCACAAGAAT
CCCGGGTCATCAATATGCTCGATCCAAAAGAACTAGAGGCCACCATACGCGAGTTAAAGCCTGATTTTGTTGTCCCGGAA
ATTGAGGCCATCCATACCGAAACTCTTGTTACGTTAGAAAAAGATGGATTTAAGATCATACCGAGTGCCAAAGCAGTGAA
CCTCACCATGAACCGTGAAGGCATTCGTAATTTCGTTGCAAACGAACTGGGTTTAATAACCTCCCAATTCCTTTTTGCCG
ACACCATCGAAGATTTTACAAACGCAGTCCATACTATTGGTTTTCCTTGTGTCGTGAAACCCATTATGAGTTCTTCTGGG
AAAGGACAAAGTTTAGTCAAAACAGAAGCCGATATAATAAAGGCATGGGAGTATGGCCAAACCGGAGGTCGGACTGGCAA
AGGGAAGATGATCATTGAAGAGTTCATCCCTTTTGATTTTGAAATCACACTTCTTACGATCCGCCATATCGATGGAACCA
GTTTTTTACCTCCCATTGGGCATAGACAAGTGAACGGGGATTATGTGGAGTCTTGGATGCCGCAACCCATGTCTGACCTA
GCCTTAACATCCGCCAAAAACATTGCAGAAACTGTGACAACAGGCCTTGGTGGGTTTGGGATCTTTGGTGTGGAATTATT
TGTGAAAGGGGACGAAGTGTACTTTAGTGAAGTCTCACCTAGGCCACATGACACAGGACTTGTGACCCTTATCTCCCAAA
ATGTTTCTGAGTTTTCCCTCCATGTGCGTGCCTTACTTGGGCTTCCCATCCCCGAACTTATCTTCCAAACACCTGCGGCA
AGTTCTGCCATTTTACTCGAAGGGAATACCAAATCGCCTGAGTATGTAGGGCTAAAAGAAGCACTGGCAATCAAAGGTGT
GGACATTCGCATTTTTGGGAAACCAGAAGTGGAAGGAAAACGCCGAATGGGTGTTAGTTTGGCACTTGGAAACACGATTG
AAGAAGCAAAAGAAAAAGCCAATCGTGGTCGAGATCACATCCAATTAAAAACTTAA

Upstream 100 bases:

>100_bases
AATGGGTGCCAATATTTACCAACGAGACAATTTAGGAAAACGATATTTGATGTAAGGATTCTTTCTTGTCGTATATGGCG
GTGTCTCCATCTTTGCGAAT

Downstream 100 bases:

>100_bases
ATTTTCCATTTTGACGGTGTGATATCAATTTCGTTTCTAGGGAGTTATGTGCATTCGTAAAAAGCAAACTATACCGATAA
AACAATGAACCTTGACTTTG

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2 [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MIGTPFTKHATKLLLLGSGELGKEVAIEANRLGVHVIAVDRYPNAPAMLVAQESRVINMLDPKELEATIRELKPDFVVPE
IEAIHTETLVTLEKDGFKIIPSAKAVNLTMNREGIRNFVANELGLITSQFLFADTIEDFTNAVHTIGFPCVVKPIMSSSG
KGQSLVKTEADIIKAWEYGQTGGRTGKGKMIIEEFIPFDFEITLLTIRHIDGTSFLPPIGHRQVNGDYVESWMPQPMSDL
ALTSAKNIAETVTTGLGGFGIFGVELFVKGDEVYFSEVSPRPHDTGLVTLISQNVSEFSLHVRALLGLPIPELIFQTPAA
SSAILLEGNTKSPEYVGLKEALAIKGVDIRIFGKPEVEGKRRMGVSLALGNTIEEAKEKANRGRDHIQLKT

Sequences:

>Translated_391_residues
MIGTPFTKHATKLLLLGSGELGKEVAIEANRLGVHVIAVDRYPNAPAMLVAQESRVINMLDPKELEATIRELKPDFVVPE
IEAIHTETLVTLEKDGFKIIPSAKAVNLTMNREGIRNFVANELGLITSQFLFADTIEDFTNAVHTIGFPCVVKPIMSSSG
KGQSLVKTEADIIKAWEYGQTGGRTGKGKMIIEEFIPFDFEITLLTIRHIDGTSFLPPIGHRQVNGDYVESWMPQPMSDL
ALTSAKNIAETVTTGLGGFGIFGVELFVKGDEVYFSEVSPRPHDTGLVTLISQNVSEFSLHVRALLGLPIPELIFQTPAA
SSAILLEGNTKSPEYVGLKEALAIKGVDIRIFGKPEVEGKRRMGVSLALGNTIEEAKEKANRGRDHIQLKT
>Mature_391_residues
MIGTPFTKHATKLLLLGSGELGKEVAIEANRLGVHVIAVDRYPNAPAMLVAQESRVINMLDPKELEATIRELKPDFVVPE
IEAIHTETLVTLEKDGFKIIPSAKAVNLTMNREGIRNFVANELGLITSQFLFADTIEDFTNAVHTIGFPCVVKPIMSSSG
KGQSLVKTEADIIKAWEYGQTGGRTGKGKMIIEEFIPFDFEITLLTIRHIDGTSFLPPIGHRQVNGDYVESWMPQPMSDL
ALTSAKNIAETVTTGLGGFGIFGVELFVKGDEVYFSEVSPRPHDTGLVTLISQNVSEFSLHVRALLGLPIPELIFQTPAA
SSAILLEGNTKSPEYVGLKEALAIKGVDIRIFGKPEVEGKRRMGVSLALGNTIEEAKEKANRGRDHIQLKT

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP [H]

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1788155, Length=390, Percent_Identity=53.8461538461538, Blast_Score=437, Evalue=1e-124,
Organism=Escherichia coli, GI1786733, Length=313, Percent_Identity=24.6006389776358, Blast_Score=68, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6324702, Length=394, Percent_Identity=27.4111675126904, Blast_Score=123, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42672; Mature: 42672

Theoretical pI: Translated: 5.67; Mature: 5.67

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGTPFTKHATKLLLLGSGELGKEVAIEANRLGVHVIAVDRYPNAPAMLVAQESRVINML
CCCCCCCCCCEEEEEEECCCCCCEEEEEECCCEEEEEEEECCCCCCEEEEEECCCEEECC
DPKELEATIRELKPDFVVPEIEAIHTETLVTLEKDGFKIIPSAKAVNLTMNREGIRNFVA
CHHHHHHHHHHCCCCCCCCCHHHHCCEEEEEEECCCCEEECCCEEEEEEECHHHHHHHHH
NELGLITSQFLFADTIEDFTNAVHTIGFPCVVKPIMSSSGKGQSLVKTEADIIKAWEYGQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC
TGGRTGKGKMIIEEFIPFDFEITLLTIRHIDGTSFLPPIGHRQVNGDYVESWMPQPMSDL
CCCCCCCCCEEHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCHHHH
ALTSAKNIAETVTTGLGGFGIFGVELFVKGDEVYFSEVSPRPHDTGLVTLISQNVSEFSL
HHHHHHHHHHHHHHCCCCCEEEEEEEEEECCEEEEECCCCCCCCCCEEEEECCCHHHHHH
HVRALLGLPIPELIFQTPAASSAILLEGNTKSPEYVGLKEALAIKGVDIRIFGKPEVEGK
HHHHHHCCCHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHEECCEEEEEECCCCCCCC
RRMGVSLALGNTIEEAKEKANRGRDHIQLKT
HHCCEEEEECCHHHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure
MIGTPFTKHATKLLLLGSGELGKEVAIEANRLGVHVIAVDRYPNAPAMLVAQESRVINML
CCCCCCCCCCEEEEEEECCCCCCEEEEEECCCEEEEEEEECCCCCCEEEEEECCCEEECC
DPKELEATIRELKPDFVVPEIEAIHTETLVTLEKDGFKIIPSAKAVNLTMNREGIRNFVA
CHHHHHHHHHHCCCCCCCCCHHHHCCEEEEEEECCCCEEECCCEEEEEEECHHHHHHHHH
NELGLITSQFLFADTIEDFTNAVHTIGFPCVVKPIMSSSGKGQSLVKTEADIIKAWEYGQ
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCC
TGGRTGKGKMIIEEFIPFDFEITLLTIRHIDGTSFLPPIGHRQVNGDYVESWMPQPMSDL
CCCCCCCCCEEHHHHCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCHHHH
ALTSAKNIAETVTTGLGGFGIFGVELFVKGDEVYFSEVSPRPHDTGLVTLISQNVSEFSL
HHHHHHHHHHHHHHCCCCCEEEEEEEEEECCEEEEECCCCCCCCCCEEEEECCCHHHHHH
HVRALLGLPIPELIFQTPAASSAILLEGNTKSPEYVGLKEALAIKGVDIRIFGKPEVEGK
HHHHHHCCCHHHHHHCCCCCCCEEEEECCCCCCCCCCHHHHHEECCEEEEEECCCCCCCC
RRMGVSLALGNTIEEAKEKANRGRDHIQLKT
HHCCEEEEECCHHHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA