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The map label for this gene is thiC [H]

Identifier: 183221678

GI number: 183221678

Start: 2371675

End: 2373249

Strand: Reverse

Name: thiC [H]

Synonym: LEPBI_I2303

Alternate gene names: 183221678

Gene position: 2373249-2371675 (Counterclockwise)

Preceding gene: 183221679

Following gene: 183221673

Centisome position: 65.93

GC content: 43.94

Gene sequence:

>1575_bases
ATGAGTGATTCCGGCCAAGGAAGTCTCATGTCTCAAAACCATTCAAACCATTCGCCTATAAAGGAAATGTTTACACCAGA
AACGACCATTCCTTTGTCGGATGGAACCGAATACAAAACCTACCGCACAGAAGGGATGTATTGTATCCATGAAGAAACGT
ATGATTACAAACAAGGGATCCCGAAACTGAGAAAACCTTGGGTTGATGTTAGGGAAAGTAGGGGAGATCAAAATGTTTCC
CAACTCTATTATGCAAAAAGAAACATCATCACAGAAGAGATGTTATATGTTGCCAAACGTGAAGGTATGAGTCCTGAATT
TGTATTGAATGAAGTCAAAATTGGTCGTGCCATCATTCCATCTAACAAACGCCATTTGGAATTGGAACCCATGATCATTG
GAAAAAAGTTTCTAGTGAAAATCAATGCCAATATTGGAAATTCGGCCATTCTGTCTTCGATTGATGATGAAGTGGAAAAA
CTACGTTGGGCCTTACATTGGGGTGCCGATACCGTGATGGACCTCTCCACTGGCAAGAATATCCACGAAACAAGGGAATG
GATCATTCGGAATTCGCCAGTTCCCATTGGAACTGTTCCTTTGTACCAAACCTTGGAAAAGGTAAAGGGTAAAGTAGAAG
ATTTAAATATTGGAGTATTCCTCGAAACCTTGGAAGAGCAGGCAGAACAAGGTGTGGATTATTTTACCATCCATGCAGGG
GTGTTACGTGACTATGTCCGGCTCACAGAAAATCGAATCACAGGCATTGTTTCGAGGGGTGGTTCCATTTTGGCGAAGTG
GTGTAACCATCATAAAAAAGAAAACTTTTTGTATGAACATTTTGATGAAATCTCAAAAGTCATGCAAAAATATGGAGTTT
CTTACTCGTTAGGTGATGGGCTAAGGCCAGGTTGTATCAATGATGCCAATGACGCGGCACAATTTGCAGAACTCAAAACC
TTAGGGGAACTCACAAAACGTGCTTGGGCCTTTGACATCCAAGTGATGGTAGAGGGACCAGGCCACGTTCCCATGCACCT
CATCCAAGAAAATGTAAAGTTACAAGAAGAGATCTGTATGGAAGCACCGTTTTATACTCTTGGTCCCCTTGTCACAGACA
TCGCTCCTGGTTATGACCATATCACCTCTGCCATTGGTGCTGCCATGATTGCTTGGTACGGAACCGCTATGCTCTGTTAT
GTGACACCGAAAGAACATTTGGGTCTTCCCAATAAACAAGATGTGAAGGATGGAGTGATTGCTTATAAAATTGCAGCTCA
TGCCGCAGACCTTGCCAAGGGCCACCCAGGTGCGAAGGAACGAGATGATTTGCTGAGCAAAGCTCGGTTTGAATTTCGTT
GGGAAGACCAGTTTGCACTCTCACTCGATCCTGAACTGGCAAGGTCATACCACGACGAGTCCCTCCCGCAGGATGGAATG
AAAAAGGCACATTTCTGTTCGATGTGTGGGCCTCATTTTTGTTCGATGCGACTCACATCCGACTTACGGAAAGATACCGT
TGGTGTCAGCGGAGAAGTTGGTTCAAAGGAAAGAGGAGAGGAAGGAAAGGGATAG

Upstream 100 bases:

>100_bases
AAATATTCCTTAGTTTGCGTCAGTGGCCATGTTCATTCGAAATGGTTTTAGTCGACATTAACATTGGTTATACTTCTTTT
CCTTTCCATCCAAACCAGCG

Downstream 100 bases:

>100_bases
ATTGATCATTGGGTGGAGCAGTGCAAGAGGCAAAGGGAAACCATTTGCTTTTTCATATTTGGTCGACTAGGGTGCCAAAT
CGAGTGAAACCATAAAAAAA

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC [H]

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MSDSGQGSLMSQNHSNHSPIKEMFTPETTIPLSDGTEYKTYRTEGMYCIHEETYDYKQGIPKLRKPWVDVRESRGDQNVS
QLYYAKRNIITEEMLYVAKREGMSPEFVLNEVKIGRAIIPSNKRHLELEPMIIGKKFLVKINANIGNSAILSSIDDEVEK
LRWALHWGADTVMDLSTGKNIHETREWIIRNSPVPIGTVPLYQTLEKVKGKVEDLNIGVFLETLEEQAEQGVDYFTIHAG
VLRDYVRLTENRITGIVSRGGSILAKWCNHHKKENFLYEHFDEISKVMQKYGVSYSLGDGLRPGCINDANDAAQFAELKT
LGELTKRAWAFDIQVMVEGPGHVPMHLIQENVKLQEEICMEAPFYTLGPLVTDIAPGYDHITSAIGAAMIAWYGTAMLCY
VTPKEHLGLPNKQDVKDGVIAYKIAAHAADLAKGHPGAKERDDLLSKARFEFRWEDQFALSLDPELARSYHDESLPQDGM
KKAHFCSMCGPHFCSMRLTSDLRKDTVGVSGEVGSKERGEEGKG

Sequences:

>Translated_524_residues
MSDSGQGSLMSQNHSNHSPIKEMFTPETTIPLSDGTEYKTYRTEGMYCIHEETYDYKQGIPKLRKPWVDVRESRGDQNVS
QLYYAKRNIITEEMLYVAKREGMSPEFVLNEVKIGRAIIPSNKRHLELEPMIIGKKFLVKINANIGNSAILSSIDDEVEK
LRWALHWGADTVMDLSTGKNIHETREWIIRNSPVPIGTVPLYQTLEKVKGKVEDLNIGVFLETLEEQAEQGVDYFTIHAG
VLRDYVRLTENRITGIVSRGGSILAKWCNHHKKENFLYEHFDEISKVMQKYGVSYSLGDGLRPGCINDANDAAQFAELKT
LGELTKRAWAFDIQVMVEGPGHVPMHLIQENVKLQEEICMEAPFYTLGPLVTDIAPGYDHITSAIGAAMIAWYGTAMLCY
VTPKEHLGLPNKQDVKDGVIAYKIAAHAADLAKGHPGAKERDDLLSKARFEFRWEDQFALSLDPELARSYHDESLPQDGM
KKAHFCSMCGPHFCSMRLTSDLRKDTVGVSGEVGSKERGEEGKG
>Mature_523_residues
SDSGQGSLMSQNHSNHSPIKEMFTPETTIPLSDGTEYKTYRTEGMYCIHEETYDYKQGIPKLRKPWVDVRESRGDQNVSQ
LYYAKRNIITEEMLYVAKREGMSPEFVLNEVKIGRAIIPSNKRHLELEPMIIGKKFLVKINANIGNSAILSSIDDEVEKL
RWALHWGADTVMDLSTGKNIHETREWIIRNSPVPIGTVPLYQTLEKVKGKVEDLNIGVFLETLEEQAEQGVDYFTIHAGV
LRDYVRLTENRITGIVSRGGSILAKWCNHHKKENFLYEHFDEISKVMQKYGVSYSLGDGLRPGCINDANDAAQFAELKTL
GELTKRAWAFDIQVMVEGPGHVPMHLIQENVKLQEEICMEAPFYTLGPLVTDIAPGYDHITSAIGAAMIAWYGTAMLCYV
TPKEHLGLPNKQDVKDGVIAYKIAAHAADLAKGHPGAKERDDLLSKARFEFRWEDQFALSLDPELARSYHDESLPQDGMK
KAHFCSMCGPHFCSMRLTSDLRKDTVGVSGEVGSKERGEEGKG

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=542, Percent_Identity=58.8560885608856, Blast_Score=647, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 58944; Mature: 58813

Theoretical pI: Translated: 6.12; Mature: 6.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSGQGSLMSQNHSNHSPIKEMFTPETTIPLSDGTEYKTYRTEGMYCIHEETYDYKQGI
CCCCCCCCCCCCCCCCCCCHHHHCCCCCEEECCCCCCCEEEECCCEEEEECCCCHHHHCC
PKLRKPWVDVRESRGDQNVSQLYYAKRNIITEEMLYVAKREGMSPEFVLNEVKIGRAIIP
HHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEECC
SNKRHLELEPMIIGKKFLVKINANIGNSAILSSIDDEVEKLRWALHWGADTVMDLSTGKN
CCCCEEEECEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC
IHETREWIIRNSPVPIGTVPLYQTLEKVKGKVEDLNIGVFLETLEEQAEQGVDYFTIHAG
HHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHCCCCEEEEHHH
VLRDYVRLTENRITGIVSRGGSILAKWCNHHKKENFLYEHFDEISKVMQKYGVSYSLGDG
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEECCCC
LRPGCINDANDAAQFAELKTLGELTKRAWAFDIQVMVEGPGHVPMHLIQENVKLQEEICM
CCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHH
EAPFYTLGPLVTDIAPGYDHITSAIGAAMIAWYGTAMLCYVTPKEHLGLPNKQDVKDGVI
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCCCCHHCCHH
AYKIAAHAADLAKGHPGAKERDDLLSKARFEFRWEDQFALSLDPELARSYHDESLPQDGM
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHCEEEECCCEEEECCHHHHHHHCCCCCCHHHH
KKAHFCSMCGPHFCSMRLTSDLRKDTVGVSGEVGSKERGEEGKG
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SDSGQGSLMSQNHSNHSPIKEMFTPETTIPLSDGTEYKTYRTEGMYCIHEETYDYKQGI
CCCCCCCCCCCCCCCCCCHHHHCCCCCEEECCCCCCCEEEECCCEEEEECCCCHHHHCC
PKLRKPWVDVRESRGDQNVSQLYYAKRNIITEEMLYVAKREGMSPEFVLNEVKIGRAIIP
HHHHCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEECC
SNKRHLELEPMIIGKKFLVKINANIGNSAILSSIDDEVEKLRWALHWGADTVMDLSTGKN
CCCCEEEECEEEECCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCC
IHETREWIIRNSPVPIGTVPLYQTLEKVKGKVEDLNIGVFLETLEEQAEQGVDYFTIHAG
HHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHCCHHHHHHHHHHHHHCCCCEEEEHHH
VLRDYVRLTENRITGIVSRGGSILAKWCNHHKKENFLYEHFDEISKVMQKYGVSYSLGDG
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEECCCC
LRPGCINDANDAAQFAELKTLGELTKRAWAFDIQVMVEGPGHVPMHLIQENVKLQEEICM
CCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHH
EAPFYTLGPLVTDIAPGYDHITSAIGAAMIAWYGTAMLCYVTPKEHLGLPNKQDVKDGVI
CCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEEECCHHHCCCCCCCCHHCCHH
AYKIAAHAADLAKGHPGAKERDDLLSKARFEFRWEDQFALSLDPELARSYHDESLPQDGM
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHCEEEECCCEEEECCHHHHHHHCCCCCCHHHH
KKAHFCSMCGPHFCSMRLTSDLRKDTVGVSGEVGSKERGEEGKG
HHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA