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The map label for this gene is lpdA2 [H]

Identifier: 183221286

GI number: 183221286

Start: 1995133

End: 1996518

Strand: Direct

Name: lpdA2 [H]

Synonym: LEPBI_I1903

Alternate gene names: 183221286

Gene position: 1995133-1996518 (Clockwise)

Preceding gene: 183221285

Following gene: 183221287

Centisome position: 55.43

GC content: 42.93

Gene sequence:

>1386_bases
ATGAAAGAATATGACATCTTAGTGATCGGAGCGGGCGCTGGCACAAAACTTGTCACTCCCCCTTCCCAGATAGGCAAACG
AGTGGCAGTCTTTGAACGAGAAACCCCGGGTGGCACATGCCTGAACCGAGGTTGCATTCCCTCCAAAATGGTGATTTTTC
CCTCTGAGCTCCTTCGGCTAAAAGAGGAATCAGAACGATTTGGAATCCAATACCCAAGTCCCCCAATCTATGATGTAAAC
TCTATCTTCCAGCGAGTGAATGAAAGAGTAAAAGCTGATTCGGATTCCATTCCCATCGCTTATGAAAAAAATCCAAATAT
TGATTATATACCTAAAAACGTTTGGTTCAAAGCATCCAAAATCATCACCGATGGAGAAAACGATTACACCGCCAAACATA
TCTTGGTTGTCACAGGCACAAGGCCTAATCTCCCAGAAATCCCTGGATTAAAGGACACACCTTTTTGGACCTCCCGAGAA
GCCTTGTCCCCCGATGAATTCCCAAAATCACTCCTTATCATTGGAGCAGGTTTTATCTCACTTGAATTAGGCGCCGCCTA
CCAGGCATATGGTGCACAGGTGACAGGGATCACGAGAGGAGAAGTCTTACGGCAAGTTGATTTTGAAATCAAAGAGGAAC
TCAAGAAACACCTTCCCTTTCCCATCCACACTGGTTTTCATATGGAACAGGTGGAATTTCGAAATGGAGAGTTCCGCGTA
TCGGGAACAAACAAAGAAGGGCAAACACAAACATTTGTCGCAGAAAAATTATTGGTTGCGACAGGGATCAAACCGAACAC
GGAAGATTTAAAACTAGAGAATACCAAAATCCAATGTAACCCAGATGGTTACATCCTTGTGGATGAAACTTTACAAACAA
ACGAAGAAGGAGTTTATGCTTTTGGTGATGTGATCGGTCGGTATTTTTTCAGACACAGTGCCAATTTTGAAGGAGAATAC
TTATTCCACCATCTCTATGAGGGAGGTGAAAAAAAACCAATTGTTTACCCACCCATGCCGGAAGCAATCTTCACTCACCC
TCAAATCGCAAGTGTTGGAAAAACAGAAGATGAACTCATACAAGAAAAAATTCCTTATTACAAAGGTCTGAACCCGTATC
GTTCCAGTGCCACAGGTATGGCTCGTTTGTCTGAAGTTGGATTTGTGAAAGTCCTTGTTTCGAAAGAAACAGAAGAAGTG
CTTGGTGCCCATGTGATCGGCGAAGAAGCCGCAAACCTCCTCCACCAAATTGTAATGGGTATGTATTTAAAAGCGAAACT
CGATGATTATTTAGGAATGATTTACATCCACCCTGCCATTTCGGAAATCACAAGGAATGCCTTTCGTAAGGTTCGGGAAC
AAAAACTAAAGGAAATCAAATCATGA

Upstream 100 bases:

>100_bases
CTCAGATTTGTCACATTACGATGATTTCTGAAATCTAATTTTACCAAAACAAAGATAGCTTCATTTACAGAATCGGGCAA
GTTTGGAGTCTGGAATTGGA

Downstream 100 bases:

>100_bases
AAAAGTTTTTATTCAATCGTTACGATAAAGAAACTCTCAAAAAGAAAGTATTAGAAGACAAACGGGAAAGGAGAGTGATT
TCCTTTTATCGTTATGTGAA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Mycothiol-disulfide reductase; NADPH-dependent mycothione reductase [H]

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MKEYDILVIGAGAGTKLVTPPSQIGKRVAVFERETPGGTCLNRGCIPSKMVIFPSELLRLKEESERFGIQYPSPPIYDVN
SIFQRVNERVKADSDSIPIAYEKNPNIDYIPKNVWFKASKIITDGENDYTAKHILVVTGTRPNLPEIPGLKDTPFWTSRE
ALSPDEFPKSLLIIGAGFISLELGAAYQAYGAQVTGITRGEVLRQVDFEIKEELKKHLPFPIHTGFHMEQVEFRNGEFRV
SGTNKEGQTQTFVAEKLLVATGIKPNTEDLKLENTKIQCNPDGYILVDETLQTNEEGVYAFGDVIGRYFFRHSANFEGEY
LFHHLYEGGEKKPIVYPPMPEAIFTHPQIASVGKTEDELIQEKIPYYKGLNPYRSSATGMARLSEVGFVKVLVSKETEEV
LGAHVIGEEAANLLHQIVMGMYLKAKLDDYLGMIYIHPAISEITRNAFRKVREQKLKEIKS

Sequences:

>Translated_461_residues
MKEYDILVIGAGAGTKLVTPPSQIGKRVAVFERETPGGTCLNRGCIPSKMVIFPSELLRLKEESERFGIQYPSPPIYDVN
SIFQRVNERVKADSDSIPIAYEKNPNIDYIPKNVWFKASKIITDGENDYTAKHILVVTGTRPNLPEIPGLKDTPFWTSRE
ALSPDEFPKSLLIIGAGFISLELGAAYQAYGAQVTGITRGEVLRQVDFEIKEELKKHLPFPIHTGFHMEQVEFRNGEFRV
SGTNKEGQTQTFVAEKLLVATGIKPNTEDLKLENTKIQCNPDGYILVDETLQTNEEGVYAFGDVIGRYFFRHSANFEGEY
LFHHLYEGGEKKPIVYPPMPEAIFTHPQIASVGKTEDELIQEKIPYYKGLNPYRSSATGMARLSEVGFVKVLVSKETEEV
LGAHVIGEEAANLLHQIVMGMYLKAKLDDYLGMIYIHPAISEITRNAFRKVREQKLKEIKS
>Mature_461_residues
MKEYDILVIGAGAGTKLVTPPSQIGKRVAVFERETPGGTCLNRGCIPSKMVIFPSELLRLKEESERFGIQYPSPPIYDVN
SIFQRVNERVKADSDSIPIAYEKNPNIDYIPKNVWFKASKIITDGENDYTAKHILVVTGTRPNLPEIPGLKDTPFWTSRE
ALSPDEFPKSLLIIGAGFISLELGAAYQAYGAQVTGITRGEVLRQVDFEIKEELKKHLPFPIHTGFHMEQVEFRNGEFRV
SGTNKEGQTQTFVAEKLLVATGIKPNTEDLKLENTKIQCNPDGYILVDETLQTNEEGVYAFGDVIGRYFFRHSANFEGEY
LFHHLYEGGEKKPIVYPPMPEAIFTHPQIASVGKTEDELIQEKIPYYKGLNPYRSSATGMARLSEVGFVKVLVSKETEEV
LGAHVIGEEAANLLHQIVMGMYLKAKLDDYLGMIYIHPAISEITRNAFRKVREQKLKEIKS

Specific function: Catalyzes the NAD(P)H-dependent reduction of mycothione (the oxidized disulfide form of mycothiol) to mycothiol [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=474, Percent_Identity=31.2236286919831, Blast_Score=179, Evalue=4e-45,
Organism=Homo sapiens, GI50301238, Length=446, Percent_Identity=28.2511210762332, Blast_Score=151, Evalue=1e-36,
Organism=Homo sapiens, GI22035672, Length=450, Percent_Identity=26, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI291045266, Length=459, Percent_Identity=25.2723311546841, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI148277065, Length=462, Percent_Identity=25.1082251082251, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI33519430, Length=462, Percent_Identity=25.1082251082251, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI33519428, Length=462, Percent_Identity=25.1082251082251, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI33519426, Length=462, Percent_Identity=25.1082251082251, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI148277071, Length=462, Percent_Identity=25.1082251082251, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI291045268, Length=455, Percent_Identity=24.1758241758242, Blast_Score=89, Evalue=6e-18,
Organism=Escherichia coli, GI1786307, Length=447, Percent_Identity=27.0693512304251, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1789915, Length=437, Percent_Identity=26.3157894736842, Blast_Score=144, Evalue=2e-35,
Organism=Escherichia coli, GI87082354, Length=443, Percent_Identity=29.1196388261851, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI87081717, Length=476, Percent_Identity=25.2100840336134, Blast_Score=119, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI32565766, Length=466, Percent_Identity=30.0429184549356, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17557007, Length=470, Percent_Identity=25.3191489361702, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71983429, Length=438, Percent_Identity=24.8858447488584, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71983419, Length=461, Percent_Identity=25.5965292841649, Blast_Score=119, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI71982272, Length=477, Percent_Identity=25.3668763102725, Blast_Score=119, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6321091, Length=470, Percent_Identity=28.936170212766, Blast_Score=156, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6325166, Length=467, Percent_Identity=27.8372591006424, Blast_Score=137, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6325240, Length=474, Percent_Identity=25.3164556962025, Blast_Score=104, Evalue=3e-23,
Organism=Drosophila melanogaster, GI21358499, Length=465, Percent_Identity=32.0430107526882, Blast_Score=191, Evalue=1e-48,
Organism=Drosophila melanogaster, GI24640549, Length=477, Percent_Identity=27.0440251572327, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24640553, Length=477, Percent_Identity=27.0440251572327, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24640551, Length=477, Percent_Identity=27.0440251572327, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI17737741, Length=470, Percent_Identity=24.468085106383, Blast_Score=113, Evalue=2e-25,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR017817
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.15 [H]

Molecular weight: Translated: 51822; Mature: 51822

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEYDILVIGAGAGTKLVTPPSQIGKRVAVFERETPGGTCLNRGCIPSKMVIFPSELLRL
CCCEEEEEEECCCCCCEECCHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEECCHHHHHH
KEESERFGIQYPSPPIYDVNSIFQRVNERVKADSDSIPIAYEKNPNIDYIPKNVWFKASK
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCEEEEEE
IITDGENDYTAKHILVVTGTRPNLPEIPGLKDTPFWTSREALSPDEFPKSLLIIGAGFIS
EEECCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCEEEEEECCEEE
LELGAAYQAYGAQVTGITRGEVLRQVDFEIKEELKKHLPFPIHTGFHMEQVEFRNGEFRV
EEECCHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEE
SGTNKEGQTQTFVAEKLLVATGIKPNTEDLKLENTKIQCNPDGYILVDETLQTNEEGVYA
ECCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCEEEECCCCEEEECCCCCCCCCCEEH
FGDVIGRYFFRHSANFEGEYLFHHLYEGGEKKPIVYPPMPEAIFTHPQIASVGKTEDELI
HHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEECCCCCHHHHCCCCHHCCCCCHHHHH
QEKIPYYKGLNPYRSSATGMARLSEVGFVKVLVSKETEEVLGAHVIGEEAANLLHQIVMG
HHHCCHHCCCCHHHHCHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
MYLKAKLDDYLGMIYIHPAISEITRNAFRKVREQKLKEIKS
HHHHHHHHHHCEEEEECHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKEYDILVIGAGAGTKLVTPPSQIGKRVAVFERETPGGTCLNRGCIPSKMVIFPSELLRL
CCCEEEEEEECCCCCCEECCHHHHCCEEEEEECCCCCCCCCCCCCCCCEEEECCHHHHHH
KEESERFGIQYPSPPIYDVNSIFQRVNERVKADSDSIPIAYEKNPNIDYIPKNVWFKASK
HHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCEEEEEE
IITDGENDYTAKHILVVTGTRPNLPEIPGLKDTPFWTSREALSPDEFPKSLLIIGAGFIS
EEECCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCEEEEEECCEEE
LELGAAYQAYGAQVTGITRGEVLRQVDFEIKEELKKHLPFPIHTGFHMEQVEFRNGEFRV
EEECCHHHHCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEE
SGTNKEGQTQTFVAEKLLVATGIKPNTEDLKLENTKIQCNPDGYILVDETLQTNEEGVYA
ECCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEECCEEEECCCCEEEECCCCCCCCCCEEH
FGDVIGRYFFRHSANFEGEYLFHHLYEGGEKKPIVYPPMPEAIFTHPQIASVGKTEDELI
HHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCEECCCCCHHHHCCCCHHCCCCCHHHHH
QEKIPYYKGLNPYRSSATGMARLSEVGFVKVLVSKETEEVLGAHVIGEEAANLLHQIVMG
HHHCCHHCCCCHHHHCHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHH
MYLKAKLDDYLGMIYIHPAISEITRNAFRKVREQKLKEIKS
HHHHHHHHHHCEEEEECHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]