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The map label for this gene is thrS [H]

Identifier: 183221772

GI number: 183221772

Start: 2470440

End: 2472356

Strand: Reverse

Name: thrS [H]

Synonym: LEPBI_I2402

Alternate gene names: 183221772

Gene position: 2472356-2470440 (Counterclockwise)

Preceding gene: 183221773

Following gene: 183221771

Centisome position: 68.68

GC content: 42.04

Gene sequence:

>1917_bases
ATGGCAGCACTTACAATTACATTACCCGATGGAAGTTCCAAAGAACTCGAATCAGGAAAGTCATTTTCTGATTTTGTCCA
AAACCAACTGCCTTTCTTAAAAGAGAAAGCTCTTGCCGTTGTTTTGTCCGATGGACGAACCGTTGATTTATCTTTTGTTC
CAACCGAAAACACGACTGTCAAATTTCTCACCTTCGATGACAAAGAGGGAAAGGAAGTATTCCACCATTCATCTGCCCAC
TTACTCGGAATGGCCGTCCAAAGGTTATGGCCCGAAGCAAGGCTCACTGTAGGCCCTGTCATTGAAAATGGACCTGGATT
TTTCTTTTATGATATCGATTTTGGAGACTTGGTCATCACAGTGGATGACCTTCCCAAAATTGAAGCCGAAATGGCAAAAA
TTGTGAAAGAAGACCTAACCGTAAAACGGTGGGAACTTTCCAAAGAAGAAGCGATAGAAAAATTCAAAAAAGAAAACGAA
CCTTATAAAGTAGAACTCATCCAAGGTTTTGATGCCGCCTCAGTTTCGTTATACGGACAAGGGGAGTGGTATGACCTTTG
CCGTGGACCACATGTGGCGCGAACAGGCCAACTCAAAGCCTTCAAACTCACAGCAATTTCTGGTGCCTATTGGAAAGGCG
ATTCCAAAAACAAACAACTCACTCGTATTTATGGAGTATCCTTCCCCACCAAAAAACTGTTAGATGAATATATCTTTCTC
ATCGAAGAGGCGAAAAAAAGGGACCATCGAAAACTCGGAAAAGAATTGGATTTGTTTAGTTTCCAAGATGAGGCACCTGG
TTTTCCGTTTTGGCATCCAAAAGGTACAGTGTTATGGAACACCCTAGCATCGTATATCCGAGAAGAATGTTTTCGTCGTG
GGTATCAGGAAATCAAAACTCCCGCCATTTTAAATTCCAGCCTTTGGAAAAAGTCCGGGCACTGGGATAATTTTAAAGAA
AACATGTATTTCACTGACATCGATGAAAGTGAGTTTGCCGTCAAACCCATGAACTGTCCTGGATGTTGTTTGATTTACAA
ATACCATATGCACTCTTACAGAGAACTACCCCTTCGGTTTATGGAACTCGGGAATGTGCATCGTCACGAAATGTCTGGGG
TTTTGCATGGACTCTTCCGTGTTCGTGCCTTTACCCAAGACGATGCCCACATCTATGCTCCTCTTGATAAAGTAGAATCG
GAAGTAGAAGACATCATTGACTTTACATTTGATGTGTACAAAAAGTTTGGATTCACTGAATTCAAAACCTTCATCGCGAC
AAGACCTGAAAAGTCACAAGGTAGCGACGAAGATTGGAACCTCGCCACACAAGCCTTACACGATGCTCTTAAGAAAAAAG
GAATTGAATACGGGATCAAAGAAGGGGACGGAGCCTTTTATGGTCCTAAAATTGAATTCAATATCAAGGATTCACTCGGA
AGGTTATGGCAATGCGGAACGGTTCAAATTGACTTCTCTATGCCGAACCGATTTGAACTCGATTTCACTGCCTCTGACGG
AAAAAAACATGCACCAGTCATGATCCATAGAGCCATCTATGGATCTCTCGAAAGGTTCATTGGAATCCTCATCGAACACT
TCGAAGGAAAATTTCCTTTGTGGCTGAACCCAACTCAAATTCGTGTCTTAACTGTGGCAGAAACTCATAGTGATTACGCG
AAGGAAGTGTATCAGGATTTAGTAATGCAAGGTTTCCGTGTCGAATTGGATGTTCGGAACGAAAAGATTGGAAGCAAAAT
TAGGGATTCCATCATGAAACGCAGTAGTTACACTCTCATCCTTGGGGATAAAGAAAAAGAAGCAGGAAGTATTTCGTATC
GCCGTATGGGAGAAGAAACGACTGCTACTGTTTCACGTGATGGATTTCTTTCTTTACTCAAAGGTGAACTCAACTAA

Upstream 100 bases:

>100_bases
GAAAGTTTTCAGAAACTAACAGAATCTCTCGAAACAGGAAAACTCTGGTAAAACTTTACTTTTCGGGAGAATGTGCGTGC
GAAAACCTGGGATTACACTT

Downstream 100 bases:

>100_bases
AGAAATTTCTTGACTAACACTGGTAGGGCTTTACGATTCCTTCCAGTGTCCAAGAGCCAAGCATATCCTACGATAAGGGA
ACCAGAAACTCTTTATCTGG

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS [H]

Number of amino acids: Translated: 638; Mature: 637

Protein sequence:

>638_residues
MAALTITLPDGSSKELESGKSFSDFVQNQLPFLKEKALAVVLSDGRTVDLSFVPTENTTVKFLTFDDKEGKEVFHHSSAH
LLGMAVQRLWPEARLTVGPVIENGPGFFFYDIDFGDLVITVDDLPKIEAEMAKIVKEDLTVKRWELSKEEAIEKFKKENE
PYKVELIQGFDAASVSLYGQGEWYDLCRGPHVARTGQLKAFKLTAISGAYWKGDSKNKQLTRIYGVSFPTKKLLDEYIFL
IEEAKKRDHRKLGKELDLFSFQDEAPGFPFWHPKGTVLWNTLASYIREECFRRGYQEIKTPAILNSSLWKKSGHWDNFKE
NMYFTDIDESEFAVKPMNCPGCCLIYKYHMHSYRELPLRFMELGNVHRHEMSGVLHGLFRVRAFTQDDAHIYAPLDKVES
EVEDIIDFTFDVYKKFGFTEFKTFIATRPEKSQGSDEDWNLATQALHDALKKKGIEYGIKEGDGAFYGPKIEFNIKDSLG
RLWQCGTVQIDFSMPNRFELDFTASDGKKHAPVMIHRAIYGSLERFIGILIEHFEGKFPLWLNPTQIRVLTVAETHSDYA
KEVYQDLVMQGFRVELDVRNEKIGSKIRDSIMKRSSYTLILGDKEKEAGSISYRRMGEETTATVSRDGFLSLLKGELN

Sequences:

>Translated_638_residues
MAALTITLPDGSSKELESGKSFSDFVQNQLPFLKEKALAVVLSDGRTVDLSFVPTENTTVKFLTFDDKEGKEVFHHSSAH
LLGMAVQRLWPEARLTVGPVIENGPGFFFYDIDFGDLVITVDDLPKIEAEMAKIVKEDLTVKRWELSKEEAIEKFKKENE
PYKVELIQGFDAASVSLYGQGEWYDLCRGPHVARTGQLKAFKLTAISGAYWKGDSKNKQLTRIYGVSFPTKKLLDEYIFL
IEEAKKRDHRKLGKELDLFSFQDEAPGFPFWHPKGTVLWNTLASYIREECFRRGYQEIKTPAILNSSLWKKSGHWDNFKE
NMYFTDIDESEFAVKPMNCPGCCLIYKYHMHSYRELPLRFMELGNVHRHEMSGVLHGLFRVRAFTQDDAHIYAPLDKVES
EVEDIIDFTFDVYKKFGFTEFKTFIATRPEKSQGSDEDWNLATQALHDALKKKGIEYGIKEGDGAFYGPKIEFNIKDSLG
RLWQCGTVQIDFSMPNRFELDFTASDGKKHAPVMIHRAIYGSLERFIGILIEHFEGKFPLWLNPTQIRVLTVAETHSDYA
KEVYQDLVMQGFRVELDVRNEKIGSKIRDSIMKRSSYTLILGDKEKEAGSISYRRMGEETTATVSRDGFLSLLKGELN
>Mature_637_residues
AALTITLPDGSSKELESGKSFSDFVQNQLPFLKEKALAVVLSDGRTVDLSFVPTENTTVKFLTFDDKEGKEVFHHSSAHL
LGMAVQRLWPEARLTVGPVIENGPGFFFYDIDFGDLVITVDDLPKIEAEMAKIVKEDLTVKRWELSKEEAIEKFKKENEP
YKVELIQGFDAASVSLYGQGEWYDLCRGPHVARTGQLKAFKLTAISGAYWKGDSKNKQLTRIYGVSFPTKKLLDEYIFLI
EEAKKRDHRKLGKELDLFSFQDEAPGFPFWHPKGTVLWNTLASYIREECFRRGYQEIKTPAILNSSLWKKSGHWDNFKEN
MYFTDIDESEFAVKPMNCPGCCLIYKYHMHSYRELPLRFMELGNVHRHEMSGVLHGLFRVRAFTQDDAHIYAPLDKVESE
VEDIIDFTFDVYKKFGFTEFKTFIATRPEKSQGSDEDWNLATQALHDALKKKGIEYGIKEGDGAFYGPKIEFNIKDSLGR
LWQCGTVQIDFSMPNRFELDFTASDGKKHAPVMIHRAIYGSLERFIGILIEHFEGKFPLWLNPTQIRVLTVAETHSDYAK
EVYQDLVMQGFRVELDVRNEKIGSKIRDSIMKRSSYTLILGDKEKEAGSISYRRMGEETTATVSRDGFLSLLKGELN

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI38202255, Length=621, Percent_Identity=40.4186795491143, Blast_Score=488, Evalue=1e-138,
Organism=Homo sapiens, GI38570111, Length=613, Percent_Identity=39.8042414355628, Blast_Score=481, Evalue=1e-135,
Organism=Homo sapiens, GI20070344, Length=651, Percent_Identity=36.405529953917, Blast_Score=424, Evalue=1e-118,
Organism=Escherichia coli, GI1788013, Length=612, Percent_Identity=44.281045751634, Blast_Score=556, Evalue=1e-159,
Organism=Escherichia coli, GI1786392, Length=153, Percent_Identity=25.4901960784314, Blast_Score=66, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71984184, Length=634, Percent_Identity=41.1671924290221, Blast_Score=503, Evalue=1e-142,
Organism=Caenorhabditis elegans, GI71984192, Length=634, Percent_Identity=41.1671924290221, Blast_Score=503, Evalue=1e-142,
Organism=Saccharomyces cerevisiae, GI6322112, Length=655, Percent_Identity=40.9160305343511, Blast_Score=488, Evalue=1e-138,
Organism=Saccharomyces cerevisiae, GI6322654, Length=393, Percent_Identity=37.6590330788804, Blast_Score=275, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24583839, Length=633, Percent_Identity=40.7582938388626, Blast_Score=493, Evalue=1e-139,
Organism=Drosophila melanogaster, GI24583842, Length=633, Percent_Identity=40.7582938388626, Blast_Score=492, Evalue=1e-139,
Organism=Drosophila melanogaster, GI28574141, Length=633, Percent_Identity=40.7582938388626, Blast_Score=492, Evalue=1e-139,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD [H]

EC number: =6.1.1.3 [H]

Molecular weight: Translated: 73146; Mature: 73015

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAALTITLPDGSSKELESGKSFSDFVQNQLPFLKEKALAVVLSDGRTVDLSFVPTENTTV
CEEEEEECCCCCCHHHHCCCCHHHHHHHCCCHHHCCEEEEEEECCCEEEEEEECCCCCEE
KFLTFDDKEGKEVFHHSSAHLLGMAVQRLWPEARLTVGPVIENGPGFFFYDIDFGDLVIT
EEEEECCCCCHHHHHCCCHHHHHHHHHHHCCCCCEEECCEEECCCCEEEEEECCCCEEEE
VDDLPKIEAEMAKIVKEDLTVKRWELSKEEAIEKFKKENEPYKVELIQGFDAASVSLYGQ
ECCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEC
GEWYDLCRGPHVARTGQLKAFKLTAISGAYWKGDSKNKQLTRIYGVSFPTKKLLDEYIFL
CCHHHHHCCCCCCCCCCEEEEEEEEECCCEECCCCCCCEEEEEEECCCCHHHHHHHHHHH
IEEAKKRDHRKLGKELDLFSFQDEAPGFPFWHPKGTVLWNTLASYIREECFRRGYQEIKT
HHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCC
PAILNSSLWKKSGHWDNFKENMYFTDIDESEFAVKPMNCPGCCLIYKYHMHSYRELPLRF
CHHHCHHHHHCCCCCCCCCCCCEEEECCCCCCEEECCCCCCEEEEEEHHHHHHHHHHHHH
MELGNVHRHEMSGVLHGLFRVRAFTQDDAHIYAPLDKVESEVEDIIDFTFDVYKKFGFTE
HHHCCCHHHHHHHHHHHHHHHHEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCHHH
FKTFIATRPEKSQGSDEDWNLATQALHDALKKKGIEYGIKEGDGAFYGPKIEFNIKDSLG
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCEEEEEEHHHHH
RLWQCGTVQIDFSMPNRFELDFTASDGKKHAPVMIHRAIYGSLERFIGILIEHFEGKFPL
HHEECCEEEEEECCCCEEEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCE
WLNPTQIRVLTVAETHSDYAKEVYQDLVMQGFRVELDVRNEKIGSKIRDSIMKRSSYTLI
EECCCEEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCCEEE
LGDKEKEAGSISYRRMGEETTATVSRDGFLSLLKGELN
EECCCCCCCCCHHHHCCCHHHEEECCCHHHHHHCCCCC
>Mature Secondary Structure 
AALTITLPDGSSKELESGKSFSDFVQNQLPFLKEKALAVVLSDGRTVDLSFVPTENTTV
EEEEEECCCCCCHHHHCCCCHHHHHHHCCCHHHCCEEEEEEECCCEEEEEEECCCCCEE
KFLTFDDKEGKEVFHHSSAHLLGMAVQRLWPEARLTVGPVIENGPGFFFYDIDFGDLVIT
EEEEECCCCCHHHHHCCCHHHHHHHHHHHCCCCCEEECCEEECCCCEEEEEECCCCEEEE
VDDLPKIEAEMAKIVKEDLTVKRWELSKEEAIEKFKKENEPYKVELIQGFDAASVSLYGQ
ECCCCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHCCCCEEEEEEECCCCCEEEEEEC
GEWYDLCRGPHVARTGQLKAFKLTAISGAYWKGDSKNKQLTRIYGVSFPTKKLLDEYIFL
CCHHHHHCCCCCCCCCCEEEEEEEEECCCEECCCCCCCEEEEEEECCCCHHHHHHHHHHH
IEEAKKRDHRKLGKELDLFSFQDEAPGFPFWHPKGTVLWNTLASYIREECFRRGYQEIKT
HHHHHHHHHHHHCCCCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHHCC
PAILNSSLWKKSGHWDNFKENMYFTDIDESEFAVKPMNCPGCCLIYKYHMHSYRELPLRF
CHHHCHHHHHCCCCCCCCCCCCEEEECCCCCCEEECCCCCCEEEEEEHHHHHHHHHHHHH
MELGNVHRHEMSGVLHGLFRVRAFTQDDAHIYAPLDKVESEVEDIIDFTFDVYKKFGFTE
HHHCCCHHHHHHHHHHHHHHHHEECCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCHHH
FKTFIATRPEKSQGSDEDWNLATQALHDALKKKGIEYGIKEGDGAFYGPKIEFNIKDSLG
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCEECCEEEEEEHHHHH
RLWQCGTVQIDFSMPNRFELDFTASDGKKHAPVMIHRAIYGSLERFIGILIEHFEGKFPL
HHEECCEEEEEECCCCEEEEEEECCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCE
WLNPTQIRVLTVAETHSDYAKEVYQDLVMQGFRVELDVRNEKIGSKIRDSIMKRSSYTLI
EECCCEEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHHCCCCEEE
LGDKEKEAGSISYRRMGEETTATVSRDGFLSLLKGELN
EECCCCCCCCCHHHHCCCHHHEEECCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA