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The map label for this gene is gloB [H]

Identifier: 183221283

GI number: 183221283

Start: 1993023

End: 1993799

Strand: Direct

Name: gloB [H]

Synonym: LEPBI_I1900

Alternate gene names: 183221283

Gene position: 1993023-1993799 (Clockwise)

Preceding gene: 183221282

Following gene: 183221284

Centisome position: 55.37

GC content: 40.67

Gene sequence:

>777_bases
ATGATGGAAGTCCTTCCCATTTTTACGAACTCACCCTTACGCAATTACACCTATCTCATTTACTCAAATCGAACAGGCGA
AGTGTATTGTGTGGATCCTTATAACGCTCCACTCCTTCTCGATTTGATGAAAAAAATGGGTTTAAAACTCAAAGGGATTT
TGAACACACATGAACATGGGGACCACACAGAAGGGAATTTAGAACTGAAAGAGGCAACAAATTGTCTTGTGTATGGACAT
ACAAAGGCAAAAAATACCATCCCTGGCCTTGATACAACTCTGAAAGAAGGTGACATCTGTTTTTCGACCGAAGGCGAAAG
TATTGAGGTTTGGGACACTCCTGGCCATACATTTTCCCATTTGAGTTTTGTTCATAAAAATCCAAACACCATCCTTGGGA
TTTTTTCAGGGGATACCTTATTCAATGTCGGTGTGGGAAATTGTTTTCGAGGTGGTGATCCGAATACCCTTTACGAAACC
ATAAAGAATCGATATGCCACTCTTCCCGATTCCTGTCTTTTGTATCCAGGGCATGACTACTGGGAAAATAATTTGGCATT
TTCAAATCACGTGGAACCAAACCATGATTTTCGCGACCGGTTCCAAAAAACAAGGACCGAACACTTCCAATCCACTTTAG
GATTGGAAAGGCAATTGAGTCCTTTTTTTCGACTTGGGTCTGGAGGAATAAAAGAGCGTCTAACAGAATTAGGTGAATCT
TTCACTGATGATAGGTCCATTTTTTTACGCTTACGAAAACTAAGGGATCGTTGGTAA

Upstream 100 bases:

>100_bases
CAAAAACCTGGGTGATGAATTGAAAGATAAAATCAAAGCCCACAAACATTCGATTCGCACTCGAATTCTCTACTAACCAA
CCGGGAAGGATCAGATCATC

Downstream 100 bases:

>100_bases
AATTTTCATTTTACATTTTTAAAATCTCCTTTTAGGATTTGTTATATGACAATTCTCATCATTTGCCTTTTGGTTTCAAT
CGCCCAAATCTACATTGCAA

Product: putative hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MMEVLPIFTNSPLRNYTYLIYSNRTGEVYCVDPYNAPLLLDLMKKMGLKLKGILNTHEHGDHTEGNLELKEATNCLVYGH
TKAKNTIPGLDTTLKEGDICFSTEGESIEVWDTPGHTFSHLSFVHKNPNTILGIFSGDTLFNVGVGNCFRGGDPNTLYET
IKNRYATLPDSCLLYPGHDYWENNLAFSNHVEPNHDFRDRFQKTRTEHFQSTLGLERQLSPFFRLGSGGIKERLTELGES
FTDDRSIFLRLRKLRDRW

Sequences:

>Translated_258_residues
MMEVLPIFTNSPLRNYTYLIYSNRTGEVYCVDPYNAPLLLDLMKKMGLKLKGILNTHEHGDHTEGNLELKEATNCLVYGH
TKAKNTIPGLDTTLKEGDICFSTEGESIEVWDTPGHTFSHLSFVHKNPNTILGIFSGDTLFNVGVGNCFRGGDPNTLYET
IKNRYATLPDSCLLYPGHDYWENNLAFSNHVEPNHDFRDRFQKTRTEHFQSTLGLERQLSPFFRLGSGGIKERLTELGES
FTDDRSIFLRLRKLRDRW
>Mature_258_residues
MMEVLPIFTNSPLRNYTYLIYSNRTGEVYCVDPYNAPLLLDLMKKMGLKLKGILNTHEHGDHTEGNLELKEATNCLVYGH
TKAKNTIPGLDTTLKEGDICFSTEGESIEVWDTPGHTFSHLSFVHKNPNTILGIFSGDTLFNVGVGNCFRGGDPNTLYET
IKNRYATLPDSCLLYPGHDYWENNLAFSNHVEPNHDFRDRFQKTRTEHFQSTLGLERQLSPFFRLGSGGIKERLTELGES
FTDDRSIFLRLRKLRDRW

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI116642887, Length=272, Percent_Identity=35.2941176470588, Blast_Score=115, Evalue=3e-26,
Organism=Homo sapiens, GI21703352, Length=272, Percent_Identity=35.2941176470588, Blast_Score=115, Evalue=4e-26,
Organism=Homo sapiens, GI94538322, Length=273, Percent_Identity=29.6703296703297, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI94538320, Length=273, Percent_Identity=29.6703296703297, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI14150041, Length=240, Percent_Identity=29.5833333333333, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI46361987, Length=197, Percent_Identity=29.9492385786802, Blast_Score=83, Evalue=3e-16,
Organism=Homo sapiens, GI41327741, Length=172, Percent_Identity=33.1395348837209, Blast_Score=78, Evalue=7e-15,
Organism=Escherichia coli, GI1786406, Length=219, Percent_Identity=29.6803652968037, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787158, Length=180, Percent_Identity=31.6666666666667, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17536925, Length=238, Percent_Identity=31.0924369747899, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17538952, Length=168, Percent_Identity=29.7619047619048, Blast_Score=74, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6320478, Length=269, Percent_Identity=27.1375464684015, Blast_Score=83, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24667711, Length=266, Percent_Identity=29.3233082706767, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI21356335, Length=267, Percent_Identity=29.9625468164794, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI24667703, Length=266, Percent_Identity=29.3233082706767, Blast_Score=112, Evalue=2e-25,
Organism=Drosophila melanogaster, GI221330176, Length=171, Percent_Identity=35.672514619883, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 29542; Mature: 29542

Theoretical pI: Translated: 6.58; Mature: 6.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMEVLPIFTNSPLRNYTYLIYSNRTGEVYCVDPYNAPLLLDLMKKMGLKLKGILNTHEHG
CCEEEEECCCCCCCCEEEEEEECCCCCEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCC
DHTEGNLELKEATNCLVYGHTKAKNTIPGLDTTLKEGDICFSTEGESIEVWDTPGHTFSH
CCCCCCEEHHHCCCEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCEEEEECCCCCHHHH
LSFVHKNPNTILGIFSGDTLFNVGVGNCFRGGDPNTLYETIKNRYATLPDSCLLYPGHDY
EEEEECCCCEEEEEEECCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCC
WENNLAFSNHVEPNHDFRDRFQKTRTEHFQSTLGLERQLSPFFRLGSGGIKERLTELGES
CCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHCCHHHHCCCCCHHHHHHHHHHH
FTDDRSIFLRLRKLRDRW
CCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMEVLPIFTNSPLRNYTYLIYSNRTGEVYCVDPYNAPLLLDLMKKMGLKLKGILNTHEHG
CCEEEEECCCCCCCCEEEEEEECCCCCEEEECCCCCCHHHHHHHHCCCEEEEEECCCCCC
DHTEGNLELKEATNCLVYGHTKAKNTIPGLDTTLKEGDICFSTEGESIEVWDTPGHTFSH
CCCCCCEEHHHCCCEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCEEEEECCCCCHHHH
LSFVHKNPNTILGIFSGDTLFNVGVGNCFRGGDPNTLYETIKNRYATLPDSCLLYPGHDY
EEEEECCCCEEEEEEECCEEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCC
WENNLAFSNHVEPNHDFRDRFQKTRTEHFQSTLGLERQLSPFFRLGSGGIKERLTELGES
CCCCCEECCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHCCHHHHCCCCCHHHHHHHHHHH
FTDDRSIFLRLRKLRDRW
CCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA