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The map label for this gene is tetA [H]

Identifier: 183221683

GI number: 183221683

Start: 2377641

End: 2378921

Strand: Reverse

Name: tetA [H]

Synonym: LEPBI_I2308

Alternate gene names: 183221683

Gene position: 2378921-2377641 (Counterclockwise)

Preceding gene: 183221684

Following gene: 183221680

Centisome position: 66.09

GC content: 38.49

Gene sequence:

>1281_bases
ATGAGTCAGTCTCCTAAAAAAATCCAATTCATTTTATTTTTAATCGTTTTTACTGATATGATGGGATTTTCCCTTTTGTT
TCCTCTTTTTCCTAAAACATTAGAATTTTTTTTGGCAAAAGGAGATGATGTGTTCTTTCGCACATTTTATTCTGCAGCTA
ATCTTTTGTCCTTTGGTGGGGATACCAAATACACCTTTGTATTGTTTGGTGGAATTTTAGGGAGTATTTATAGTTTTTTA
CAATTTTTAGCTGCACCAGTATGGGGAAGATTATCTGATATTTTTGGTCGACGTGCGATCTTAGTCTTCACAACTCTTGG
AAACACATTAGGATACCTTTTATGGTTATTTTCATCGCAGTTTTGGATGTTTGTTTTGAGTCGCGTCATCACTGGTCTCA
TGGGTGGGAATTTATCAGTTGCCTCTGCGGCCATGGCCGACCAAACCGATGAAAAATCGAGAGCGGCAGGGATGGGACTT
TTAGGTGCCGGGATTGGACTTGGGTTTGTGATGGGTCCACTCCTTGGTGGGATTAGTTCCCAATGGAACTTCCTCGATTC
GTTTTACAAAGAGGGAAGTGTTGTGGTTTTTCCTGCTTCCGCATTTTTTGCGATCCTTGTTTCTCTTGTTTCCATGGTGC
TTGTATACTTGTTTTTACCAGCTAACAAACCGGAATCAGTTCCTGAAAAAGAGATCCATCCTTTTTTATCCTTAAAAAAA
ATTGAATCAAGGAATTTGGTTCGAATTTCGTTACTCAATCTTTTGTTTGTTTTAAGTTTTTCAGGATTCGAATTTGTTGT
GAATTTTTTTCTATCTGATACCTTTCAGTTTTCTCCCAAAGAAATTGGATTCACATTTTTGTACATTGGAATCGTCATCA
TACTTGTACAAGGTGGTGTAGTCAGACGATTGTCAGGAAAAATATCTGAAAAAACAATTTCATTATTTGGGGGAGTGGTT
GTTGTTTTTGGGATGGCACTCCTTGTGCTAATCGGTTCCAAGTTTTTTGGATTGTTTGTCTCTTTATTCTTTTTGGCCTT
CGGAAGTGCTCTTGTGAATCCAGGACTCAGTTCCTTTGCCTCCCTAGAAAGTGGAAAAGGAGATTTAGGTAGGTCTCTCG
GATTATTCCGTAGTTTTGGATCCCTCGGGAGAGCCGTGTCTCCCGTCGTCTTTTCGCTGTTATACTTTCAGAAAGGACCG
ATCTTAGCGTTTTTCATTTCATTTGTCCTACTTTGTGGGTTTACCATTTTACTTACGTTTCAAAAACAAAGATCGACTTA
A

Upstream 100 bases:

>100_bases
AAGCTAAATCAAAATCCTAATCAGAATAAATTGATGAAAATCATAAAAAGCCACAGAGTTTTCTGTGGCTTTTTTTATTT
GATTTCAGAGAGTAGCTCTG

Downstream 100 bases:

>100_bases
AGGAAGGATTCCAATTTTGCTCGAAGCATCGGGAACATCAATTTTTCGTCCAACTTCTCTCCCGTGAGGGAAGTGCGGAC
AAGGAAACTATCTCGAATTT

Product: MFS family transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: TetA(B); Metal-tetracycline/H(+) antiporter [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MSQSPKKIQFILFLIVFTDMMGFSLLFPLFPKTLEFFLAKGDDVFFRTFYSAANLLSFGGDTKYTFVLFGGILGSIYSFL
QFLAAPVWGRLSDIFGRRAILVFTTLGNTLGYLLWLFSSQFWMFVLSRVITGLMGGNLSVASAAMADQTDEKSRAAGMGL
LGAGIGLGFVMGPLLGGISSQWNFLDSFYKEGSVVVFPASAFFAILVSLVSMVLVYLFLPANKPESVPEKEIHPFLSLKK
IESRNLVRISLLNLLFVLSFSGFEFVVNFFLSDTFQFSPKEIGFTFLYIGIVIILVQGGVVRRLSGKISEKTISLFGGVV
VVFGMALLVLIGSKFFGLFVSLFFLAFGSALVNPGLSSFASLESGKGDLGRSLGLFRSFGSLGRAVSPVVFSLLYFQKGP
ILAFFISFVLLCGFTILLTFQKQRST

Sequences:

>Translated_426_residues
MSQSPKKIQFILFLIVFTDMMGFSLLFPLFPKTLEFFLAKGDDVFFRTFYSAANLLSFGGDTKYTFVLFGGILGSIYSFL
QFLAAPVWGRLSDIFGRRAILVFTTLGNTLGYLLWLFSSQFWMFVLSRVITGLMGGNLSVASAAMADQTDEKSRAAGMGL
LGAGIGLGFVMGPLLGGISSQWNFLDSFYKEGSVVVFPASAFFAILVSLVSMVLVYLFLPANKPESVPEKEIHPFLSLKK
IESRNLVRISLLNLLFVLSFSGFEFVVNFFLSDTFQFSPKEIGFTFLYIGIVIILVQGGVVRRLSGKISEKTISLFGGVV
VVFGMALLVLIGSKFFGLFVSLFFLAFGSALVNPGLSSFASLESGKGDLGRSLGLFRSFGSLGRAVSPVVFSLLYFQKGP
ILAFFISFVLLCGFTILLTFQKQRST
>Mature_425_residues
SQSPKKIQFILFLIVFTDMMGFSLLFPLFPKTLEFFLAKGDDVFFRTFYSAANLLSFGGDTKYTFVLFGGILGSIYSFLQ
FLAAPVWGRLSDIFGRRAILVFTTLGNTLGYLLWLFSSQFWMFVLSRVITGLMGGNLSVASAAMADQTDEKSRAAGMGLL
GAGIGLGFVMGPLLGGISSQWNFLDSFYKEGSVVVFPASAFFAILVSLVSMVLVYLFLPANKPESVPEKEIHPFLSLKKI
ESRNLVRISLLNLLFVLSFSGFEFVVNFFLSDTFQFSPKEIGFTFLYIGIVIILVQGGVVRRLSGKISEKTISLFGGVVV
VFGMALLVLIGSKFFGLFVSLFFLAFGSALVNPGLSSFASLESGKGDLGRSLGLFRSFGSLGRAVSPVVFSLLYFQKGPI
LAFFISFVLLCGFTILLTFQKQRST

Specific function: Resistance to tetracycline by an active tetracycline efflux. This is an energy-dependent process that decreases the accumulation of the antibiotic in whole cells. This protein functions as a metal-tetracycline/H(+) antiporter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Homo sapiens, GI225703104, Length=378, Percent_Identity=28.8359788359788, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI225703102, Length=378, Percent_Identity=28.8359788359788, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI39753965, Length=352, Percent_Identity=25.2840909090909, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI217416366, Length=427, Percent_Identity=23.1850117096019, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17551570, Length=423, Percent_Identity=28.3687943262411, Blast_Score=174, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI71996331, Length=349, Percent_Identity=26.3610315186246, Blast_Score=84, Evalue=9e-17,
Organism=Drosophila melanogaster, GI17738085, Length=377, Percent_Identity=32.8912466843501, Blast_Score=159, Evalue=4e-39,
Organism=Drosophila melanogaster, GI221513297, Length=254, Percent_Identity=29.5275590551181, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI21357569, Length=348, Percent_Identity=27.0114942528736, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24656463, Length=348, Percent_Identity=27.0114942528736, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24656468, Length=348, Percent_Identity=27.0114942528736, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI161080926, Length=348, Percent_Identity=27.0114942528736, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI161080924, Length=348, Percent_Identity=27.0114942528736, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46676; Mature: 46545

Theoretical pI: Translated: 10.28; Mature: 10.28

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCC
>Mature Secondary Structure 
SQSPKKIQFILFLIVFTDMMGFSLLFPLFPKTLEFFLAKGDDVFFRTFYSAANLLSFGG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
DTKYTFVLFGGILGSIYSFLQFLAAPVWGRLSDIFGRRAILVFTTLGNTLGYLLWLFSSQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHH
FWMFVLSRVITGLMGGNLSVASAAMADQTDEKSRAAGMGLLGAGIGLGFVMGPLLGGISS
HHHHHHHHHHHHHHCCCCHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHCCCH
QWNFLDSFYKEGSVVVFPASAFFAILVSLVSMVLVYLFLPANKPESVPEKEIHPFLSLKK
HHHHHHHHHCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHH
IESRNLVRISLLNLLFVLSFSGFEFVVNFFLSDTFQFSPKEIGFTFLYIGIVIILVQGGV
HCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCH
VRRLSGKISEKTISLFGGVVVVFGMALLVLIGSKFFGLFVSLFFLAFGSALVNPGLSSFA
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
SLESGKGDLGRSLGLFRSFGSLGRAVSPVVFSLLYFQKGPILAFFISFVLLCGFTILLTF
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
QKQRST
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 6319234; 6298728; 1848846; 2168416; 8995300 [H]