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The map label for this gene is puuP [H]

Identifier: 183221270

GI number: 183221270

Start: 1980395

End: 1981846

Strand: Direct

Name: puuP [H]

Synonym: LEPBI_I1887

Alternate gene names: 183221270

Gene position: 1980395-1981846 (Clockwise)

Preceding gene: 183221268

Following gene: 183221272

Centisome position: 55.02

GC content: 40.7

Gene sequence:

>1452_bases
ATGAGCCAAACTTCCCTAGACCACGATGCAGAACAATTAAAAGCACTCGGCTTAAAATCAGAATTTGATCGGAGTATGAG
TTTCTGGGAAAATTTTTCCTTAGGATTTACGTATCTTTCTCCCGTCGTCGGAGTGTATTCCGTATTTGCACTTGCCATCC
AAGCAGGTGGACCTCCGATGATTTGGAATTATATCCTGGTCGGTTTCGGACAATTTTTAGTCTGTTTGGTATTTGGTGAA
GTGGTCTCTCAATACCCGATCTCTGGTGGAATTTATCCTTGGTCACTGCGTCTTGTGGGAGACCGATTTGCATGGATGTC
TGCCTGGGTGTATGCTTGGGCTTTGTTCACATCTGTTGCCGCCGTAGCCGTAGGTGGGGCACCTTTTTTAAATAACTTAC
TCGGAATCGAACTTGGGAATACAGGTTTTATTTGGATTGCAATCGGAATGATTATTATATCCACATTACTAAATTTAAGT
GGAACGAAATTACTGGCACAAGTTGCTTTTTTTGGTTTTATCTGTGAACTCGTAGGTGCCATCATTGTAGGTGCGTCTCT
TTTATTCTTTGCAAAAGTAAATTCTATCTCCATCCTATGGAATACCTTTTCCTTTGGCGATGGCATGAGTTATTTTCCAG
CATTTCTTGCATCATCCGTTGCCGCTATGTTTTGTTATTATGGATTTGAAGCCTGCGGTGATGTGGCAGAAGAAACACCA
AATGCAAGTTCAGCGATTCCAAAGTCAATGAGAATGACGATATACATTGGAGGCGGTGCGGCCATTTTCATATGTTTGGC
ACTTCTATTATCCCTTCCGAATGTTGAAAAAGCCATTTCCGGAGAAGATGCAGATCCTGTCACAACAACATTGACTCATG
CCATGGGTGTGACAGGTTACAGAATGGTAATTGTCATCGTGATGATTTCGTTTTTGTCTTGTTTACTCAGCTTACAAGCG
GCTGCAAGCCGGCTTCTTTTTTCTTTTGCTCGGGATGGAATGATTTTTGGCAGTCAGTATTTAAACCATCTTTCCAAAAA
AGGAAAGGTTCCAACGAAAGCACTCCTTGTCACAGGAATCATTCCCATCATCATCACAAGCATTGGCCATTGGTTACAAG
ATGCTGTCATCACGATCATTAGTTTTGCTTCCTGTGGTATTTATGTTGCGTTCCAAATGGTTGTGTTTGGTGCATTGTAT
GCAAGGATCCGAGGTTGGAAACCAAAAGGTTCATTTACCTTAGGCAAATATGGATTTGTGATCAATGTATTAGCTTTGGT
TTACGGGACACTTGCAGTTTCAAATATGGTCTGGCCTAGATCACCTGACGAACCTTGGTTTATCAATTATGGAATGATTT
TTACTTCATTAATTGTCTTAATTACTGGCATTTTTTACTTACTCGTTGCCAAACCTCATTTGCGAAAAAAATATGCAAAA
AACATTTCATAG

Upstream 100 bases:

>100_bases
TTAAATGATTCTTTCGTCAATGGAAAAACAAAATCGTTTGTAGAAAAGAGACCCAATTATAAAACCAAACCAATCACAAA
ATGAAGGAATCGGTATTGGA

Downstream 100 bases:

>100_bases
TTAAAAGCGGCTTGGGAAAGTATGGAATCAGATCCTTTCCCATACCATCCACTCCCCCAATGGAAGTTCTTTTTTGACTA
AAACACGGAAACCTGGCAAA

Product: putative amino acid permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 483; Mature: 482

Protein sequence:

>483_residues
MSQTSLDHDAEQLKALGLKSEFDRSMSFWENFSLGFTYLSPVVGVYSVFALAIQAGGPPMIWNYILVGFGQFLVCLVFGE
VVSQYPISGGIYPWSLRLVGDRFAWMSAWVYAWALFTSVAAVAVGGAPFLNNLLGIELGNTGFIWIAIGMIIISTLLNLS
GTKLLAQVAFFGFICELVGAIIVGASLLFFAKVNSISILWNTFSFGDGMSYFPAFLASSVAAMFCYYGFEACGDVAEETP
NASSAIPKSMRMTIYIGGGAAIFICLALLLSLPNVEKAISGEDADPVTTTLTHAMGVTGYRMVIVIVMISFLSCLLSLQA
AASRLLFSFARDGMIFGSQYLNHLSKKGKVPTKALLVTGIIPIIITSIGHWLQDAVITIISFASCGIYVAFQMVVFGALY
ARIRGWKPKGSFTLGKYGFVINVLALVYGTLAVSNMVWPRSPDEPWFINYGMIFTSLIVLITGIFYLLVAKPHLRKKYAK
NIS

Sequences:

>Translated_483_residues
MSQTSLDHDAEQLKALGLKSEFDRSMSFWENFSLGFTYLSPVVGVYSVFALAIQAGGPPMIWNYILVGFGQFLVCLVFGE
VVSQYPISGGIYPWSLRLVGDRFAWMSAWVYAWALFTSVAAVAVGGAPFLNNLLGIELGNTGFIWIAIGMIIISTLLNLS
GTKLLAQVAFFGFICELVGAIIVGASLLFFAKVNSISILWNTFSFGDGMSYFPAFLASSVAAMFCYYGFEACGDVAEETP
NASSAIPKSMRMTIYIGGGAAIFICLALLLSLPNVEKAISGEDADPVTTTLTHAMGVTGYRMVIVIVMISFLSCLLSLQA
AASRLLFSFARDGMIFGSQYLNHLSKKGKVPTKALLVTGIIPIIITSIGHWLQDAVITIISFASCGIYVAFQMVVFGALY
ARIRGWKPKGSFTLGKYGFVINVLALVYGTLAVSNMVWPRSPDEPWFINYGMIFTSLIVLITGIFYLLVAKPHLRKKYAK
NIS
>Mature_482_residues
SQTSLDHDAEQLKALGLKSEFDRSMSFWENFSLGFTYLSPVVGVYSVFALAIQAGGPPMIWNYILVGFGQFLVCLVFGEV
VSQYPISGGIYPWSLRLVGDRFAWMSAWVYAWALFTSVAAVAVGGAPFLNNLLGIELGNTGFIWIAIGMIIISTLLNLSG
TKLLAQVAFFGFICELVGAIIVGASLLFFAKVNSISILWNTFSFGDGMSYFPAFLASSVAAMFCYYGFEACGDVAEETPN
ASSAIPKSMRMTIYIGGGAAIFICLALLLSLPNVEKAISGEDADPVTTTLTHAMGVTGYRMVIVIVMISFLSCLLSLQAA
ASRLLFSFARDGMIFGSQYLNHLSKKGKVPTKALLVTGIIPIIITSIGHWLQDAVITIISFASCGIYVAFQMVVFGALYA
RIRGWKPKGSFTLGKYGFVINVLALVYGTLAVSNMVWPRSPDEPWFINYGMIFTSLIVLITGIFYLLVAKPHLRKKYAKN
IS

Specific function: Imports putrescine [H]

COG id: COG0531

COG function: function code E; Amino acid transporters

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amino acid-polyamine-organocation (APC) superfamily [H]

Homologues:

Organism=Escherichia coli, GI87081869, Length=358, Percent_Identity=22.6256983240223, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1786302, Length=365, Percent_Identity=25.7534246575342, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI87082023, Length=385, Percent_Identity=20.2597402597403, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1786789, Length=334, Percent_Identity=24.8502994011976, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17532491, Length=363, Percent_Identity=23.6914600550964, Blast_Score=71, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322674, Length=486, Percent_Identity=21.6049382716049, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004841
- InterPro:   IPR002293 [H]

Pfam domain/function: PF00324 AA_permease [H]

EC number: NA

Molecular weight: Translated: 52471; Mature: 52339

Theoretical pI: Translated: 8.59; Mature: 8.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQTSLDHDAEQLKALGLKSEFDRSMSFWENFSLGFTYLSPVVGVYSVFALAIQAGGPPM
CCCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
IWNYILVGFGQFLVCLVFGEVVSQYPISGGIYPWSLRLVGDRFAWMSAWVYAWALFTSVA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
AVAVGGAPFLNNLLGIELGNTGFIWIAIGMIIISTLLNLSGTKLLAQVAFFGFICELVGA
HHHCCCCHHHHHHHCEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
IIVGASLLFFAKVNSISILWNTFSFGDGMSYFPAFLASSVAAMFCYYGFEACGDVAEETP
HHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NASSAIPKSMRMTIYIGGGAAIFICLALLLSLPNVEKAISGEDADPVTTTLTHAMGVTGY
CCCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHCCCHH
RMVIVIVMISFLSCLLSLQAAASRLLFSFARDGMIFGSQYLNHLSKKGKVPTKALLVTGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
IPIIITSIGHWLQDAVITIISFASCGIYVAFQMVVFGALYARIRGWKPKGSFTLGKYGFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECHHHHHH
INVLALVYGTLAVSNMVWPRSPDEPWFINYGMIFTSLIVLITGIFYLLVAKPHLRKKYAK
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHC
NIS
CCC
>Mature Secondary Structure 
SQTSLDHDAEQLKALGLKSEFDRSMSFWENFSLGFTYLSPVVGVYSVFALAIQAGGPPM
CCCCCCCCHHHHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHH
IWNYILVGFGQFLVCLVFGEVVSQYPISGGIYPWSLRLVGDRFAWMSAWVYAWALFTSVA
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
AVAVGGAPFLNNLLGIELGNTGFIWIAIGMIIISTLLNLSGTKLLAQVAFFGFICELVGA
HHHCCCCHHHHHHHCEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
IIVGASLLFFAKVNSISILWNTFSFGDGMSYFPAFLASSVAAMFCYYGFEACGDVAEETP
HHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
NASSAIPKSMRMTIYIGGGAAIFICLALLLSLPNVEKAISGEDADPVTTTLTHAMGVTGY
CCCCCCCCCEEEEEEECCHHHHHHHHHHHHCCCCHHHHHCCCCCCCHHHHHHHHHCCCHH
RMVIVIVMISFLSCLLSLQAAASRLLFSFARDGMIFGSQYLNHLSKKGKVPTKALLVTGI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHH
IPIIITSIGHWLQDAVITIISFASCGIYVAFQMVVFGALYARIRGWKPKGSFTLGKYGFV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECHHHHHH
INVLALVYGTLAVSNMVWPRSPDEPWFINYGMIFTSLIVLITGIFYLLVAKPHLRKKYAK
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHC
NIS
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]