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The map label for this gene is rpoA

Identifier: 183221322

GI number: 183221322

Start: 2028112

End: 2029089

Strand: Reverse

Name: rpoA

Synonym: LEPBI_I1939

Alternate gene names: 183221322

Gene position: 2029089-2028112 (Counterclockwise)

Preceding gene: 183221323

Following gene: 183221321

Centisome position: 56.37

GC content: 39.26

Gene sequence:

>978_bases
TTGTCTCCAAAGAATTTATTAAAAGGTTTTAAAAGACCCAAAAAAATCGAATTCACTACCGATGTGAATACACCAAACTA
CGGTAAGTTTGTTGCAGAACCTTTCGAAAGAGGAATTGGTACAACGATTGGGAACTCCCTTCGTCGTACACTTATGTCTT
CTATTGAAGGCGCGGCGATCTCCGCGATTCGGATTGAAGGAGTCTCACACGAGTTCTCTTATATTGAAGGTGTAGCAGAA
GACGTAACTCGTATCATTCTTAACTTAAAACAAGTTCGAATCAAATACGAGCCGGAAGACAAAGAAGCAAGCAAAGTGAT
CCACCTTGAATTAAAAGGTGCAGGTTACTTTCGTGCGGCTGACCTAGCAGTTGATTCTTCGATTGAAATCATGAACCCAG
ACCTTCATATTGCAACCCTCAATGAGGATGCCAATTTGATAATGGATTTGGAAATCCAAAGAGGACGTGGTTACGTTCCT
GCGGAAGACAAAAAGAAAGACATTGAAGTATTGGGAACGATTCCAATCGATTCTATCTTTTCACCGATTCAAAAAGTATT
GTTTGAAGTATCAGAAACTCGAGTCGCACAACGTTCTGATTACGAAAAATTGACGATGGAAGTTTGGACTGACGGTTCTG
TATCTCCAGAAGATGCAGTAGCGCAAGCAGCCAAAATTCTAAAAGACCACTTAACTGTTTTCATTAACTTTGAAGAAGAA
ATCGAAGAAGAAGAAGAAGAGTTGGATGAAGCTGATGAAAAACTGAAAGCAGCTTTATCAAAACACGTTGAAGAATTGGA
ACTTTCTGTTCGTTCTACAAACGTTCTTCGCAGTTTGGAAATTGACTTCATTGGTGAGCTCGTGAAGAGATCAGAAGACG
AAATGACGAAATCAAAACATTTCAGCGAACAAAGTTTGTTAGAGTTGAAAGCAAAACTTTCCTCTATGGGACTTTCGTTC
GGTATGAGAGATTTTTAA

Upstream 100 bases:

>100_bases
TTGTCACTTCCAACAAGAGATCATATTGATATCCCTGTGAAAGAACAGGTAATCGTAGAGTTGTACTCGAAGTAAGAATT
TAGAGAAGAAGGATACGACA

Downstream 100 bases:

>100_bases
GATGAATAAACGTAATAAAGTTAAACAACTCAACAGATCCGCTGATCATAGAAAAGCTATGATCCAAAATATGGTAATCT
CTTTACTTCGCCACGAAAGA

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 325; Mature: 324

Protein sequence:

>325_residues
MSPKNLLKGFKRPKKIEFTTDVNTPNYGKFVAEPFERGIGTTIGNSLRRTLMSSIEGAAISAIRIEGVSHEFSYIEGVAE
DVTRIILNLKQVRIKYEPEDKEASKVIHLELKGAGYFRAADLAVDSSIEIMNPDLHIATLNEDANLIMDLEIQRGRGYVP
AEDKKKDIEVLGTIPIDSIFSPIQKVLFEVSETRVAQRSDYEKLTMEVWTDGSVSPEDAVAQAAKILKDHLTVFINFEEE
IEEEEEELDEADEKLKAALSKHVEELELSVRSTNVLRSLEIDFIGELVKRSEDEMTKSKHFSEQSLLELKAKLSSMGLSF
GMRDF

Sequences:

>Translated_325_residues
MSPKNLLKGFKRPKKIEFTTDVNTPNYGKFVAEPFERGIGTTIGNSLRRTLMSSIEGAAISAIRIEGVSHEFSYIEGVAE
DVTRIILNLKQVRIKYEPEDKEASKVIHLELKGAGYFRAADLAVDSSIEIMNPDLHIATLNEDANLIMDLEIQRGRGYVP
AEDKKKDIEVLGTIPIDSIFSPIQKVLFEVSETRVAQRSDYEKLTMEVWTDGSVSPEDAVAQAAKILKDHLTVFINFEEE
IEEEEEELDEADEKLKAALSKHVEELELSVRSTNVLRSLEIDFIGELVKRSEDEMTKSKHFSEQSLLELKAKLSSMGLSF
GMRDF
>Mature_324_residues
SPKNLLKGFKRPKKIEFTTDVNTPNYGKFVAEPFERGIGTTIGNSLRRTLMSSIEGAAISAIRIEGVSHEFSYIEGVAED
VTRIILNLKQVRIKYEPEDKEASKVIHLELKGAGYFRAADLAVDSSIEIMNPDLHIATLNEDANLIMDLEIQRGRGYVPA
EDKKKDIEVLGTIPIDSIFSPIQKVLFEVSETRVAQRSDYEKLTMEVWTDGSVSPEDAVAQAAKILKDHLTVFINFEEEI
EEEEEELDEADEKLKAALSKHVEELELSVRSTNVLRSLEIDFIGELVKRSEDEMTKSKHFSEQSLLELKAKLSSMGLSFG
MRDF

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=322, Percent_Identity=38.1987577639752, Blast_Score=226, Evalue=1e-60,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_LEPBA (B0SA20)

Other databases:

- EMBL:   CP000777
- RefSeq:   YP_001962968.1
- ProteinModelPortal:   B0SA20
- GeneID:   6389814
- GenomeReviews:   CP000777_GR
- KEGG:   lbf:LBF_1886
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- BioCyc:   LBIF355278:LBF_1886-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 36600; Mature: 36469

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPKNLLKGFKRPKKIEFTTDVNTPNYGKFVAEPFERGIGTTIGNSLRRTLMSSIEGAAI
CCHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEE
SAIRIEGVSHEFSYIEGVAEDVTRIILNLKQVRIKYEPEDKEASKVIHLELKGAGYFRAA
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCEEEEEEEECCCCEEEE
DLAVDSSIEIMNPDLHIATLNEDANLIMDLEIQRGRGYVPAEDKKKDIEVLGTIPIDSIF
EEEECCCCEEECCCEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCEEEEECCCHHHHH
SPIQKVLFEVSETRVAQRSDYEKLTMEVWTDGSVSPEDAVAQAAKILKDHLTVFINFEEE
HHHHHHHHHHHHHHHHHHCCHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHEEEEECHHH
IEEEEEELDEADEKLKAALSKHVEELELSVRSTNVLRSLEIDFIGELVKRSEDEMTKSKH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHC
FSEQSLLELKAKLSSMGLSFGMRDF
CCHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SPKNLLKGFKRPKKIEFTTDVNTPNYGKFVAEPFERGIGTTIGNSLRRTLMSSIEGAAI
CHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCEE
SAIRIEGVSHEFSYIEGVAEDVTRIILNLKQVRIKYEPEDKEASKVIHLELKGAGYFRAA
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCEEEEEEEECCCCEEEE
DLAVDSSIEIMNPDLHIATLNEDANLIMDLEIQRGRGYVPAEDKKKDIEVLGTIPIDSIF
EEEECCCCEEECCCEEEEEECCCCCEEEEEEEECCCCCCCCCCCCCCEEEEECCCHHHHH
SPIQKVLFEVSETRVAQRSDYEKLTMEVWTDGSVSPEDAVAQAAKILKDHLTVFINFEEE
HHHHHHHHHHHHHHHHHHCCHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHEEEEECHHH
IEEEEEELDEADEKLKAALSKHVEELELSVRSTNVLRSLEIDFIGELVKRSEDEMTKSKH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHC
FSEQSLLELKAKLSSMGLSFGMRDF
CCHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA